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The map label for this gene is greB [H]

Identifier: 197117113

GI number: 197117113

Start: 850476

End: 850952

Strand: Direct

Name: greB [H]

Synonym: Gbem_0718

Alternate gene names: 197117113

Gene position: 850476-850952 (Clockwise)

Preceding gene: 197117112

Following gene: 197117115

Centisome position: 18.43

GC content: 61.84

Gene sequence:

>477_bases
ATGGTAAGCAACATGATCAGCGCGGAAGGGCGCAAACGACTGCAGGAGCGCTACGACTTCCTGTGGGAGACGGAGCGGCC
GCGCATGGTTAAGAATATGGCGGACGCGGCTGCGGAAGGGGACCGCTCCGAAAATGCCGAGTACATCTATTCGAAGAAGA
GGCTGCGCGAGATCGATCGGGAACTGAGGCACTTGGGGGACCGGCTTAAGGTGGTGAAGGTGGTTTACCCGCCGCTGAAC
CCGTCCACGGTGAGCTTCGGCTGCTGGGTCAGCTACGAGGACGAGGAAGGGGAGGAACGCTGCTATCAGCTGGTGGGACC
CGACGAGTTCGACGTAACCGCGGGAAAGATCAGCGTCGACTCGCCGGTCGGCAAGGCTCTTCTGGGGCGCAAGGTCGACG
ACGAGATCACCATCAAGCGCCCCGCCGGGGACCTGACCGTAACCATTGTCGCCATCAGTTCCAGCAGGCCCGGGTAA

Upstream 100 bases:

>100_bases
CCCCTTCTCCGCCCGAACAGTGAGCGGCAAAGGGGGCCGCGTTTCGGGCTCAGGGACTGGCGCCTTCTGGCGACTAACGG
TTAACAAGGAAGTGGAAATA

Downstream 100 bases:

>100_bases
CGGGCTACCCCTTGTTCCTTTTCTTCCGCTTCTTCTCCACCAGGGCGTAGATGGTTTCCACCGACCTGAAGTTGTCGAGC
GCCATGTCCTCGTCGCCGAT

Product: transcription elongation factor GreA/GreB domain-containing protein

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MVSNMISAEGRKRLQERYDFLWETERPRMVKNMADAAAEGDRSENAEYIYSKKRLREIDRELRHLGDRLKVVKVVYPPLN
PSTVSFGCWVSYEDEEGEERCYQLVGPDEFDVTAGKISVDSPVGKALLGRKVDDEITIKRPAGDLTVTIVAISSSRPG

Sequences:

>Translated_158_residues
MVSNMISAEGRKRLQERYDFLWETERPRMVKNMADAAAEGDRSENAEYIYSKKRLREIDRELRHLGDRLKVVKVVYPPLN
PSTVSFGCWVSYEDEEGEERCYQLVGPDEFDVTAGKISVDSPVGKALLGRKVDDEITIKRPAGDLTVTIVAISSSRPG
>Mature_158_residues
MVSNMISAEGRKRLQERYDFLWETERPRMVKNMADAAAEGDRSENAEYIYSKKRLREIDRELRHLGDRLKVVKVVYPPLN
PSTVSFGCWVSYEDEEGEERCYQLVGPDEFDVTAGKISVDSPVGKALLGRKVDDEITIKRPAGDLTVTIVAISSSRPG

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=158, Percent_Identity=40.5063291139241, Blast_Score=123, Evalue=6e-30,
Organism=Escherichia coli, GI87082225, Length=155, Percent_Identity=31.6129032258064, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17867; Mature: 17867

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSNMISAEGRKRLQERYDFLWETERPRMVKNMADAAAEGDRSENAEYIYSKKRLREIDR
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
ELRHLGDRLKVVKVVYPPLNPSTVSFGCWVSYEDEEGEERCYQLVGPDEFDVTAGKISVD
HHHHHHHHEEEEEEEECCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCCCCEEECEEECC
SPVGKALLGRKVDDEITIKRPAGDLTVTIVAISSSRPG
CCHHHHHHCCCCCCCEEEECCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MVSNMISAEGRKRLQERYDFLWETERPRMVKNMADAAAEGDRSENAEYIYSKKRLREIDR
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
ELRHLGDRLKVVKVVYPPLNPSTVSFGCWVSYEDEEGEERCYQLVGPDEFDVTAGKISVD
HHHHHHHHEEEEEEEECCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCCCCEEECEEECC
SPVGKALLGRKVDDEITIKRPAGDLTVTIVAISSSRPG
CCHHHHHHCCCCCCCEEEECCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]