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The map label for this gene is gacS [H]

Identifier: 197116920

GI number: 197116920

Start: 631686

End: 632063

Strand: Direct

Name: gacS [H]

Synonym: Gbem_0522

Alternate gene names: 197116920

Gene position: 631686-632063 (Clockwise)

Preceding gene: 197116917

Following gene: 308535171

Centisome position: 13.69

GC content: 52.91

Gene sequence:

>378_bases
ATGCGGATTTTAGTAGCAGAAGACGACCCAACCATCAGGCAGATGATGGCGACGCTGCTGTCACGGTGCGGTTTTGACTG
CCGCAGCGTCGATAATGGCCGCAAAGCCGTGGAGGCGTGGGAAGATGAAACCTTTGATTTCATCTTCATGGACGTACAAA
TGCCGGTCATGGACGGTTTCGCCGCTACCAGGATGATCCGCGAAAAGGAAGCCCTCAAAGGAGGTCACACTCCGATAATC
GCCCTGACCGCTCACGCAATGGAGCAGGACAGGCAACAATGCCTCGACTCCGGCATGGACGATTACCTCTCTAAGCCAAT
CGACCTCGACGAACTGTTCTCTCTTTTAAAAAACGACTACTCAAAAGGCGTCAGCTAA

Upstream 100 bases:

>100_bases
TCATATCTTGCTCACCAGTCTTTACTTTGGATAACCGGATGCTATCTTGATACCGGCTTGTTATTGCAGTCGTTGCACCT
GTGAGCACAGGAGTAAGCCA

Downstream 100 bases:

>100_bases
ACCTTACCTCCCCCCCCCTTACAAGGGACTCCATGATGCCGGAAAGCCGAAAGGACATGGGCGATCAGAAGCACAAGCAG
TATTACGCGCTCTACAAGGA

Product: response receiver

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MRILVAEDDPTIRQMMATLLSRCGFDCRSVDNGRKAVEAWEDETFDFIFMDVQMPVMDGFAATRMIREKEALKGGHTPII
ALTAHAMEQDRQQCLDSGMDDYLSKPIDLDELFSLLKNDYSKGVS

Sequences:

>Translated_125_residues
MRILVAEDDPTIRQMMATLLSRCGFDCRSVDNGRKAVEAWEDETFDFIFMDVQMPVMDGFAATRMIREKEALKGGHTPII
ALTAHAMEQDRQQCLDSGMDDYLSKPIDLDELFSLLKNDYSKGVS
>Mature_125_residues
MRILVAEDDPTIRQMMATLLSRCGFDCRSVDNGRKAVEAWEDETFDFIFMDVQMPVMDGFAATRMIREKEALKGGHTPII
ALTAHAMEQDRQQCLDSGMDDYLSKPIDLDELFSLLKNDYSKGVS

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=110, Percent_Identity=39.0909090909091, Blast_Score=90, Evalue=5e-20,
Organism=Escherichia coli, GI1789149, Length=116, Percent_Identity=37.9310344827586, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI1788550, Length=111, Percent_Identity=37.8378378378378, Blast_Score=82, Evalue=1e-17,
Organism=Escherichia coli, GI1790299, Length=119, Percent_Identity=31.9327731092437, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1788713, Length=112, Percent_Identity=36.6071428571429, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1789402, Length=117, Percent_Identity=37.6068376068376, Blast_Score=68, Evalue=2e-13,
Organism=Escherichia coli, GI1789809, Length=116, Percent_Identity=32.7586206896552, Blast_Score=67, Evalue=3e-13,
Organism=Escherichia coli, GI87082012, Length=117, Percent_Identity=31.6239316239316, Blast_Score=67, Evalue=3e-13,
Organism=Escherichia coli, GI1790437, Length=115, Percent_Identity=33.0434782608696, Blast_Score=66, Evalue=9e-13,
Organism=Escherichia coli, GI1790552, Length=129, Percent_Identity=32.5581395348837, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1786599, Length=116, Percent_Identity=31.0344827586207, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1787229, Length=116, Percent_Identity=28.448275862069, Blast_Score=64, Evalue=2e-12,
Organism=Escherichia coli, GI1786784, Length=125, Percent_Identity=30.4, Blast_Score=64, Evalue=3e-12,
Organism=Escherichia coli, GI48994928, Length=118, Percent_Identity=28.8135593220339, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1786911, Length=115, Percent_Identity=31.304347826087, Blast_Score=60, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=114, Percent_Identity=35.9649122807018, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322044, Length=122, Percent_Identity=36.0655737704918, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323034, Length=133, Percent_Identity=27.8195488721804, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 14171; Mature: 14171

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
7.2 %Met     (Translated Protein)
9.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
7.2 %Met     (Mature Protein)
9.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVAEDDPTIRQMMATLLSRCGFDCRSVDNGRKAVEAWEDETFDFIFMDVQMPVMDGF
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHH
AATRMIREKEALKGGHTPIIALTAHAMEQDRQQCLDSGMDDYLSKPIDLDELFSLLKNDY
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHH
SKGVS
HCCCC
>Mature Secondary Structure
MRILVAEDDPTIRQMMATLLSRCGFDCRSVDNGRKAVEAWEDETFDFIFMDVQMPVMDGF
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHH
AATRMIREKEALKGGHTPIIALTAHAMEQDRQQCLDSGMDDYLSKPIDLDELFSLLKNDY
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHH
SKGVS
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]