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The map label for this gene is prfH [H]

Identifier: 194735122

GI number: 194735122

Start: 370408

End: 371022

Strand: Direct

Name: prfH [H]

Synonym: SeSA_A0364

Alternate gene names: 194735122

Gene position: 370408-371022 (Clockwise)

Preceding gene: 194736029

Following gene: 194734973

Centisome position: 7.87

GC content: 54.96

Gene sequence:

>615_bases
ATGATCTTACTGCAACTTTCTTCCGCACAGGGACCGGACGAGTGTTGTCTGGCGGTAAAAAAAGCGCTGGATTGTCTGAC
GAAAGAAGCTGCCAGAGAAAAGGTATCGCTGACACGACTTGAAACGGAGCCGGGACGATTACCAGACACGCTGCGCTCCG
CACTGGTCTCGCTGGACGGTGAAAAAGCTATGGCTTTCAGTGAACGTTGGTGTGGAACGTTACTGTGGATTTGTACCAGC
CCGTATCGCCCGCACCATGGGCGAAAAAACTGGTACGTCGGCATTGGGCGCTTTTCCGCCGACGAACAGATGCAGTCTGA
TGAGATTCATTTCGAGACTCTGCGCTCTTCCGGCCCGGGTGGGCAGCACGTTAATAAAACCGACTCGGCAGTGCGGGCCA
CGCACCTGGCGAGCGGGATTAGCGTGAAGGTTCAGTCGGAGCGCAGTCAACATGCGAATAAGCGTCTGGCCCGGCTGCTG
ATTGCCTGGCGGCTGGAACAGCAACGGCAAAATGAGTGTGCGGCGTTGAAGTCTGAAAGGCGTCTGTTCCATCATCAGAT
TGAGCGCGGCAATCCGCTGCGGATATTTAAAGGAATGGCGTTTACGCCACAATAA

Upstream 100 bases:

>100_bases
TCGATAGCGTTGTGGATTGTCTGGCAGACGCCGGACTGATTACGCCGGTTGCCTGCCTGCGTCCGGTGCTGACGCTGAAA
ACCTCAGGGGAGAAAAGCGC

Downstream 100 bases:

>100_bases
TTAGGTGTTACCGGCGCTGTTATCGGGCTGTCTGGCTATTCGCGTGTAGCCGCAGCGCTATTTCAGCGCATCCAGGAGAG
CTTATCTGGCAATGGATACC

Product: peptide chain release factor-like protein

Products: NA

Alternate protein names: RF-H [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MILLQLSSAQGPDECCLAVKKALDCLTKEAAREKVSLTRLETEPGRLPDTLRSALVSLDGEKAMAFSERWCGTLLWICTS
PYRPHHGRKNWYVGIGRFSADEQMQSDEIHFETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
IAWRLEQQRQNECAALKSERRLFHHQIERGNPLRIFKGMAFTPQ

Sequences:

>Translated_204_residues
MILLQLSSAQGPDECCLAVKKALDCLTKEAAREKVSLTRLETEPGRLPDTLRSALVSLDGEKAMAFSERWCGTLLWICTS
PYRPHHGRKNWYVGIGRFSADEQMQSDEIHFETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
IAWRLEQQRQNECAALKSERRLFHHQIERGNPLRIFKGMAFTPQ
>Mature_204_residues
MILLQLSSAQGPDECCLAVKKALDCLTKEAAREKVSLTRLETEPGRLPDTLRSALVSLDGEKAMAFSERWCGTLLWICTS
PYRPHHGRKNWYVGIGRFSADEQMQSDEIHFETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
IAWRLEQQRQNECAALKSERRLFHHQIERGNPLRIFKGMAFTPQ

Specific function: Could be involved in translational termination [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=69, Percent_Identity=46.3768115942029, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787462, Length=58, Percent_Identity=55.1724137931034, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI2367172, Length=129, Percent_Identity=29.4573643410853, Blast_Score=63, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321295, Length=58, Percent_Identity=58.6206896551724, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921226, Length=70, Percent_Identity=51.4285714285714, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000352
- InterPro:   IPR017509 [H]

Pfam domain/function: PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 23032; Mature: 23032

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLQLSSAQGPDECCLAVKKALDCLTKEAAREKVSLTRLETEPGRLPDTLRSALVSLDG
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCC
EKAMAFSERWCGTLLWICTSPYRPHHGRKNWYVGIGRFSADEQMQSDEIHFETLRSSGPG
CHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCCCCHHHCCCCHHHHHHHCCCCC
GQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLLIAWRLEQQRQNECAALKSER
CCCCCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLFHHQIERGNPLRIFKGMAFTPQ
HHHHHHHCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MILLQLSSAQGPDECCLAVKKALDCLTKEAAREKVSLTRLETEPGRLPDTLRSALVSLDG
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCC
EKAMAFSERWCGTLLWICTSPYRPHHGRKNWYVGIGRFSADEQMQSDEIHFETLRSSGPG
CHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCCCCHHHCCCCHHHHHHHCCCCC
GQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLLIAWRLEQQRQNECAALKSER
CCCCCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLFHHQIERGNPLRIFKGMAFTPQ
HHHHHHHCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1695895; 9278503; 1408743 [H]