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The map label for this gene is mscL-1 [H]

Identifier: 197116785

GI number: 197116785

Start: 464384

End: 464818

Strand: Direct

Name: mscL-1 [H]

Synonym: Gbem_0387

Alternate gene names: 197116785

Gene position: 464384-464818 (Clockwise)

Preceding gene: 197116782

Following gene: 197116786

Centisome position: 10.06

GC content: 54.94

Gene sequence:

>435_bases
ATGCTTAAAGAATTCAAGCAGTTTGCTCTCAAGGGGAACGTGCTGGACTTGGCTGTAGCTGTCGTGCTCGGCGCCGCGTT
CGGAAAAATTGTAAGCTCCTTCGTATCCGACATACTCACCCCGCCCATCGGAAAACTCCTCGGAAACATCGACTTCTCCT
CGTTATTCCTGAATCTCACCAGCACCCCGATCCGCTCTGTAGCAGAGGCTAAAGCCCTGGGGGCGCCGGTCATCGCCTAT
GGTCTCTTCCTGAACGCTGTATTTGACTTCATCATCATCGCCTTTGCGCTGTTCCTGCTGGTGCGCCAGGTGAGCAAGAT
GCTTCCCCCTCCGACACCCCCGGTTGCCACCAAGGAATGTCCACACTGCTGCACCATGATACCTGTCAAAGCGTCCCGTT
GTCCCAACTGCACTTCATCCCAGTCTGAAGCCTAA

Upstream 100 bases:

>100_bases
AAGGGAAAATAAACCATTTTTCTTGCGCCCGATGTAAAGGGTGGTAGCCTTTCCCATGTGTTTTTTTGCTGAGGTCATCT
CATAAACGGGGGGGTAACGG

Downstream 100 bases:

>100_bases
CTACGATAGGAGGTGTGAGATGAAAAAGTACCTGCAGCTTGTTCTGGTTCCTGCGTTGCTTTTGGGCCTGTTCACGCAGT
CGTACGCAGAGGTGAAGCCG

Product: large-conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MLKEFKQFALKGNVLDLAVAVVLGAAFGKIVSSFVSDILTPPIGKLLGNIDFSSLFLNLTSTPIRSVAEAKALGAPVIAY
GLFLNAVFDFIIIAFALFLLVRQVSKMLPPPTPPVATKECPHCCTMIPVKASRCPNCTSSQSEA

Sequences:

>Translated_144_residues
MLKEFKQFALKGNVLDLAVAVVLGAAFGKIVSSFVSDILTPPIGKLLGNIDFSSLFLNLTSTPIRSVAEAKALGAPVIAY
GLFLNAVFDFIIIAFALFLLVRQVSKMLPPPTPPVATKECPHCCTMIPVKASRCPNCTSSQSEA
>Mature_144_residues
MLKEFKQFALKGNVLDLAVAVVLGAAFGKIVSSFVSDILTPPIGKLLGNIDFSSLFLNLTSTPIRSVAEAKALGAPVIAY
GLFLNAVFDFIIIAFALFLLVRQVSKMLPPPTPPVATKECPHCCTMIPVKASRCPNCTSSQSEA

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=123, Percent_Identity=44.7154471544715, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 15312; Mature: 15312

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEFKQFALKGNVLDLAVAVVLGAAFGKIVSSFVSDILTPPIGKLLGNIDFSSLFLNLT
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHH
STPIRSVAEAKALGAPVIAYGLFLNAVFDFIIIAFALFLLVRQVSKMLPPPTPPVATKEC
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PHCCTMIPVKASRCPNCTSSQSEA
CCHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKEFKQFALKGNVLDLAVAVVLGAAFGKIVSSFVSDILTPPIGKLLGNIDFSSLFLNLT
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHH
STPIRSVAEAKALGAPVIAYGLFLNAVFDFIIIAFALFLLVRQVSKMLPPPTPPVATKEC
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PHCCTMIPVKASRCPNCTSSQSEA
CCHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA