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The map label for this gene is hyaB [H]

Identifier: 194737319

GI number: 194737319

Start: 1863950

End: 1865743

Strand: Direct

Name: hyaB [H]

Synonym: SeSA_A1929

Alternate gene names: 194737319

Gene position: 1863950-1865743 (Clockwise)

Preceding gene: 194737959

Following gene: 194736924

Centisome position: 39.58

GC content: 55.41

Gene sequence:

>1794_bases
ATGAGTAACCAGTATCAAACCCAGGGTTATACCGTTAACGACGCCGGCCGTCGTCTGATCGTGGACCCGATTACCCGCAT
CGAAGGGCATATGCGCTGCGAAGTGAACATTGATGAGCAAAACGTCATTACCAATGCCGTTTCCTGCGGCACGATGTTCC
GCGGGCTGGAGATCATTCTGCAAGGCCGTGACCCGCGTGACGCCTGGGCGTTTGTTGAACGTATTTGTGGTGTCTGTACC
GGGGTACATGCTCTGGCATCGGTGTACGCTATCGAAGATGCGATTGGTATCCAGGTGCCGGATAACGCCAATATTATACG
TAACATCATGCTGGCTACGTTGTGGTGTCACGATCATCTGGTCCATTTTTATCAGTTAGCCGGTATGGACTGGATTGATG
TTCTGAATGCGCTGAAAGCCGATCCGCGCGCCACCTCGCAGTTGGCGCAAAGCCTTTCCGCCTGGCCGATGTCATCGCCG
GGCTACTTCTTTGACGTACAAAATCGATTGAAGAAATTTGTCGATGGCGGCCAGCTTGGGATATTCCGTAACGGCTATTG
GGGACATCCACAGTACAAACTGTCGCCAGAGGCTAATCTGATGGGTTTTGCCCACTATCTTGAAGCCCTCGACTTCCAAC
GCGAGATCGTCAAAATCCATACCATATTTGGCGGTAAGAACCCCCATCCTAACTGGATCGTCGGCGGGATGCCGTGCGCG
ATCAATCTCGATCAAAGCGGCGCGGTCGGGGCTATCAATATGGAGCGCCTCAACCTGGTGCAGTCGATCATCACCCGCAC
CGCCGACTTCATTAACAACGTGATGGTTCCGGACGCGCTGGCTATCGGCCAGTTCAATAAAGCATGGAGTCAAATTGGCA
CCGGTCTGTCGGATAAATGCGTGCTGAGTTACGGCGCATTTCCGGACATTGCCAACGACTTCAGCCAGCAAAGCCTGTTA
ATGCCGGGCGGCGCGGTGGTCAATGGCGATTTTAAAAATGTCATGCCGGTAGATTTGGCCGATCCGCAGCAGATACAGGA
GTTTGTGGATCACGCCTGGTACCGCTATCCGGACGATCGGCTGGGTCGTCATCCTTTTGACGGCATTACCGATCCCTGGT
ACAACCCGGGTGATGTCAAAGGCAGCGATACGCATATCCAGCAGCTCAACGAGCAGGAACGTTACTCCTGGATTAAAGCG
CCGCGCTGGCGGGGTCATGCCATGGAAGTCGGCCCGCTTGCCCGAACGCTTATCGCGTACCACAAAGGGGATGCCGCCAC
CATAGAGTCGGTAGATCGCATGATGTCCGCCCTTAAACTGCCGCTCTCCGGTATCCAGTCTACGCTTGGTCGTATTTTAT
GCCGCGCGCACGAAGCGCAATGGGCCGTCGGTAAGCTGCAGTATTTTTTCGACAGGCTTATGACCAACCTGAAAAACGGC
GACCTGGCCACCGCCAATACCGAGAAATGGGAACCGGCCAGTTGGCCGCAGCACTGCCGCGGCATCGGTTTTACCGAAGC
GCCTCGCGGAGCGTTGGGACACTGGGCGTCAATACGCGATCAGAAGATTGAACTCTATCAGTGCGTGGTCCCCACCACCT
GGAACGCCAGCCCACGCGATCCTAAAAAACAGATCGGCGCCTATGAAGCGGCATTGATGGGAACGCAAATGGCGATTCCC
GACCAACCGTTAGAAATCCTGAGGACGCTGCACAGCTTCGACCCCTGCCTCGCCTGTTCAACCCACGTGCTCGGCGACGA
CGGCAGCGAACTGATTGCCGTCCAGGTACGCTAA

Upstream 100 bases:

>100_bases
TTGGCGGTCACGCTGTCGCCAGCGCGCTCAACCAACGTAAACGCCACAAACAACAGTTAGCGCAAGCCGAACAACAGCCG
GACAATGAGGATAAACAGGC

Downstream 100 bases:

>100_bases
GCGTTAAGGAAAAGAACGATGACTGGAAAGCTATCTCCACGCGTCGGGGAGGCGCGTGATACTGCCGTCAGCCACTATGT
GTTTGAAGCGCCGGTACGCC

Product: hydrogenase 1 large subunit

Products: NA

Alternate protein names: HYD1; Membrane-bound hydrogenase 1 large subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MSNQYQTQGYTVNDAGRRLIVDPITRIEGHMRCEVNIDEQNVITNAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCT
GVHALASVYAIEDAIGIQVPDNANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLNALKADPRATSQLAQSLSAWPMSSP
GYFFDVQNRLKKFVDGGQLGIFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTIFGGKNPHPNWIVGGMPCA
INLDQSGAVGAINMERLNLVQSIITRTADFINNVMVPDALAIGQFNKAWSQIGTGLSDKCVLSYGAFPDIANDFSQQSLL
MPGGAVVNGDFKNVMPVDLADPQQIQEFVDHAWYRYPDDRLGRHPFDGITDPWYNPGDVKGSDTHIQQLNEQERYSWIKA
PRWRGHAMEVGPLARTLIAYHKGDAATIESVDRMMSALKLPLSGIQSTLGRILCRAHEAQWAVGKLQYFFDRLMTNLKNG
DLATANTEKWEPASWPQHCRGIGFTEAPRGALGHWASIRDQKIELYQCVVPTTWNASPRDPKKQIGAYEAALMGTQMAIP
DQPLEILRTLHSFDPCLACSTHVLGDDGSELIAVQVR

Sequences:

>Translated_597_residues
MSNQYQTQGYTVNDAGRRLIVDPITRIEGHMRCEVNIDEQNVITNAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCT
GVHALASVYAIEDAIGIQVPDNANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLNALKADPRATSQLAQSLSAWPMSSP
GYFFDVQNRLKKFVDGGQLGIFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTIFGGKNPHPNWIVGGMPCA
INLDQSGAVGAINMERLNLVQSIITRTADFINNVMVPDALAIGQFNKAWSQIGTGLSDKCVLSYGAFPDIANDFSQQSLL
MPGGAVVNGDFKNVMPVDLADPQQIQEFVDHAWYRYPDDRLGRHPFDGITDPWYNPGDVKGSDTHIQQLNEQERYSWIKA
PRWRGHAMEVGPLARTLIAYHKGDAATIESVDRMMSALKLPLSGIQSTLGRILCRAHEAQWAVGKLQYFFDRLMTNLKNG
DLATANTEKWEPASWPQHCRGIGFTEAPRGALGHWASIRDQKIELYQCVVPTTWNASPRDPKKQIGAYEAALMGTQMAIP
DQPLEILRTLHSFDPCLACSTHVLGDDGSELIAVQVR
>Mature_596_residues
SNQYQTQGYTVNDAGRRLIVDPITRIEGHMRCEVNIDEQNVITNAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTG
VHALASVYAIEDAIGIQVPDNANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLNALKADPRATSQLAQSLSAWPMSSPG
YFFDVQNRLKKFVDGGQLGIFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTIFGGKNPHPNWIVGGMPCAI
NLDQSGAVGAINMERLNLVQSIITRTADFINNVMVPDALAIGQFNKAWSQIGTGLSDKCVLSYGAFPDIANDFSQQSLLM
PGGAVVNGDFKNVMPVDLADPQQIQEFVDHAWYRYPDDRLGRHPFDGITDPWYNPGDVKGSDTHIQQLNEQERYSWIKAP
RWRGHAMEVGPLARTLIAYHKGDAATIESVDRMMSALKLPLSGIQSTLGRILCRAHEAQWAVGKLQYFFDRLMTNLKNGD
LATANTEKWEPASWPQHCRGIGFTEAPRGALGHWASIRDQKIELYQCVVPTTWNASPRDPKKQIGAYEAALMGTQMAIPD
QPLEILRTLHSFDPCLACSTHVLGDDGSELIAVQVR

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=597, Percent_Identity=90.9547738693467, Blast_Score=1166, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=591, Percent_Identity=42.3011844331641, Blast_Score=473, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 66557; Mature: 66425

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQYQTQGYTVNDAGRRLIVDPITRIEGHMRCEVNIDEQNVITNAVSCGTMFRGLEIIL
CCCCCCCCCEEECCCCCEEEECCHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHEE
QGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIQVPDNANIIRNIMLATLWCHDHL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHHHHHHHH
VHFYQLAGMDWIDVLNALKADPRATSQLAQSLSAWPMSSPGYFFDVQNRLKKFVDGGQLG
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCEE
IFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTIFGGKNPHPNWIVGGMPCA
EEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEECCCCEE
INLDQSGAVGAINMERLNLVQSIITRTADFINNVMVPDALAIGQFNKAWSQIGTGLSDKC
EECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHCCCCCCCE
VLSYGAFPDIANDFSQQSLLMPGGAVVNGDFKNVMPVDLADPQQIQEFVDHAWYRYPDDR
EEECCCCCHHHHHHHHCCEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
LGRHPFDGITDPWYNPGDVKGSDTHIQQLNEQERYSWIKAPRWRGHAMEVGPLARTLIAY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHH
HKGDAATIESVDRMMSALKLPLSGIQSTLGRILCRAHEAQWAVGKLQYFFDRLMTNLKNG
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLATANTEKWEPASWPQHCRGIGFTEAPRGALGHWASIRDQKIELYQCVVPTTWNASPRD
CEECCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
PKKQIGAYEAALMGTQMAIPDQPLEILRTLHSFDPCLACSTHVLGDDGSELIAVQVR
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHCCCEECCCCCCEEEEEEC
>Mature Secondary Structure 
SNQYQTQGYTVNDAGRRLIVDPITRIEGHMRCEVNIDEQNVITNAVSCGTMFRGLEIIL
CCCCCCCCEEECCCCCEEEECCHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHEE
QGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIQVPDNANIIRNIMLATLWCHDHL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHHHHHHHH
VHFYQLAGMDWIDVLNALKADPRATSQLAQSLSAWPMSSPGYFFDVQNRLKKFVDGGQLG
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCEE
IFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTIFGGKNPHPNWIVGGMPCA
EEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEECCCCEE
INLDQSGAVGAINMERLNLVQSIITRTADFINNVMVPDALAIGQFNKAWSQIGTGLSDKC
EECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHCCCCCCCE
VLSYGAFPDIANDFSQQSLLMPGGAVVNGDFKNVMPVDLADPQQIQEFVDHAWYRYPDDR
EEECCCCCHHHHHHHHCCEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
LGRHPFDGITDPWYNPGDVKGSDTHIQQLNEQERYSWIKAPRWRGHAMEVGPLARTLIAY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHH
HKGDAATIESVDRMMSALKLPLSGIQSTLGRILCRAHEAQWAVGKLQYFFDRLMTNLKNG
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLATANTEKWEPASWPQHCRGIGFTEAPRGALGHWASIRDQKIELYQCVVPTTWNASPRD
CEECCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
PKKQIGAYEAALMGTQMAIPDQPLEILRTLHSFDPCLACSTHVLGDDGSELIAVQVR
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHCCCEECCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA