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The map label for this gene is ackA [H]

Identifier: 197116748

GI number: 197116748

Start: 429914

End: 431143

Strand: Direct

Name: ackA [H]

Synonym: Gbem_0348

Alternate gene names: 197116748

Gene position: 429914-431143 (Clockwise)

Preceding gene: 197116747

Following gene: 197116753

Centisome position: 9.32

GC content: 64.88

Gene sequence:

>1230_bases
ATGCTCATCCTCACCCTCAACTGCCGGAGCTTTTCCGTGCAGTATCAACTTTTCGACTGGGGGCAAAACCGCACCCTGGC
CAGCGGGAACGTGGAGCGGATCGTCATCGGGGATACCTTCCTCATCCACAAGGTCCCCGGCAAGGAGCCCAGGCAGCTTG
ACGCCGACTGCGCCGATCACACCCAGGCGCTGCAATTCGTGTTGGACACGCTGACCGACGCGAAGGACGGGGTGCTCCCT
GATGTAGCAAGCATCCGCGCCATCGGGCACCGCGTGGTTCATGGGGGAGAGAGGTTCACCAGGTCGGTGCTGATAGATGA
CGACGTCGTCGCGGCCATCAAGGAGACCGCGAACCTCGCCCCGCTGCACAACACCCCGAACTTGGCCGGCATCCGGGCAG
CGCAGGAGCTCTTGCCCGACCTGCCGCAGGTCGCCATCTTCGACACCGCCTTCCACCAGACCATGCCGGAAAAAGCCTAT
ATCTACCCACTTCCCTACGACTGGTACAAGCAGCACGGCATCAGGCGCTACGGCTTCCACGGGCAGTCACACCTCCATGC
CGCCAGGAGGGGGGCCGCGCTCGCCGGGGTTCCCCTGGAGCGCTGCAACCTGGTGACGGTGCACACCGGCAACGGCGTGT
CGCTCTGCGCGCTGAAAAACGGGGTCTCGGTCGATACCAGCATGGGGCTCACGCCGCTGGAAGGGGTGATGATGGGGACC
CGCTGCGGCGACATAGACGCGGGGATCGTCCCGTTCATGATCAACGAGGCGGGCATCTCGGCGACGGACATGGATCTCTT
CCTGAACCAGAAGAGCGGGCTCGCGGGGATAGTGGGGCGCCGGGTGAGCCGCAGGACGGTGGTGGACGAGGCGGTGGTGG
GAGACGCACGTTGCCAGCTGGCTCTCGACATGGAGTCCTACCGTCTGCGCAAGTACATAGGGGCCTACATCGCCGTGATC
GGCAAGCCCGACGCCATCGTCTTCACCTACGGCGAGGGGTGGGAGGACTGGCCGGTCCGGGGTATGGCGCTCAAGGGGAT
GGAACAGTTCGGCATCGAGGTGGACCTGAAGCGGGACGAGGAGGCCTTGCGGGGCGAGCGGGAGATGCTGATCAGCGCCG
ACAGCTCCAAGGTCAAGGTGTTTGCCCTTCCCAGCGGCGAGGAGATGATGCTCAACGAAGACGTGGCCGCCATTATGGGG
TGGCCGGTGCGCGAGGCGGGTGCTGCCTAG

Upstream 100 bases:

>100_bases
CCCACTTGTAATTGCACTTTTCAACCGAGCATGCTAGTTATGGTCGGTCTAACGAACAAGCGATCGGGCCGCCTGCGCGC
CCGGACTGGAGTCTTTCAGC

Downstream 100 bases:

>100_bases
ACCTTGACCCGGTCCAGGAAGGACTCGACCAGCGGGGTGAAGGTCTGGTGCTCCAAAAGGAAGGCGTCGTGGCCGTAGGC
CGAGGTGATCAGGTGGTACT

Product: propionate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MLILTLNCRSFSVQYQLFDWGQNRTLASGNVERIVIGDTFLIHKVPGKEPRQLDADCADHTQALQFVLDTLTDAKDGVLP
DVASIRAIGHRVVHGGERFTRSVLIDDDVVAAIKETANLAPLHNTPNLAGIRAAQELLPDLPQVAIFDTAFHQTMPEKAY
IYPLPYDWYKQHGIRRYGFHGQSHLHAARRGAALAGVPLERCNLVTVHTGNGVSLCALKNGVSVDTSMGLTPLEGVMMGT
RCGDIDAGIVPFMINEAGISATDMDLFLNQKSGLAGIVGRRVSRRTVVDEAVVGDARCQLALDMESYRLRKYIGAYIAVI
GKPDAIVFTYGEGWEDWPVRGMALKGMEQFGIEVDLKRDEEALRGEREMLISADSSKVKVFALPSGEEMMLNEDVAAIMG
WPVREAGAA

Sequences:

>Translated_409_residues
MLILTLNCRSFSVQYQLFDWGQNRTLASGNVERIVIGDTFLIHKVPGKEPRQLDADCADHTQALQFVLDTLTDAKDGVLP
DVASIRAIGHRVVHGGERFTRSVLIDDDVVAAIKETANLAPLHNTPNLAGIRAAQELLPDLPQVAIFDTAFHQTMPEKAY
IYPLPYDWYKQHGIRRYGFHGQSHLHAARRGAALAGVPLERCNLVTVHTGNGVSLCALKNGVSVDTSMGLTPLEGVMMGT
RCGDIDAGIVPFMINEAGISATDMDLFLNQKSGLAGIVGRRVSRRTVVDEAVVGDARCQLALDMESYRLRKYIGAYIAVI
GKPDAIVFTYGEGWEDWPVRGMALKGMEQFGIEVDLKRDEEALRGEREMLISADSSKVKVFALPSGEEMMLNEDVAAIMG
WPVREAGAA
>Mature_409_residues
MLILTLNCRSFSVQYQLFDWGQNRTLASGNVERIVIGDTFLIHKVPGKEPRQLDADCADHTQALQFVLDTLTDAKDGVLP
DVASIRAIGHRVVHGGERFTRSVLIDDDVVAAIKETANLAPLHNTPNLAGIRAAQELLPDLPQVAIFDTAFHQTMPEKAY
IYPLPYDWYKQHGIRRYGFHGQSHLHAARRGAALAGVPLERCNLVTVHTGNGVSLCALKNGVSVDTSMGLTPLEGVMMGT
RCGDIDAGIVPFMINEAGISATDMDLFLNQKSGLAGIVGRRVSRRTVVDEAVVGDARCQLALDMESYRLRKYIGAYIAVI
GKPDAIVFTYGEGWEDWPVRGMALKGMEQFGIEVDLKRDEEALRGEREMLISADSSKVKVFALPSGEEMMLNEDVAAIMG
WPVREAGAA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=41.25, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI145693184, Length=401, Percent_Identity=37.9052369077307, Blast_Score=275, Evalue=4e-75,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 44745; Mature: 44745

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLILTLNCRSFSVQYQLFDWGQNRTLASGNVERIVIGDTFLIHKVPGKEPRQLDADCADH
CEEEEEECCEEEEEEEEEECCCCCEEECCCCCEEEECCEEEEEECCCCCCCCCCCCHHHH
TQALQFVLDTLTDAKDGVLPDVASIRAIGHRVVHGGERFTRSVLIDDDVVAAIKETANLA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHEEEEEECCHHHHHHHHHCCCC
PLHNTPNLAGIRAAQELLPDLPQVAIFDTAFHQTMPEKAYIYPLPYDWYKQHGIRRYGFH
CCCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHCCCCCEEEEECCCHHHHHCCCEEECCC
GQSHLHAARRGAALAGVPLERCNLVTVHTGNGVSLCALKNGVSVDTSMGLTPLEGVMMGT
CHHHHHHHHCCCEEECCCHHHCCEEEEECCCCEEEEEECCCCEECCCCCCCCHHHEEECC
RCGDIDAGIVPFMINEAGISATDMDLFLNQKSGLAGIVGRRVSRRTVVDEAVVGDARCQL
CCCCCCCCCEEEEEECCCCCEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
ALDMESYRLRKYIGAYIAVIGKPDAIVFTYGEGWEDWPVRGMALKGMEQFGIEVDLKRDE
EECHHHHHHHHHHHEEEEEEECCCEEEEEECCCCCCCCCCCCEECCHHHHCCEEEECCCH
EALRGEREMLISADSSKVKVFALPSGEEMMLNEDVAAIMGWPVREAGAA
HHHCCCEEEEEECCCCEEEEEECCCCCCEEECCCHHHHHCCCCHHCCCC
>Mature Secondary Structure
MLILTLNCRSFSVQYQLFDWGQNRTLASGNVERIVIGDTFLIHKVPGKEPRQLDADCADH
CEEEEEECCEEEEEEEEEECCCCCEEECCCCCEEEECCEEEEEECCCCCCCCCCCCHHHH
TQALQFVLDTLTDAKDGVLPDVASIRAIGHRVVHGGERFTRSVLIDDDVVAAIKETANLA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHEEEEEECCHHHHHHHHHCCCC
PLHNTPNLAGIRAAQELLPDLPQVAIFDTAFHQTMPEKAYIYPLPYDWYKQHGIRRYGFH
CCCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHCCCCCEEEEECCCHHHHHCCCEEECCC
GQSHLHAARRGAALAGVPLERCNLVTVHTGNGVSLCALKNGVSVDTSMGLTPLEGVMMGT
CHHHHHHHHCCCEEECCCHHHCCEEEEECCCCEEEEEECCCCEECCCCCCCCHHHEEECC
RCGDIDAGIVPFMINEAGISATDMDLFLNQKSGLAGIVGRRVSRRTVVDEAVVGDARCQL
CCCCCCCCCEEEEEECCCCCEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
ALDMESYRLRKYIGAYIAVIGKPDAIVFTYGEGWEDWPVRGMALKGMEQFGIEVDLKRDE
EECHHHHHHHHHHHEEEEEEECCCEEEEEECCCCCCCCCCCCEECCHHHHCCEEEECCCH
EALRGEREMLISADSSKVKVFALPSGEEMMLNEDVAAIMGWPVREAGAA
HHHCCCEEEEEECCCCEEEEEECCCCCCEEECCCHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA