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The map label for this gene is yoaE [H]

Identifier: 197117108

GI number: 197117108

Start: 845295

End: 847301

Strand: Reverse

Name: yoaE [H]

Synonym: Gbem_0713

Alternate gene names: 197117108

Gene position: 847301-845295 (Counterclockwise)

Preceding gene: 197117111

Following gene: 197117107

Centisome position: 18.36

GC content: 67.21

Gene sequence:

>2007_bases
ATGAGCGTCATTACCAAGAGATCGGTCTGCCCCTTCGACTGCCCGGACACCTGCGGCATGCTGGTGGAGGTGGAGGGGGG
AAAGGCGGTAAGCGTGAAGGGAGACCCGCTGCACCCCTTCAGCCGCGGCACCCTCTGCCCGAAGATGCGCCACTACGAGG
AAAGCGTGCACTCCCCCCTGAGGCTCACCACCCCGCTCAAGAGAACCGGCGCCAAGGGAAGCGGCGAATTCGCCCCGATC
TCCTGGGAGGAGGCGGTCGCGACCATCGCTTCTCGCTGGCGCGCCATCATCGCCGAGCACGGGGCCGAGGCGATCCTCCC
CTACTCCTATGCGGGTACCATGGGGATCGTGCAGAGAAACGCCGGGCACCCCTTCTTCCACCGGCTTGGGGCCTCCCGGC
TGGACCGCACCATCTGCTCCCCGGCCAAGGGGGCGGGGTGGCAGGCGGTGATGGGGCAGACGGCGACACCTCCCCCTGAG
ACAGCCGGCAAAAGCGACCTCCTGATCCTGTGGGGGATCAACGCGGCCGCCACCAGCATCCATTTCCTGAATCAGGCGAA
GGAGGTGCAGGCAAAAGGGGGCACGGTGTGGCTGATCGACACCTACGAGACACCGACCGCCTCGGCCGCGGACCGCACCT
TCCTGGTCCGCCCGGGAAGCGACGGGGCCCTGGCCTTGGGGATCATGCACATCCTCGCCCGGGAGGGGCTCACCGATATG
GCGTTTCTCGACTCGCAGGTGCTGGGTTATGAGGAGCTTAAGCGCGAGGTGCTCCCGCTCTACCCGCCGGCGGTATGCTC
CCGCATCACCGGTCTTGCCGTTGAAGAAATCGAACTCATGGCCCGCTTCTTTGCTTCCGCCCGCGCCCCCTTCATACGCC
TGGGTAGCGCACTGTCGCGCTACGGCAACGGCGCCATGACCGTGCGCACCATCTGCTGCCTCCCGGCTCTTGTCGGTGCC
TACGGCAAGGAAGGAGCGGGATGCTTCCCCGACACCGCCACCGGCGCCGCCTTTCCCATGGGGCTCTTGCTTCGGGAGGA
CCTGATCCAGGGCTCGCCGCGCCTGATCAACATGAACCAGCTGGGGCAGGCGCTGAACGAGCTCTCGGACCCGCCGGTCA
TGGGGCTCTACGTCTACCACTCCAACCCCGCAGCGGTCACCCCGGACCAGAACGCGGTCCTGAAGGGGCTCGCGCGGGAG
AACCTCTTCACCGTGGTGCACGAGCGCTTCATGACCGACACGGCGCGCTACGCCGATATCGTGCTTCCGGCCACCTCTTC
CCTTGAGCACAGCGACATCTACCGCTCCTACGGCACCTACTGCATACAGAGGGCGCAGGGGGCGATTGATCCCGTCGGGG
AGAGCAAGTCCAACTGGGAGGTCTTCTCGCTCCTCGCGGCAGAGCTCGGTTTCCAGGAGGAGCTCTTCAACCTCTCCGCG
GACCAGGTGATCGACCGGCTCTTAGCCGTCCCTACGCCGCTGCGCCAGGGGATCGACGAGGAGGCGCTCGCCGCGGGGCT
CCCGGTGGAACTCGCCGCGCGGGAAGGGGGATACCGGACCACCTCGGGGAAGATAGAGATCCTGAACCGGCAGCTGGCGC
ACCCGCTCCCCGTTTACCTTCCCACCCACGAGGAGAACGGGACCCTCCCCTTCAGGCTCATGACCGCCCCTAACCCCTAC
GCCCTGAACGCCACCTTCTACGAGCGGGAGGAACTAAGGGCGCGGCAGGGGGGGATGCAACTCAGGATGAACCCCGCCGA
CGCCGCGGCCAAGGGGCTTGCCGACGGCGAGCGGGTGGTGGCCTGGAACGCGCTCGGAGAGGTGACCTTCCTGCTCAAGA
CGACGGACAAGGTCCCCGCCTGCCTCGTGGTCGCGGAGGGGGTGTGGTGGCTCGCCTACGCGCCGGGGAGCCGCTCGGTG
AACGCCCTCACCTCGCAGCGCCTGACCGACGAGGGCGGAGGAAGCACCTTCTACGACAACCGCGTCGACGTGCGCCGGGA
GCTCTAA

Upstream 100 bases:

>100_bases
CTCCCGTCAAGGGAGGGGGGGGTAAACCCCTAAAAACCTTCATTCGAGAAGACCAGGCGACCGCTTACCGCCGGTTTTTG
CCGTGCAAAGGAGATGCAAG

Downstream 100 bases:

>100_bases
TGGAGATTTTTGCCTCATGCTTTTAATCATTGTGCCGATGTAGCAAGGAGAAAGCGGCAAAAAAAGCGATGCGGGGGGCA
CATGGAACCGAGCAACAAGA

Product: molybdopterin-binding iron-sulfur cluster-binding oxidoreductase MopB-3

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MSVITKRSVCPFDCPDTCGMLVEVEGGKAVSVKGDPLHPFSRGTLCPKMRHYEESVHSPLRLTTPLKRTGAKGSGEFAPI
SWEEAVATIASRWRAIIAEHGAEAILPYSYAGTMGIVQRNAGHPFFHRLGASRLDRTICSPAKGAGWQAVMGQTATPPPE
TAGKSDLLILWGINAAATSIHFLNQAKEVQAKGGTVWLIDTYETPTASAADRTFLVRPGSDGALALGIMHILAREGLTDM
AFLDSQVLGYEELKREVLPLYPPAVCSRITGLAVEEIELMARFFASARAPFIRLGSALSRYGNGAMTVRTICCLPALVGA
YGKEGAGCFPDTATGAAFPMGLLLREDLIQGSPRLINMNQLGQALNELSDPPVMGLYVYHSNPAAVTPDQNAVLKGLARE
NLFTVVHERFMTDTARYADIVLPATSSLEHSDIYRSYGTYCIQRAQGAIDPVGESKSNWEVFSLLAAELGFQEELFNLSA
DQVIDRLLAVPTPLRQGIDEEALAAGLPVELAAREGGYRTTSGKIEILNRQLAHPLPVYLPTHEENGTLPFRLMTAPNPY
ALNATFYEREELRARQGGMQLRMNPADAAAKGLADGERVVAWNALGEVTFLLKTTDKVPACLVVAEGVWWLAYAPGSRSV
NALTSQRLTDEGGGSTFYDNRVDVRREL

Sequences:

>Translated_668_residues
MSVITKRSVCPFDCPDTCGMLVEVEGGKAVSVKGDPLHPFSRGTLCPKMRHYEESVHSPLRLTTPLKRTGAKGSGEFAPI
SWEEAVATIASRWRAIIAEHGAEAILPYSYAGTMGIVQRNAGHPFFHRLGASRLDRTICSPAKGAGWQAVMGQTATPPPE
TAGKSDLLILWGINAAATSIHFLNQAKEVQAKGGTVWLIDTYETPTASAADRTFLVRPGSDGALALGIMHILAREGLTDM
AFLDSQVLGYEELKREVLPLYPPAVCSRITGLAVEEIELMARFFASARAPFIRLGSALSRYGNGAMTVRTICCLPALVGA
YGKEGAGCFPDTATGAAFPMGLLLREDLIQGSPRLINMNQLGQALNELSDPPVMGLYVYHSNPAAVTPDQNAVLKGLARE
NLFTVVHERFMTDTARYADIVLPATSSLEHSDIYRSYGTYCIQRAQGAIDPVGESKSNWEVFSLLAAELGFQEELFNLSA
DQVIDRLLAVPTPLRQGIDEEALAAGLPVELAAREGGYRTTSGKIEILNRQLAHPLPVYLPTHEENGTLPFRLMTAPNPY
ALNATFYEREELRARQGGMQLRMNPADAAAKGLADGERVVAWNALGEVTFLLKTTDKVPACLVVAEGVWWLAYAPGSRSV
NALTSQRLTDEGGGSTFYDNRVDVRREL
>Mature_667_residues
SVITKRSVCPFDCPDTCGMLVEVEGGKAVSVKGDPLHPFSRGTLCPKMRHYEESVHSPLRLTTPLKRTGAKGSGEFAPIS
WEEAVATIASRWRAIIAEHGAEAILPYSYAGTMGIVQRNAGHPFFHRLGASRLDRTICSPAKGAGWQAVMGQTATPPPET
AGKSDLLILWGINAAATSIHFLNQAKEVQAKGGTVWLIDTYETPTASAADRTFLVRPGSDGALALGIMHILAREGLTDMA
FLDSQVLGYEELKREVLPLYPPAVCSRITGLAVEEIELMARFFASARAPFIRLGSALSRYGNGAMTVRTICCLPALVGAY
GKEGAGCFPDTATGAAFPMGLLLREDLIQGSPRLINMNQLGQALNELSDPPVMGLYVYHSNPAAVTPDQNAVLKGLAREN
LFTVVHERFMTDTARYADIVLPATSSLEHSDIYRSYGTYCIQRAQGAIDPVGESKSNWEVFSLLAAELGFQEELFNLSAD
QVIDRLLAVPTPLRQGIDEEALAAGLPVELAAREGGYRTTSGKIEILNRQLAHPLPVYLPTHEENGTLPFRLMTAPNPYA
LNATFYEREELRARQGGMQLRMNPADAAAKGLADGERVVAWNALGEVTFLLKTTDKVPACLVVAEGVWWLAYAPGSRSVN
ALTSQRLTDEGGGSTFYDNRVDVRREL

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=765, Percent_Identity=27.1895424836601, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1787870, Length=767, Percent_Identity=26.4667535853977, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI171474008, Length=755, Percent_Identity=25.1655629139073, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI3868721, Length=696, Percent_Identity=25.1436781609195, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI87081994, Length=717, Percent_Identity=23.9888423988842, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1788534, Length=530, Percent_Identity=22.2641509433962, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 72319; Mature: 72188

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVITKRSVCPFDCPDTCGMLVEVEGGKAVSVKGDPLHPFSRGTLCPKMRHYEESVHSPL
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCE
RLTTPLKRTGAKGSGEFAPISWEEAVATIASRWRAIIAEHGAEAILPYSYAGTMGIVQRN
EEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHEECC
AGHPFFHRLGASRLDRTICSPAKGAGWQAVMGQTATPPPETAGKSDLLILWGINAAATSI
CCCHHHHHHCHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHH
HFLNQAKEVQAKGGTVWLIDTYETPTASAADRTFLVRPGSDGALALGIMHILAREGLTDM
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHH
AFLDSQVLGYEELKREVLPLYPPAVCSRITGLAVEEIELMARFFASARAPFIRLGSALSR
HHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
YGNGAMTVRTICCLPALVGAYGKEGAGCFPDTATGAAFPMGLLLREDLIQGSPRLINMNQ
HCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHH
LGQALNELSDPPVMGLYVYHSNPAAVTPDQNAVLKGLARENLFTVVHERFMTDTARYADI
HHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VLPATSSLEHSDIYRSYGTYCIQRAQGAIDPVGESKSNWEVFSLLAAELGFQEELFNLSA
EECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCH
DQVIDRLLAVPTPLRQGIDEEALAAGLPVELAAREGGYRTTSGKIEILNRQLAHPLPVYL
HHHHHHHHHCCCHHHHCCCHHHHHCCCCEEEEECCCCCCCCCCHHEEHHHHHCCCCCEEE
PTHEENGTLPFRLMTAPNPYALNATFYEREELRARQGGMQLRMNPADAAAKGLADGERVV
CCCCCCCCCCEEEEECCCCEEECCCHHHHHHHHHHHCCCEEEECHHHHHHHCCCCCCEEE
AWNALGEVTFLLKTTDKVPACLVVAEGVWWLAYAPGSRSVNALTSQRLTDEGGGSTFYDN
EEECCCCEEEEEEECCCCCEEEEEECCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCC
RVDVRREL
CCHHHCCC
>Mature Secondary Structure 
SVITKRSVCPFDCPDTCGMLVEVEGGKAVSVKGDPLHPFSRGTLCPKMRHYEESVHSPL
CCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCE
RLTTPLKRTGAKGSGEFAPISWEEAVATIASRWRAIIAEHGAEAILPYSYAGTMGIVQRN
EEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHEECC
AGHPFFHRLGASRLDRTICSPAKGAGWQAVMGQTATPPPETAGKSDLLILWGINAAATSI
CCCHHHHHHCHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHH
HFLNQAKEVQAKGGTVWLIDTYETPTASAADRTFLVRPGSDGALALGIMHILAREGLTDM
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHH
AFLDSQVLGYEELKREVLPLYPPAVCSRITGLAVEEIELMARFFASARAPFIRLGSALSR
HHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
YGNGAMTVRTICCLPALVGAYGKEGAGCFPDTATGAAFPMGLLLREDLIQGSPRLINMNQ
HCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHH
LGQALNELSDPPVMGLYVYHSNPAAVTPDQNAVLKGLARENLFTVVHERFMTDTARYADI
HHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VLPATSSLEHSDIYRSYGTYCIQRAQGAIDPVGESKSNWEVFSLLAAELGFQEELFNLSA
EECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCH
DQVIDRLLAVPTPLRQGIDEEALAAGLPVELAAREGGYRTTSGKIEILNRQLAHPLPVYL
HHHHHHHHHCCCHHHHCCCHHHHHCCCCEEEEECCCCCCCCCCHHEEHHHHHCCCCCEEE
PTHEENGTLPFRLMTAPNPYALNATFYEREELRARQGGMQLRMNPADAAAKGLADGERVV
CCCCCCCCCCEEEEECCCCEEECCCHHHHHHHHHHHCCCEEEECHHHHHHHCCCCCCEEE
AWNALGEVTFLLKTTDKVPACLVVAEGVWWLAYAPGSRSVNALTSQRLTDEGGGSTFYDN
EEECCCCEEEEEEECCCCCEEEEEECCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCC
RVDVRREL
CCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]