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The map label for this gene is 197104220

Identifier: 197104220

GI number: 197104220

Start: 829276

End: 830667

Strand: Direct

Name: 197104220

Synonym: PHZ_c0754

Alternate gene names: NA

Gene position: 829276-830667 (Clockwise)

Preceding gene: 197104215

Following gene: 197104222

Centisome position: 20.75

GC content: 70.47

Gene sequence:

>1392_bases
ATGGACGGCGACGCTCGCATCAATCCCTACCTGACGGGCAACTTCGCCCCGGTCCGCTCGGAGGACGACTTCGACCTGCC
TGTCGTGGGCGAGATCCCGCGCGAGCTGCGCGGCGCGCTGTTCCGCATCGGCCCCAATCCGCAGTTCGAGCCGCGCGACC
CGAACCACCACTGGTTCTCGGGCGACGGCATGGTCCACGGCTTCTATGTCGAGGACGGCAAGGTCCGGTATCGCAACCGC
TACGTCCGCACGCCCAAGTGGCAGGCCGAGAACGCCGCCGGCCGCTCGCTGTTCGGCAGCTTCGGCAATCCCATGACCAC
CGATCCCTCGGCCCTAGGCCAGGACTCGGGCGTCGCCAACACCAACATCGTCTGGCACGCCGGCCGGCTGATGGCGCTCG
AGGAAGGCCACATGCCGACGCAGATGGACCCGGGCACGCTGGAGACCATCGGCTACGCCGAAGCCTACCGCGGCCGCACG
ACGGCCCATCCCAAGCTCGACCCGGTGTCGGGCGAGATGGTCTGGTTCGCCTATGGCGTCGGCGACATGCCCCTGTCGGC
CGGCATGAGCTACGGCGTGACCGGCGCCGACGGCAAGGTCGTGCGCCGCGACGACTTCCAGGCGCCCTTCGCCTGCATGG
TCCACGACTTCATGGTCACGCAGAACCACGTCCTCTTCCCCATCCTGCCGCTGACCGCCAGCCTCGAGCGGGCGATGAGC
GGCAAGCCCGCCTTCGCCTGGGAGCCCGAGAAGGGCGCCTACGTGGGGGTGATGCGCCGCGACGGCGACGTCGGCTCGAT
CCGCTGGTTCAACACCGAGGCCTGCTACGTCTTCCACCCGCTGAACGCCTGGGAGGAGGAGGGCCGGATCGTCGCCGACG
TGATGCGCTACGACGCCGCGCCGCTGTTCCCCAACGCCGATGGCTCTCCGGGCCGCAAGACGGCGGCGCGGCTGGTCCGC
TGGACGTTCGACCTGGCCGGTAACAGCGACGCCATCAAAGAGGAGCCGCTGGACGACCTCGACGGCGAGTTCCCGAGGGT
CGATCCGCGCGTCGAGACGCTGCGCCACCGCCACGGCTGGTACGCGGCCGACCCGACGAACGGCAAGACCATCAAGCAGG
GCGCGATCGCGCACCTCGACCTGACCACCGGCGCGCGCCAGGTCTATGCGCTGCCGGACGGGGACCTGACCTCAGAGCCC
GTGTTCACGCCCCGGTCGGCCGACGCGCCCGAGGGCGACGGTTGGGTCACGGCCGTGGTCTGGCGCGCGGCCGAGAACCG
CTCGGACCTGCTGGTCTTCGAGGCGCAGGACATCGCCAGGGGGCCGGTCGCGACGGCGGCCATGCCGCGGCGCGTGCCCT
TCGGCTTCCACGGCAACTGGGTGTCGCTCTAG

Upstream 100 bases:

>100_bases
AGGCATGGAGACTCAGGGTTCGAAAAAAGCACGCTTGCCAAGGCGTAGACCTTACCTCATAAATTTACAACGTAAGTTGT
TCGGCTGAGGAGGAACGGCC

Downstream 100 bases:

>100_bases
CGCCTAGCCGCGGAACTCCAGCGCCTGGAAGCTGGCGGGCGTGGTGGGCGCGAGGAGGAAGCGGACCTCCAGCGTTCCCC
GCTCGCACGGGAACTTCAGG

Product: retinal pigment epithelial membrane protein

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MDGDARINPYLTGNFAPVRSEDDFDLPVVGEIPRELRGALFRIGPNPQFEPRDPNHHWFSGDGMVHGFYVEDGKVRYRNR
YVRTPKWQAENAAGRSLFGSFGNPMTTDPSALGQDSGVANTNIVWHAGRLMALEEGHMPTQMDPGTLETIGYAEAYRGRT
TAHPKLDPVSGEMVWFAYGVGDMPLSAGMSYGVTGADGKVVRRDDFQAPFACMVHDFMVTQNHVLFPILPLTASLERAMS
GKPAFAWEPEKGAYVGVMRRDGDVGSIRWFNTEACYVFHPLNAWEEEGRIVADVMRYDAAPLFPNADGSPGRKTAARLVR
WTFDLAGNSDAIKEEPLDDLDGEFPRVDPRVETLRHRHGWYAADPTNGKTIKQGAIAHLDLTTGARQVYALPDGDLTSEP
VFTPRSADAPEGDGWVTAVVWRAAENRSDLLVFEAQDIARGPVATAAMPRRVPFGFHGNWVSL

Sequences:

>Translated_463_residues
MDGDARINPYLTGNFAPVRSEDDFDLPVVGEIPRELRGALFRIGPNPQFEPRDPNHHWFSGDGMVHGFYVEDGKVRYRNR
YVRTPKWQAENAAGRSLFGSFGNPMTTDPSALGQDSGVANTNIVWHAGRLMALEEGHMPTQMDPGTLETIGYAEAYRGRT
TAHPKLDPVSGEMVWFAYGVGDMPLSAGMSYGVTGADGKVVRRDDFQAPFACMVHDFMVTQNHVLFPILPLTASLERAMS
GKPAFAWEPEKGAYVGVMRRDGDVGSIRWFNTEACYVFHPLNAWEEEGRIVADVMRYDAAPLFPNADGSPGRKTAARLVR
WTFDLAGNSDAIKEEPLDDLDGEFPRVDPRVETLRHRHGWYAADPTNGKTIKQGAIAHLDLTTGARQVYALPDGDLTSEP
VFTPRSADAPEGDGWVTAVVWRAAENRSDLLVFEAQDIARGPVATAAMPRRVPFGFHGNWVSL
>Mature_463_residues
MDGDARINPYLTGNFAPVRSEDDFDLPVVGEIPRELRGALFRIGPNPQFEPRDPNHHWFSGDGMVHGFYVEDGKVRYRNR
YVRTPKWQAENAAGRSLFGSFGNPMTTDPSALGQDSGVANTNIVWHAGRLMALEEGHMPTQMDPGTLETIGYAEAYRGRT
TAHPKLDPVSGEMVWFAYGVGDMPLSAGMSYGVTGADGKVVRRDDFQAPFACMVHDFMVTQNHVLFPILPLTASLERAMS
GKPAFAWEPEKGAYVGVMRRDGDVGSIRWFNTEACYVFHPLNAWEEEGRIVADVMRYDAAPLFPNADGSPGRKTAARLVR
WTFDLAGNSDAIKEEPLDDLDGEFPRVDPRVETLRHRHGWYAADPTNGKTIKQGAIAHLDLTTGARQVYALPDGDLTSEP
VFTPRSADAPEGDGWVTAVVWRAAENRSDLLVFEAQDIARGPVATAAMPRRVPFGFHGNWVSL

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI82617628, Length=521, Percent_Identity=24.1842610364683, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI82617624, Length=521, Percent_Identity=24.1842610364683, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI4506591, Length=514, Percent_Identity=22.7626459143969, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32564756, Length=507, Percent_Identity=20.9072978303748, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 51121; Mature: 51121

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGDARINPYLTGNFAPVRSEDDFDLPVVGEIPRELRGALFRIGPNPQFEPRDPNHHWFS
CCCCCEECCEEECCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECCCCCCCCCCCCCCEEC
GDGMVHGFYVEDGKVRYRNRYVRTPKWQAENAAGRSLFGSFGNPMTTDPSALGQDSGVAN
CCCEEEEEEEECCEEEECCEEECCCCCCCCCCCCHHHHHCCCCCCCCCHHHCCCCCCCCC
TNIVWHAGRLMALEEGHMPTQMDPGTLETIGYAEAYRGRTTAHPKLDPVSGEMVWFAYGV
CEEEEECCEEEEEECCCCCCCCCCCCCHHCCHHHHHCCCCCCCCCCCCCCCCEEEEEECC
GDMPLSAGMSYGVTGADGKVVRRDDFQAPFACMVHDFMVTQNHVLFPILPLTASLERAMS
CCCCHHCCCCCCEECCCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHC
GKPAFAWEPEKGAYVGVMRRDGDVGSIRWFNTEACYVFHPLNAWEEEGRIVADVMRYDAA
CCCCEEECCCCCCEEEEEEECCCCCEEEEECCCEEEEEECCCCHHHCCCEEEHHHHHCCC
PLFPNADGSPGRKTAARLVRWTFDLAGNSDAIKEEPLDDLDGEFPRVDPRVETLRHRHGW
CCCCCCCCCCCHHHHHHHEEEEEEECCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCE
YAADPTNGKTIKQGAIAHLDLTTGARQVYALPDGDLTSEPVFTPRSADAPEGDGWVTAVV
EEECCCCCCEECCCCEEEEEECCCCEEEEECCCCCCCCCCEECCCCCCCCCCCCEEEEEE
WRAAENRSDLLVFEAQDIARGPVATAAMPRRVPFGFHGNWVSL
EEECCCCCCEEEEECCHHCCCCCHHHCCCCCCCCCCCCCEECC
>Mature Secondary Structure
MDGDARINPYLTGNFAPVRSEDDFDLPVVGEIPRELRGALFRIGPNPQFEPRDPNHHWFS
CCCCCEECCEEECCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECCCCCCCCCCCCCCEEC
GDGMVHGFYVEDGKVRYRNRYVRTPKWQAENAAGRSLFGSFGNPMTTDPSALGQDSGVAN
CCCEEEEEEEECCEEEECCEEECCCCCCCCCCCCHHHHHCCCCCCCCCHHHCCCCCCCCC
TNIVWHAGRLMALEEGHMPTQMDPGTLETIGYAEAYRGRTTAHPKLDPVSGEMVWFAYGV
CEEEEECCEEEEEECCCCCCCCCCCCCHHCCHHHHHCCCCCCCCCCCCCCCCEEEEEECC
GDMPLSAGMSYGVTGADGKVVRRDDFQAPFACMVHDFMVTQNHVLFPILPLTASLERAMS
CCCCHHCCCCCCEECCCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHC
GKPAFAWEPEKGAYVGVMRRDGDVGSIRWFNTEACYVFHPLNAWEEEGRIVADVMRYDAA
CCCCEEECCCCCCEEEEEEECCCCCEEEEECCCEEEEEECCCCHHHCCCEEEHHHHHCCC
PLFPNADGSPGRKTAARLVRWTFDLAGNSDAIKEEPLDDLDGEFPRVDPRVETLRHRHGW
CCCCCCCCCCCHHHHHHHEEEEEEECCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCE
YAADPTNGKTIKQGAIAHLDLTTGARQVYALPDGDLTSEPVFTPRSADAPEGDGWVTAVV
EEECCCCCCEECCCCEEEEEECCCCEEEEECCCCCCCCCCEECCCCCCCCCCCCEEEEEE
WRAAENRSDLLVFEAQDIARGPVATAAMPRRVPFGFHGNWVSL
EEECCCCCCEEEEECCHHCCCCCHHHCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]