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The map label for this gene is gspE [H]

Identifier: 197116991

GI number: 197116991

Start: 712164

End: 713726

Strand: Reverse

Name: gspE [H]

Synonym: Gbem_0594

Alternate gene names: 197116991

Gene position: 713726-712164 (Counterclockwise)

Preceding gene: 308535177

Following gene: 197116990

Centisome position: 15.46

GC content: 64.49

Gene sequence:

>1563_bases
ATGGCAGAAAGCTTAGACATAGAAGAAATAGCGACACGGCTCGGCCTTCCCTACCAGGCCGAGATCGACGACTCCAAGGT
AGACGGGGCGCTGGTGAACCGGGTCCCGCTCAACTTCGCTCGCAACAACCTGCTGCTCCCGCTGCACGAGGAGGACGGCT
CCATCGTCGCCGCCAGCGCCGATCCGGCGAACCTCCTGGCGCTGGACGAGATGGCCGGGCTTTTCTCGCAGCCGGTCTCC
GTGGTCGCAGTCCCGCGCCCGGCTCTTCTGGATGCGGTGAACCGCCTCTACTCCCGCCTCTCCGGCTCCGCCCAGGAGGT
GGTCGAGGAACTCGAAGGGGAAGAGCTCTCCACCCTCGCCACCACCTTCAACGAACCGCGCGACCTGATGGAGCTGACCG
ACGAGGCGCCGGTGATCCGGCTTCTGAACTCGATCCTGTTCCAGGCGGTTAAGGAGCGCGCGAGCGACATCCACATCGAG
CCCTTCGAGCGCGAGCTCGAGGTCCGCTTCCGGATCGACGGCCTTTTGTACAAGATGCTCTCCCCCCCCAAGGTGATCCA
GGAGGCGCTCACCTCCCGCGTCAAGATCATGTCCGGGCTCAACATCGCCGAGAAGAGGCTGCCGCAGGACGGGCGCATCA
GGGTCAAGGTGGCCGGGCGCGACGTCGACATACGCGTCTCGCTCATCCCCACCTTCTTCGGGGAGCGCGTGGTATTGAGG
CTTTTGGACAAGCAGCGCGGCGTCCTCTCACTCAAAGAGATCGGCCTCTCCGACGGCAACGACCGGCTGATGGACCGGCT
CTTGTCGAGAACCAGCGGCATCATCCTGGTGACGGGACCTACCGGCAGCGGCAAGACCACAACGCTCTACGCGGCCCTCT
CCCAGATCAACTCGCCGGAGAAGAACATCATCACCGTCGAGGACCCGATCGAGTACCAGTTGAAGGGTGTGGGGCAGATC
CAAGTCAACCCGAAGATCGAACTCACCTTCGCCGCGGGGCTTCGCTCCATCCTCAGGCAGGACCCGGACATCGTCATGAT
CGGCGAGATCCGCGACGCCGAGACCGCCGAGATCGCCATGCAGGCCTCGCTCACCGGCCACCTGGTCCTCTCGACGCTGC
ACACAAACGACGCCGCCACCGCGGTGACCCGCCTGATCGACATGGGGATCGAGCCGTTCATGGTCGCCTCTTCGCTCTCC
GCGGTACTCGCGCAGCGGCTGGTCCGCGTCATCTGCCCGCACTGCAAGGAATCCTATGTCCCGGACAGAAGCTACCCCGG
CGTCGAACTCCCGCCGCTTCTGTACCGCGGCCGCGGCTGCGAGAAGTGCTTCAACCTTGGGACCATCGGGCGGGTCGGCA
TCTACGAACTTCTCCCCATCGACGCCGAACTCTGCTCCATGATCATCCGGCAGGCCTCCTCCGGCGCCATCAAGGAATAC
GCCGTCTCCAAGGGGATGCGCACCCTGCGCGAGGACGGCCTCGCCAAGGCAGCCCAGGGGATCACCACCATCGAGGAGGT
CCTGAGGGTGACTCAGGACGACTATGCCGACCTTTCGCTATAG

Upstream 100 bases:

>100_bases
AAAGCCCAAGTCGGTGACCGAGGACAAGCCCTAAGACCGGTGCGGAGCTGACCCTCCCACCGCAACCGGCAGCATCCAAC
CAAAAGCGAACAGGCCCAAC

Downstream 100 bases:

>100_bases
CGCCTTCAACCAGAAAGGGACCGAGGTCTCCGGGACCGTGGATGCGGCCGGCGAAACCGAGGCGCGGCTGCAACTGAAGC
AGAAGGGGCTCTACGCCAAG

Product: type II secretion system ATPase GspE

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MAESLDIEEIATRLGLPYQAEIDDSKVDGALVNRVPLNFARNNLLLPLHEEDGSIVAASADPANLLALDEMAGLFSQPVS
VVAVPRPALLDAVNRLYSRLSGSAQEVVEELEGEELSTLATTFNEPRDLMELTDEAPVIRLLNSILFQAVKERASDIHIE
PFERELEVRFRIDGLLYKMLSPPKVIQEALTSRVKIMSGLNIAEKRLPQDGRIRVKVAGRDVDIRVSLIPTFFGERVVLR
LLDKQRGVLSLKEIGLSDGNDRLMDRLLSRTSGIILVTGPTGSGKTTTLYAALSQINSPEKNIITVEDPIEYQLKGVGQI
QVNPKIELTFAAGLRSILRQDPDIVMIGEIRDAETAEIAMQASLTGHLVLSTLHTNDAATAVTRLIDMGIEPFMVASSLS
AVLAQRLVRVICPHCKESYVPDRSYPGVELPPLLYRGRGCEKCFNLGTIGRVGIYELLPIDAELCSMIIRQASSGAIKEY
AVSKGMRTLREDGLAKAAQGITTIEEVLRVTQDDYADLSL

Sequences:

>Translated_520_residues
MAESLDIEEIATRLGLPYQAEIDDSKVDGALVNRVPLNFARNNLLLPLHEEDGSIVAASADPANLLALDEMAGLFSQPVS
VVAVPRPALLDAVNRLYSRLSGSAQEVVEELEGEELSTLATTFNEPRDLMELTDEAPVIRLLNSILFQAVKERASDIHIE
PFERELEVRFRIDGLLYKMLSPPKVIQEALTSRVKIMSGLNIAEKRLPQDGRIRVKVAGRDVDIRVSLIPTFFGERVVLR
LLDKQRGVLSLKEIGLSDGNDRLMDRLLSRTSGIILVTGPTGSGKTTTLYAALSQINSPEKNIITVEDPIEYQLKGVGQI
QVNPKIELTFAAGLRSILRQDPDIVMIGEIRDAETAEIAMQASLTGHLVLSTLHTNDAATAVTRLIDMGIEPFMVASSLS
AVLAQRLVRVICPHCKESYVPDRSYPGVELPPLLYRGRGCEKCFNLGTIGRVGIYELLPIDAELCSMIIRQASSGAIKEY
AVSKGMRTLREDGLAKAAQGITTIEEVLRVTQDDYADLSL
>Mature_519_residues
AESLDIEEIATRLGLPYQAEIDDSKVDGALVNRVPLNFARNNLLLPLHEEDGSIVAASADPANLLALDEMAGLFSQPVSV
VAVPRPALLDAVNRLYSRLSGSAQEVVEELEGEELSTLATTFNEPRDLMELTDEAPVIRLLNSILFQAVKERASDIHIEP
FERELEVRFRIDGLLYKMLSPPKVIQEALTSRVKIMSGLNIAEKRLPQDGRIRVKVAGRDVDIRVSLIPTFFGERVVLRL
LDKQRGVLSLKEIGLSDGNDRLMDRLLSRTSGIILVTGPTGSGKTTTLYAALSQINSPEKNIITVEDPIEYQLKGVGQIQ
VNPKIELTFAAGLRSILRQDPDIVMIGEIRDAETAEIAMQASLTGHLVLSTLHTNDAATAVTRLIDMGIEPFMVASSLSA
VLAQRLVRVICPHCKESYVPDRSYPGVELPPLLYRGRGCEKCFNLGTIGRVGIYELLPIDAELCSMIIRQASSGAIKEYA
VSKGMRTLREDGLAKAAQGITTIEEVLRVTQDDYADLSL

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=482, Percent_Identity=44.6058091286307, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1786296, Length=439, Percent_Identity=41.6856492027335, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI87082188, Length=279, Percent_Identity=33.6917562724014, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 56987; Mature: 56855

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESLDIEEIATRLGLPYQAEIDDSKVDGALVNRVPLNFARNNLLLPLHEEDGSIVAASA
CCCCCCHHHHHHHHCCCEEECCCCCCCCCHHHHCCCCHHCCCCCEEEEECCCCCEEEECC
DPANLLALDEMAGLFSQPVSVVAVPRPALLDAVNRLYSRLSGSAQEVVEELEGEELSTLA
CCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHH
TTFNEPRDLMELTDEAPVIRLLNSILFQAVKERASDIHIEPFERELEVRFRIDGLLYKML
HHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHC
SPPKVIQEALTSRVKIMSGLNIAEKRLPQDGRIRVKVAGRDVDIRVSLIPTFFGERVVLR
CCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCCEEEEEEEECHHHHHHHHHH
LLDKQRGVLSLKEIGLSDGNDRLMDRLLSRTSGIILVTGPTGSGKTTTLYAALSQINSPE
HHHHCCCCEEHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCC
KNIITVEDPIEYQLKGVGQIQVNPKIELTFAAGLRSILRQDPDIVMIGEIRDAETAEIAM
CCEEEECCCCCHHCCCCEEEEECCEEEEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
QASLTGHLVLSTLHTNDAATAVTRLIDMGIEPFMVASSLSAVLAQRLVRVICPHCKESYV
HHCCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
PDRSYPGVELPPLLYRGRGCEKCFNLGTIGRVGIYELLPIDAELCSMIIRQASSGAIKEY
CCCCCCCCCCCHHHHCCCCHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCHHHHH
AVSKGMRTLREDGLAKAAQGITTIEEVLRVTQDDYADLSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AESLDIEEIATRLGLPYQAEIDDSKVDGALVNRVPLNFARNNLLLPLHEEDGSIVAASA
CCCCCHHHHHHHHCCCEEECCCCCCCCCHHHHCCCCHHCCCCCEEEEECCCCCEEEECC
DPANLLALDEMAGLFSQPVSVVAVPRPALLDAVNRLYSRLSGSAQEVVEELEGEELSTLA
CCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHH
TTFNEPRDLMELTDEAPVIRLLNSILFQAVKERASDIHIEPFERELEVRFRIDGLLYKML
HHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHC
SPPKVIQEALTSRVKIMSGLNIAEKRLPQDGRIRVKVAGRDVDIRVSLIPTFFGERVVLR
CCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCCEEEEEEEECHHHHHHHHHH
LLDKQRGVLSLKEIGLSDGNDRLMDRLLSRTSGIILVTGPTGSGKTTTLYAALSQINSPE
HHHHCCCCEEHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCC
KNIITVEDPIEYQLKGVGQIQVNPKIELTFAAGLRSILRQDPDIVMIGEIRDAETAEIAM
CCEEEECCCCCHHCCCCEEEEECCEEEEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
QASLTGHLVLSTLHTNDAATAVTRLIDMGIEPFMVASSLSAVLAQRLVRVICPHCKESYV
HHCCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
PDRSYPGVELPPLLYRGRGCEKCFNLGTIGRVGIYELLPIDAELCSMIIRQASSGAIKEY
CCCCCCCCCCCHHHHCCCCHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCHHHHH
AVSKGMRTLREDGLAKAAQGITTIEEVLRVTQDDYADLSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]