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The map label for this gene is psrB [H]

Identifier: 195952686

GI number: 195952686

Start: 268377

End: 269048

Strand: Direct

Name: psrB [H]

Synonym: HY04AAS1_0308

Alternate gene names: 195952686

Gene position: 268377-269048 (Clockwise)

Preceding gene: 195952685

Following gene: 195952687

Centisome position: 17.21

GC content: 41.07

Gene sequence:

>672_bases
ATGGAAAAGGTTATACCAAAATGGGTGTTCGTGGTGGATTTAAACTCTTGTGTAGGATGCAATGCTTGTATGGCAGCTTG
CGCCCAAGAAAATCAAACGCCTTATTGGTCAGAAAAGTGGAGAACAAAAGTAGAGCAAATAGAAATAGGCGAGTTTCCGG
AGACAAAACGTGTATTTTTCCCACATCTTTGTATGCAGTGCCAAAACACTCCTTGCTATTACGCTTGTCCTACTGGTGCC
ACTTACAAAACCGAAGAGGGTATTGTGCTTGTAAACCACGAAAGATGTATAGGCTGTGAGGCTTGTGTAATAGCTTGTCC
TTATGGAGCAAGATATCCTTATGAATCTGATGATGTAGACGAATGTGAAAAGTTGTACGGTGAAGAGGCAAGGCATACCA
CACCTCACATAGACAAATGCACATGGTGTTATCATAGGCTAAAAGAAGGTTTAGAACCAGCTTGTGTAGAAACATGTCCT
ACCAACTGCCGTATGTTTGGAGATTTAAACGTAGATTCTTACGTATCTAGGCTTGTATTAGAAGGTAAGGCTATACCGTT
AAGCCCTCAGCTTGGTACAGAGCCAAAAGTGTTTTATGTAACCTCAAAAGAAACACCAATAGGCGTTGCCTCTCATCACC
TTGATGAACCACCATACAAACAAACTATTTAA

Upstream 100 bases:

>100_bases
TGATCTGATACCCTATAGACCAGAACCGGTTATAGGTGGATATAGAGCAAACGAATTTACTGTAAAAGTTGTGAAGGCTT
AAATGTTTTAGGAGGCTTTT

Downstream 100 bases:

>100_bases
AGGAGGTAAAACATGGACGGACTTACTATGTATTTTGGTAACTATCAGCACTTTGTGCATCAAACAGCTTGGGGATGGAT
GATAGCTGTTTATCTTTTCT

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Sulfur reductase chain B [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MEKVIPKWVFVVDLNSCVGCNACMAACAQENQTPYWSEKWRTKVEQIEIGEFPETKRVFFPHLCMQCQNTPCYYACPTGA
TYKTEEGIVLVNHERCIGCEACVIACPYGARYPYESDDVDECEKLYGEEARHTTPHIDKCTWCYHRLKEGLEPACVETCP
TNCRMFGDLNVDSYVSRLVLEGKAIPLSPQLGTEPKVFYVTSKETPIGVASHHLDEPPYKQTI

Sequences:

>Translated_223_residues
MEKVIPKWVFVVDLNSCVGCNACMAACAQENQTPYWSEKWRTKVEQIEIGEFPETKRVFFPHLCMQCQNTPCYYACPTGA
TYKTEEGIVLVNHERCIGCEACVIACPYGARYPYESDDVDECEKLYGEEARHTTPHIDKCTWCYHRLKEGLEPACVETCP
TNCRMFGDLNVDSYVSRLVLEGKAIPLSPQLGTEPKVFYVTSKETPIGVASHHLDEPPYKQTI
>Mature_223_residues
MEKVIPKWVFVVDLNSCVGCNACMAACAQENQTPYWSEKWRTKVEQIEIGEFPETKRVFFPHLCMQCQNTPCYYACPTGA
TYKTEEGIVLVNHERCIGCEACVIACPYGARYPYESDDVDECEKLYGEEARHTTPHIDKCTWCYHRLKEGLEPACVETCP
TNCRMFGDLNVDSYVSRLVLEGKAIPLSPQLGTEPKVFYVTSKETPIGVASHHLDEPPYKQTI

Specific function: Component of the phosphorylative electron transport system with polysulfide as the terminal acceptor [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=200, Percent_Identity=35.5, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI226510944, Length=163, Percent_Identity=34.3558282208589, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1787122, Length=221, Percent_Identity=28.9592760180996, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787872, Length=220, Percent_Identity=28.6363636363636, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082179, Length=160, Percent_Identity=35, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1789067, Length=166, Percent_Identity=34.9397590361446, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1787749, Length=229, Percent_Identity=27.9475982532751, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790326, Length=173, Percent_Identity=30.0578034682081, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787740, Length=125, Percent_Identity=34.4, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787478, Length=189, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI2367245, Length=162, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082180, Length=158, Percent_Identity=32.2784810126582, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082114, Length=166, Percent_Identity=30.7228915662651, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788811, Length=158, Percent_Identity=27.8481012658228, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25295; Mature: 25295

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
9.9 %Cys+Met (Translated Protein)
8.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
9.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVIPKWVFVVDLNSCVGCNACMAACAQENQTPYWSEKWRTKVEQIEIGEFPETKRVFF
CCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHEEECCCCCCCHHHHH
PHLCMQCQNTPCYYACPTGATYKTEEGIVLVNHERCIGCEACVIACPYGARYPYESDDVD
HHHHHHHCCCCEEEECCCCCCEECCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCHH
ECEKLYGEEARHTTPHIDKCTWCYHRLKEGLEPACVETCPTNCRMFGDLNVDSYVSRLVL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCHHHHHHHHHH
EGKAIPLSPQLGTEPKVFYVTSKETPIGVASHHLDEPPYKQTI
CCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEKVIPKWVFVVDLNSCVGCNACMAACAQENQTPYWSEKWRTKVEQIEIGEFPETKRVFF
CCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHEEECCCCCCCHHHHH
PHLCMQCQNTPCYYACPTGATYKTEEGIVLVNHERCIGCEACVIACPYGARYPYESDDVD
HHHHHHHCCCCEEEECCCCCCEECCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCHH
ECEKLYGEEARHTTPHIDKCTWCYHRLKEGLEPACVETCPTNCRMFGDLNVDSYVSRLVL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCHHHHHHHHHH
EGKAIPLSPQLGTEPKVFYVTSKETPIGVASHHLDEPPYKQTI
CCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1597189; 14500908 [H]