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The map label for this gene is rutR [H]

Identifier: 194737095

GI number: 194737095

Start: 1165182

End: 1165820

Strand: Direct

Name: rutR [H]

Synonym: SeSA_A1186

Alternate gene names: 194737095

Gene position: 1165182-1165820 (Clockwise)

Preceding gene: 194738107

Following gene: 194733910

Centisome position: 24.74

GC content: 55.24

Gene sequence:

>639_bases
ATGTCGCAACGCACAGAGAAAAAAATCGGGAAACGCTCGCAGGCCACCGGTGCAAAACGGCAGCTTATCTTAACCGCCGC
GCTTGCTGTTTTTTCCCAGTATGGCATTCATGGCGCGCGTCTTGAGCAGGTCGCCGAGCGGGCAGGCGTCTCCAAAACCA
ATCTGCTTTATTATTATCCCTCGAAAGAGGCGCTGTATGTCGCGGTAATGCGACAGATCCTGGATGTCTGGCTGGCGCCG
CTCAAGGCGTTTCGCGCAGAATTTTCCCCTCTGGAGGCCATCAAAGAGTATATCCGTCTCAAGCTGGAGGTTTCGCGTGA
TTATCCGCAGGCGTCGCGGCTCTTCTGCATGGAGATGCTGGCGGGCGCGCCGCTCTTAATGGAGGAACTGACCGGCGATC
TAAAAGCGTTGATAGATGAAAAATCCGCGCTGATTGCCGGATGGGTGCACAGCGGGAAACTGGCGCCTGTCTCCCCGCAC
CATTTGATCTTCATGATTTGGGCCGCCACGCAACATTACGCCGATTTTGCCCCTCAGGTTGAGGCGGTAACCGGCGCGAC
GCTTCGCGATGAAGCCTTTTTCAACCAAACGGTCGAAAGCGTTCAGCGCATTATTATTGAAGGGATTCGCGTGCGTTAA

Upstream 100 bases:

>100_bases
CTAAAAAGCGTCATGCGCCACCGCCGCCGGATTTCCGGCGGTTTTTTTGTTTATAAGCCGGATTTGGTATGCTTGCGTCC
TGACAAAAAGGAGAGGGCGC

Downstream 100 bases:

>100_bases
CCGGCTGGCGGCAGTGGGCAGCTCAAATCCCCCTCTTCACATTGCAGCAGCGAGGCTAAAAACGCTTCGCGTTCGTTCGT
TTTATCGGTCAGGCACTGGC

Product: HTH-type transcriptional regulator RutR

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSQRTEKKIGKRSQATGAKRQLILTAALAVFSQYGIHGARLEQVAERAGVSKTNLLYYYPSKEALYVAVMRQILDVWLAP
LKAFRAEFSPLEAIKEYIRLKLEVSRDYPQASRLFCMEMLAGAPLLMEELTGDLKALIDEKSALIAGWVHSGKLAPVSPH
HLIFMIWAATQHYADFAPQVEAVTGATLRDEAFFNQTVESVQRIIIEGIRVR

Sequences:

>Translated_212_residues
MSQRTEKKIGKRSQATGAKRQLILTAALAVFSQYGIHGARLEQVAERAGVSKTNLLYYYPSKEALYVAVMRQILDVWLAP
LKAFRAEFSPLEAIKEYIRLKLEVSRDYPQASRLFCMEMLAGAPLLMEELTGDLKALIDEKSALIAGWVHSGKLAPVSPH
HLIFMIWAATQHYADFAPQVEAVTGATLRDEAFFNQTVESVQRIIIEGIRVR
>Mature_211_residues
SQRTEKKIGKRSQATGAKRQLILTAALAVFSQYGIHGARLEQVAERAGVSKTNLLYYYPSKEALYVAVMRQILDVWLAPL
KAFRAEFSPLEAIKEYIRLKLEVSRDYPQASRLFCMEMLAGAPLLMEELTGDLKALIDEKSALIAGWVHSGKLAPVSPHH
LIFMIWAATQHYADFAPQVEAVTGATLRDEAFFNQTVESVQRIIIEGIRVR

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=212, Percent_Identity=82.5471698113208, Blast_Score=369, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 23792; Mature: 23661

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRTEKKIGKRSQATGAKRQLILTAALAVFSQYGIHGARLEQVAERAGVSKTNLLYYYP
CCCHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEC
SKEALYVAVMRQILDVWLAPLKAFRAEFSPLEAIKEYIRLKLEVSRDYPQASRLFCMEML
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AGAPLLMEELTGDLKALIDEKSALIAGWVHSGKLAPVSPHHLIFMIWAATQHYADFAPQV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCH
EAVTGATLRDEAFFNQTVESVQRIIIEGIRVR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQRTEKKIGKRSQATGAKRQLILTAALAVFSQYGIHGARLEQVAERAGVSKTNLLYYYP
CCHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEC
SKEALYVAVMRQILDVWLAPLKAFRAEFSPLEAIKEYIRLKLEVSRDYPQASRLFCMEML
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AGAPLLMEELTGDLKALIDEKSALIAGWVHSGKLAPVSPHHLIFMIWAATQHYADFAPQV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCH
EAVTGATLRDEAFFNQTVESVQRIIIEGIRVR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]