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The map label for this gene is nrfD [H]

Identifier: 195952687

GI number: 195952687

Start: 269061

End: 269960

Strand: Direct

Name: nrfD [H]

Synonym: HY04AAS1_0309

Alternate gene names: 195952687

Gene position: 269061-269960 (Clockwise)

Preceding gene: 195952686

Following gene: 195952688

Centisome position: 17.25

GC content: 36.33

Gene sequence:

>900_bases
ATGGACGGACTTACTATGTATTTTGGTAACTATCAGCACTTTGTGCATCAAACAGCTTGGGGATGGATGATAGCTGTTTA
TCTTTTCTTAGGTGGCCTTGGTGGTGCCATGGGTGCTTTGTCTTCTTATTTTTACCTTATAAAAAAAGAAAATAACCCGG
TGCTATTTGGGCTTTCTACACTTCTTGGTATAGGTCTTGTAAACTTTGGTGGTATATTTTTGCTTATACATATGCTCAAA
CCTTGGTTTGCGTTTTTTGTATGGACTCATCCTACCAGCTGGATATTTTGGGGTGCATCCTTTATTATGATATACACTAT
CACAGGTGCCATTTGGGGACTTTCGCTTATATCAGCTCATATTAACTTTCCTTTTTCTCAAAAGCTTAAAAACCTAAACA
CTTCTTTAGTGGATAAACTTGGGTATATAAGCGGTGTTATGGGACTTTTAACGGCTGTTTATACTGGGCTTTTGCTTTCT
ACTGCACCAGCCATAACGCTTTGGTCAAATCCTGGCTTACCGGTACTTTTTATGGTATCTGCTCTTTCTACTGCTACAGC
TTATTTTATGCTGGTATCTCCTTCTCATATAGCTCATGAAAATGAAAAACTTGATATAGCTTTACTTGCATTAGAGATTA
TCATAATATCCGCTTTCTTTAACTACCTATTTATATCAAGTGCCGCTGGTAAATTTATAATCTCCAAAATGCTTTCTTCT
ATTGGTTTCATGGGGTTTTTTGTAACACTTGGTCTTTTGGTGCCGTTTTTGCTTGAAATTTATGCCATCAAAAAGCACTC
AAAATCTTTAGTAATATTGGCTTCTTTACTCGTTTTGATGGGCGGGTTTTTGCTTAGATTTTACATTCTAAAATTTGGAT
ATTACACATACCCATGGTAA

Upstream 100 bases:

>100_bases
AAAAGTGTTTTATGTAACCTCAAAAGAAACACCAATAGGCGTTGCCTCTCATCACCTTGATGAACCACCATACAAACAAA
CTATTTAAAGGAGGTAAAAC

Downstream 100 bases:

>100_bases
ATGTTTTTGGGCTTTTAGAACTAAAATTTCCTATTGTTTTTGACGAAAAAAAGTGTATCAATGTGATAAAATCAAAAAAA
GAGTGTGATGCTTGTGAGAA

Product: Polysulfide reductase NrfD

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDGLTMYFGNYQHFVHQTAWGWMIAVYLFLGGLGGAMGALSSYFYLIKKENNPVLFGLSTLLGIGLVNFGGIFLLIHMLK
PWFAFFVWTHPTSWIFWGASFIMIYTITGAIWGLSLISAHINFPFSQKLKNLNTSLVDKLGYISGVMGLLTAVYTGLLLS
TAPAITLWSNPGLPVLFMVSALSTATAYFMLVSPSHIAHENEKLDIALLALEIIIISAFFNYLFISSAAGKFIISKMLSS
IGFMGFFVTLGLLVPFLLEIYAIKKHSKSLVILASLLVLMGGFLLRFYILKFGYYTYPW

Sequences:

>Translated_299_residues
MDGLTMYFGNYQHFVHQTAWGWMIAVYLFLGGLGGAMGALSSYFYLIKKENNPVLFGLSTLLGIGLVNFGGIFLLIHMLK
PWFAFFVWTHPTSWIFWGASFIMIYTITGAIWGLSLISAHINFPFSQKLKNLNTSLVDKLGYISGVMGLLTAVYTGLLLS
TAPAITLWSNPGLPVLFMVSALSTATAYFMLVSPSHIAHENEKLDIALLALEIIIISAFFNYLFISSAAGKFIISKMLSS
IGFMGFFVTLGLLVPFLLEIYAIKKHSKSLVILASLLVLMGGFLLRFYILKFGYYTYPW
>Mature_299_residues
MDGLTMYFGNYQHFVHQTAWGWMIAVYLFLGGLGGAMGALSSYFYLIKKENNPVLFGLSTLLGIGLVNFGGIFLLIHMLK
PWFAFFVWTHPTSWIFWGASFIMIYTITGAIWGLSLISAHINFPFSQKLKNLNTSLVDKLGYISGVMGLLTAVYTGLLLS
TAPAITLWSNPGLPVLFMVSALSTATAYFMLVSPSHIAHENEKLDIALLALEIIIISAFFNYLFISSAAGKFIISKMLSS
IGFMGFFVTLGLLVPFLLEIYAIKKHSKSLVILASLLVLMGGFLLRFYILKFGYYTYPW

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=323, Percent_Identity=27.8637770897833, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 33253; Mature: 33253

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGLTMYFGNYQHFVHQTAWGWMIAVYLFLGGLGGAMGALSSYFYLIKKENNPVLFGLST
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEEECCCCEEEEHHH
LLGIGLVNFGGIFLLIHMLKPWFAFFVWTHPTSWIFWGASFIMIYTITGAIWGLSLISAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INFPFSQKLKNLNTSLVDKLGYISGVMGLLTAVYTGLLLSTAPAITLWSNPGLPVLFMVS
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHH
ALSTATAYFMLVSPSHIAHENEKLDIALLALEIIIISAFFNYLFISSAAGKFIISKMLSS
HHHHHHHHHEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IGFMGFFVTLGLLVPFLLEIYAIKKHSKSLVILASLLVLMGGFLLRFYILKFGYYTYPW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDGLTMYFGNYQHFVHQTAWGWMIAVYLFLGGLGGAMGALSSYFYLIKKENNPVLFGLST
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEEECCCCEEEEHHH
LLGIGLVNFGGIFLLIHMLKPWFAFFVWTHPTSWIFWGASFIMIYTITGAIWGLSLISAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INFPFSQKLKNLNTSLVDKLGYISGVMGLLTAVYTGLLLSTAPAITLWSNPGLPVLFMVS
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHH
ALSTATAYFMLVSPSHIAHENEKLDIALLALEIIIISAFFNYLFISSAAGKFIISKMLSS
HHHHHHHHHEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IGFMGFFVTLGLLVPFLLEIYAIKKHSKSLVILASLLVLMGGFLLRFYILKFGYYTYPW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]