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The map label for this gene is cysN

Identifier: 194737140

GI number: 194737140

Start: 2978280

End: 2979719

Strand: Reverse

Name: cysN

Synonym: SeSA_A3085

Alternate gene names: 194737140

Gene position: 2979719-2978280 (Counterclockwise)

Preceding gene: 194734255

Following gene: 194735437

Centisome position: 63.28

GC content: 55.14

Gene sequence:

>1440_bases
ATGAACACCATACTTGCACAACAAATCGCCAATGAAGGCGGCGTCGAAGCCTGGATGATGGCCCAACAGCACAAAAGCCT
GCTGCGTTTTTTAACCTGTGGCAGCGTTGATGACGGTAAAAGTACGCTGATAGGTCGTTTGCTGCACGATACCCTGCAAA
TTTATGAAGATCAGCTCTCTTCTCTGCATAATGACAGTAAACGTCATGGCACGCAGGGAGAAAAACTCGATCTGGCGCTG
CTGGTAGATGGGCTACAGGCCGAGCGTGAGCAGGGCATTACTATTGACGTCGCGTACCGCTATTTCTCCACGGAAAAACG
AAAATTTATTATCGCCGACACGCCGGGCCATGAACAATATACCCGTAATATGGCGACCGGGGCGTCCACCTGCGATCTGG
CGATCCTACTGATCGACGCGCGTAAAGGCGTGCTGGATCAGACGCGTCGCCATAGCTTTATCTCCACTCTGTTGGGGATC
AAACACCTGGTGGTGGCAATCAACAAAATGGATCTCGTTGACTACCGCGAAGAGACCTTCGCCCGTATTCGTGAAGATTA
CCTGACCTTTGCTGAACAGTTGCCGGGCGATCTGGATATTCGCTTTGTCCCGCTTTCGGCGCTGGAAGGGGATAATGTCG
CCGCGCAGAGCGCGAATATGCGCTGGTACAGCGGTCCGACGCTGCTGGAAGTCCTGGAAACGGTAGACATACAGCGTGCG
GTAGACCGTCAGCCGATGCGCTTTCCGGTACAATATGTCAATCGCCCGAACCTTGATTTTCGGGGATATGCCGGCACTCT
GGCGTCTGGTAGCGTCAAAGTCGGAGAGCGCATTAAAGTGCTGCCGTCCGGCGTGGAATCCAGCGTGGCGCGTATCGTCA
CTTTTGATGGCGATAAAGAAGAAGCCTGTGCGGGCGAAGCCATTACCCTGGTACTCAACGACGACATTGATATTAGCCGC
GGCGATCTGCTGCTGGCGGCAAACGAGACGCTGGCACCTGCGCGTCACGCCGCGATCGACGTGGTATGGATGGCGGAACA
GCCGCTGGCGCCGGGCCAAAGCTACGACGTTAAACTCGCGGGTAAGAAAACCCGCGCGCGTATCGAGGCTATTCGCTATC
AGATTGATATCAACAATCTGACCCAACGAGACGTCGAGAGCCTGCCGTTAAACGGTATTGGTCTGGTAGAGATGACATTC
GATGAGCCGCTCGCGCTGGATATTTATCAGCAAAATCCGGTGACGGGCGGGCTGATTTTTATCGACCGACTTTCTAACGT
CACCGTGGGCGCTGGCATGGTGCGGGAACTGGACGAACGCGGAGCGACGCCGCCCGTGGAATACAGTGCGTTTGAGCTGG
AGCTGAATGCGCTGGTACGCCGTCATTTTCCGCACTGGGACGCCCGCGATTTGCTGGGAGATAAACATGGCGCTGCATGA

Upstream 100 bases:

>100_bases
GGTTTCTACCACCAGCGAGCGCCAGGGAAGGATGATTGACCGCGATCAGGCGGGGTCGATGGAGCTTAAAAAACGTCAGG
GGTATTTCTAAGGAGCCGCC

Downstream 100 bases:

>100_bases
TGAGAACGTGGTCTGGCACTCTCATCCCGTTACTGTCGCCGCCCGTGAACAGCTCCACGGCCACCGTGGCGTGGTGCTGT
GGTTTACCGGACTCTCCGGT

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNTILAQQIANEGGVEAWMMAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTLQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYREETFARIREDYLTFAEQLPGDLDIRFVPLSALEGDNVAAQSANMRWYSGPTLLEVLETVDIQRA
VDRQPMRFPVQYVNRPNLDFRGYAGTLASGSVKVGERIKVLPSGVESSVARIVTFDGDKEEACAGEAITLVLNDDIDISR
GDLLLAANETLAPARHAAIDVVWMAEQPLAPGQSYDVKLAGKKTRARIEAIRYQIDINNLTQRDVESLPLNGIGLVEMTF
DEPLALDIYQQNPVTGGLIFIDRLSNVTVGAGMVRELDERGATPPVEYSAFELELNALVRRHFPHWDARDLLGDKHGAA

Sequences:

>Translated_479_residues
MNTILAQQIANEGGVEAWMMAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTLQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYREETFARIREDYLTFAEQLPGDLDIRFVPLSALEGDNVAAQSANMRWYSGPTLLEVLETVDIQRA
VDRQPMRFPVQYVNRPNLDFRGYAGTLASGSVKVGERIKVLPSGVESSVARIVTFDGDKEEACAGEAITLVLNDDIDISR
GDLLLAANETLAPARHAAIDVVWMAEQPLAPGQSYDVKLAGKKTRARIEAIRYQIDINNLTQRDVESLPLNGIGLVEMTF
DEPLALDIYQQNPVTGGLIFIDRLSNVTVGAGMVRELDERGATPPVEYSAFELELNALVRRHFPHWDARDLLGDKHGAA
>Mature_479_residues
MNTILAQQIANEGGVEAWMMAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTLQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYREETFARIREDYLTFAEQLPGDLDIRFVPLSALEGDNVAAQSANMRWYSGPTLLEVLETVDIQRA
VDRQPMRFPVQYVNRPNLDFRGYAGTLASGSVKVGERIKVLPSGVESSVARIVTFDGDKEEACAGEAITLVLNDDIDISR
GDLLLAANETLAPARHAAIDVVWMAEQPLAPGQSYDVKLAGKKTRARIEAIRYQIDINNLTQRDVESLPLNGIGLVEMTF
DEPLALDIYQQNPVTGGLIFIDRLSNVTVGAGMVRELDERGATPPVEYSAFELELNALVRRHFPHWDARDLLGDKHGAA

Specific function: May be the GTPase, regulating ATP sulfurylase activity

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily

Homologues:

Organism=Homo sapiens, GI223555963, Length=438, Percent_Identity=30.1369863013699, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI5729864, Length=438, Percent_Identity=30.1369863013699, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI194018522, Length=336, Percent_Identity=31.547619047619, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI194018520, Length=318, Percent_Identity=32.3899371069182, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI194097354, Length=318, Percent_Identity=32.3899371069182, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=27.1111111111111, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI46094014, Length=336, Percent_Identity=30.6547619047619, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=26.5625, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI34147630, Length=346, Percent_Identity=27.7456647398844, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1789108, Length=475, Percent_Identity=87.7894736842105, Blast_Score=863, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=422, Percent_Identity=26.5402843601896, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790412, Length=422, Percent_Identity=26.5402843601896, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367247, Length=354, Percent_Identity=25.9887005649718, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115532067, Length=427, Percent_Identity=25.9953161592506, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI115532065, Length=427, Percent_Identity=25.9953161592506, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI32566629, Length=427, Percent_Identity=27.6346604215457, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17552884, Length=441, Percent_Identity=29.0249433106576, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=441, Percent_Identity=29.0249433106576, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=27.6497695852535, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25141371, Length=232, Percent_Identity=27.5862068965517, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=338, Percent_Identity=29.2899408284024, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.9565217391304, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322937, Length=446, Percent_Identity=28.2511210762332, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325337, Length=324, Percent_Identity=30.2469135802469, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=324, Percent_Identity=30.2469135802469, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6320377, Length=430, Percent_Identity=26.9767441860465, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6324761, Length=299, Percent_Identity=27.4247491638796, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=149, Percent_Identity=34.2281879194631, Blast_Score=63, Evalue=1e-10,
Organism=Drosophila melanogaster, GI45550900, Length=434, Percent_Identity=28.5714285714286, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24652838, Length=439, Percent_Identity=26.1958997722096, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17137572, Length=439, Percent_Identity=26.1958997722096, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45553807, Length=439, Percent_Identity=26.1958997722096, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI45553816, Length=439, Percent_Identity=26.1958997722096, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24651721, Length=439, Percent_Identity=26.1958997722096, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI17864154, Length=439, Percent_Identity=26.1958997722096, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI17137380, Length=315, Percent_Identity=29.8412698412698, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281363316, Length=305, Percent_Identity=29.1803278688525, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17864358, Length=305, Percent_Identity=29.1803278688525, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19921738, Length=307, Percent_Identity=28.0130293159609, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSN_SALA4 (B5F415)

Other databases:

- EMBL:   CP001138
- RefSeq:   YP_002147831.1
- GeneID:   6795708
- GenomeReviews:   CP001138_GR
- KEGG:   sea:SeAg_B3059
- HOGENOM:   HBG307581
- OMA:   SAQIYED
- ProtClustDB:   PRK05124
- HAMAP:   MF_00062
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR02034
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: =2.7.7.4

Molecular weight: Translated: 53153; Mature: 53153

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTILAQQIANEGGVEAWMMAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTLQIYEDQLS
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYREETF
HHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARIREDYLTFAEQLPGDLDIRFVPLSALEGDNVAAQSANMRWYSGPTLLEVLETVDIQRA
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCEEECCCHHHHHHHHHHHHHH
VDRQPMRFPVQYVNRPNLDFRGYAGTLASGSVKVGERIKVLPSGVESSVARIVTFDGDKE
HCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEECCCEEEECCCCCCCCEEEEEEECCCCC
EACAGEAITLVLNDDIDISRGDLLLAANETLAPARHAAIDVVWMAEQPLAPGQSYDVKLA
CCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEC
GKKTRARIEAIRYQIDINNLTQRDVESLPLNGIGLVEMTFDEPLALDIYQQNPVTGGLIF
CCHHHHHEEEEEEEEECCCCCHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCCCCEEE
IDRLSNVTVGAGMVRELDERGATPPVEYSAFELELNALVRRHFPHWDARDLLGDKHGAA
EECCCCCEECHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MNTILAQQIANEGGVEAWMMAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTLQIYEDQLS
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYREETF
HHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARIREDYLTFAEQLPGDLDIRFVPLSALEGDNVAAQSANMRWYSGPTLLEVLETVDIQRA
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCEEECCCHHHHHHHHHHHHHH
VDRQPMRFPVQYVNRPNLDFRGYAGTLASGSVKVGERIKVLPSGVESSVARIVTFDGDKE
HCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEECCCEEEECCCCCCCCEEEEEEECCCCC
EACAGEAITLVLNDDIDISRGDLLLAANETLAPARHAAIDVVWMAEQPLAPGQSYDVKLA
CCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEC
GKKTRARIEAIRYQIDINNLTQRDVESLPLNGIGLVEMTFDEPLALDIYQQNPVTGGLIF
CCHHHHHEEEEEEEEECCCCCHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCCCCEEE
IDRLSNVTVGAGMVRELDERGATPPVEYSAFELELNALVRRHFPHWDARDLLGDKHGAA
EECCCCCEECHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA