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The map label for this gene is fixJ [H]

Identifier: 194736865

GI number: 194736865

Start: 1437052

End: 1437690

Strand: Direct

Name: fixJ [H]

Synonym: SeSA_A1484

Alternate gene names: 194736865

Gene position: 1437052-1437690 (Clockwise)

Preceding gene: 194735596

Following gene: 194734444

Centisome position: 30.52

GC content: 55.24

Gene sequence:

>639_bases
ATGAACATGGGGGCAGGGATGGCGACAATTCATCTACTGGATGATGATACGGCGGTCACTAACGCGTGCGCGTTTTTACT
GGAAAGTCTGGGATATGACGTAAAATGCTGGACGCAGGGGGCGGATTTTTTGGCGCAGGCCAGTCTGTATCAGGCCGGAG
TCGTATTACTGGATATGCGAATGCCGGTACTGGATGGGCAGGGCGTTCATGATGCGTTGCGCCAGTGCGGAAGTACTCTG
GCGGTTGTTTTTCTTACCGGGCATGGCGATGTGCCGATGGCCGTGGAGCAGATGAAACGCGGCGCCGTCGATTTTCTGCA
AAAACCGGTATCGGTAAAACCGCTACAGGCGGCGCTGGAGCGTGCGCTGACGGTTTCATCGGCAGCGGTGGCGCGCCGTG
AGATTATACTGTGTTACCAGCAGTTGACGCCGAAAGAGCGTGAGCTGGCCAGCCTGGTGGTAAAAGGATTTATGAACCGT
GAAATTGCGGAAGCGATGAATATCGCGGTGCGCACCGTAGAGGTGCACCGCGCCAGAGTCATGGAAAAAATGCAGGCCGG
TAGCCTGGCGGAACTGATTAGGCGTTTCGAAAAAATGGCCTCGCCAGAGACCAGAATACGAACAACGTATGAGCCATGA

Upstream 100 bases:

>100_bases
TGCCAGCGGCTGGTGCGGTATGGGCGGGGCGATATCAGCATCAGGAACCAGACCGCGCCGGACGGTCTGTCGGGAACGGT
GGTCACGATACATTTCTTAC

Downstream 100 bases:

>100_bases
ATAATAAACTGCGTTATTTCTTTGATTTGCATCAAATTTTGTTTAATTGAGAAGACTGTTATATTCTGTTGGTTTGAGCT
GGCAACGAGTAAAACGTATG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNMGAGMATIHLLDDDTAVTNACAFLLESLGYDVKCWTQGADFLAQASLYQAGVVLLDMRMPVLDGQGVHDALRQCGSTL
AVVFLTGHGDVPMAVEQMKRGAVDFLQKPVSVKPLQAALERALTVSSAAVARREIILCYQQLTPKERELASLVVKGFMNR
EIAEAMNIAVRTVEVHRARVMEKMQAGSLAELIRRFEKMASPETRIRTTYEP

Sequences:

>Translated_212_residues
MNMGAGMATIHLLDDDTAVTNACAFLLESLGYDVKCWTQGADFLAQASLYQAGVVLLDMRMPVLDGQGVHDALRQCGSTL
AVVFLTGHGDVPMAVEQMKRGAVDFLQKPVSVKPLQAALERALTVSSAAVARREIILCYQQLTPKERELASLVVKGFMNR
EIAEAMNIAVRTVEVHRARVMEKMQAGSLAELIRRFEKMASPETRIRTTYEP
>Mature_212_residues
MNMGAGMATIHLLDDDTAVTNACAFLLESLGYDVKCWTQGADFLAQASLYQAGVVLLDMRMPVLDGQGVHDALRQCGSTL
AVVFLTGHGDVPMAVEQMKRGAVDFLQKPVSVKPLQAALERALTVSSAAVARREIILCYQQLTPKERELASLVVKGFMNR
EIAEAMNIAVRTVEVHRARVMEKMQAGSLAELIRRFEKMASPETRIRTTYEP

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=34.2105263157895, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790102, Length=197, Percent_Identity=31.9796954314721, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788905, Length=155, Percent_Identity=30.9677419354839, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790299, Length=115, Percent_Identity=29.5652173913043, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23283; Mature: 23283

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMGAGMATIHLLDDDTAVTNACAFLLESLGYDVKCWTQGADFLAQASLYQAGVVLLDMR
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCHHEEECC
MPVLDGQGVHDALRQCGSTLAVVFLTGHGDVPMAVEQMKRGAVDFLQKPVSVKPLQAALE
CCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
RALTVSSAAVARREIILCYQQLTPKERELASLVVKGFMNREIAEAMNIAVRTVEVHRARV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEKMQAGSLAELIRRFEKMASPETRIRTTYEP
HHHHCCCCHHHHHHHHHHHCCCCHHCCCCCCC
>Mature Secondary Structure
MNMGAGMATIHLLDDDTAVTNACAFLLESLGYDVKCWTQGADFLAQASLYQAGVVLLDMR
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCHHEEECC
MPVLDGQGVHDALRQCGSTLAVVFLTGHGDVPMAVEQMKRGAVDFLQKPVSVKPLQAALE
CCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
RALTVSSAAVARREIILCYQQLTPKERELASLVVKGFMNREIAEAMNIAVRTVEVHRARV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEKMQAGSLAELIRRFEKMASPETRIRTTYEP
HHHHCCCCHHHHHHHHHHHCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]