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The map label for this gene is fumA [H]

Identifier: 194735303

GI number: 194735303

Start: 1509638

End: 1511284

Strand: Direct

Name: fumA [H]

Synonym: SeSA_A1567

Alternate gene names: 194735303

Gene position: 1509638-1511284 (Clockwise)

Preceding gene: 194737753

Following gene: 194734009

Centisome position: 32.06

GC content: 54.71

Gene sequence:

>1647_bases
ATGTCAAACAAACCCTTCTTTTATCAAGACCCTTTTCCCCTCAAAAAGGACGATACCGAGTACTACTTGTTGACCAGCGA
ACATGTTTCCGTCGCCGAATTCGAAGGACAAGAGATCCTGAAAGTCGCGCCGGAAGCGCTAACGCTGTTGGCGCGTCAGG
CCTTCCACGATGCGTCTTTTATGCTACGTCCCGCGCATCAGCAGCAGGTGGCGGATATTCTGCGCGATCCGCAGGCCAGT
GAAAACGACAAATATGTCGCGCTGCAATTCCTGCGAAACTCCGATATCGCGGCCAAGGGCGTGTTGCCCACCTGCCAGGA
TACCGGTACGGCCATTATTGTCGGCAAAAAAGGCCAACGCGTCTGGACTGGTGGAGGTGATGAAGCGGCGCTGGCTCGCG
GCGTATATAACACTTATATCGAAGATAACCTGCGCTATTCGCAAAACGCGGCGCTGGATATGTACAAAGAGGTCAATACC
GGTACTAACCTGCCGGCGCAGATCGATCTCTACAGCGTCGATGGCGATGAATATAAATTCCTCTGTATCGCTAAAGGCGG
CGGATCGGCGAATAAAACCTACCTCTATCAGGAGACAAAAGCGCTTCTGACGCCGGGTAAGCTCAAAAATTATCTCGTTG
ATAAGATGCGTACGCTGGGCACCGCCGCCTGTCCGCCGTATCACATTGCGTTTGTCATTGGCGGCACCTCGGCGGAAGCG
AACCTGAAGACCGTTAAGCTGGCCTCGGCAAAATACTATGATGCGCTACCGACCGAAGGCAATGAACACGGGCAGGCCTT
CCGCGATATTGAGCTGGAAAAAGAATTGTTGCTTGAGGCGCAAAATCTTGGCTTGGGCGCGCAGTTCGGCGGCAAATATT
TCGCTCACGATATTCGTGTCATCCGATTGCCGCGCCACGGGGCATCATGTCCGGTAGGTATGGGCGTGTCCTGCTCCGCG
GATCGAAACATTAAAGCGAAGATCAACCGGGAAGGGATCTGGATCGAGAAGCTGGAGCGCAATCCCGGTAAATATATTCC
TGAGGCGCTGCGCCAGGCGGGAGAGGGCGAGGCGGTGCGCGTTGATCTTAACCGTCCCATGAGCGAGATACTGCAACAAC
TGTCGCAGTATCCGGTATCAACGCGCCTGTCGCTGAACGGCACGATTATTGTGGGCCGCGACATCGCGCACGCTAAACTG
AAAGAACGGATGGACAGAGGCGAAGGTTTGCCGCAGTACATCAAAGATCATCCTATCTATTATGCGGGGCCGGCAAAAAC
GCCGGAAGGGTACGCCTCCGGGTCGCTTGGGCCGACTACCGCAGGACGGATGGACTCTTATGTTGATCAGCTCCAGTCGC
AGGGCGGCAGTATGATCATGCTGGCGAAAGGCAACCGCAGCCAGCAGGTGACCGATGCCTGTAAGAAACATGGCGGCTTC
TACCTGGGCAGTATCGGCGGCCCGGCAGCCGTTCTGGCGCAGGGCAGTATCAAGCGCCTGGAGTGCGTGGAATATCCTGA
ACTGGGTATGGAGGCTATCTGGAAAATTGAAGTGGAGGATTTTCCGGCCTTTATTCTGGTGGATGATAAAGGTAACGATT
TCTTCCAGCAGATTCAGTCATCGCAGTGCGCGCGCTGCGTTAAGTAA

Upstream 100 bases:

>100_bases
TTTTGTTAAAAAATTGTGTAGGATAGTGTTACTCGCTTTGAGTCGGGCAATGGAACACCTGCCCAGAGAATAACCATACC
GAGCGGTAAGTGAGAGCACA

Downstream 100 bases:

>100_bases
TGTCGCCGCCCTGCGGGGCGGTAACATGATTCGCTATTTTGCCGGAGAACGGCGCAAGCGCCTTATCCGGTCTACACCGT
TGTGTTGTTATCGTTAATAC

Product: fumarate hydratase class I, anaerobic

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSNKPFFYQDPFPLKKDDTEYYLLTSEHVSVAEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPQAS
ENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAALDMYKEVNT
GTNLPAQIDLYSVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVDKMRTLGTAACPPYHIAFVIGGTSAEA
NLKTVKLASAKYYDALPTEGNEHGQAFRDIELEKELLLEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINREGIWIEKLERNPGKYIPEALRQAGEGEAVRVDLNRPMSEILQQLSQYPVSTRLSLNGTIIVGRDIAHAKL
KERMDRGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQSQGGSMIMLAKGNRSQQVTDACKKHGGF
YLGSIGGPAAVLAQGSIKRLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQSSQCARCVK

Sequences:

>Translated_548_residues
MSNKPFFYQDPFPLKKDDTEYYLLTSEHVSVAEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPQAS
ENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAALDMYKEVNT
GTNLPAQIDLYSVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVDKMRTLGTAACPPYHIAFVIGGTSAEA
NLKTVKLASAKYYDALPTEGNEHGQAFRDIELEKELLLEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINREGIWIEKLERNPGKYIPEALRQAGEGEAVRVDLNRPMSEILQQLSQYPVSTRLSLNGTIIVGRDIAHAKL
KERMDRGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQSQGGSMIMLAKGNRSQQVTDACKKHGGF
YLGSIGGPAAVLAQGSIKRLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQSSQCARCVK
>Mature_547_residues
SNKPFFYQDPFPLKKDDTEYYLLTSEHVSVAEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPQASE
NDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAALDMYKEVNTG
TNLPAQIDLYSVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVDKMRTLGTAACPPYHIAFVIGGTSAEAN
LKTVKLASAKYYDALPTEGNEHGQAFRDIELEKELLLEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSAD
RNIKAKINREGIWIEKLERNPGKYIPEALRQAGEGEAVRVDLNRPMSEILQQLSQYPVSTRLSLNGTIIVGRDIAHAKLK
ERMDRGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQSQGGSMIMLAKGNRSQQVTDACKKHGGFY
LGSIGGPAAVLAQGSIKRLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQSSQCARCVK

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=548, Percent_Identity=95.8029197080292, Blast_Score=1096, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=548, Percent_Identity=89.051094890511, Blast_Score=1023, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=170, Percent_Identity=28.2352941176471, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60273; Mature: 60142

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKPFFYQDPFPLKKDDTEYYLLTSEHVSVAEFEGQEILKVAPEALTLLARQAFHDASF
CCCCCEEECCCCCCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCHH
MLRPAHQQQVADILRDPQASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCE
VWTGGGDEAALARGVYNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYSVDGDEYKF
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCEEE
LCIAKGGGSANKTYLYQETKALLTPGKLKNYLVDKMRTLGTAACPPYHIAFVIGGTSAEA
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLKTVKLASAKYYDALPTEGNEHGQAFRDIELEKELLLEAQNLGLGAQFGGKYFAHDIRV
CEEEEEEECCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLERNPGKYIPEALRQAGEGEAVR
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCCEEE
VDLNRPMSEILQQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDRGEGLPQYIKDHPIY
EECCCCHHHHHHHHHCCCCEEEEEECEEEEEECCHHHHHHHHHHHHCCCCHHHHHCCCEE
YAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQSQGGSMIMLAKGNRSQQVTDACKKHGGF
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQGSIKRLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQS
EEECCCCCCCEEECCCCCCEEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
SQCARCVK
HHHHHHCC
>Mature Secondary Structure 
SNKPFFYQDPFPLKKDDTEYYLLTSEHVSVAEFEGQEILKVAPEALTLLARQAFHDASF
CCCCEEECCCCCCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCHH
MLRPAHQQQVADILRDPQASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCE
VWTGGGDEAALARGVYNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYSVDGDEYKF
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCEEE
LCIAKGGGSANKTYLYQETKALLTPGKLKNYLVDKMRTLGTAACPPYHIAFVIGGTSAEA
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLKTVKLASAKYYDALPTEGNEHGQAFRDIELEKELLLEAQNLGLGAQFGGKYFAHDIRV
CEEEEEEECCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLERNPGKYIPEALRQAGEGEAVR
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCCEEE
VDLNRPMSEILQQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDRGEGLPQYIKDHPIY
EECCCCHHHHHHHHHCCCCEEEEEECEEEEEECCHHHHHHHHHHHHCCCCHHHHHCCCEE
YAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQSQGGSMIMLAKGNRSQQVTDACKKHGGF
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQGSIKRLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQS
EEECCCCCCCEEECCCCCCEEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
SQCARCVK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]