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The map label for this gene is pulE-2 [H]

Identifier: 197116768

GI number: 197116768

Start: 445783

End: 447699

Strand: Reverse

Name: pulE-2 [H]

Synonym: Gbem_0368

Alternate gene names: 197116768

Gene position: 447699-445783 (Counterclockwise)

Preceding gene: 197116771

Following gene: 197116767

Centisome position: 9.7

GC content: 62.6

Gene sequence:

>1917_bases
ATGGAGAGTCACGGCAACTATAAATTAGAGGCGGATCGCAAGGAAGGGATGGACGACTCCGGCCTCGAGATCGCGGCCCT
GCTGATGAAGTCGGGTTACCTCGCGGACACCCAGCTCAGTTACGCGCAGCGGGTCAAGGCAAAACTCCTCTCCCCGAGAA
CGCTGGTCGGCGTGCTGCTGGAGCTCGGCTTCTTCACCCGGGAGCAGCTCAGGGAGACGCTCCGGAGCAACATGGTATCG
GTGAAGCTCGGGGCCCTGCTGGTGGAACTCGGCTACCTGAAACCGGGAGAGTTGCAGGCCGCCCTCGGGATACAGCGGGA
CGGGGAAAACTGCAAGATGCTGGGCGAGATCCTGGTGGAACAGCGCTTTATCGAGGAGTACACCCTGGCGGAGGTGCTCG
CCTTCCAACTGGGGTTCCCTTTCATCGACCTGGACGCCTCCAGCATCGACCGGGCGCTTTTGGGCCGGGTCCCCCAGCAC
TGGCTGGCGCAGCACAGCTTCGTTCCCGTCAAGGAGGAGGAGGGGAAGGTCCTGGTGGCGATCGCGGACCCCCTGAACCT
GGAGGGGAGAAAGACGGCGGAGAAGCTGTTCGGCCAGAACACCACCTCCTTCGCCATCTGCACCATGAAGGCGATCCGGG
ACGCGCTCAGCCTCTTGAAGCGGGGGATGGTGCAGGGGGACGGCACGGCGACCGACGAGCACACGGTGACCGGCATTGTG
AATTCCATCTTCGAAGAGGCCCTGAAGGAGGGGGCAAGCGACATACATATCGAGCCGATGCGCAGCGCGCTCAGGGTGCG
CTTCAGGCGGGACGGGATGCTGGTCCTATACAAGGACTTCGCCAGGGAGCTGGCGCTTCCCATCAGCAGCAGGATCAAGG
TTATGGCCGAGGCGGACATCGCCGAGAGGAGGAGGCACCAGGACGGCCGGATCTGCTACGAAAGCGCAAAGAGCGGCAAC
ACTCTGGACATGAGGGTCTCCTTCTACATCACCATCCACGGAGAGAAGATCGTGCTCCGCCTTCTGAGTATGAAAGCGGA
ACTCCTCGATCTCAAGGAGATCGGCATGTCCGGGCGCATGCTGGAGCGCTTCATCGACGACGCGCTGGATACGCCAAGCG
GCGTCCTGATCGTCACCGGGCCTACCGGCAGCGGCAAGACCTCCACGCTGTACAGTTGCGTGCACCACCTAAACGACCTG
AACACCTCCATCGTCACCGCCGAGGAACCGGTTGAATACGTGATTGAGGGGATCGCCCAGTGCTCCATCAATCAGAAGAT
CGGGGTGACCTTCGAGGAAACCCTCAGGCACATCGTGCGCCAGGACCCCGACGTGATCGTCCTGGGAGAAATCCGGGACA
GCTTCTCCGCCGAAACCGCGATCCAGGCGGCGCTCACCGGGCACAAGGTCCTCACCACCTTTCACACCGAGGACAGCGTC
GGGGGGCTCCTGCGCCTGATGAACATGAACATCGAGGCATTCCTGATCGCCTCCACCGTGGTCTGCGTCCTGGCGCAGCG
GCTTTTGCGCCGCATCTGTCCTGAGTGCAGCGAGAGCTACCTTCCCACGCCGACCGAGCTCCGGCGGATAGGCTACAGCA
ACGCCGACCTGCGGGGGGCCGAGTTCAGGATCGGACGGGGGTGCGCCAAATGCAGGTACAGCGGGTACCGCGGCAGGGTT
GGCGTATTCGAGATGCTGATACTGAACGAACTGGTGAAGGACGCCATCCTCAGCAAGAAGACCTCCTACGAGATCAGGAA
GATCTCCACGGAGAGCACCGGAATGGTCACGCTCCTGGAGTCGGGGCTTTGCAAGGCAGCCGCGGGGCTGGTGTCGCTGC
ACGACACGGTGCGGCTCCTCCCCAGGATCGGCAAACCGCGCCCGATTTCCGAGATCAGGCGCCTTTTGGGGGATTGA

Upstream 100 bases:

>100_bases
GGTATACTACCAAGGAATCGGGACCCATGTCAATGAAGAGTGGCTGCGATCGGAACCAATGTAAAGAGAGGGCGATCAGC
ACGGGCGCAGGAGATGGTGT

Downstream 100 bases:

>100_bases
CATGCACAGCAGACCCTTTGTCGAGGTAGTAAAGGAGCAGCTAGAATCAGAATCGCTCAACCTGCCGGTGTTCCACCCCA
TCGCCCTAAAGTTGCAGGGG

Product: type II secretion system ATPase PulE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MESHGNYKLEADRKEGMDDSGLEIAALLMKSGYLADTQLSYAQRVKAKLLSPRTLVGVLLELGFFTREQLRETLRSNMVS
VKLGALLVELGYLKPGELQAALGIQRDGENCKMLGEILVEQRFIEEYTLAEVLAFQLGFPFIDLDASSIDRALLGRVPQH
WLAQHSFVPVKEEEGKVLVAIADPLNLEGRKTAEKLFGQNTTSFAICTMKAIRDALSLLKRGMVQGDGTATDEHTVTGIV
NSIFEEALKEGASDIHIEPMRSALRVRFRRDGMLVLYKDFARELALPISSRIKVMAEADIAERRRHQDGRICYESAKSGN
TLDMRVSFYITIHGEKIVLRLLSMKAELLDLKEIGMSGRMLERFIDDALDTPSGVLIVTGPTGSGKTSTLYSCVHHLNDL
NTSIVTAEEPVEYVIEGIAQCSINQKIGVTFEETLRHIVRQDPDVIVLGEIRDSFSAETAIQAALTGHKVLTTFHTEDSV
GGLLRLMNMNIEAFLIASTVVCVLAQRLLRRICPECSESYLPTPTELRRIGYSNADLRGAEFRIGRGCAKCRYSGYRGRV
GVFEMLILNELVKDAILSKKTSYEIRKISTESTGMVTLLESGLCKAAAGLVSLHDTVRLLPRIGKPRPISEIRRLLGD

Sequences:

>Translated_638_residues
MESHGNYKLEADRKEGMDDSGLEIAALLMKSGYLADTQLSYAQRVKAKLLSPRTLVGVLLELGFFTREQLRETLRSNMVS
VKLGALLVELGYLKPGELQAALGIQRDGENCKMLGEILVEQRFIEEYTLAEVLAFQLGFPFIDLDASSIDRALLGRVPQH
WLAQHSFVPVKEEEGKVLVAIADPLNLEGRKTAEKLFGQNTTSFAICTMKAIRDALSLLKRGMVQGDGTATDEHTVTGIV
NSIFEEALKEGASDIHIEPMRSALRVRFRRDGMLVLYKDFARELALPISSRIKVMAEADIAERRRHQDGRICYESAKSGN
TLDMRVSFYITIHGEKIVLRLLSMKAELLDLKEIGMSGRMLERFIDDALDTPSGVLIVTGPTGSGKTSTLYSCVHHLNDL
NTSIVTAEEPVEYVIEGIAQCSINQKIGVTFEETLRHIVRQDPDVIVLGEIRDSFSAETAIQAALTGHKVLTTFHTEDSV
GGLLRLMNMNIEAFLIASTVVCVLAQRLLRRICPECSESYLPTPTELRRIGYSNADLRGAEFRIGRGCAKCRYSGYRGRV
GVFEMLILNELVKDAILSKKTSYEIRKISTESTGMVTLLESGLCKAAAGLVSLHDTVRLLPRIGKPRPISEIRRLLGD
>Mature_638_residues
MESHGNYKLEADRKEGMDDSGLEIAALLMKSGYLADTQLSYAQRVKAKLLSPRTLVGVLLELGFFTREQLRETLRSNMVS
VKLGALLVELGYLKPGELQAALGIQRDGENCKMLGEILVEQRFIEEYTLAEVLAFQLGFPFIDLDASSIDRALLGRVPQH
WLAQHSFVPVKEEEGKVLVAIADPLNLEGRKTAEKLFGQNTTSFAICTMKAIRDALSLLKRGMVQGDGTATDEHTVTGIV
NSIFEEALKEGASDIHIEPMRSALRVRFRRDGMLVLYKDFARELALPISSRIKVMAEADIAERRRHQDGRICYESAKSGN
TLDMRVSFYITIHGEKIVLRLLSMKAELLDLKEIGMSGRMLERFIDDALDTPSGVLIVTGPTGSGKTSTLYSCVHHLNDL
NTSIVTAEEPVEYVIEGIAQCSINQKIGVTFEETLRHIVRQDPDVIVLGEIRDSFSAETAIQAALTGHKVLTTFHTEDSV
GGLLRLMNMNIEAFLIASTVVCVLAQRLLRRICPECSESYLPTPTELRRIGYSNADLRGAEFRIGRGCAKCRYSGYRGRV
GVFEMLILNELVKDAILSKKTSYEIRKISTESTGMVTLLESGLCKAAAGLVSLHDTVRLLPRIGKPRPISEIRRLLGD

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=386, Percent_Identity=39.3782383419689, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1786296, Length=387, Percent_Identity=37.984496124031, Blast_Score=266, Evalue=4e-72,
Organism=Escherichia coli, GI87082188, Length=170, Percent_Identity=38.8235294117647, Blast_Score=99, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 70721; Mature: 70721

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESHGNYKLEADRKEGMDDSGLEIAALLMKSGYLADTQLSYAQRVKAKLLSPRTLVGVLL
CCCCCCEEEECHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
ELGFFTREQLRETLRSNMVSVKLGALLVELGYLKPGELQAALGIQRDGENCKMLGEILVE
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHH
QRFIEEYTLAEVLAFQLGFPFIDLDASSIDRALLGRVPQHWLAQHSFVPVKEEEGKVLVA
HHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCEEEE
IADPLNLEGRKTAEKLFGQNTTSFAICTMKAIRDALSLLKRGMVQGDGTATDEHTVTGIV
EECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
NSIFEEALKEGASDIHIEPMRSALRVRFRRDGMLVLYKDFARELALPISSRIKVMAEADI
HHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCHHHHHHEEECCCH
AERRRHQDGRICYESAKSGNTLDMRVSFYITIHGEKIVLRLLSMKAELLDLKEIGMSGRM
HHHHCCCCCCEEHHCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHH
LERFIDDALDTPSGVLIVTGPTGSGKTSTLYSCVHHLNDLNTSIVTAEEPVEYVIEGIAQ
HHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHH
CSINQKIGVTFEETLRHIVRQDPDVIVLGEIRDSFSAETAIQAALTGHKVLTTFHTEDSV
HCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCHH
GGLLRLMNMNIEAFLIASTVVCVLAQRLLRRICPECSESYLPTPTELRRIGYSNADLRGA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHCCCCCCCCCCC
EFRIGRGCAKCRYSGYRGRVGVFEMLILNELVKDAILSKKTSYEIRKISTESTGMVTLLE
CEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHEEECCCCCCHHHHHH
SGLCKAAAGLVSLHDTVRLLPRIGKPRPISEIRRLLGD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MESHGNYKLEADRKEGMDDSGLEIAALLMKSGYLADTQLSYAQRVKAKLLSPRTLVGVLL
CCCCCCEEEECHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
ELGFFTREQLRETLRSNMVSVKLGALLVELGYLKPGELQAALGIQRDGENCKMLGEILVE
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHH
QRFIEEYTLAEVLAFQLGFPFIDLDASSIDRALLGRVPQHWLAQHSFVPVKEEEGKVLVA
HHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCEEEE
IADPLNLEGRKTAEKLFGQNTTSFAICTMKAIRDALSLLKRGMVQGDGTATDEHTVTGIV
EECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
NSIFEEALKEGASDIHIEPMRSALRVRFRRDGMLVLYKDFARELALPISSRIKVMAEADI
HHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCHHHHHHEEECCCH
AERRRHQDGRICYESAKSGNTLDMRVSFYITIHGEKIVLRLLSMKAELLDLKEIGMSGRM
HHHHCCCCCCEEHHCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHH
LERFIDDALDTPSGVLIVTGPTGSGKTSTLYSCVHHLNDLNTSIVTAEEPVEYVIEGIAQ
HHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHH
CSINQKIGVTFEETLRHIVRQDPDVIVLGEIRDSFSAETAIQAALTGHKVLTTFHTEDSV
HCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCHH
GGLLRLMNMNIEAFLIASTVVCVLAQRLLRRICPECSESYLPTPTELRRIGYSNADLRGA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHCCCCCCCCCCC
EFRIGRGCAKCRYSGYRGRVGVFEMLILNELVKDAILSKKTSYEIRKISTESTGMVTLLE
CEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHEEECCCCCCHHHHHH
SGLCKAAAGLVSLHDTVRLLPRIGKPRPISEIRRLLGD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]