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The map label for this gene is ilvE [H]

Identifier: 197116969

GI number: 197116969

Start: 694786

End: 695856

Strand: Reverse

Name: ilvE [H]

Synonym: Gbem_0572

Alternate gene names: 197116969

Gene position: 695856-694786 (Counterclockwise)

Preceding gene: 197116971

Following gene: 197116967

Centisome position: 15.08

GC content: 60.69

Gene sequence:

>1071_bases
ATGGAAATCAGGATAGTGCCCCTCAGCGAGGGGGAGAAAAAGGCGAAATTCAAAGACGAATCACAGCTTGGCTTTGGCAA
GATTTTCACCGACCGCATGCTTTTGATCGAGTGGAAGGTCGGCCAGGGGTGGGTAGATGCCAGGATCAAGAAGTACGAGC
CGTTCCAGCTCGACCCGGCGGCGCTCGTGTTGCATTATGCCCAGGAAATCTTCGAAGGGCTCAAGGCCTACAGGTGGAAG
GACGGCACCATTGCTCTCTTCCGTCCCGAGATGAACGCCCGCCGCTTCAACCACTCAGCGGACCGGCTCTGCATGCCCGA
GATCCCCGAGGAACTCTTTGTAAGCGGCATCGAGCAGTTGGTGGCCGCCGAGCGCGAATGGGTCCCCGGCGCCGAAGGGA
CCTCCCTCTACATCCGCCCCGCCATGATCGCCGTGGAGCCGCATGTCGGCATCAAGCCTTCGGACCACTACTACTTCTAC
GTGATTCTCTCCCCGGTCGGCGCCTACTACGCCAACGGCTTCAATCCCGTGAAGATCATGGTGGAGGACCATTACGTGAG
GGCAACCCCGGGCGGCACCGGCGAGGCCAAGACCGGCGGCAACTACGCCAGCTCGCTCAAGGCGGGACTCGAGGCCAAGA
AGAAAGGGTTCGACCAGGTGCTCTGGCTGGACGGCGTCCACAAGCGTTACATCGAGGAAGTCGGCTCGATGAACATGTTC
TTTGCCTACGGCGACACCATCGTCACCGCGCCGCTTGAAGGAAGCATACTCAACGGCATCACCCGTGACTCGGTGCTGAC
CCTGGCCAAGTCGTTGAACCTGAAGGTCGAGGAGCGGCGCATAGACGTCAACGACCTGATGGCGGATCTGAAAAGCGGGA
AGATCACCGAGGCATTCGGCAGCGGCACCGCCGCGGTGGTAACCCCGGTCGGTACCCTGAGCTACGCCGGCGATACGGTC
CAGGTGGGCAACGGCGGCGTAGGGAAATACACCCAGGTGCTCTACGACACGCTGACCGGGATCCAGACCGGCAAGATCGA
AGACCGATTCGGCTGGATCAGGAAGATCTAG

Upstream 100 bases:

>100_bases
TGCGGCGGGTTAAATTGCTTGCGCCCCCTATCCTGAATATGGTAAACGAAAAGATCGAATAAAAGCGACGCCTGGCGCCG
AAAACCGGAGGTTCTGCCAC

Downstream 100 bases:

>100_bases
CCTCAAAAACAGGATGGCCGCGCGGGTGATTCCCGCGCGGCCATCGTCATTTATAAGAAGAAGAGCTGCCCCCCTCCTGC
TGCTGTGCCCAAACGCAACT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85 [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MEIRIVPLSEGEKKAKFKDESQLGFGKIFTDRMLLIEWKVGQGWVDARIKKYEPFQLDPAALVLHYAQEIFEGLKAYRWK
DGTIALFRPEMNARRFNHSADRLCMPEIPEELFVSGIEQLVAAEREWVPGAEGTSLYIRPAMIAVEPHVGIKPSDHYYFY
VILSPVGAYYANGFNPVKIMVEDHYVRATPGGTGEAKTGGNYASSLKAGLEAKKKGFDQVLWLDGVHKRYIEEVGSMNMF
FAYGDTIVTAPLEGSILNGITRDSVLTLAKSLNLKVEERRIDVNDLMADLKSGKITEAFGSGTAAVVTPVGTLSYAGDTV
QVGNGGVGKYTQVLYDTLTGIQTGKIEDRFGWIRKI

Sequences:

>Translated_356_residues
MEIRIVPLSEGEKKAKFKDESQLGFGKIFTDRMLLIEWKVGQGWVDARIKKYEPFQLDPAALVLHYAQEIFEGLKAYRWK
DGTIALFRPEMNARRFNHSADRLCMPEIPEELFVSGIEQLVAAEREWVPGAEGTSLYIRPAMIAVEPHVGIKPSDHYYFY
VILSPVGAYYANGFNPVKIMVEDHYVRATPGGTGEAKTGGNYASSLKAGLEAKKKGFDQVLWLDGVHKRYIEEVGSMNMF
FAYGDTIVTAPLEGSILNGITRDSVLTLAKSLNLKVEERRIDVNDLMADLKSGKITEAFGSGTAAVVTPVGTLSYAGDTV
QVGNGGVGKYTQVLYDTLTGIQTGKIEDRFGWIRKI
>Mature_356_residues
MEIRIVPLSEGEKKAKFKDESQLGFGKIFTDRMLLIEWKVGQGWVDARIKKYEPFQLDPAALVLHYAQEIFEGLKAYRWK
DGTIALFRPEMNARRFNHSADRLCMPEIPEELFVSGIEQLVAAEREWVPGAEGTSLYIRPAMIAVEPHVGIKPSDHYYFY
VILSPVGAYYANGFNPVKIMVEDHYVRATPGGTGEAKTGGNYASSLKAGLEAKKKGFDQVLWLDGVHKRYIEEVGSMNMF
FAYGDTIVTAPLEGSILNGITRDSVLTLAKSLNLKVEERRIDVNDLMADLKSGKITEAFGSGTAAVVTPVGTLSYAGDTV
QVGNGGVGKYTQVLYDTLTGIQTGKIEDRFGWIRKI

Specific function: Transaminates branched-chain amino acids and ketoglutarate [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=337, Percent_Identity=43.3234421364985, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI296010906, Length=337, Percent_Identity=43.3234421364985, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI38176287, Length=337, Percent_Identity=43.3234421364985, Blast_Score=262, Evalue=3e-70,
Organism=Homo sapiens, GI50658084, Length=307, Percent_Identity=40.3908794788274, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI296010902, Length=340, Percent_Identity=37.9411764705882, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI296010900, Length=340, Percent_Identity=37.9411764705882, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI258614015, Length=261, Percent_Identity=39.463601532567, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI48994963, Length=318, Percent_Identity=31.7610062893082, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17568601, Length=358, Percent_Identity=38.268156424581, Blast_Score=246, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI17565728, Length=345, Percent_Identity=36.8115942028986, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6322608, Length=355, Percent_Identity=40.5633802816901, Blast_Score=276, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6322002, Length=353, Percent_Identity=40.2266288951841, Blast_Score=265, Evalue=9e-72,
Organism=Drosophila melanogaster, GI24641779, Length=343, Percent_Identity=41.399416909621, Blast_Score=254, Evalue=7e-68,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39489; Mature: 39489

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRIVPLSEGEKKAKFKDESQLGFGKIFTDRMLLIEWKVGQGWVDARIKKYEPFQLDPA
CEEEEEECCCCCCCCCCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCHH
ALVLHYAQEIFEGLKAYRWKDGTIALFRPEMNARRFNHSADRLCMPEIPEELFVSGIEQL
HHHHHHHHHHHHHHHHEECCCCCEEEECCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VAAEREWVPGAEGTSLYIRPAMIAVEPHVGIKPSDHYYFYVILSPVGAYYANGFNPVKIM
HHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCHHHHHHCCCCEEEEE
VEDHYVRATPGGTGEAKTGGNYASSLKAGLEAKKKGFDQVLWLDGVHKRYIEEVGSMNMF
EECCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCEEE
FAYGDTIVTAPLEGSILNGITRDSVLTLAKSLNLKVEERRIDVNDLMADLKSGKITEAFG
EEECCEEEECCCCCHHHCCCCHHHHHHHHHHCCCEEHHHCCCHHHHHHHHCCCCEEEHHC
SGTAAVVTPVGTLSYAGDTVQVGNGGVGKYTQVLYDTLTGIQTGKIEDRFGWIRKI
CCCEEEEECCCHHEECCCEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MEIRIVPLSEGEKKAKFKDESQLGFGKIFTDRMLLIEWKVGQGWVDARIKKYEPFQLDPA
CEEEEEECCCCCCCCCCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCHH
ALVLHYAQEIFEGLKAYRWKDGTIALFRPEMNARRFNHSADRLCMPEIPEELFVSGIEQL
HHHHHHHHHHHHHHHHEECCCCCEEEECCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VAAEREWVPGAEGTSLYIRPAMIAVEPHVGIKPSDHYYFYVILSPVGAYYANGFNPVKIM
HHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCHHHHHHCCCCEEEEE
VEDHYVRATPGGTGEAKTGGNYASSLKAGLEAKKKGFDQVLWLDGVHKRYIEEVGSMNMF
EECCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCEEE
FAYGDTIVTAPLEGSILNGITRDSVLTLAKSLNLKVEERRIDVNDLMADLKSGKITEAFG
EEECCEEEECCCCCHHHCCCCHHHHHHHHHHCCCEEHHHCCCHHHHHHHHCCCCEEEHHC
SGTAAVVTPVGTLSYAGDTVQVGNGGVGKYTQVLYDTLTGIQTGKIEDRFGWIRKI
CCCEEEEECCCHHEECCCEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659 [H]