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The map label for this gene is gspF [H]

Identifier: 197116990

GI number: 197116990

Start: 710981

End: 712183

Strand: Reverse

Name: gspF [H]

Synonym: Gbem_0593

Alternate gene names: 197116990

Gene position: 712183-710981 (Counterclockwise)

Preceding gene: 197116991

Following gene: 197116989

Centisome position: 15.43

GC content: 64.17

Gene sequence:

>1203_bases
ATGCCGACCTTTCGCTATAGCGCCTTCAACCAGAAAGGGACCGAGGTCTCCGGGACCGTGGATGCGGCCGGCGAAACCGA
GGCGCGGCTGCAACTGAAGCAGAAGGGGCTCTACGCCAAGGAGATCGCCCCCGCCGTCGAAGGTGGGAAGCGGAAGCTCT
TCAGCTCCGGCGTCAGCGTTCCCGACCTCTCGCTCGCAACCCGAAGGCTGGCGACCCTACTTGGTAGCGCCGTCCCGGTT
TACGAGGCGGTGGCGACGCTTTGGGAGCAGGAAGCGCCGGGCGAACTGAAAAAGGTATTAGGGCGAGTGCGGGACCGGCT
TGCCGAGGGGCAGGGGCTGGCGCAATCGATGGCCGCCGAGCCAGCCGTTTTCTCCGAGAGCTACATCGGCATGGTCGCGG
CCGGCGAGGCAAGCGGCGCGCTGGAAGTGGTGCTGGAGCGGCTGGCCGAGTTCCAGGAGGATCAGGCCGAAGTGAGGAGC
CGGGTCATCACCGCGCTCATCTACCCCGCCATCATGGTGGTGGTGGGGACGGCGGTCATGCTGGTGCTCCTGGGGTTCGT
TGTCCCCAAGATATCCGCCGTCTTCGAGACCAACAAGGCGACCCTTCCCATGGTGACCATCATCCTCATCAAGGCGAGCG
CCCTGGTGAGGAAGGGGTGGTGGCTCCTTTTGGCCGCGGCGTTCGCACTCTGGACCGGGTACAAGCGGCTGATCAAGAGC
GAGGAACTGGTGCGAAAGCGCGACCGCCTGATTTTGAAGCTCCCCGTGGCGGGGCAGCTCTGGCAGCGCCTGGTGCTGTC
GCGTTTCGCCAAGGTGCTGGGGCTGTTGCTGCAAAGCGGGGTCCCCATCATCAAGGCGATGGACATAACCGGCGAGGCGG
TGGTGAACCGGGAGTACAAGGCGTTTCTGGCCGAGGCCAGGGAGAGCCTGATGCAGGGGGGGAGCCTCTCGGCGTCGCTC
AAGGCGAGTCCGCTCTTCCCGCCGCTTTTGACCCACATGACCGCGGTGGGAGAGAAGAGCGGCGAGCTGGACGGGATGCT
GATTAAGGCCGGCGACGCCTTCCAGAAGGAATTCAACGCCACCGTGACCAGGTCCATGGCGATGCTGGAGCCGCTCCTCA
TATTGGGAATGGGGCTCACCATAGGCTTCATGGTCATAGCGGTGCTGCTCCCCATCATGCAGCTCAATCAGCTGGTCAAA
TGA

Upstream 100 bases:

>100_bases
GTCTCCAAGGGGATGCGCACCCTGCGCGAGGACGGCCTCGCCAAGGCAGCCCAGGGGATCACCACCATCGAGGAGGTCCT
GAGGGTGACTCAGGACGACT

Downstream 100 bases:

>100_bases
GCAACGGGATACCATCGCTTAAATACAATCCGACTTTCTGATATTGGAGGTTTTGCAATGTTCAAGACACTGAAAGACAG
CCGCGGCTTCACACTGATCG

Product: type II secretion system protein GspF

Products: NA

Alternate protein names: Pectic enzymes secretion protein outF [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MPTFRYSAFNQKGTEVSGTVDAAGETEARLQLKQKGLYAKEIAPAVEGGKRKLFSSGVSVPDLSLATRRLATLLGSAVPV
YEAVATLWEQEAPGELKKVLGRVRDRLAEGQGLAQSMAAEPAVFSESYIGMVAAGEASGALEVVLERLAEFQEDQAEVRS
RVITALIYPAIMVVVGTAVMLVLLGFVVPKISAVFETNKATLPMVTIILIKASALVRKGWWLLLAAAFALWTGYKRLIKS
EELVRKRDRLILKLPVAGQLWQRLVLSRFAKVLGLLLQSGVPIIKAMDITGEAVVNREYKAFLAEARESLMQGGSLSASL
KASPLFPPLLTHMTAVGEKSGELDGMLIKAGDAFQKEFNATVTRSMAMLEPLLILGMGLTIGFMVIAVLLPIMQLNQLVK

Sequences:

>Translated_400_residues
MPTFRYSAFNQKGTEVSGTVDAAGETEARLQLKQKGLYAKEIAPAVEGGKRKLFSSGVSVPDLSLATRRLATLLGSAVPV
YEAVATLWEQEAPGELKKVLGRVRDRLAEGQGLAQSMAAEPAVFSESYIGMVAAGEASGALEVVLERLAEFQEDQAEVRS
RVITALIYPAIMVVVGTAVMLVLLGFVVPKISAVFETNKATLPMVTIILIKASALVRKGWWLLLAAAFALWTGYKRLIKS
EELVRKRDRLILKLPVAGQLWQRLVLSRFAKVLGLLLQSGVPIIKAMDITGEAVVNREYKAFLAEARESLMQGGSLSASL
KASPLFPPLLTHMTAVGEKSGELDGMLIKAGDAFQKEFNATVTRSMAMLEPLLILGMGLTIGFMVIAVLLPIMQLNQLVK
>Mature_399_residues
PTFRYSAFNQKGTEVSGTVDAAGETEARLQLKQKGLYAKEIAPAVEGGKRKLFSSGVSVPDLSLATRRLATLLGSAVPVY
EAVATLWEQEAPGELKKVLGRVRDRLAEGQGLAQSMAAEPAVFSESYIGMVAAGEASGALEVVLERLAEFQEDQAEVRSR
VITALIYPAIMVVVGTAVMLVLLGFVVPKISAVFETNKATLPMVTIILIKASALVRKGWWLLLAAAFALWTGYKRLIKSE
ELVRKRDRLILKLPVAGQLWQRLVLSRFAKVLGLLLQSGVPIIKAMDITGEAVVNREYKAFLAEARESLMQGGSLSASLK
ASPLFPPLLTHMTAVGEKSGELDGMLIKAGDAFQKEFNATVTRSMAMLEPLLILGMGLTIGFMVIAVLLPIMQLNQLVK

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=398, Percent_Identity=34.4221105527638, Blast_Score=251, Evalue=7e-68,
Organism=Escherichia coli, GI1786295, Length=328, Percent_Identity=27.1341463414634, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43196; Mature: 43065

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTFRYSAFNQKGTEVSGTVDAAGETEARLQLKQKGLYAKEIAPAVEGGKRKLFSSGVSV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
PDLSLATRRLATLLGSAVPVYEAVATLWEQEAPGELKKVLGRVRDRLAEGQGLAQSMAAE
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCC
PAVFSESYIGMVAAGEASGALEVVLERLAEFQEDQAEVRSRVITALIYPAIMVVVGTAVM
CCHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLLGFVVPKISAVFETNKATLPMVTIILIKASALVRKGWWLLLAAAFALWTGYKRLIKS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELVRKRDRLILKLPVAGQLWQRLVLSRFAKVLGLLLQSGVPIIKAMDITGEAVVNREYK
HHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
AFLAEARESLMQGGSLSASLKASPLFPPLLTHMTAVGEKSGELDGMLIKAGDAFQKEFNA
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHH
TVTRSMAMLEPLLILGMGLTIGFMVIAVLLPIMQLNQLVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PTFRYSAFNQKGTEVSGTVDAAGETEARLQLKQKGLYAKEIAPAVEGGKRKLFSSGVSV
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
PDLSLATRRLATLLGSAVPVYEAVATLWEQEAPGELKKVLGRVRDRLAEGQGLAQSMAAE
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCC
PAVFSESYIGMVAAGEASGALEVVLERLAEFQEDQAEVRSRVITALIYPAIMVVVGTAVM
CCHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLLGFVVPKISAVFETNKATLPMVTIILIKASALVRKGWWLLLAAAFALWTGYKRLIKS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELVRKRDRLILKLPVAGQLWQRLVLSRFAKVLGLLLQSGVPIIKAMDITGEAVVNREYK
HHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
AFLAEARESLMQGGSLSASLKASPLFPPLLTHMTAVGEKSGELDGMLIKAGDAFQKEFNA
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHH
TVTRSMAMLEPLLILGMGLTIGFMVIAVLLPIMQLNQLVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8326859 [H]