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The map label for this gene is nlpE [H]

Identifier: 194735528

GI number: 194735528

Start: 284111

End: 284812

Strand: Direct

Name: nlpE [H]

Synonym: SeSA_A0268

Alternate gene names: 194735528

Gene position: 284111-284812 (Clockwise)

Preceding gene: 194736606

Following gene: 194736696

Centisome position: 6.03

GC content: 54.99

Gene sequence:

>702_bases
ATGGTGAGAACAGCAATATTGTCAGTAGCGGCAGCCTGTACGCTATTTGCATTGATAGGGTGTAATAACCGTGCGGAAGT
TGACGCCCTGCAACCTGCGCAGGCGGCGGAATTAAAACCCATGCAGCAAAGCTGGCGCGGCGTGCTGCCCTGTGCGGATT
GTGAAGGTATTGAAACGTCACTGTTTCTGGAAAAAGACGGCACATGGGTGATGAACGAGCGCTATCAGGGCGTGCGCGAG
GAACCTTCCTCTTTTGCGTCATACGGTACCTGGGCGCGGACGGCGGATAAACTGGTGTTAACCGACAGCAATGGCGAGAA
ATCGTATTATCGCGCGAAAGGCAATGCGCTGGAGATGCTCGACAGAGAAGGCAATCCGGTGGCTTCGCAGCTTAATTATA
CGCTTGTGCCTGTTACCGCCAGCCTGCCGGTAACGCCAATGCCGTTGCGTGGTATGTATGTATACAGGGCCGATGCGGCG
ACCTTTACCGATTGTGCGACCGGAAAACGGCTGCCGGTCGCCAGTAACGCCCAGCTTGAGCGCGGCTATCTGACGGCGAA
AGGTGAAGCGGAAAAACCGGTGCTGCTGACGGTAGAAGGGCATTTTGTGTTTGCCGCGAACCCGGACACGGGCGAGCCGG
TAAAAATGCTAATAGCCGATAAAAACGCGAAGTTTGCGCCGGGTAAAGATTGCACTCACTAA

Upstream 100 bases:

>100_bases
TTGTCCTCAAAGGCGCAAAAATCTTCTGTAAAGGCGCTTCGCGGCAAAGTTCGTCGTCCACTGGATTGACGGGCAGGAAT
AGCAATTTCATAAGGATTTT

Downstream 100 bases:

>100_bases
TAAAAGGCCTCGCAGGATGCGAGGCCTTTACATATCTGCCGGGTGGCGCCGGGCAATCCCAACAAAATGTCGTTTAGCCT
TTAATCGCCTTGACCAGATA

Product: lipoprotein involved with copper homeostasis and adhesion

Products: NA

Alternate protein names: Copper homeostasis protein CutF [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MVRTAILSVAAACTLFALIGCNNRAEVDALQPAQAAELKPMQQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYQGVRE
EPSSFASYGTWARTADKLVLTDSNGEKSYYRAKGNALEMLDREGNPVASQLNYTLVPVTASLPVTPMPLRGMYVYRADAA
TFTDCATGKRLPVASNAQLERGYLTAKGEAEKPVLLTVEGHFVFAANPDTGEPVKMLIADKNAKFAPGKDCTH

Sequences:

>Translated_233_residues
MVRTAILSVAAACTLFALIGCNNRAEVDALQPAQAAELKPMQQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYQGVRE
EPSSFASYGTWARTADKLVLTDSNGEKSYYRAKGNALEMLDREGNPVASQLNYTLVPVTASLPVTPMPLRGMYVYRADAA
TFTDCATGKRLPVASNAQLERGYLTAKGEAEKPVLLTVEGHFVFAANPDTGEPVKMLIADKNAKFAPGKDCTH
>Mature_233_residues
MVRTAILSVAAACTLFALIGCNNRAEVDALQPAQAAELKPMQQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYQGVRE
EPSSFASYGTWARTADKLVLTDSNGEKSYYRAKGNALEMLDREGNPVASQLNYTLVPVTASLPVTPMPLRGMYVYRADAA
TFTDCATGKRLPVASNAQLERGYLTAKGEAEKPVLLTVEGHFVFAANPDTGEPVKMLIADKNAKFAPGKDCTH

Specific function: Involved in copper homeostasis. Could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. When overproduced induces degP through the activation of the two-component system CpxA/CpxR [H]

COG id: COG3015

COG function: function code MP; Uncharacterized lipoprotein NlpE involved in copper resistance

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786390, Length=232, Percent_Identity=75.8620689655172, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007298 [H]

Pfam domain/function: PF04170 NlpE [H]

EC number: NA

Molecular weight: Translated: 25286; Mature: 25286

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00283 SOYBEAN_KUNITZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRTAILSVAAACTLFALIGCNNRAEVDALQPAQAAELKPMQQSWRGVLPCADCEGIETS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHCCCCCCCCCCCCCCEE
LFLEKDGTWVMNERYQGVREEPSSFASYGTWARTADKLVLTDSNGEKSYYRAKGNALEML
EEEECCCCEEECCCCCCCCCCCHHHHHCCCHHHCCCEEEEECCCCCCCEEEECCCEEEEC
DREGNPVASQLNYTLVPVTASLPVTPMPLRGMYVYRADAATFTDCATGKRLPVASNAQLE
CCCCCCCHHHCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHCCCCCCCCCCCCCEEE
RGYLTAKGEAEKPVLLTVEGHFVFAANPDTGEPVKMLIADKNAKFAPGKDCTH
CCEEEECCCCCCCEEEEEECCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MVRTAILSVAAACTLFALIGCNNRAEVDALQPAQAAELKPMQQSWRGVLPCADCEGIETS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHCCCCCCCCCCCCCCEE
LFLEKDGTWVMNERYQGVREEPSSFASYGTWARTADKLVLTDSNGEKSYYRAKGNALEML
EEEECCCCEEECCCCCCCCCCCHHHHHCCCHHHCCCEEEEECCCCCCCEEEECCCEEEEC
DREGNPVASQLNYTLVPVTASLPVTPMPLRGMYVYRADAATFTDCATGKRLPVASNAQLE
CCCCCCCHHHCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHCCCCCCCCCCCCCEEE
RGYLTAKGEAEKPVLLTVEGHFVFAANPDTGEPVKMLIADKNAKFAPGKDCTH
CCEEEECCCCCCCEEEEEECCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7635808; 7635807; 9278503 [H]