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The map label for this gene is fmt

Identifier: 197117032

GI number: 197117032

Start: 754956

End: 755912

Strand: Direct

Name: fmt

Synonym: Gbem_0635

Alternate gene names: 197117032

Gene position: 754956-755912 (Clockwise)

Preceding gene: 197117031

Following gene: 308535179

Centisome position: 16.36

GC content: 66.04

Gene sequence:

>957_bases
ATGACCGGTATGCGCATCGTCTTCATGGGGACCCCCGAATTCGCCTGCCCCACCTTGCGCACGCTGATCGAGCGCGGCGA
AAAGGTGGTGGCCGTGGTCACCCAGCCCGACCGCCCCAAGGGGCGCGGGCAGCAGACCCTCCCCCCGCCGGTGAAGGTCG
TCGCCGAGCAGCACGGGATACCGGTGCTGCAGCCGGTTAAGGTGCGGCTTCCCGAGTCGATCGAAGAGATCCGGGGACTT
AACCCCGACCTCATCGTGGTGATAGCTTTCGGGCAGATCCTGCCCAAGGCGCTGCTCGACATCCCCAAGTACGGCTGCAT
CAACGTGCACGCCTCGCTTTTGCCGCGCTACCGCGGCGCCGCGCCCTTGAACTGGTGCATCATCAACGGCGAGAACGAGA
CCGGGGTCACCACCATGATGATGGACGTGGGGCTCGATACCGGCGACATGCTCCTGAAGCGCTCCACCCCCATCGGCGCC
GACGAAGATACCCAGAGCCTGCACGACCGGATGTCGCAGCTTGGGGCGGAGCTCCTCGCCGAGACGCTGGACCGGCTGGC
CCGGGGCGAACTGGTCCCGGAGAAGCAGGACGACGCGCTCACCTGCTACGCCCCGATGATGAAAAAAGAGGACGGTCTCA
TCGACTGGAGCCGGGATGCGCAGGCCATCAAGAACCAGGTCCGCGGCATGACCCCCTGGCCGGGCGCCTACAGCTTCCTG
GACGACAAGCTTTTGAAGGTCTTCCGCGTTCAGACCGCTTCCGGGAGCGGCGCGCCGGGCGAGATCCTCTCCTGCGGCCG
TGACGGCATCGAGGTCGCCTGCGGCACGGGTAGCCTGGTGATCGCGGAACTGCAGCTCGAAGGCAAGAAGCGCCTGCCGG
CGGGGGACTTCCTCGCAGGGTACAAACTCCAGCCGGGCGGCCTGCTCGGAAAGAAGGATGCTTCCGTTGGGGTATAA

Upstream 100 bases:

>100_bases
TGGACGGCGTGCTCTTCATCGACCATCTTTCCCCGCTCAAGAAAGGGATCTTCCGCAAGCGATACCAGCGCGCCTTGGAC
GAAGCAAAGGAGCTTAACCG

Downstream 100 bases:

>100_bases
GGGGCCATATCAGGGGCCTCCCCGGAAGCGCCTCTTCGTGGCACTGATGGGGGTGACCTGCCTCCTGGTGGTGGGGCTCA
TCTACCTTGGGTGGTGGATC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTGMRIVFMGTPEFACPTLRTLIERGEKVVAVVTQPDRPKGRGQQTLPPPVKVVAEQHGIPVLQPVKVRLPESIEEIRGL
NPDLIVVIAFGQILPKALLDIPKYGCINVHASLLPRYRGAAPLNWCIINGENETGVTTMMMDVGLDTGDMLLKRSTPIGA
DEDTQSLHDRMSQLGAELLAETLDRLARGELVPEKQDDALTCYAPMMKKEDGLIDWSRDAQAIKNQVRGMTPWPGAYSFL
DDKLLKVFRVQTASGSGAPGEILSCGRDGIEVACGTGSLVIAELQLEGKKRLPAGDFLAGYKLQPGGLLGKKDASVGV

Sequences:

>Translated_318_residues
MTGMRIVFMGTPEFACPTLRTLIERGEKVVAVVTQPDRPKGRGQQTLPPPVKVVAEQHGIPVLQPVKVRLPESIEEIRGL
NPDLIVVIAFGQILPKALLDIPKYGCINVHASLLPRYRGAAPLNWCIINGENETGVTTMMMDVGLDTGDMLLKRSTPIGA
DEDTQSLHDRMSQLGAELLAETLDRLARGELVPEKQDDALTCYAPMMKKEDGLIDWSRDAQAIKNQVRGMTPWPGAYSFL
DDKLLKVFRVQTASGSGAPGEILSCGRDGIEVACGTGSLVIAELQLEGKKRLPAGDFLAGYKLQPGGLLGKKDASVGV
>Mature_317_residues
TGMRIVFMGTPEFACPTLRTLIERGEKVVAVVTQPDRPKGRGQQTLPPPVKVVAEQHGIPVLQPVKVRLPESIEEIRGLN
PDLIVVIAFGQILPKALLDIPKYGCINVHASLLPRYRGAAPLNWCIINGENETGVTTMMMDVGLDTGDMLLKRSTPIGAD
EDTQSLHDRMSQLGAELLAETLDRLARGELVPEKQDDALTCYAPMMKKEDGLIDWSRDAQAIKNQVRGMTPWPGAYSFLD
DKLLKVFRVQTASGSGAPGEILSCGRDGIEVACGTGSLVIAELQLEGKKRLPAGDFLAGYKLQPGGLLGKKDASVGV

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=314, Percent_Identity=28.343949044586, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI21614513, Length=315, Percent_Identity=29.5238095238095, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI164663775, Length=323, Percent_Identity=30.030959752322, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1789683, Length=299, Percent_Identity=47.4916387959866, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI1788589, Length=283, Percent_Identity=31.095406360424, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI133930964, Length=259, Percent_Identity=28.957528957529, Blast_Score=118, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6319458, Length=363, Percent_Identity=26.4462809917355, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45550868, Length=312, Percent_Identity=30.4487179487179, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28571984, Length=234, Percent_Identity=32.9059829059829, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24585660, Length=241, Percent_Identity=31.1203319502075, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_GEOBB (B5ED77)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137459.1
- ProteinModelPortal:   B5ED77
- SMR:   B5ED77
- GeneID:   6783468
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0635
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   CLSK827657
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34347; Mature: 34216

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGMRIVFMGTPEFACPTLRTLIERGEKVVAVVTQPDRPKGRGQQTLPPPVKVVAEQHGI
CCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCC
PVLQPVKVRLPESIEEIRGLNPDLIVVIAFGQILPKALLDIPKYGCINVHASLLPRYRGA
CCCCCHHCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCC
APLNWCIINGENETGVTTMMMDVGLDTGDMLLKRSTPIGADEDTQSLHDRMSQLGAELLA
CCEEEEEEECCCCCCCEEEEEECCCCCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHH
ETLDRLARGELVPEKQDDALTCYAPMMKKEDGLIDWSRDAQAIKNQVRGMTPWPGAYSFL
HHHHHHHCCCCCCCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
DDKLLKVFRVQTASGSGAPGEILSCGRDGIEVACGTGSLVIAELQLEGKKRLPAGDFLAG
HHHHHHHHHEECCCCCCCCHHHHHCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCC
YKLQPGGLLGKKDASVGV
CEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGMRIVFMGTPEFACPTLRTLIERGEKVVAVVTQPDRPKGRGQQTLPPPVKVVAEQHGI
CCCEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCC
PVLQPVKVRLPESIEEIRGLNPDLIVVIAFGQILPKALLDIPKYGCINVHASLLPRYRGA
CCCCCHHCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCC
APLNWCIINGENETGVTTMMMDVGLDTGDMLLKRSTPIGADEDTQSLHDRMSQLGAELLA
CCEEEEEEECCCCCCCEEEEEECCCCCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHH
ETLDRLARGELVPEKQDDALTCYAPMMKKEDGLIDWSRDAQAIKNQVRGMTPWPGAYSFL
HHHHHHHCCCCCCCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
DDKLLKVFRVQTASGSGAPGEILSCGRDGIEVACGTGSLVIAELQLEGKKRLPAGDFLAG
HHHHHHHHHEECCCCCCCCHHHHHCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCC
YKLQPGGLLGKKDASVGV
CEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA