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The map label for this gene is def-1 [H]

Identifier: 197117031

GI number: 197117031

Start: 754441

End: 754959

Strand: Direct

Name: def-1 [H]

Synonym: Gbem_0634

Alternate gene names: 197117031

Gene position: 754441-754959 (Clockwise)

Preceding gene: 197117029

Following gene: 197117032

Centisome position: 16.35

GC content: 62.04

Gene sequence:

>519_bases
ATGATACGAACAATACTCACCTATCCGGACCCTGAGCTGAAGAAGCGCTCACTGCCGGTTACCGTTATCACCGACAAGAC
CCGCGAGTTGGCTCGCGACATGGCCGAGACCATGTACGACGCCCCCGGAGTGGGGCTTGCCGCCCCCCAGATAGGGGTGC
ACCAGCGCATCATCGTCATCGACGTCTCCGGCAAGGACGAGACGCCCGAGCTGATCGTGGCCATCAACCCCGAGATCGTC
CACGCCGAGGGGGAGGCGTTCGAGGAGGAAGGGTGCCTGTCGGTCCCCAAGTTCTCCGCCAACGTGCGCCGGCACGCCCG
CATCGTGGTGAAGGCGCTCAACCTGGAGGGCGAGGAAGTGACCTTCCGCGCCGACGACCTCTTGTCCATCGCTTTCCAAC
ACGAGATCGACCACCTGGACGGCGTGCTCTTCATCGACCATCTTTCCCCGCTCAAGAAAGGGATCTTCCGCAAGCGATAC
CAGCGCGCCTTGGACGAAGCAAAGGAGCTTAACCGATGA

Upstream 100 bases:

>100_bases
CCCCTCTGAAGGGGGACGTTGCTCATGAGTATCTCGTTGATTTTTTGCCGGCAACTTTATATAGTGGCGCGATAGTTCTG
AAGACAAAGGAAGGACCAGT

Downstream 100 bases:

>100_bases
CCGGTATGCGCATCGTCTTCATGGGGACCCCCGAATTCGCCTGCCCCACCTTGCGCACGCTGATCGAGCGCGGCGAAAAG
GTGGTGGCCGTGGTCACCCA

Product: polypeptide formylmethionine deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MIRTILTYPDPELKKRSLPVTVITDKTRELARDMAETMYDAPGVGLAAPQIGVHQRIIVIDVSGKDETPELIVAINPEIV
HAEGEAFEEEGCLSVPKFSANVRRHARIVVKALNLEGEEVTFRADDLLSIAFQHEIDHLDGVLFIDHLSPLKKGIFRKRY
QRALDEAKELNR

Sequences:

>Translated_172_residues
MIRTILTYPDPELKKRSLPVTVITDKTRELARDMAETMYDAPGVGLAAPQIGVHQRIIVIDVSGKDETPELIVAINPEIV
HAEGEAFEEEGCLSVPKFSANVRRHARIVVKALNLEGEEVTFRADDLLSIAFQHEIDHLDGVLFIDHLSPLKKGIFRKRY
QRALDEAKELNR
>Mature_172_residues
MIRTILTYPDPELKKRSLPVTVITDKTRELARDMAETMYDAPGVGLAAPQIGVHQRIIVIDVSGKDETPELIVAINPEIV
HAEGEAFEEEGCLSVPKFSANVRRHARIVVKALNLEGEEVTFRADDLLSIAFQHEIDHLDGVLFIDHLSPLKKGIFRKRY
QRALDEAKELNR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=45.1219512195122, Blast_Score=135, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=30.5732484076433, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=29.9363057324841, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19362; Mature: 19362

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTILTYPDPELKKRSLPVTVITDKTRELARDMAETMYDAPGVGLAAPQIGVHQRIIVI
CCEEEEECCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
DVSGKDETPELIVAINPEIVHAEGEAFEEEGCLSVPKFSANVRRHARIVVKALNLEGEEV
EECCCCCCCCEEEEECCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHEEEHEECCCCCEE
TFRADDLLSIAFQHEIDHLDGVLFIDHLSPLKKGIFRKRYQRALDEAKELNR
EEEHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRTILTYPDPELKKRSLPVTVITDKTRELARDMAETMYDAPGVGLAAPQIGVHQRIIVI
CCEEEEECCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
DVSGKDETPELIVAINPEIVHAEGEAFEEEGCLSVPKFSANVRRHARIVVKALNLEGEEV
EECCCCCCCCEEEEECCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHEEEHEECCCCCEE
TFRADDLLSIAFQHEIDHLDGVLFIDHLSPLKKGIFRKRYQRALDEAKELNR
EEEHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA