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The map label for this gene is clpB [H]

Identifier: 197116967

GI number: 197116967

Start: 691256

End: 693850

Strand: Reverse

Name: clpB [H]

Synonym: Gbem_0570

Alternate gene names: 197116967

Gene position: 693850-691256 (Counterclockwise)

Preceding gene: 197116969

Following gene: 197116966

Centisome position: 15.03

GC content: 63.39

Gene sequence:

>2595_bases
ATGATACGACCGGAAAAAATGACCGTTAAGACACAGGAAGCACTGGTGACGGCGCAGGAGAAAGCGGCAGAGATGTCCGC
CGGCAGCATCGAGGCCGAACACCTGCTGCTCGCCCTCATAGACCAGGAGGGAGGGTTCGTCCCCGACCTTTTGAAGAAAA
TAGGAGCGCCGCTGGCCAAGCTGCGCGAGAGCGTCGACGAGACGCTGAAGCGTCTGCCCAAGGTCACCGGAGCTGCCCAG
GTCTCGCTCTCCGCCACGCTGAACCGGGTACTGGACCAGGCCCAGAAAGAGGCGGACTCCATGCACGACGCGTTCGTCTC
CACGGAGCACCTGCTTTTGGCGCTGGTGGCAGCGAAGGACGCACCGAGTTCCCGGCTCTTGAGCCAGAACGGGGTTACCC
GCGACGGGATCCTGTCGGCGCTCAAAGAACTGCGCGGCGGCGAGGCGGTGAACGAGCAGGACCCGGAGGGGAAATACCAG
GCGCTTGCCAAGTACTGTCGCGACCTCACCGACCTGGCAAGGCAGGGGAAACTCGACCCGGTGATCGGGCGCGACGACGA
AGTAAGAAGGGTGCTCCAGGTCCTGTCGCGCCGCACCAAGAACAACCCGGTGCTGATCGGGGAGCCTGGCGTCGGCAAGA
CCGCCATCGTCGAGGGACTCGCCCAGCGCATCGTTTCGGGCGACGTCCCCGAATCGCTCAAGGACAAGGTGATCATGGCG
CTCGACATGGGGGCGCTGATCGCAGGGGCGAAGTACCGCGGCGAGTTCGAGGAGCGCCTGAAGGCGGTGATCAAGGAGGT
GGCGCGCTCGGAAGGTAAGATCATCCTCTTCATCGACGAGATGCACACGCTGGTGGGAGCCGGTGCCGCCGAGGGGGCGA
TGGACGCCTCCAACATGCTGAAGCCGGCCCTGGCCCGCGGAGAGCTGCACTGCATCGGCGCCACGACCCTCAACGAATAC
CGCAAGCACATCGAGAAGGACGCCGCCCTGGAACGCCGCTTCCAGCAGGTATTCGCCGGCGAGCCGAGCGTGGAGGAGAC
CATCTCCATCCTCAGGGGGCTCAAGGAGAAGTACGAGAACTACCACAAGATACGGATCAAGGACGGCGCCATCATCGCCG
CGGCCACCCTTTCGGACCGCTACATCACCGACCGCTTCCTCCCCGACAAAGCGATCGACCTGATCGACGAGGCCGCGTCC
CGCCTACGGATCGAGATCGATTCGAAGCCGACCGAGATCGACGAAATCGACCGGCGCATCATCCAGCTCGAGATCGAGCG
CCAGGCCATGCTGCGCGAGCAGGACACGAAGGTGCTGGACCGGCTCAAAAAGCTAGAGGAGGAACTGGACGGGTTGAAGG
CGCAGTCGGCGAAGCTGAACGAACATTGGAAAAGGGAGACCAACCTCCTTAAGGATTTGGGGGAGCTGAAGCAGCGCAAG
GAGGAGAAAGCCGAGGAAGCCAAGCGTGCGGAGCGCGACGGGCTCTTGGCCCGCACCGCCGAGATCCGCTACGGCGAGAT
ACCCGCCATCGAGAAGGAGATGGAGGAGAAGCGGCTCACCCTGGAGTCGGTCCAGAAGGAAGAGAAGATGCTCCCGGAAG
AAGTCGACGCCGAGATGGTGGCCGAGGTGGTGGCCAAGTGGACCGGCATACCGGTGAACCGGATGCTGGAGACCGAGTCC
GACAAGCTGGTCAAGATGGAGGAGCGGCTCAAAAGCCGCGTGGTGGGGCAGGACGAGGCGCTCACCCTGGTGGCGAACGC
GGTGCGGCGCGCCCGCAGCGGCCTATCCGACCCCAACCGCCCCATCGGCTCCTTCATCTTCCTGGGACCTACCGGCGTCG
GCAAGACCGAGACGGCACGGGCCTTGGCCTCGTTTCTCTTCGACGACGATCAGGCCATCGTCCGCATCGACATGAGCGAA
TACCAGGAGAAGCACACGGTCGCCCGCCTGATCGGAGCCCCTCCCGGGTACGTCGGCTACGAGGAGGGAGGGCAACTCAC
CGAGGCGGTGCGCCGCCGCCCCTACTGCATCGTCCTATTCGACGAGATCGAGAAGGCGCACCCCGAGGTGTTCAACGTCT
TCCTGCAAATATTGGACGACGGCCGGCTCACCGACGGCCAGGGGCGGACGGTCGATTTCAAGAACTCGGTCATCATCATG
ACCAGCAACCTGGGGTCGCAGTGGATCCAGCAGTACGGGGCCGCCGACTACGGAAAAATGCAAAGCGAGGTCATGGAGAC
CCTGCGGGAGGCATTCAAGCCCGAGTTCCTGAACCGGGTCGACGAGATCGTCATCTACCACTCGCTGCCGCTGGAAAGGA
TCAAGGAAATCGTCTCCATCCAATTGCAGTCGCTCACCAAAAGACTGGAGGAAAAGGGACTCGGCTTGGAGGTGACCGAG
CAGGCTCGCGAATTCCTGGCCCGGGAGGGATACGACCCTGCCTACGGGGCAAGGCCGCTCAAGCGCGCGCTGCAAAGGAA
GATCCAGGACCCGCTGGCGCTCATGCTGCTTGAGAACAAGTTCGCCCCGGGCGACATAGTGGTGGTCGACCTCCCGGCGC
AGGGAGACTCCCTGACCATCGGGAAAAAGGCATGA

Upstream 100 bases:

>100_bases
CGGGCGCTCCCGCCCATACTCTCAAGCTAAAAACCTCGCCCAGCCTTGACACTACCCATCCCCCTACTTATCTTCTTCTA
GGACTTTAGGAGGCACTGCC

Downstream 100 bases:

>100_bases
TGCTGTAAACGGTAATTAATAAATTGCCAACTTTCGGCAAAGCTGATAGTGTACGCGGCGCAAAGCTGCTGCTTTGCGCC
GCGCTTGCGGCACCTACCCT

Product: ATP-dependent chaperone ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 864; Mature: 864

Protein sequence:

>864_residues
MIRPEKMTVKTQEALVTAQEKAAEMSAGSIEAEHLLLALIDQEGGFVPDLLKKIGAPLAKLRESVDETLKRLPKVTGAAQ
VSLSATLNRVLDQAQKEADSMHDAFVSTEHLLLALVAAKDAPSSRLLSQNGVTRDGILSALKELRGGEAVNEQDPEGKYQ
ALAKYCRDLTDLARQGKLDPVIGRDDEVRRVLQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVSGDVPESLKDKVIMA
LDMGALIAGAKYRGEFEERLKAVIKEVARSEGKIILFIDEMHTLVGAGAAEGAMDASNMLKPALARGELHCIGATTLNEY
RKHIEKDAALERRFQQVFAGEPSVEETISILRGLKEKYENYHKIRIKDGAIIAAATLSDRYITDRFLPDKAIDLIDEAAS
RLRIEIDSKPTEIDEIDRRIIQLEIERQAMLREQDTKVLDRLKKLEEELDGLKAQSAKLNEHWKRETNLLKDLGELKQRK
EEKAEEAKRAERDGLLARTAEIRYGEIPAIEKEMEEKRLTLESVQKEEKMLPEEVDAEMVAEVVAKWTGIPVNRMLETES
DKLVKMEERLKSRVVGQDEALTLVANAVRRARSGLSDPNRPIGSFIFLGPTGVGKTETARALASFLFDDDQAIVRIDMSE
YQEKHTVARLIGAPPGYVGYEEGGQLTEAVRRRPYCIVLFDEIEKAHPEVFNVFLQILDDGRLTDGQGRTVDFKNSVIIM
TSNLGSQWIQQYGAADYGKMQSEVMETLREAFKPEFLNRVDEIVIYHSLPLERIKEIVSIQLQSLTKRLEEKGLGLEVTE
QAREFLAREGYDPAYGARPLKRALQRKIQDPLALMLLENKFAPGDIVVVDLPAQGDSLTIGKKA

Sequences:

>Translated_864_residues
MIRPEKMTVKTQEALVTAQEKAAEMSAGSIEAEHLLLALIDQEGGFVPDLLKKIGAPLAKLRESVDETLKRLPKVTGAAQ
VSLSATLNRVLDQAQKEADSMHDAFVSTEHLLLALVAAKDAPSSRLLSQNGVTRDGILSALKELRGGEAVNEQDPEGKYQ
ALAKYCRDLTDLARQGKLDPVIGRDDEVRRVLQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVSGDVPESLKDKVIMA
LDMGALIAGAKYRGEFEERLKAVIKEVARSEGKIILFIDEMHTLVGAGAAEGAMDASNMLKPALARGELHCIGATTLNEY
RKHIEKDAALERRFQQVFAGEPSVEETISILRGLKEKYENYHKIRIKDGAIIAAATLSDRYITDRFLPDKAIDLIDEAAS
RLRIEIDSKPTEIDEIDRRIIQLEIERQAMLREQDTKVLDRLKKLEEELDGLKAQSAKLNEHWKRETNLLKDLGELKQRK
EEKAEEAKRAERDGLLARTAEIRYGEIPAIEKEMEEKRLTLESVQKEEKMLPEEVDAEMVAEVVAKWTGIPVNRMLETES
DKLVKMEERLKSRVVGQDEALTLVANAVRRARSGLSDPNRPIGSFIFLGPTGVGKTETARALASFLFDDDQAIVRIDMSE
YQEKHTVARLIGAPPGYVGYEEGGQLTEAVRRRPYCIVLFDEIEKAHPEVFNVFLQILDDGRLTDGQGRTVDFKNSVIIM
TSNLGSQWIQQYGAADYGKMQSEVMETLREAFKPEFLNRVDEIVIYHSLPLERIKEIVSIQLQSLTKRLEEKGLGLEVTE
QAREFLAREGYDPAYGARPLKRALQRKIQDPLALMLLENKFAPGDIVVVDLPAQGDSLTIGKKA
>Mature_864_residues
MIRPEKMTVKTQEALVTAQEKAAEMSAGSIEAEHLLLALIDQEGGFVPDLLKKIGAPLAKLRESVDETLKRLPKVTGAAQ
VSLSATLNRVLDQAQKEADSMHDAFVSTEHLLLALVAAKDAPSSRLLSQNGVTRDGILSALKELRGGEAVNEQDPEGKYQ
ALAKYCRDLTDLARQGKLDPVIGRDDEVRRVLQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVSGDVPESLKDKVIMA
LDMGALIAGAKYRGEFEERLKAVIKEVARSEGKIILFIDEMHTLVGAGAAEGAMDASNMLKPALARGELHCIGATTLNEY
RKHIEKDAALERRFQQVFAGEPSVEETISILRGLKEKYENYHKIRIKDGAIIAAATLSDRYITDRFLPDKAIDLIDEAAS
RLRIEIDSKPTEIDEIDRRIIQLEIERQAMLREQDTKVLDRLKKLEEELDGLKAQSAKLNEHWKRETNLLKDLGELKQRK
EEKAEEAKRAERDGLLARTAEIRYGEIPAIEKEMEEKRLTLESVQKEEKMLPEEVDAEMVAEVVAKWTGIPVNRMLETES
DKLVKMEERLKSRVVGQDEALTLVANAVRRARSGLSDPNRPIGSFIFLGPTGVGKTETARALASFLFDDDQAIVRIDMSE
YQEKHTVARLIGAPPGYVGYEEGGQLTEAVRRRPYCIVLFDEIEKAHPEVFNVFLQILDDGRLTDGQGRTVDFKNSVIIM
TSNLGSQWIQQYGAADYGKMQSEVMETLREAFKPEFLNRVDEIVIYHSLPLERIKEIVSIQLQSLTKRLEEKGLGLEVTE
QAREFLAREGYDPAYGARPLKRALQRKIQDPLALMLLENKFAPGDIVVVDLPAQGDSLTIGKKA

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=314, Percent_Identity=36.624203821656, Blast_Score=213, Evalue=9e-55,
Organism=Escherichia coli, GI1788943, Length=848, Percent_Identity=57.311320754717, Blast_Score=979, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=455, Percent_Identity=39.5604395604396, Blast_Score=291, Evalue=9e-80,
Organism=Saccharomyces cerevisiae, GI6320464, Length=704, Percent_Identity=50.8522727272727, Blast_Score=718, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=855, Percent_Identity=42.6900584795322, Blast_Score=664, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96381; Mature: 96381

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPEKMTVKTQEALVTAQEKAAEMSAGSIEAEHLLLALIDQEGGFVPDLLKKIGAPLAK
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCHHHHHHHHCCCHHH
LRESVDETLKRLPKVTGAAQVSLSATLNRVLDQAQKEADSMHDAFVSTEHLLLALVAAKD
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
APSSRLLSQNGVTRDGILSALKELRGGEAVNEQDPEGKYQALAKYCRDLTDLARQGKLDP
CCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
VIGRDDEVRRVLQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVSGDVPESLKDKVIMA
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHEEHH
LDMGALIAGAKYRGEFEERLKAVIKEVARSEGKIILFIDEMHTLVGAGAAEGAMDASNML
CCHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCHHHHH
KPALARGELHCIGATTLNEYRKHIEKDAALERRFQQVFAGEPSVEETISILRGLKEKYEN
HHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
YHKIRIKDGAIIAAATLSDRYITDRFLPDKAIDLIDEAASRLRIEIDSKPTEIDEIDRRI
CEEEEEECCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHH
IQLEIERQAMLREQDTKVLDRLKKLEEELDGLKAQSAKLNEHWKRETNLLKDLGELKQRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEKAEEAKRAERDGLLARTAEIRYGEIPAIEKEMEEKRLTLESVQKEEKMLPEEVDAEMV
HHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHH
AEVVAKWTGIPVNRMLETESDKLVKMEERLKSRVVGQDEALTLVANAVRRARSGLSDPNR
HHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
PIGSFIFLGPTGVGKTETARALASFLFDDDQAIVRIDMSEYQEKHTVARLIGAPPGYVGY
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCC
EEGGQLTEAVRRRPYCIVLFDEIEKAHPEVFNVFLQILDDGRLTDGQGRTVDFKNSVIIM
CCCCHHHHHHHHCCEEEEEEHHHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEE
TSNLGSQWIQQYGAADYGKMQSEVMETLREAFKPEFLNRVDEIVIYHSLPLERIKEIVSI
ECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHH
QLQSLTKRLEEKGLGLEVTEQAREFLAREGYDPAYGARPLKRALQRKIQDPLALMLLENK
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCC
FAPGDIVVVDLPAQGDSLTIGKKA
CCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MIRPEKMTVKTQEALVTAQEKAAEMSAGSIEAEHLLLALIDQEGGFVPDLLKKIGAPLAK
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCHHHHHHHHCCCHHH
LRESVDETLKRLPKVTGAAQVSLSATLNRVLDQAQKEADSMHDAFVSTEHLLLALVAAKD
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
APSSRLLSQNGVTRDGILSALKELRGGEAVNEQDPEGKYQALAKYCRDLTDLARQGKLDP
CCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
VIGRDDEVRRVLQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVSGDVPESLKDKVIMA
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHEEHH
LDMGALIAGAKYRGEFEERLKAVIKEVARSEGKIILFIDEMHTLVGAGAAEGAMDASNML
CCHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCHHHHH
KPALARGELHCIGATTLNEYRKHIEKDAALERRFQQVFAGEPSVEETISILRGLKEKYEN
HHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
YHKIRIKDGAIIAAATLSDRYITDRFLPDKAIDLIDEAASRLRIEIDSKPTEIDEIDRRI
CEEEEEECCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHH
IQLEIERQAMLREQDTKVLDRLKKLEEELDGLKAQSAKLNEHWKRETNLLKDLGELKQRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEKAEEAKRAERDGLLARTAEIRYGEIPAIEKEMEEKRLTLESVQKEEKMLPEEVDAEMV
HHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHH
AEVVAKWTGIPVNRMLETESDKLVKMEERLKSRVVGQDEALTLVANAVRRARSGLSDPNR
HHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
PIGSFIFLGPTGVGKTETARALASFLFDDDQAIVRIDMSEYQEKHTVARLIGAPPGYVGY
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCC
EEGGQLTEAVRRRPYCIVLFDEIEKAHPEVFNVFLQILDDGRLTDGQGRTVDFKNSVIIM
CCCCHHHHHHHHCCEEEEEEHHHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEE
TSNLGSQWIQQYGAADYGKMQSEVMETLREAFKPEFLNRVDEIVIYHSLPLERIKEIVSI
ECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHH
QLQSLTKRLEEKGLGLEVTEQAREFLAREGYDPAYGARPLKRALQRKIQDPLALMLLENK
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCC
FAPGDIVVVDLPAQGDSLTIGKKA
CCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA