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The map label for this gene is oprF [H]

Identifier: 197116786

GI number: 197116786

Start: 464838

End: 466217

Strand: Direct

Name: oprF [H]

Synonym: Gbem_0388

Alternate gene names: 197116786

Gene position: 464838-466217 (Clockwise)

Preceding gene: 197116785

Following gene: 197116793

Centisome position: 10.07

GC content: 59.28

Gene sequence:

>1380_bases
ATGAAAAAGTACCTGCAGCTTGTTCTGGTTCCTGCGTTGCTTTTGGGCCTGTTCACGCAGTCGTACGCAGAGGTGAAGCC
GGAAAGCTTCTCCGTTTCTCCCTACGTTGCCGGTTACAGCTTTTTTGGGAGGGAACGGCTTGAAACCGGACCAGCGGTCG
GATTGCGTGGTGGCTACAACTTCACCAGCCATTTCGGCATGGAGGCGGTGCTGACCTACATAAGCACCGAAGGGAAGGAC
GGTTCCGGGATCGGGGACGTGGACGCTCTCAACTACCACCTGGACATGCTGTATCACTTCATGCCCGAAAATACGCTGGT
CCCTTACCTCGCCATGGGGTATGGCGGCCACTGGCGCGATTACGAGGGGGATGGGGAAGTGAACCGATCCGCTTTTAACT
ATGGCGGCGGGGTGAAGTATTTTCTTACCGATGCCATGGCGCTTAGAGGCGATGTGCGTCATATCATAATGAAGGACCGG
GATGAAACCTTCCACGACCTTGAATACGGCCTGGGGGTCGATTTCATTTTCGGCGGGGCCAAGGCCGCTCCGGCAGTTGC
CGCCGCCCCCGTGGCCGCTCCCGTGGTGCAACCTCCGGCAGCGGAAGCCCCCTTGGAGCCGGTACCGGCAGCGGAACCTG
CGCCGGGGCACTACAAGTATTGTGTAACACTGCAGGGCGAGTTCGACATAGACAAAGCCGAGATCAGGCCTGAATACCGC
GACGAGGTCGCAACAGTCGGCAAGTTCATGAAGCAGTACCCGACCACCACCGCGGTTATCGAAGGGCACACCGATAACGT
CGGCGATCCCCAGTACAACCTCGATCTTTCCAAGCGCCGCGCGCAGGCAGTCGTCGATTATCTGGTCGACAACTACGGTA
TCGAGCGCTCGCGCCTGGAGGCCCGGGGCTTTGGTATGGCGCGTCCGATAGCCACCAACAACACCGACGAGGGGAGGCAG
GCCAACCGCCGCATCGAGGCGATCATCGACTGCGCCTTCGACGTCAAGGAAGTCCAGCCCCCGGACCGGCTCTGCATGAC
CCTGCTGCTCGAGTTCGATAGCGGCAAAGCCGATATCAAGCCGCAGTATCGCGACGAAATGGCCAAAGTAGGGGAATACA
TGAACAAGTATCCCACCACGACGGCGGTTATCGAAGGCCACACCGACAACGTCGGCGGCTCCGACTACAACATGAAGTTG
TCGCAGCGACGCGCGGAAAACGCGGTGCAGTACCTGGTGCAGAATTTCGGCATAGATAAAAGCCGCCTCTCCGCCAAGGG
GTACGGCTACACGCGGCGCATCGCCTACAACAGCACGCCCGAGGGGCGAGCCAAGAACCGCAGGATCAACGCCGTCATCG
ACTGCGTCATCAAGAAATAG

Upstream 100 bases:

>100_bases
GAATGTCCACACTGCTGCACCATGATACCTGTCAAAGCGTCCCGTTGTCCCAACTGCACTTCATCCCAGTCTGAAGCCTA
ACTACGATAGGAGGTGTGAG

Downstream 100 bases:

>100_bases
CTAAAATCTAGATTAAGACTAAGACTAAGATTAAGATTGAGTCTAAGGCAAAGACTTGAGAAGAACTAAAGAAGGGGAAG
CCGTTTTGGCTTCCCCTTCC

Product: OmpA family outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MKKYLQLVLVPALLLGLFTQSYAEVKPESFSVSPYVAGYSFFGRERLETGPAVGLRGGYNFTSHFGMEAVLTYISTEGKD
GSGIGDVDALNYHLDMLYHFMPENTLVPYLAMGYGGHWRDYEGDGEVNRSAFNYGGGVKYFLTDAMALRGDVRHIIMKDR
DETFHDLEYGLGVDFIFGGAKAAPAVAAAPVAAPVVQPPAAEAPLEPVPAAEPAPGHYKYCVTLQGEFDIDKAEIRPEYR
DEVATVGKFMKQYPTTTAVIEGHTDNVGDPQYNLDLSKRRAQAVVDYLVDNYGIERSRLEARGFGMARPIATNNTDEGRQ
ANRRIEAIIDCAFDVKEVQPPDRLCMTLLLEFDSGKADIKPQYRDEMAKVGEYMNKYPTTTAVIEGHTDNVGGSDYNMKL
SQRRAENAVQYLVQNFGIDKSRLSAKGYGYTRRIAYNSTPEGRAKNRRINAVIDCVIKK

Sequences:

>Translated_459_residues
MKKYLQLVLVPALLLGLFTQSYAEVKPESFSVSPYVAGYSFFGRERLETGPAVGLRGGYNFTSHFGMEAVLTYISTEGKD
GSGIGDVDALNYHLDMLYHFMPENTLVPYLAMGYGGHWRDYEGDGEVNRSAFNYGGGVKYFLTDAMALRGDVRHIIMKDR
DETFHDLEYGLGVDFIFGGAKAAPAVAAAPVAAPVVQPPAAEAPLEPVPAAEPAPGHYKYCVTLQGEFDIDKAEIRPEYR
DEVATVGKFMKQYPTTTAVIEGHTDNVGDPQYNLDLSKRRAQAVVDYLVDNYGIERSRLEARGFGMARPIATNNTDEGRQ
ANRRIEAIIDCAFDVKEVQPPDRLCMTLLLEFDSGKADIKPQYRDEMAKVGEYMNKYPTTTAVIEGHTDNVGGSDYNMKL
SQRRAENAVQYLVQNFGIDKSRLSAKGYGYTRRIAYNSTPEGRAKNRRINAVIDCVIKK
>Mature_459_residues
MKKYLQLVLVPALLLGLFTQSYAEVKPESFSVSPYVAGYSFFGRERLETGPAVGLRGGYNFTSHFGMEAVLTYISTEGKD
GSGIGDVDALNYHLDMLYHFMPENTLVPYLAMGYGGHWRDYEGDGEVNRSAFNYGGGVKYFLTDAMALRGDVRHIIMKDR
DETFHDLEYGLGVDFIFGGAKAAPAVAAAPVAAPVVQPPAAEAPLEPVPAAEPAPGHYKYCVTLQGEFDIDKAEIRPEYR
DEVATVGKFMKQYPTTTAVIEGHTDNVGDPQYNLDLSKRRAQAVVDYLVDNYGIERSRLEARGFGMARPIATNNTDEGRQ
ANRRIEAIIDCAFDVKEVQPPDRLCMTLLLEFDSGKADIKPQYRDEMAKVGEYMNKYPTTTAVIEGHTDNVGGSDYNMKL
SQRRAENAVQYLVQNFGIDKSRLSAKGYGYTRRIAYNSTPEGRAKNRRINAVIDCVIKK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=100, Percent_Identity=41, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788957, Length=96, Percent_Identity=37.5, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787191, Length=109, Percent_Identity=35.7798165137615, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 50930; Mature: 50930

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYLQLVLVPALLLGLFTQSYAEVKPESFSVSPYVAGYSFFGRERLETGPAVGLRGGYN
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCHHHHHHHHHCCCCCEECCCCCC
FTSHFGMEAVLTYISTEGKDGSGIGDVDALNYHLDMLYHFMPENTLVPYLAMGYGGHWRD
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHEECCCCCCCC
YEGDGEVNRSAFNYGGGVKYFLTDAMALRGDVRHIIMKDRDETFHDLEYGLGVDFIFGGA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHCCCEEEEECCC
KAAPAVAAAPVAAPVVQPPAAEAPLEPVPAAEPAPGHYKYCVTLQGEFDIDKAEIRPEYR
CCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHCCCHHH
DEVATVGKFMKQYPTTTAVIEGHTDNVGDPQYNLDLSKRRAQAVVDYLVDNYGIERSRLE
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
ARGFGMARPIATNNTDEGRQANRRIEAIIDCAFDVKEVQPPDRLCMTLLLEFDSGKADIK
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
PQYRDEMAKVGEYMNKYPTTTAVIEGHTDNVGGSDYNMKLSQRRAENAVQYLVQNFGIDK
CHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCH
SRLSAKGYGYTRRIAYNSTPEGRAKNRRINAVIDCVIKK
HHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHEEEECC
>Mature Secondary Structure
MKKYLQLVLVPALLLGLFTQSYAEVKPESFSVSPYVAGYSFFGRERLETGPAVGLRGGYN
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCHHHHHHHHHCCCCCEECCCCCC
FTSHFGMEAVLTYISTEGKDGSGIGDVDALNYHLDMLYHFMPENTLVPYLAMGYGGHWRD
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHEECCCCCCCC
YEGDGEVNRSAFNYGGGVKYFLTDAMALRGDVRHIIMKDRDETFHDLEYGLGVDFIFGGA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHCCCEEEEECCC
KAAPAVAAAPVAAPVVQPPAAEAPLEPVPAAEPAPGHYKYCVTLQGEFDIDKAEIRPEYR
CCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHCCCHHH
DEVATVGKFMKQYPTTTAVIEGHTDNVGDPQYNLDLSKRRAQAVVDYLVDNYGIERSRLE
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
ARGFGMARPIATNNTDEGRQANRRIEAIIDCAFDVKEVQPPDRLCMTLLLEFDSGKADIK
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
PQYRDEMAKVGEYMNKYPTTTAVIEGHTDNVGGSDYNMKLSQRRAENAVQYLVQNFGIDK
CHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCH
SRLSAKGYGYTRRIAYNSTPEGRAKNRRINAVIDCVIKK
HHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]