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The map label for this gene is glcF-1 [H]

Identifier: 197116960

GI number: 197116960

Start: 680082

End: 681362

Strand: Reverse

Name: glcF-1 [H]

Synonym: Gbem_0563

Alternate gene names: 197116960

Gene position: 681362-680082 (Counterclockwise)

Preceding gene: 197116961

Following gene: 197116959

Centisome position: 14.76

GC content: 63.47

Gene sequence:

>1281_bases
ATGACCAAGAAGCAGATCGACCCGCTCGAGCGGGTGGCAGCAGAGATGAAAAAATGCGTTAAGTGCGGCGCCTGCCGCGC
ACACTGCCCCGCCTTCAGCACCTTTCAGCGCGAACCGGCTACGGCCCGCGGCAAGGTAGCCCTGGCGCAGCACCTTTGCA
AGGGGGACATAACCCTGGACGACGGCACCTACTCCGCCATGTCCAAGTGCCTTCTTTGCGGCAGCTGCGTGGAAAAGTGC
CCGAACCAAGTCCCGACCGACGAGATAGTGATCGCCGCACGCGAGGCGCTGGCGCAGCGGCGCGGGCTCACCACCTTTCA
TAAAGCGGTGGGGCAGGTGATCAAGAACCGCAAGGTGATGAACTTCGGCGCGCTCGCCGCCGCCATCCTCGGCCCTCTCT
TCTTCAGGAAGGTCCCGGCCACCTCGGGGCTCAGGCTCCGTTTCCCCATGCCGTTTATCGGGGGAAGCCGCCACATCCCG
CAGATCGCCAAAAAACCGTTTATGAAACGCCACCCCGAGGTAATCCAGGGGGAACCGGGCAAACCCCGTATCGTCTTCTT
CGTCGGCTGCATGACCAACTTCGTCTACACGGAGATCGGCGAGGCGACCCTTGCCCTCTTCCGTCACTTAGGCTGCACCG
TCATCATCCCCAAAGGGCAGCAGTGCTGCGGCCTGCCGGGGATGTCGGGGGGCGACCTGAACACGGTGCGCGAGCTGGCC
GAGATGAACCTTGCCGCCATCGAGGCGCACCAGGCTGACTACGTGATGACCGCCTGCGCCACCTGCGGCGGCGCTCTGCA
CAAGCTCTACCCGCTGGTGGTCGGCAAGCGCAACCCGGAACTGAAACAGCGGCTCCAGGCTCTGGCCGACAAGACGGTGG
ACGCGGCGGTGCTCTTGCAAAAACTCGGTCTCACCCCGGAAGAAACCGGGACCGGGTCCGGCATGCGCATCACCTACCAC
GACCCCTGCCACCACAGAACCGCCGGCATCACCAAGGAGCCGCGCGCCCTGCTCAAGAGAACGCCGGGACTGGAGCTGGT
GGAAATGGAAGGCGCGGACCGCTGTTGCGGCCTGGGCGGAACCTTCAACGTTTACCATTACGAAAATTCCCTCGACATCA
ACGCTAGCAAGAGCGCCGCGATCATCGCAACCGGCGCCGACGCGGTGGTGACCGGATGCCCCGGCTGCATCATGCAGCTC
TCCGACGGACTGAAACAGGCCGGAGATAAAACGAGGGTATTGCATACCGTGGAACTTCTGGCCCGCAAAATCAGGCGCTG
A

Upstream 100 bases:

>100_bases
GCAGAAGCCTTGCGCTGGCAGCAAGTCTGAAGTCGTTACTATAAAACCAGTTGTCAATTGTCCATTGTCAATTGTCAATT
GCCTTGAAGCGAGAACATAG

Downstream 100 bases:

>100_bases
CAGGCAACCCCTGTCAGCCGCCTCCCCCCTGCGTTTTCCCCTCAATTTTTCCCTGCCGCACAACCTCTTGGAATCCCTAC
ATGTCGGGTGTATACACGCT

Product: D-lactate/glycolate dehydrogenase iron-sulfur cluster-binding protein

Products: FADH2; dihydroxy-acetone-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTKKQIDPLERVAAEMKKCVKCGACRAHCPAFSTFQREPATARGKVALAQHLCKGDITLDDGTYSAMSKCLLCGSCVEKC
PNQVPTDEIVIAAREALAQRRGLTTFHKAVGQVIKNRKVMNFGALAAAILGPLFFRKVPATSGLRLRFPMPFIGGSRHIP
QIAKKPFMKRHPEVIQGEPGKPRIVFFVGCMTNFVYTEIGEATLALFRHLGCTVIIPKGQQCCGLPGMSGGDLNTVRELA
EMNLAAIEAHQADYVMTACATCGGALHKLYPLVVGKRNPELKQRLQALADKTVDAAVLLQKLGLTPEETGTGSGMRITYH
DPCHHRTAGITKEPRALLKRTPGLELVEMEGADRCCGLGGTFNVYHYENSLDINASKSAAIIATGADAVVTGCPGCIMQL
SDGLKQAGDKTRVLHTVELLARKIRR

Sequences:

>Translated_426_residues
MTKKQIDPLERVAAEMKKCVKCGACRAHCPAFSTFQREPATARGKVALAQHLCKGDITLDDGTYSAMSKCLLCGSCVEKC
PNQVPTDEIVIAAREALAQRRGLTTFHKAVGQVIKNRKVMNFGALAAAILGPLFFRKVPATSGLRLRFPMPFIGGSRHIP
QIAKKPFMKRHPEVIQGEPGKPRIVFFVGCMTNFVYTEIGEATLALFRHLGCTVIIPKGQQCCGLPGMSGGDLNTVRELA
EMNLAAIEAHQADYVMTACATCGGALHKLYPLVVGKRNPELKQRLQALADKTVDAAVLLQKLGLTPEETGTGSGMRITYH
DPCHHRTAGITKEPRALLKRTPGLELVEMEGADRCCGLGGTFNVYHYENSLDINASKSAAIIATGADAVVTGCPGCIMQL
SDGLKQAGDKTRVLHTVELLARKIRR
>Mature_425_residues
TKKQIDPLERVAAEMKKCVKCGACRAHCPAFSTFQREPATARGKVALAQHLCKGDITLDDGTYSAMSKCLLCGSCVEKCP
NQVPTDEIVIAAREALAQRRGLTTFHKAVGQVIKNRKVMNFGALAAAILGPLFFRKVPATSGLRLRFPMPFIGGSRHIPQ
IAKKPFMKRHPEVIQGEPGKPRIVFFVGCMTNFVYTEIGEATLALFRHLGCTVIIPKGQQCCGLPGMSGGDLNTVRELAE
MNLAAIEAHQADYVMTACATCGGALHKLYPLVVGKRNPELKQRLQALADKTVDAAVLLQKLGLTPEETGTGSGMRITYHD
PCHHRTAGITKEPRALLKRTPGLELVEMEGADRCCGLGGTFNVYHYENSLDINASKSAAIIATGADAVVTGCPGCIMQLS
DGLKQAGDKTRVLHTVELLARKIRR

Specific function: Electron Transfer Protein; May Also Function As The Membrane Anchor For The Glpab Dimer. [C]

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Loosely Bound To The Cytoplasm Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788576, Length=419, Percent_Identity=26.0143198090692, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786497, Length=243, Percent_Identity=29.2181069958848, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI48994913, Length=423, Percent_Identity=23.1678486997636, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 46033; Mature: 45901

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKQIDPLERVAAEMKKCVKCGACRAHCPAFSTFQREPATARGKVALAQHLCKGDITLD
CCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCEEC
DGTYSAMSKCLLCGSCVEKCPNQVPTDEIVIAAREALAQRRGLTTFHKAVGQVIKNRKVM
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
NFGALAAAILGPLFFRKVPATSGLRLRFPMPFIGGSRHIPQIAKKPFMKRHPEVIQGEPG
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHCCHHHCCCHHHCCCCC
KPRIVFFVGCMTNFVYTEIGEATLALFRHLGCTVIIPKGQQCCGLPGMSGGDLNTVRELA
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHH
EMNLAAIEAHQADYVMTACATCGGALHKLYPLVVGKRNPELKQRLQALADKTVDAAVLLQ
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
KLGLTPEETGTGSGMRITYHDPCHHRTAGITKEPRALLKRTPGLELVEMEGADRCCGLGG
HHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCC
TFNVYHYENSLDINASKSAAIIATGADAVVTGCPGCIMQLSDGLKQAGDKTRVLHTVELL
CEEEEEECCCCCCCCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
ARKIRR
HHHHCC
>Mature Secondary Structure 
TKKQIDPLERVAAEMKKCVKCGACRAHCPAFSTFQREPATARGKVALAQHLCKGDITLD
CCCCCCHHHHHHHHHHHHHHHCCCHHCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCEEC
DGTYSAMSKCLLCGSCVEKCPNQVPTDEIVIAAREALAQRRGLTTFHKAVGQVIKNRKVM
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
NFGALAAAILGPLFFRKVPATSGLRLRFPMPFIGGSRHIPQIAKKPFMKRHPEVIQGEPG
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHCCHHHCCCHHHCCCCC
KPRIVFFVGCMTNFVYTEIGEATLALFRHLGCTVIIPKGQQCCGLPGMSGGDLNTVRELA
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHH
EMNLAAIEAHQADYVMTACATCGGALHKLYPLVVGKRNPELKQRLQALADKTVDAAVLLQ
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
KLGLTPEETGTGSGMRITYHDPCHHRTAGITKEPRALLKRTPGLELVEMEGADRCCGLGG
HHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCC
TFNVYHYENSLDINASKSAAIIATGADAVVTGCPGCIMQLSDGLKQAGDKTRVLHTVELL
CEEEEEECCCCCCCCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
ARKIRR
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: FAD; glycerol-3-phosphate [C]

Specific reaction: FAD + glycerol-3-phosphate = FADH2 + dihydroxy-acetone-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]