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The map label for this gene is sucB [H]

Identifier: 197117101

GI number: 197117101

Start: 835431

End: 836702

Strand: Direct

Name: sucB [H]

Synonym: Gbem_0705

Alternate gene names: 197117101

Gene position: 835431-836702 (Clockwise)

Preceding gene: 308535183

Following gene: 308535184

Centisome position: 18.1

GC content: 67.14

Gene sequence:

>1272_bases
ATGGATATTAAGGTTCCCGCGGTGGGTGAGTCGGTCTATGAGGCGGTCATCGCCAGGTGGCTCAAGAAGTCGGGCGAGGT
TGTGGCCAAGGATGAGCCGCTTTGCGAGGTTGAAACGGACAAGGTGACCCTGGAGGTGACCTCGGAGGCGGACGGCGTGT
TGACCACGCTGGCGGCCGAGGGGGAGACGGTGAAGATCGGCGCGGTGATCGCGACCATAGACGCCCGCGGCGCAGAGGCC
GCGCCCCCCTCGGGGAGCGCCGCCCCTCCTGAGCCCCCGCCTTCCGGCGCCCCGGCAGCCGGGACCGCCCCGGCCAAAGC
GACCCCCAAGGGTATGGCGCCTCCCATCTCCCCATCGGGGCGCAAGCTGGCACGCGAGCTGGGCGTGGAGCCCCAGGATG
TCAAGGGGACCGGGCGCGGAGGGCGCGTGACCAGGGAAGACCTTTTGAAGGCGGAGGGGGCAGAGCCCGCTCCCGCCGAG
GCCGAACCCTCCCCTGCGGCACAGCTTGCGCCGGTCGGGAAAACCGAGCCGGCACCCGCTGCTCCGCCCCGTCCCGCCCC
AGCCGAAGAAGCGGAGCGCGTGGTCCGCAAGCCGATGACGCAGATCAGAAAGCGCATTGCGGAGCGGCTGGTCTCGGTGC
GGCAGCAGACCGCCATGCTCACCACCTTCAACGAGGCGGACATGAGCCAGGTGATGCTGCTGCGCCAGAAGTACGGGGAA
CATTTCCTGAAGCGCCACGGCGTGAAGCTGGGGTTCATGTCCTTTTTCGTCCGCGCCTGCTGCGAGGCGCTGGCGCAGTA
TCCCGAGGTGAACGCGCAGATCGATGGCGACGACATCGTCTACCACAACTTCTGTGACATCGGCGTTGCCGTCGGGAGCG
AAAGGGGGCTGGTGGTACCGGTCTTGAGAGGTGCCGAGTCGCTCACCCTCGCCGGGATCGAGCAGCAGATCGCACAATAC
GCCGAGAAGGTGCGCCAAAACCGCATCGCCATCGCCGACCTTGAGGGGGGGACCTTCACCATCTCCAACGGCGGGGTCTA
CGGTTCCATGCTCTCCACCCCCATCCTCAACCCGCCGCAAAGCGGGGTGCTGGGGATGCACGCGATACAGCCGCGCGCCG
TGGTCGTCGACGGCGAGATCGTGGTCCGCCCCATGATGTACCTGGCGCTCTCCTACGATCACCGCATCGTGGACGGGCAG
GGGGCGGTGGGGTTTTTGAAGCTGGTCAAGGAGTACATCGAGGAGCCGGAGGAGTTGCTGCTCGAAGGGTAG

Upstream 100 bases:

>100_bases
ACGCTTTTGCCTAGCGCTTAACTCAGTAACTAAGAAGCACCGGTCCCGGTGAAGGTCAAAGCCGTGAACCTGCACCTTTG
GCCGGTTTAGGAGTCGGAAA

Downstream 100 bases:

>100_bases
CTACAACGCACACCGCTGCCCGCGTGGCAGGGGGAGTAGTAGGGAGGCGAGATGGCAGAAGAAATTTTCGATCTGGTGGT
TATCGGGGCGGGGCCCGGCG

Product: 2-oxoglutarate dehydrogenase, E2 protein, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MDIKVPAVGESVYEAVIARWLKKSGEVVAKDEPLCEVETDKVTLEVTSEADGVLTTLAAEGETVKIGAVIATIDARGAEA
APPSGSAAPPEPPPSGAPAAGTAPAKATPKGMAPPISPSGRKLARELGVEPQDVKGTGRGGRVTREDLLKAEGAEPAPAE
AEPSPAAQLAPVGKTEPAPAAPPRPAPAEEAERVVRKPMTQIRKRIAERLVSVRQQTAMLTTFNEADMSQVMLLRQKYGE
HFLKRHGVKLGFMSFFVRACCEALAQYPEVNAQIDGDDIVYHNFCDIGVAVGSERGLVVPVLRGAESLTLAGIEQQIAQY
AEKVRQNRIAIADLEGGTFTISNGGVYGSMLSTPILNPPQSGVLGMHAIQPRAVVVDGEIVVRPMMYLALSYDHRIVDGQ
GAVGFLKLVKEYIEEPEELLLEG

Sequences:

>Translated_423_residues
MDIKVPAVGESVYEAVIARWLKKSGEVVAKDEPLCEVETDKVTLEVTSEADGVLTTLAAEGETVKIGAVIATIDARGAEA
APPSGSAAPPEPPPSGAPAAGTAPAKATPKGMAPPISPSGRKLARELGVEPQDVKGTGRGGRVTREDLLKAEGAEPAPAE
AEPSPAAQLAPVGKTEPAPAAPPRPAPAEEAERVVRKPMTQIRKRIAERLVSVRQQTAMLTTFNEADMSQVMLLRQKYGE
HFLKRHGVKLGFMSFFVRACCEALAQYPEVNAQIDGDDIVYHNFCDIGVAVGSERGLVVPVLRGAESLTLAGIEQQIAQY
AEKVRQNRIAIADLEGGTFTISNGGVYGSMLSTPILNPPQSGVLGMHAIQPRAVVVDGEIVVRPMMYLALSYDHRIVDGQ
GAVGFLKLVKEYIEEPEELLLEG
>Mature_423_residues
MDIKVPAVGESVYEAVIARWLKKSGEVVAKDEPLCEVETDKVTLEVTSEADGVLTTLAAEGETVKIGAVIATIDARGAEA
APPSGSAAPPEPPPSGAPAAGTAPAKATPKGMAPPISPSGRKLARELGVEPQDVKGTGRGGRVTREDLLKAEGAEPAPAE
AEPSPAAQLAPVGKTEPAPAAPPRPAPAEEAERVVRKPMTQIRKRIAERLVSVRQQTAMLTTFNEADMSQVMLLRQKYGE
HFLKRHGVKLGFMSFFVRACCEALAQYPEVNAQIDGDDIVYHNFCDIGVAVGSERGLVVPVLRGAESLTLAGIEQQIAQY
AEKVRQNRIAIADLEGGTFTISNGGVYGSMLSTPILNPPQSGVLGMHAIQPRAVVVDGEIVVRPMMYLALSYDHRIVDGQ
GAVGFLKLVKEYIEEPEELLLEG

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=229, Percent_Identity=53.2751091703057, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI110671329, Length=431, Percent_Identity=27.3781902552204, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI31711992, Length=447, Percent_Identity=28.1879194630872, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=26.7108167770419, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=26.7108167770419, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=30.4347826086957, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1786946, Length=423, Percent_Identity=46.8085106382979, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1786305, Length=433, Percent_Identity=28.6374133949192, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=429, Percent_Identity=40.0932400932401, Blast_Score=272, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17537937, Length=432, Percent_Identity=27.0833333333333, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=453, Percent_Identity=28.476821192053, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=26.7100977198697, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=421, Percent_Identity=41.3301662707838, Blast_Score=297, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6324258, Length=453, Percent_Identity=26.4900662251656, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24645909, Length=245, Percent_Identity=49.7959183673469, Blast_Score=251, Evalue=8e-67,
Organism=Drosophila melanogaster, GI18859875, Length=429, Percent_Identity=31.2354312354312, Blast_Score=164, Evalue=9e-41,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=26.7543859649123, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=26.7543859649123, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44814; Mature: 44814

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKVPAVGESVYEAVIARWLKKSGEVVAKDEPLCEVETDKVTLEVTSEADGVLTTLAAE
CCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCEEEECCEEEEEECCCCCCEEEEEECC
GETVKIGAVIATIDARGAEAAPPSGSAAPPEPPPSGAPAAGTAPAKATPKGMAPPISPSG
CCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
RKLARELGVEPQDVKGTGRGGRVTREDLLKAEGAEPAPAEAEPSPAAQLAPVGKTEPAPA
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCC
APPRPAPAEEAERVVRKPMTQIRKRIAERLVSVRQQTAMLTTFNEADMSQVMLLRQKYGE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHH
HFLKRHGVKLGFMSFFVRACCEALAQYPEVNAQIDGDDIVYHNFCDIGVAVGSERGLVVP
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEECHHHHHEEECCCCCEEEE
VLRGAESLTLAGIEQQIAQYAEKVRQNRIAIADLEGGTFTISNGGVYGSMLSTPILNPPQ
EECCCCCEEHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEECCCEEHHHHCCCCCCCCC
SGVLGMHAIQPRAVVVDGEIVVRPMMYLALSYDHRIVDGQGAVGFLKLVKEYIEEPEELL
CCCEEEEECCCEEEEECCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCHHHHH
LEG
CCC
>Mature Secondary Structure
MDIKVPAVGESVYEAVIARWLKKSGEVVAKDEPLCEVETDKVTLEVTSEADGVLTTLAAE
CCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCEEEECCEEEEEECCCCCCEEEEEECC
GETVKIGAVIATIDARGAEAAPPSGSAAPPEPPPSGAPAAGTAPAKATPKGMAPPISPSG
CCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
RKLARELGVEPQDVKGTGRGGRVTREDLLKAEGAEPAPAEAEPSPAAQLAPVGKTEPAPA
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCC
APPRPAPAEEAERVVRKPMTQIRKRIAERLVSVRQQTAMLTTFNEADMSQVMLLRQKYGE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHH
HFLKRHGVKLGFMSFFVRACCEALAQYPEVNAQIDGDDIVYHNFCDIGVAVGSERGLVVP
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEECHHHHHEEECCCCCEEEE
VLRGAESLTLAGIEQQIAQYAEKVRQNRIAIADLEGGTFTISNGGVYGSMLSTPILNPPQ
EECCCCCEEHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEECCCEEHHHHCCCCCCCCC
SGVLGMHAIQPRAVVVDGEIVVRPMMYLALSYDHRIVDGQGAVGFLKLVKEYIEEPEELL
CCCEEEEECCCEEEEECCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCHHHHH
LEG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA