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The map label for this gene is nfo

Identifier: 197116756

GI number: 197116756

Start: 436156

End: 437007

Strand: Reverse

Name: nfo

Synonym: Gbem_0356

Alternate gene names: 197116756

Gene position: 437007-436156 (Counterclockwise)

Preceding gene: 197116757

Following gene: 197116755

Centisome position: 9.47

GC content: 63.62

Gene sequence:

>852_bases
ATGGACCTGCTGGGCGCACACGTCTCCATCGCCGGAGGCATACATAACGCTGTTGACCGCGGGGTAAGCTCCGGCTGCGG
GGTGATCCAGATCTTTACCCAGAACTCGAACCAGTGGAAAGGAAAGGCGGTTTCCCCGGCCGACGCCCAGCTCTTCCGGG
ACAAACTGACCGCCTCCGGGCTCTCTCACGTCGTCAGCCACGACATCTACCTGATAAACCTGGCAGCCGCCCCCGGCGAG
GTGAAGGACAAGAGCCTCATCGCCTTCAAGGAGGAGATGCAGCGCTGCGCAGCACTCGGCATCGACAAGATCGTCATGCA
TCCCGGCTCCCACACGGGCGACGGCGAGGAGACCGGCATCCGGCGCATCTGCGAGGCGTTCGACCAGCTCTTCGCCGAGG
TGCCGGAGTTCACCGGGAAGGTGCTGCTGGAAAACACCGCCGGCCAGGGGACGAACCTCGGCTACCGCTTCGATCACCTG
AAGTCGATCATAGAGGGGAGTTCCTATCCCACCCGCTTCGGCGTCTGCTTCGACACCTGCCACGCCTTCGCCTCGGGGTA
CCCCATCGCAGACCGGGACGGCTATCGCCGGACCTTCGACGAGTTCGACAGCGCGCTCGGGATCGACAAGCTGATGGCCT
TCCACCTGAACGACTCCAAGAAGGGGCTCGGCTGCAAGGTGGACCGCCACGAGCACATCGGCGCGGGGGCGCTCGGGCTG
GAACCCTTCCGCTTCATCCTGAACGACCCGCACTTCAAGCTGGTGCCGAAGTTCATCGAGACGCCCAAGGGGGACGCCGA
CGAGATGGATGTGGTGAACCTGAAACTCTTGAGGAGCCTGGTCGAAGGGTGA

Upstream 100 bases:

>100_bases
GGCCGCGCTGATCAAGGCACAGTTGAAGAACATCCTGGGCTAGCGCCTGCAACACGTCTCTCCCCCTTCACCGAAGGGGC
CAAAAGCCAAAAGGAAAACC

Downstream 100 bases:

>100_bases
CCGGGCGGGGGGTGCCTTCATGAGCGCTACGAAGCTCAGCGAAAGGGTGGAGATCATCCGCGGGGACATAACGAGGATAG
CCGTCGACGCCATCGTCAAC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MDLLGAHVSIAGGIHNAVDRGVSSGCGVIQIFTQNSNQWKGKAVSPADAQLFRDKLTASGLSHVVSHDIYLINLAAAPGE
VKDKSLIAFKEEMQRCAALGIDKIVMHPGSHTGDGEETGIRRICEAFDQLFAEVPEFTGKVLLENTAGQGTNLGYRFDHL
KSIIEGSSYPTRFGVCFDTCHAFASGYPIADRDGYRRTFDEFDSALGIDKLMAFHLNDSKKGLGCKVDRHEHIGAGALGL
EPFRFILNDPHFKLVPKFIETPKGDADEMDVVNLKLLRSLVEG

Sequences:

>Translated_283_residues
MDLLGAHVSIAGGIHNAVDRGVSSGCGVIQIFTQNSNQWKGKAVSPADAQLFRDKLTASGLSHVVSHDIYLINLAAAPGE
VKDKSLIAFKEEMQRCAALGIDKIVMHPGSHTGDGEETGIRRICEAFDQLFAEVPEFTGKVLLENTAGQGTNLGYRFDHL
KSIIEGSSYPTRFGVCFDTCHAFASGYPIADRDGYRRTFDEFDSALGIDKLMAFHLNDSKKGLGCKVDRHEHIGAGALGL
EPFRFILNDPHFKLVPKFIETPKGDADEMDVVNLKLLRSLVEG
>Mature_283_residues
MDLLGAHVSIAGGIHNAVDRGVSSGCGVIQIFTQNSNQWKGKAVSPADAQLFRDKLTASGLSHVVSHDIYLINLAAAPGE
VKDKSLIAFKEEMQRCAALGIDKIVMHPGSHTGDGEETGIRRICEAFDQLFAEVPEFTGKVLLENTAGQGTNLGYRFDHL
KSIIEGSSYPTRFGVCFDTCHAFASGYPIADRDGYRRTFDEFDSALGIDKLMAFHLNDSKKGLGCKVDRHEHIGAGALGL
EPFRFILNDPHFKLVPKFIETPKGDADEMDVVNLKLLRSLVEG

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=278, Percent_Identity=38.1294964028777, Blast_Score=194, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17531193, Length=269, Percent_Identity=36.0594795539033, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6322735, Length=266, Percent_Identity=35.3383458646617, Blast_Score=163, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_GEOBB (B5EAS8)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137183.1
- ProteinModelPortal:   B5EAS8
- GeneID:   6783182
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0356
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   CLSK924410
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 30819; Mature: 30819

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLGAHVSIAGGIHNAVDRGVSSGCGVIQIFTQNSNQWKGKAVSPADAQLFRDKLTASG
CCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
LSHVVSHDIYLINLAAAPGEVKDKSLIAFKEEMQRCAALGIDKIVMHPGSHTGDGEETGI
HHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCHHHHH
RRICEAFDQLFAEVPEFTGKVLLENTAGQGTNLGYRFDHLKSIIEGSSYPTRFGVCFDTC
HHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
HAFASGYPIADRDGYRRTFDEFDSALGIDKLMAFHLNDSKKGLGCKVDRHEHIGAGALGL
HHHHCCCCCCCCCCHHHHHHHHHHHHCHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCC
EPFRFILNDPHFKLVPKFIETPKGDADEMDVVNLKLLRSLVEG
CHHHHHHCCCCHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLLGAHVSIAGGIHNAVDRGVSSGCGVIQIFTQNSNQWKGKAVSPADAQLFRDKLTASG
CCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
LSHVVSHDIYLINLAAAPGEVKDKSLIAFKEEMQRCAALGIDKIVMHPGSHTGDGEETGI
HHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCHHHHH
RRICEAFDQLFAEVPEFTGKVLLENTAGQGTNLGYRFDHLKSIIEGSSYPTRFGVCFDTC
HHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
HAFASGYPIADRDGYRRTFDEFDSALGIDKLMAFHLNDSKKGLGCKVDRHEHIGAGALGL
HHHHCCCCCCCCCCHHHHHHHHHHHHCHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCC
EPFRFILNDPHFKLVPKFIETPKGDADEMDVVNLKLLRSLVEG
CHHHHHHCCCCHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA