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The map label for this gene is nudF [H]

Identifier: 194734725

GI number: 194734725

Start: 3253970

End: 3254602

Strand: Reverse

Name: nudF [H]

Synonym: SeSA_A3371

Alternate gene names: 194734725

Gene position: 3254602-3253970 (Counterclockwise)

Preceding gene: 194734625

Following gene: 194733940

Centisome position: 69.11

GC content: 53.24

Gene sequence:

>633_bases
ATGCGTAAATCAGACAACTTGCCAGTTACTTTCACCAAAAGCGATGTAGAAATTATTGCACGGGAAACGCGCTATCGCGG
TTTTTTTTCATTGGATGTGTATCGATTCCGCCATCGTTTATTCAACGGAGAAATGAGTGGCGAGGTAAAGCGCGAAATTT
TTGAGCGCGGGCACGCCGCCGTCTTGCTACCCTTTGACCCTGAACGCGACGAGGTGGTGCTGGTCGAACAGATTCGTATT
GCCGCGTATGACACAAGCGTTACGCCGTGGCTGCTGGAGATGGTCGCCGGCATGATAGAAGAAGGCGAAACCATTGAGGC
GGTCGCCCGGCGAGAGGCGATGGAAGAAGCGGGGCTGACCGTAGGCCGCACCCGACCGGTCATCAGTTACCTGGCGAGTC
CAGGAGGAACCAGCGAGCGCTCATCTATTCTGGTGGGTGAAGTGGACGCCACGACCGCGGCAGGTATTCATGGTCTGGCG
GATGAAAACGAAGATATTCGGGTTCATGTGGTAAGCCGGGAACAGGCATACCAGTGGGTAGAAGAGGGGAAAATCGACAA
CGCAGCCGCAGTCATCGCTTTGCAATGGCTTCAGTTGCATCATCAAGAGTTAAAAAACGAGTGGAAAAAATGA

Upstream 100 bases:

>100_bases
CGAGCCTTAATTGGCACCAATTTACCACAAGGGTGATAAAACAGCAGTCAACCACCCTTGAAATTCATCATTTAATCATT
ATTGCTAGCAGGGCAGCACA

Downstream 100 bases:

>100_bases
AGCGTTATACACCTGACTTCCCAGAAATGATGCGTTTGTGCGAAACCAATTTTTCGCAATTGCGCCGCCTGCTGCCGCGC
AATGACGCCCCCGGTGAAAC

Product: ADP-ribose pyrophosphatase NudF

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MRKSDNLPVTFTKSDVEIIARETRYRGFFSLDVYRFRHRLFNGEMSGEVKREIFERGHAAVLLPFDPERDEVVLVEQIRI
AAYDTSVTPWLLEMVAGMIEEGETIEAVARREAMEEAGLTVGRTRPVISYLASPGGTSERSSILVGEVDATTAAGIHGLA
DENEDIRVHVVSREQAYQWVEEGKIDNAAAVIALQWLQLHHQELKNEWKK

Sequences:

>Translated_210_residues
MRKSDNLPVTFTKSDVEIIARETRYRGFFSLDVYRFRHRLFNGEMSGEVKREIFERGHAAVLLPFDPERDEVVLVEQIRI
AAYDTSVTPWLLEMVAGMIEEGETIEAVARREAMEEAGLTVGRTRPVISYLASPGGTSERSSILVGEVDATTAAGIHGLA
DENEDIRVHVVSREQAYQWVEEGKIDNAAAVIALQWLQLHHQELKNEWKK
>Mature_210_residues
MRKSDNLPVTFTKSDVEIIARETRYRGFFSLDVYRFRHRLFNGEMSGEVKREIFERGHAAVLLPFDPERDEVVLVEQIRI
AAYDTSVTPWLLEMVAGMIEEGETIEAVARREAMEEAGLTVGRTRPVISYLASPGGTSERSSILVGEVDATTAAGIHGLA
DENEDIRVHVVSREQAYQWVEEGKIDNAAAVIALQWLQLHHQELKNEWKK

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=208, Percent_Identity=87.9807692307692, Blast_Score=379, Evalue=1e-107,
Organism=Escherichia coli, GI1788810, Length=159, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 23781; Mature: 23781

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSDNLPVTFTKSDVEIIARETRYRGFFSLDVYRFRHRLFNGEMSGEVKREIFERGHAA
CCCCCCCCEEEECCHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHCCCEE
VLLPFDPERDEVVLVEQIRIAAYDTSVTPWLLEMVAGMIEEGETIEAVARREAMEEAGLT
EEEECCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
VGRTRPVISYLASPGGTSERSSILVGEVDATTAAGIHGLADENEDIRVHVVSREQAYQWV
CCCHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCEEEEEECHHHHHHHH
EEGKIDNAAAVIALQWLQLHHQELKNEWKK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKSDNLPVTFTKSDVEIIARETRYRGFFSLDVYRFRHRLFNGEMSGEVKREIFERGHAA
CCCCCCCCEEEECCHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHCCCEE
VLLPFDPERDEVVLVEQIRIAAYDTSVTPWLLEMVAGMIEEGETIEAVARREAMEEAGLT
EEEECCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
VGRTRPVISYLASPGGTSERSSILVGEVDATTAAGIHGLADENEDIRVHVVSREQAYQWV
CCCHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCEEEEEECHHHHHHHH
EEGKIDNAAAVIALQWLQLHHQELKNEWKK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]