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The map label for this gene is nasA [H]

Identifier: 197104434

GI number: 197104434

Start: 1064949

End: 1066331

Strand: Direct

Name: nasA [H]

Synonym: PHZ_c0968

Alternate gene names: 197104434

Gene position: 1064949-1066331 (Clockwise)

Preceding gene: 197104433

Following gene: 197104435

Centisome position: 26.65

GC content: 73.1

Gene sequence:

>1383_bases
GTGGGCGCCGGGGCCGGTATCGGGGTGACGAGCCGCCGAGGCGGCGTTTTCATCGAACCCAGGCCCAGGACAACGGCGTT
CGCGGCCCCCGGACAGGCTCAAGGACGAGCCCTCCCACACGGCGACGCCGCCGCTCCACCGCAATCCGCACGGGGCCTGA
TCCGCATGTCTGCGCGCTCGTTCCTGAAGGCCGGCCACCCGCCGACCCTCTTCGCCGCCTTCCTCTATTTCGACCTGAGC
TTCATGGTCTGGGTGATCCTGGGGCCGCTCGGCGTCGCCATCGCCACGGACTTCGGCCTGACGCCGGCCGAGAAGGGGCT
GATGGTCGCCGTGCCGGTGCTGGCCGGCGCCGTGCTGCGCCTCTTCGCGGGCGTGCTTGTGGATCACATCGGTCCCAAGC
GGACCGGCCTGATCGGCCAGCTCGTCGTGCTGGCGGGCCTCGCGGCCGCCTGGCGGCTGGGGATCGGCGGCTATCCCCAG
GTCCTGGCCCTCGGCTGCGTGCTGGGCGTCGCGGGGGCGTCCTTCGCTGTCGCCCTGCCGCTCGCTTCGCGCTGGTACCC
GCCCGAACACCAGGGCCTGGCCCTCGGTATCGCCGGCGCCGGCAATTCGGGGACGGCCCTCGCGGCCCTGTTCGCCCCAG
CCCTGGCCCAGCGCTTCGGCTGGGAGAATGTGATCGGCCTGGCGACCCTGCCGCTCGCCGCAGCCTTCGTCGTCTATCTG
GTCTTCGCCAGGGACGCGCCGGACCGGCCGCCGCCCAAGCGCCTCTCGGAATACCTCTCGGTGCTCCGCACGGCCGACGC
CTGGTGGCTGATGCTGCTCTACGGCGTCACCTTCGGCGGGTTCGTGGGCCTGTCGTCCTCCCTGACCATCTACTTCAACG
CCGAGTACGGCCTGCCGGCTGTGACCGCGGGCTTCTTCACCGCCGCCTGCGTGTTCGCCGGCTCGTTCGTTCGGCCGGTG
GGCGGGGCGCTGGCCGACCGCTTCGGCGGGGTGCGCACGCTGAGCGTCGTCTACCTGCTGGCCGCGCTCGGCCTCGCGCT
GGCCAGTTTCCAGCCGCCCAGCGTCTGGATGGCCCTGGCCGTGATCCTCTTCGCCATGGCGGCGCTCGGGGCCGGCAACG
GGGCGGTCTTCCAGCTCGCGCCGCAGCGCTTCGGCAAGGAGATCGGCGTGGTCACCGGCCTCGTCGGCATGACCGGCGGG
ATCGGCGGCTTCTACCTGGCCTCCAGCCTCGGCTGGGCCAAGCAGGTCACCGGCAGCTACCAGGCGGGCCTGCTCGCCTT
CGCCGCCCTCGCCCTCGTGGCCCTGGCCTGCCTCACCGGGGTCAAGGCCCGCTGGCGGCGCACCTGGGCCGGCGAGGCCG
CCGGCGCGCCCCTCAGGCTCTAG

Upstream 100 bases:

>100_bases
CGCCCGGACCTCCACGCCGCGGCGGCCGGGGCGCTCAGCGCCTGAATTCTGGTCATCGCGCCGCCTACGGCGCCGTGATT
TTGCACTGCGGCATGGCCCC

Downstream 100 bases:

>100_bases
GAGGCGATCATGACCACCCTCAGGGAACGCCTCGTCGTCGTCGGCAACGGCATGGCCGGCTGCCGGGCGGTGCAGGAGGT
GCTGAAGCGCGACCCCGGCC

Product: nitrate transporter, NarK/NasA family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MGAGAGIGVTSRRGGVFIEPRPRTTAFAAPGQAQGRALPHGDAAAPPQSARGLIRMSARSFLKAGHPPTLFAAFLYFDLS
FMVWVILGPLGVAIATDFGLTPAEKGLMVAVPVLAGAVLRLFAGVLVDHIGPKRTGLIGQLVVLAGLAAAWRLGIGGYPQ
VLALGCVLGVAGASFAVALPLASRWYPPEHQGLALGIAGAGNSGTALAALFAPALAQRFGWENVIGLATLPLAAAFVVYL
VFARDAPDRPPPKRLSEYLSVLRTADAWWLMLLYGVTFGGFVGLSSSLTIYFNAEYGLPAVTAGFFTAACVFAGSFVRPV
GGALADRFGGVRTLSVVYLLAALGLALASFQPPSVWMALAVILFAMAALGAGNGAVFQLAPQRFGKEIGVVTGLVGMTGG
IGGFYLASSLGWAKQVTGSYQAGLLAFAALALVALACLTGVKARWRRTWAGEAAGAPLRL

Sequences:

>Translated_460_residues
MGAGAGIGVTSRRGGVFIEPRPRTTAFAAPGQAQGRALPHGDAAAPPQSARGLIRMSARSFLKAGHPPTLFAAFLYFDLS
FMVWVILGPLGVAIATDFGLTPAEKGLMVAVPVLAGAVLRLFAGVLVDHIGPKRTGLIGQLVVLAGLAAAWRLGIGGYPQ
VLALGCVLGVAGASFAVALPLASRWYPPEHQGLALGIAGAGNSGTALAALFAPALAQRFGWENVIGLATLPLAAAFVVYL
VFARDAPDRPPPKRLSEYLSVLRTADAWWLMLLYGVTFGGFVGLSSSLTIYFNAEYGLPAVTAGFFTAACVFAGSFVRPV
GGALADRFGGVRTLSVVYLLAALGLALASFQPPSVWMALAVILFAMAALGAGNGAVFQLAPQRFGKEIGVVTGLVGMTGG
IGGFYLASSLGWAKQVTGSYQAGLLAFAALALVALACLTGVKARWRRTWAGEAAGAPLRL
>Mature_459_residues
GAGAGIGVTSRRGGVFIEPRPRTTAFAAPGQAQGRALPHGDAAAPPQSARGLIRMSARSFLKAGHPPTLFAAFLYFDLSF
MVWVILGPLGVAIATDFGLTPAEKGLMVAVPVLAGAVLRLFAGVLVDHIGPKRTGLIGQLVVLAGLAAAWRLGIGGYPQV
LALGCVLGVAGASFAVALPLASRWYPPEHQGLALGIAGAGNSGTALAALFAPALAQRFGWENVIGLATLPLAAAFVVYLV
FARDAPDRPPPKRLSEYLSVLRTADAWWLMLLYGVTFGGFVGLSSSLTIYFNAEYGLPAVTAGFFTAACVFAGSFVRPVG
GALADRFGGVRTLSVVYLLAALGLALASFQPPSVWMALAVILFAMAALGAGNGAVFQLAPQRFGKEIGVVTGLVGMTGGI
GGFYLASSLGWAKQVTGSYQAGLLAFAALALVALACLTGVKARWRRTWAGEAAGAPLRL

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=394, Percent_Identity=26.3959390862944, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47222; Mature: 47091

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAGAGIGVTSRRGGVFIEPRPRTTAFAAPGQAQGRALPHGDAAAPPQSARGLIRMSARS
CCCCCCCCCCCCCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
FLKAGHPPTLFAAFLYFDLSFMVWVILGPLGVAIATDFGLTPAEKGLMVAVPVLAGAVLR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHH
LFAGVLVDHIGPKRTGLIGQLVVLAGLAAAWRLGIGGYPQVLALGCVLGVAGASFAVALP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHH
LASRWYPPEHQGLALGIAGAGNSGTALAALFAPALAQRFGWENVIGLATLPLAAAFVVYL
HHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VFARDAPDRPPPKRLSEYLSVLRTADAWWLMLLYGVTFGGFVGLSSSLTIYFNAEYGLPA
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
VTAGFFTAACVFAGSFVRPVGGALADRFGGVRTLSVVYLLAALGLALASFQPPSVWMALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VILFAMAALGAGNGAVFQLAPQRFGKEIGVVTGLVGMTGGIGGFYLASSLGWAKQVTGSY
HHHHHHHHHCCCCCCEEEECHHHHCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCH
QAGLLAFAALALVALACLTGVKARWRRTWAGEAAGAPLRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GAGAGIGVTSRRGGVFIEPRPRTTAFAAPGQAQGRALPHGDAAAPPQSARGLIRMSARS
CCCCCCCCCCCCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
FLKAGHPPTLFAAFLYFDLSFMVWVILGPLGVAIATDFGLTPAEKGLMVAVPVLAGAVLR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHH
LFAGVLVDHIGPKRTGLIGQLVVLAGLAAAWRLGIGGYPQVLALGCVLGVAGASFAVALP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHH
LASRWYPPEHQGLALGIAGAGNSGTALAALFAPALAQRFGWENVIGLATLPLAAAFVVYL
HHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VFARDAPDRPPPKRLSEYLSVLRTADAWWLMLLYGVTFGGFVGLSSSLTIYFNAEYGLPA
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
VTAGFFTAACVFAGSFVRPVGGALADRFGGVRTLSVVYLLAALGLALASFQPPSVWMALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VILFAMAALGAGNGAVFQLAPQRFGKEIGVVTGLVGMTGGIGGFYLASSLGWAKQVTGSY
HHHHHHHHHCCCCCCEEEECHHHHCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCH
QAGLLAFAALALVALACLTGVKARWRRTWAGEAAGAPLRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]