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The map label for this gene is glcD-1 [H]

Identifier: 197116961

GI number: 197116961

Start: 681562

End: 682941

Strand: Reverse

Name: glcD-1 [H]

Synonym: Gbem_0564

Alternate gene names: 197116961

Gene position: 682941-681562 (Counterclockwise)

Preceding gene: 308535174

Following gene: 197116960

Centisome position: 14.8

GC content: 63.19

Gene sequence:

>1380_bases
ATGCTCGACGCACGCATACTGCAGCAATTGACCGATATCGTCGGCAGCGAGAACATCGCCGCCACCCCGCAGGACCTGAT
CTGCTACGGCTACGACGCCACCCAAATGGAATTCCTCCCCGATTGCGTAGTGCATCCCGACGGCAGCGAAGAGGTAGCGC
GGATACTGAAGCTCGCCAACAGTGAAGGCTTCCCCGTATTCCCCAGGGGGGCAGGCAGCGGCTTCACCGGCGGCGCCCTC
CCGAAAGGGGGGGGAGTGGTGCTGGTGACCACCCGCATGAACCGGATCCTCAGGATCGACACCGACAACCTGGTGGCCGA
AGTGGAGCCGGGGGTCGTGACCGAGACCTTCCAGATGGCGGTCGAGAAACTGGGGCTCTTCTATCCCCCCGACCCCGCCT
CCCTCAAGTTTTCCACGTTGGGCGGCAACGTCGCCGAGTGCGCCGGCGGCCCGCGCGCCGTCAAGTACGGCGTCACCAAG
GATTTCGTGATGGGGCTGGAAGTGGTCCTTCCCACCGGCGAGGTGATCAGGACCGGCGGGGAGACGGTGAAGGGAGTGGT
GGGTTACGACCTCACCAAGCTCATCTGCGGCTCGGAAGGGACGCTCGGGGTGATCACCAAGATCATCTTCAAGCTCCTCC
CCTACCCCGACGCCAAGAAGACCATGCTGACCGTCTTCGATTCCATCGACGGCGCCGCCCGCGCGGTCTCGACCATCATC
AAGAACAAGATCATCCCGACGACGCTCGAATTCATGGACCATGCCACGCTCGTCTGCGTCGAGCGCCGTTTCAACCTGGG
GCTACCCGACAGCGGCCGCGCCGTGCTTCTCATCGAGGTGGACGGCGACCGCGACCTGATCGAAAAGCAGGCGGGAGAAA
TCCACAAGTTGATCGAGCCGCTCGGCCTGGTGCAGTTCCGCGTCGCCAAGGACGCGGCCGAGAGCGAGGAACTGTGGCGG
GTGCGCCGCCTGGTCTCCCCGTCTTTAAGGGACGTGAACCCGGACAAGTTCAACGAGGACATCGTGGTGCCGAGGAGCAA
GGTTCCCGACGTCATCAGGCGCATCGAGGTGATCCAGAAGCGCTATGACATCCCCATCGTCAACTTCGGCCACGCAGGCG
ACGGCAACATCCACGTCAACATCATGATCGATAAGCAAGTGCCGGGGCAGTTGGAGAAGGCGCACGAGGCGATCAAGGAA
GTGTTCCAGGCAGCGCTCGACCTGGGCGGGACCATGTCCGGCGAGCACGGCGTCGGGCTTGCCAAGCAGCCGTACATACC
GATGGAGCTTACCGCCGACCAGGTGGCGGCGATGAAAGCGATCAAGCGAGCGCTCGACCCGAACAACATCCTAAACCCCG
GCAAGATGTTGCCGGTGTGA

Upstream 100 bases:

>100_bases
TGATACCCGAGGGGGGGAACGTGCTGCCGGTGCACTCTCCGGCGTAACTGCCGCCACAAATATGAAACGAGATCCGAAAG
ACCCATCGAAAGGAAGATAG

Downstream 100 bases:

>100_bases
ACCAATTGACAATTGACGATTGACAATTGACAACGTTCCCTCGTCCACCTGGTCCATTGCGTCCACAAGGTCCACGTGGT
CCATTTGCAGCAACCCAATG

Product: D-lactate/glycolate dehydrogenase FAD-binding protein

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLDARILQQLTDIVGSENIAATPQDLICYGYDATQMEFLPDCVVHPDGSEEVARILKLANSEGFPVFPRGAGSGFTGGAL
PKGGGVVLVTTRMNRILRIDTDNLVAEVEPGVVTETFQMAVEKLGLFYPPDPASLKFSTLGGNVAECAGGPRAVKYGVTK
DFVMGLEVVLPTGEVIRTGGETVKGVVGYDLTKLICGSEGTLGVITKIIFKLLPYPDAKKTMLTVFDSIDGAARAVSTII
KNKIIPTTLEFMDHATLVCVERRFNLGLPDSGRAVLLIEVDGDRDLIEKQAGEIHKLIEPLGLVQFRVAKDAAESEELWR
VRRLVSPSLRDVNPDKFNEDIVVPRSKVPDVIRRIEVIQKRYDIPIVNFGHAGDGNIHVNIMIDKQVPGQLEKAHEAIKE
VFQAALDLGGTMSGEHGVGLAKQPYIPMELTADQVAAMKAIKRALDPNNILNPGKMLPV

Sequences:

>Translated_459_residues
MLDARILQQLTDIVGSENIAATPQDLICYGYDATQMEFLPDCVVHPDGSEEVARILKLANSEGFPVFPRGAGSGFTGGAL
PKGGGVVLVTTRMNRILRIDTDNLVAEVEPGVVTETFQMAVEKLGLFYPPDPASLKFSTLGGNVAECAGGPRAVKYGVTK
DFVMGLEVVLPTGEVIRTGGETVKGVVGYDLTKLICGSEGTLGVITKIIFKLLPYPDAKKTMLTVFDSIDGAARAVSTII
KNKIIPTTLEFMDHATLVCVERRFNLGLPDSGRAVLLIEVDGDRDLIEKQAGEIHKLIEPLGLVQFRVAKDAAESEELWR
VRRLVSPSLRDVNPDKFNEDIVVPRSKVPDVIRRIEVIQKRYDIPIVNFGHAGDGNIHVNIMIDKQVPGQLEKAHEAIKE
VFQAALDLGGTMSGEHGVGLAKQPYIPMELTADQVAAMKAIKRALDPNNILNPGKMLPV
>Mature_459_residues
MLDARILQQLTDIVGSENIAATPQDLICYGYDATQMEFLPDCVVHPDGSEEVARILKLANSEGFPVFPRGAGSGFTGGAL
PKGGGVVLVTTRMNRILRIDTDNLVAEVEPGVVTETFQMAVEKLGLFYPPDPASLKFSTLGGNVAECAGGPRAVKYGVTK
DFVMGLEVVLPTGEVIRTGGETVKGVVGYDLTKLICGSEGTLGVITKIIFKLLPYPDAKKTMLTVFDSIDGAARAVSTII
KNKIIPTTLEFMDHATLVCVERRFNLGLPDSGRAVLLIEVDGDRDLIEKQAGEIHKLIEPLGLVQFRVAKDAAESEELWR
VRRLVSPSLRDVNPDKFNEDIVVPRSKVPDVIRRIEVIQKRYDIPIVNFGHAGDGNIHVNIMIDKQVPGQLEKAHEAIKE
VFQAALDLGGTMSGEHGVGLAKQPYIPMELTADQVAAMKAIKRALDPNNILNPGKMLPV

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=428, Percent_Identity=32.2429906542056, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI37595754, Length=451, Percent_Identity=30.5986696230599, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI119964728, Length=427, Percent_Identity=31.3817330210773, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI4501993, Length=466, Percent_Identity=24.4635193133047, Blast_Score=125, Evalue=7e-29,
Organism=Escherichia coli, GI1789351, Length=454, Percent_Identity=36.1233480176211, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1787977, Length=528, Percent_Identity=25.3787878787879, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI48994907, Length=454, Percent_Identity=23.7885462555066, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994914, Length=175, Percent_Identity=34.8571428571429, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71992373, Length=466, Percent_Identity=31.3304721030043, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI71992381, Length=466, Percent_Identity=30.0429184549356, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17534361, Length=474, Percent_Identity=28.6919831223629, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI71992392, Length=384, Percent_Identity=31.25, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17556096, Length=463, Percent_Identity=25.4859611231102, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320027, Length=471, Percent_Identity=28.0254777070064, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6320023, Length=472, Percent_Identity=28.3898305084746, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6320764, Length=481, Percent_Identity=27.2349272349272, Blast_Score=162, Evalue=9e-41,
Organism=Drosophila melanogaster, GI18921117, Length=427, Percent_Identity=31.3817330210773, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24639277, Length=427, Percent_Identity=31.3817330210773, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24639275, Length=427, Percent_Identity=31.3817330210773, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24653753, Length=485, Percent_Identity=24.9484536082474, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 49708; Mature: 49708

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDARILQQLTDIVGSENIAATPQDLICYGYDATQMEFLPDCVVHPDGSEEVARILKLAN
CCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHCCCCEECCCCHHHHHHHHHHHC
SEGFPVFPRGAGSGFTGGALPKGGGVVLVTTRMNRILRIDTDNLVAEVEPGVVTETFQMA
CCCCEECCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCEEEECCCCCHHHHHHHH
VEKLGLFYPPDPASLKFSTLGGNVAECAGGPRAVKYGVTKDFVMGLEVVLPTGEVIRTGG
HHHCCCCCCCCCCCEEEEECCCCHHHHCCCCCEEEECCCHHHHHCCCEEECCCHHHHCCC
ETVKGVVGYDLTKLICGSEGTLGVITKIIFKLLPYPDAKKTMLTVFDSIDGAARAVSTII
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHH
KNKIIPTTLEFMDHATLVCVERRFNLGLPDSGRAVLLIEVDGDRDLIEKQAGEIHKLIEP
HCCCCHHHHHHHCCHHEEEEEHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
LGLVQFRVAKDAAESEELWRVRRLVSPSLRDVNPDKFNEDIVVPRSKVPDVIRRIEVIQK
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHH
RYDIPIVNFGHAGDGNIHVNIMIDKQVPGQLEKAHEAIKEVFQAALDLGGTMSGEHGVGL
HCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
AKQPYIPMELTADQVAAMKAIKRALDPNNILNPGKMLPV
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLDARILQQLTDIVGSENIAATPQDLICYGYDATQMEFLPDCVVHPDGSEEVARILKLAN
CCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHCCCCEECCCCHHHHHHHHHHHC
SEGFPVFPRGAGSGFTGGALPKGGGVVLVTTRMNRILRIDTDNLVAEVEPGVVTETFQMA
CCCCEECCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCEEEECCCCCHHHHHHHH
VEKLGLFYPPDPASLKFSTLGGNVAECAGGPRAVKYGVTKDFVMGLEVVLPTGEVIRTGG
HHHCCCCCCCCCCCEEEEECCCCHHHHCCCCCEEEECCCHHHHHCCCEEECCCHHHHCCC
ETVKGVVGYDLTKLICGSEGTLGVITKIIFKLLPYPDAKKTMLTVFDSIDGAARAVSTII
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHH
KNKIIPTTLEFMDHATLVCVERRFNLGLPDSGRAVLLIEVDGDRDLIEKQAGEIHKLIEP
HCCCCHHHHHHHCCHHEEEEEHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
LGLVQFRVAKDAAESEELWRVRRLVSPSLRDVNPDKFNEDIVVPRSKVPDVIRRIEVIQK
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHH
RYDIPIVNFGHAGDGNIHVNIMIDKQVPGQLEKAHEAIKEVFQAALDLGGTMSGEHGVGL
HCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
AKQPYIPMELTADQVAAMKAIKRALDPNNILNPGKMLPV
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]