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The map label for this gene is ogt [H]

Identifier: 194734500

GI number: 194734500

Start: 1726843

End: 1727358

Strand: Direct

Name: ogt [H]

Synonym: SeSA_A1784

Alternate gene names: 194734500

Gene position: 1726843-1727358 (Clockwise)

Preceding gene: 194735405

Following gene: 194734021

Centisome position: 36.67

GC content: 55.43

Gene sequence:

>516_bases
ATGCTGAGATTACTTGAAGAGAAGATAGCCACGCCATTAGGTCCGTTATGGGTGGTTTGCGATGAGCAGTTTCGACTGCG
GGCCATTGAGTGGGAACAGTACCGCGATCGTATGGAGCAACTGCTAAATATCCACTACCGTCACGAAGGCTATGAACGCC
TTTCTGCGACTAACCCCGGTGGACTCAGCGATAAGCTTGCAGATTATTTTGCAGGCAATCTCGCCGTAATTGATACCCTG
GAAACCGCCACGGGGGGCACACCTTTTCAACGGGAAGTATGGCAGGCGTTGCGCGCTATCCCCTGCGGGCAGGTGATGCA
CTATGGTCAACTGGCGGCGCAACTGGGACGACCGGGCGCCGCACGCGCAGTCGGTGCTGCGAATAGCGCGAACCCCATCA
GTATTGTTGTTCCCTGCCATCGCGTCATCGGGCGTAACGGCACTCTGACCGGATACGCCGGTGGCGTGCAGCGAAAAGAG
TGGCTATTACGCCATGAAGGCTATCTTTTATTATGA

Upstream 100 bases:

>100_bases
AATGTGTTATCCCTGGCTATCTTTTAAGGAGTATGGTTGCGGGTATTCCTGGCATGATATCTTGTCTCTTACGTTAGGCT
AAGACGATGTGAGAGAACGG

Downstream 100 bases:

>100_bases
ATATACAGGCAAAAAGTGCCTTATCGGTCACACTTTTATGTAAAGCAACAACAAATAAATTTCGTATTTTCAAATAGATA
AAAATATTCATCAAACTTAT

Product: O-6-alkylguanine-DNA:cysteine-protein methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MLRLLEEKIATPLGPLWVVCDEQFRLRAIEWEQYRDRMEQLLNIHYRHEGYERLSATNPGGLSDKLADYFAGNLAVIDTL
ETATGGTPFQREVWQALRAIPCGQVMHYGQLAAQLGRPGAARAVGAANSANPISIVVPCHRVIGRNGTLTGYAGGVQRKE
WLLRHEGYLLL

Sequences:

>Translated_171_residues
MLRLLEEKIATPLGPLWVVCDEQFRLRAIEWEQYRDRMEQLLNIHYRHEGYERLSATNPGGLSDKLADYFAGNLAVIDTL
ETATGGTPFQREVWQALRAIPCGQVMHYGQLAAQLGRPGAARAVGAANSANPISIVVPCHRVIGRNGTLTGYAGGVQRKE
WLLRHEGYLLL
>Mature_171_residues
MLRLLEEKIATPLGPLWVVCDEQFRLRAIEWEQYRDRMEQLLNIHYRHEGYERLSATNPGGLSDKLADYFAGNLAVIDTL
ETATGGTPFQREVWQALRAIPCGQVMHYGQLAAQLGRPGAARAVGAANSANPISIVVPCHRVIGRNGTLTGYAGGVQRKE
WLLRHEGYLLL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=83, Percent_Identity=50.6024096385542, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI1787596, Length=171, Percent_Identity=87.719298245614, Blast_Score=320, Evalue=4e-89,
Organism=Escherichia coli, GI1788542, Length=89, Percent_Identity=53.9325842696629, Blast_Score=103, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=104, Percent_Identity=37.5, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=104, Percent_Identity=37.5, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=46.9135802469136, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19052; Mature: 19052

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLLEEKIATPLGPLWVVCDEQFRLRAIEWEQYRDRMEQLLNIHYRHEGYERLSATNPG
CCCHHHHHHCCCCCCEEEEECCCHHEEEECHHHHHHHHHHHHHHHHHHCCHHHHCCCCCC
GLSDKLADYFAGNLAVIDTLETATGGTPFQREVWQALRAIPCGQVMHYGQLAAQLGRPGA
CCHHHHHHHHCCCEEEEEEHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
ARAVGAANSANPISIVVPCHRVIGRNGTLTGYAGGVQRKEWLLRHEGYLLL
HHHHCCCCCCCCEEEEEECHHHHCCCCCEECCCCCCHHHHHHHHCCCEEEC
>Mature Secondary Structure
MLRLLEEKIATPLGPLWVVCDEQFRLRAIEWEQYRDRMEQLLNIHYRHEGYERLSATNPG
CCCHHHHHHCCCCCCEEEEECCCHHEEEECHHHHHHHHHHHHHHHHHHCCHHHHCCCCCC
GLSDKLADYFAGNLAVIDTLETATGGTPFQREVWQALRAIPCGQVMHYGQLAAQLGRPGA
CCHHHHHHHHCCCEEEEEEHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
ARAVGAANSANPISIVVPCHRVIGRNGTLTGYAGGVQRKEWLLRHEGYLLL
HHHHCCCCCCCCEEEEEECHHHHCCCCCEECCCCCCHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]