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The map label for this gene is fixL [H]

Identifier: 194735596

GI number: 194735596

Start: 1435347

End: 1437095

Strand: Direct

Name: fixL [H]

Synonym: SeSA_A1483

Alternate gene names: 194735596

Gene position: 1435347-1437095 (Clockwise)

Preceding gene: 194738000

Following gene: 194736865

Centisome position: 30.48

GC content: 56.55

Gene sequence:

>1749_bases
GTGTTGGCGGCAGTAGGGCTACTTTGTCATCGCGCGTGGGCAGGGACGTGGAATATCGGTATTTTGGCCATGCGCGGTGA
GGCGTCTACGCGTAGCCACTGGCAACCGTTGGCAAAGACACTAAGCCAACAGCTTCCAGGCGAAACCTTTCACATCCAGC
CGCTGGATCTGCATCAAATGCAGGAGGCCGTTAACCAGGGAACCGTGCAGTTTGTGATAACCAACCCGGCACAATTTGTC
CAACTGAACAGCCATGCGCCGCTGCGCTGGTTAGCTTCCCTGCGTTCCACGCGCGATGGGAAAGCGGTGAGTAATGTTAT
TGGCAGCGTGATTTTGACCCGGCGCGATAGCGGCATCACCACGGCGCATGATCTCATCGGTAAGACAGTCGGCGCGATTG
ATGCTCAGGCGTTTGGCGGCTATTTATTAGGCTATAAAGCGCTCAGCGACGCGGGCTTACGCCCGGAGCGCGATTTTCAT
CTTCGTTTTACCGGATTTCCTGGCGATGCCTTAGTCTATATGCTGCGCGAAAAAGCGGTGCAGGCGGCAATTGTGCCAGT
GTGTCTGTTAGAAAATATGGATCAGGAAGGATTGATTAATAAAAAGGACTTTATCGCGCTGCTTTCCCGACCGACGCCCC
TGCCTTGCTTAACCAGTACGCCGTTATATCCTGACTGGTCGTTCGCGGCGCTACCTGCGGTAAGCGATGCGCTGGCGGAT
CGCGTAACGCGAGCGCTATTCAACGCGCCCGCCGCCGCGTCATTTCACTGGGGCGCGCCTGCGTCCACCAGTCAGGTGGA
AGCGTTGCTGCGTGATGTTCGTCAGCACCCTCAGCAGCGTCGACTGTGGCTGGATGTCAAAAGTTGGTTAATCCAGCACC
AGCTAATAGTCGGCGGCGTGATTCTGGCGTTCCTGTTGCTCACGCTCAATTATATTTGGGTCATGCTGCTGGTGCGTCGA
CGCGGAAAGCAACTGGAACGTAATAGCGTAGTTCTTCATCAGCATGAGCGGGCGCTGGAAACCGCCCGGCAAATGAGCGT
GTTGGGTGAAATGACCTCCGGGTTTGCCCATGAGCTTAATCAGCCGCTTTCCGCGATTCGACATTATGCCCAGGGGTGCC
TGATTCGACTGCGCGCTGCAGATGAACAGCATCCCTTGCTGCCGGCGCTGGAGCAGATTGACCAGCAGGCGCAACGCGGT
GCGGATACTTTGCGTAACCTGCGTCACTGGGTTAGCCAGGCGCAGGGCAACCCGGTGCTAACCGAAGCGTGGAAGGCTAT
AGCCATTCGCGAGGCGATTGATCATGTCTGGCAATTGTTGCGTATGGCGCAACAGTTTCCGACAGTGAGTCTGCATACCG
AGGTTAGCGCTGCGCTGCGCGTAACGCTGCCGTCAATGCTGCTGGAACAGGTGCTGGCGAATATCATTCTTAATGCGGCT
CAGGCGGGCGCCACCCATTTATGGGTCATTGCTGAACGCACTGAAAACGGCATCAGTATCGTTTTACAGGATAACGCCGG
GGGAATCGACGAGGCGCTATTACGTCAGGCGTTTCAGCCGTTTATGACCACCCGCAAAGAGGGGATGGGCTTAGGGCTGG
CGATTTGCCAGCGGCTGGTGCGGTATGGGCGGGGCGATATCAGCATCAGGAACCAGACCGCGCCGGACGGTCTGTCGGGA
ACGGTGGTCACGATACATTTCTTACATGAACATGGGGGCAGGGATGGCGACAATTCATCTACTGGATGA

Upstream 100 bases:

>100_bases
CTCTATTGTGGTTAACCACATACCCGGCTTGTTGTTGATCTAAAACAACATAATTGACAGGGATATTGGGGTGAGAGGTA
AAACCGTAAGGCGCCTGGCG

Downstream 100 bases:

>100_bases
TGATACGGCGGTCACTAACGCGTGCGCGTTTTTACTGGAAAGTCTGGGATATGACGTAAAATGCTGGACGCAGGGGGCGG
ATTTTTTGGCGCAGGCCAGT

Product: sensor-kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MLAAVGLLCHRAWAGTWNIGILAMRGEASTRSHWQPLAKTLSQQLPGETFHIQPLDLHQMQEAVNQGTVQFVITNPAQFV
QLNSHAPLRWLASLRSTRDGKAVSNVIGSVILTRRDSGITTAHDLIGKTVGAIDAQAFGGYLLGYKALSDAGLRPERDFH
LRFTGFPGDALVYMLREKAVQAAIVPVCLLENMDQEGLINKKDFIALLSRPTPLPCLTSTPLYPDWSFAALPAVSDALAD
RVTRALFNAPAAASFHWGAPASTSQVEALLRDVRQHPQQRRLWLDVKSWLIQHQLIVGGVILAFLLLTLNYIWVMLLVRR
RGKQLERNSVVLHQHERALETARQMSVLGEMTSGFAHELNQPLSAIRHYAQGCLIRLRAADEQHPLLPALEQIDQQAQRG
ADTLRNLRHWVSQAQGNPVLTEAWKAIAIREAIDHVWQLLRMAQQFPTVSLHTEVSAALRVTLPSMLLEQVLANIILNAA
QAGATHLWVIAERTENGISIVLQDNAGGIDEALLRQAFQPFMTTRKEGMGLGLAICQRLVRYGRGDISIRNQTAPDGLSG
TVVTIHFLHEHGGRDGDNSSTG

Sequences:

>Translated_582_residues
MLAAVGLLCHRAWAGTWNIGILAMRGEASTRSHWQPLAKTLSQQLPGETFHIQPLDLHQMQEAVNQGTVQFVITNPAQFV
QLNSHAPLRWLASLRSTRDGKAVSNVIGSVILTRRDSGITTAHDLIGKTVGAIDAQAFGGYLLGYKALSDAGLRPERDFH
LRFTGFPGDALVYMLREKAVQAAIVPVCLLENMDQEGLINKKDFIALLSRPTPLPCLTSTPLYPDWSFAALPAVSDALAD
RVTRALFNAPAAASFHWGAPASTSQVEALLRDVRQHPQQRRLWLDVKSWLIQHQLIVGGVILAFLLLTLNYIWVMLLVRR
RGKQLERNSVVLHQHERALETARQMSVLGEMTSGFAHELNQPLSAIRHYAQGCLIRLRAADEQHPLLPALEQIDQQAQRG
ADTLRNLRHWVSQAQGNPVLTEAWKAIAIREAIDHVWQLLRMAQQFPTVSLHTEVSAALRVTLPSMLLEQVLANIILNAA
QAGATHLWVIAERTENGISIVLQDNAGGIDEALLRQAFQPFMTTRKEGMGLGLAICQRLVRYGRGDISIRNQTAPDGLSG
TVVTIHFLHEHGGRDGDNSSTG
>Mature_582_residues
MLAAVGLLCHRAWAGTWNIGILAMRGEASTRSHWQPLAKTLSQQLPGETFHIQPLDLHQMQEAVNQGTVQFVITNPAQFV
QLNSHAPLRWLASLRSTRDGKAVSNVIGSVILTRRDSGITTAHDLIGKTVGAIDAQAFGGYLLGYKALSDAGLRPERDFH
LRFTGFPGDALVYMLREKAVQAAIVPVCLLENMDQEGLINKKDFIALLSRPTPLPCLTSTPLYPDWSFAALPAVSDALAD
RVTRALFNAPAAASFHWGAPASTSQVEALLRDVRQHPQQRRLWLDVKSWLIQHQLIVGGVILAFLLLTLNYIWVMLLVRR
RGKQLERNSVVLHQHERALETARQMSVLGEMTSGFAHELNQPLSAIRHYAQGCLIRLRAADEQHPLLPALEQIDQQAQRG
ADTLRNLRHWVSQAQGNPVLTEAWKAIAIREAIDHVWQLLRMAQQFPTVSLHTEVSAALRVTLPSMLLEQVLANIILNAA
QAGATHLWVIAERTENGISIVLQDNAGGIDEALLRQAFQPFMTTRKEGMGLGLAICQRLVRYGRGDISIRNQTAPDGLSG
TVVTIHFLHEHGGRDGDNSSTG

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=225, Percent_Identity=30.2222222222222, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1790436, Length=260, Percent_Identity=28.8461538461538, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64225; Mature: 64225

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAAVGLLCHRAWAGTWNIGILAMRGEASTRSHWQPLAKTLSQQLPGETFHIQPLDLHQM
CHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHH
QEAVNQGTVQFVITNPAQFVQLNSHAPLRWLASLRSTRDGKAVSNVIGSVILTRRDSGIT
HHHHCCCCEEEEEECCHHEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCC
TAHDLIGKTVGAIDAQAFGGYLLGYKALSDAGLRPERDFHLRFTGFPGDALVYMLREKAV
HHHHHHHHHHHHCHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHH
QAAIVPVCLLENMDQEGLINKKDFIALLSRPTPLPCLTSTPLYPDWSFAALPAVSDALAD
HHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RVTRALFNAPAAASFHWGAPASTSQVEALLRDVRQHPQQRRLWLDVKSWLIQHQLIVGGV
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
ILAFLLLTLNYIWVMLLVRRRGKQLERNSVVLHQHERALETARQMSVLGEMTSGFAHELN
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QPLSAIRHYAQGCLIRLRAADEQHPLLPALEQIDQQAQRGADTLRNLRHWVSQAQGNPVL
HHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
TEAWKAIAIREAIDHVWQLLRMAQQFPTVSLHTEVSAALRVTLPSMLLEQVLANIILNAA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAGATHLWVIAERTENGISIVLQDNAGGIDEALLRQAFQPFMTTRKEGMGLGLAICQRLV
HCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RYGRGDISIRNQTAPDGLSGTVVTIHFLHEHGGRDGDNSSTG
HCCCCCEEECCCCCCCCCCCCEEEEEEEHHCCCCCCCCCCCC
>Mature Secondary Structure
MLAAVGLLCHRAWAGTWNIGILAMRGEASTRSHWQPLAKTLSQQLPGETFHIQPLDLHQM
CHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHH
QEAVNQGTVQFVITNPAQFVQLNSHAPLRWLASLRSTRDGKAVSNVIGSVILTRRDSGIT
HHHHCCCCEEEEEECCHHEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCC
TAHDLIGKTVGAIDAQAFGGYLLGYKALSDAGLRPERDFHLRFTGFPGDALVYMLREKAV
HHHHHHHHHHHHCHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHH
QAAIVPVCLLENMDQEGLINKKDFIALLSRPTPLPCLTSTPLYPDWSFAALPAVSDALAD
HHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RVTRALFNAPAAASFHWGAPASTSQVEALLRDVRQHPQQRRLWLDVKSWLIQHQLIVGGV
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
ILAFLLLTLNYIWVMLLVRRRGKQLERNSVVLHQHERALETARQMSVLGEMTSGFAHELN
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QPLSAIRHYAQGCLIRLRAADEQHPLLPALEQIDQQAQRGADTLRNLRHWVSQAQGNPVL
HHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
TEAWKAIAIREAIDHVWQLLRMAQQFPTVSLHTEVSAALRVTLPSMLLEQVLANIILNAA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAGATHLWVIAERTENGISIVLQDNAGGIDEALLRQAFQPFMTTRKEGMGLGLAICQRLV
HCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RYGRGDISIRNQTAPDGLSGTVVTIHFLHEHGGRDGDNSSTG
HCCCCCEEECCCCCCCCCCCCEEEEEEEHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 8432704 [H]