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The map label for this gene is relA [H]

Identifier: 194334625

GI number: 194334625

Start: 1990210

End: 1992408

Strand: Reverse

Name: relA [H]

Synonym: Paes_1826

Alternate gene names: 194334625

Gene position: 1992408-1990210 (Counterclockwise)

Preceding gene: 194334626

Following gene: 194334623

Centisome position: 79.29

GC content: 49.52

Gene sequence:

>2199_bases
ATGTTAGCACAGATCGAAAAAGATCAATATGAAAAACTTCACGAGGTTTTAAGGCTTGCTCGAGCAAACCTTAAAAATTT
CGATGAATCTCTCGTTCAAAGAGCGTTCTTCATGTGTTACCGTGCGCATGAAGGTGAAAAACGTGCCTCGGGCGAGCCTT
TTTTCTATCATCCTGTGGAAGTCGCCAAGATCATGCTGACGGAACTTCCCCTTGACGGCGTCTCAGTCGCCGCAGCGCTG
CTGCATGATGTGATCGAAGATAGCGAATATACGTATGAGGACCTGGTTGCCGAACTCGGCTCTGAAGTTGCCGACATCGT
TGAAGGATTGACCAAGATTTCAGGGATCATGATCAATCGTGAGATCACCCAGGCAGAAGGGTTTCGCAAGATGTTGCTTT
CCATGGTCAAGGATATTCGGGTGATTCTCCTCAAATTCTGCGACAGGCTACATAACATGCGAACGCTTGGTGCGCTTCCT
GAACATCGACGTCTCAAGATCGCTCTTGAAACAAGGGATATCTATGCCCCTCTCGCACACCGCTTCGGTCTGGGTAAAAT
GAAGGTTGAATTGGAAAACCTTGCCTTCAAGTACATCGATCCTGAAATGTTCGAGATGATCCAGCAGAAGGTCCGGCTCA
GCAGGGGAGAGCGCATCGCCTATCTCGATAAAATGATCGTACCGATCAAGGATGATCTCCAGAAACAGGGCTTCAATCTT
GAAGTTCAAGGGCGCGCCAAGCATCTTTTTTCTATCTACAACAAGATGCGTTTGAAAAACAAGCGCTTTGAGGATATCCA
TGATCTTTACGGTATACGCATCGTTATCGATACAGAGCGTATATCTGACTGTTTTGCAGCCTACGGGTTTATTACTCAGA
AATATCCTCCTCTGCCTCAGCATTTCAAGGATTATATCTCCATTCCCAAGCACAATGGTTACCAGTCACTCCATTCGGCT
ATTCTCGGGCCCAGAGGGCACGTTGTCGAAGTGCAGATCCGCACGCGCAGAATGCATGAATTTGCCGAGTTTGGTGTCGC
TGCGCACTGGCGATACAAGGAGAAGGTTTCCAGAGATGATGCATCGGTCAATACCTTCCTCCGCTGGGCCCGGGAGTTGA
TCAACGATGCCGATTCAGCAGCATCCTTTATGGAGGGATTCAAGCTCAATCTGTACCATGACGAGATCTATCTCTTTACT
CCGAAGGGGGATATGAAAACCCTTCCTGCAGGTGCCACCCCGATCGATTTCGCCTATTCCATCCATACAGAGATCGGTAA
CGGCTGTATAGGAGCAAAGGTTAACGGCAAGATTGTCCGGCTCAATTCAGAACTTCGTTCAGGAGACCGGGTTGAAGTCA
TTACCTCGAGAAACCAGAAACCCAAAGCCGACTGGCTCAAATTCGTGGTGACCCATCGAGCCAAGCTCAAAATCCGTTCG
GCAATCAACGAAGAACGGCGTCTTCAGATTGAAAAAGGGCGCGGCATGTGGGAGAAAATGCTCTCAGGAACCCGTAAGCT
GCTCAACGATAACGACATTATCCGACAGGTCCGCAAGTACGGCATCAAAACCCCGGCTGACTTTTTCAGCGCTCTGGCCA
ACCACCAGATCGATCCGGAGAAGGTCATCAAGAGCTTTGCCGAAGAACACCGCCGTGCCGCTCTCGATGAAGGAGCTGAT
GCTGGAAGCCAATTCGATACCTTTGCCGAGCAGGCACGCAAAGTTCCTGAAGATGAGCCCGCCGGCAAGGGGCGTGACGA
GGTTACTATAGAGGGGCTTCCCAATATCGCGTATTCCTACGCTAAATGCTGCAAGCCGGTGCCTGGAGACGATATTATCG
GGTTTGTCACCAAAGAGGGGGTTGTCAAGATCCATCGCAAGAATTGCGTCAATGTCAGTAACGAGAGCCTTCTCAAAAGC
GAGCGGGTTGTCAGTGTTTCATGGAACCGTAAAGTTGAAACCGAATTTCTGGCAGGGATCAAGGTGATCGGCGAGGACCG
CATAGGCATAACCAATCAGATTACCGCGGTCATATCCAAATCTGATACCAATATCCGAAGCATTTCCCTCCACGCGCGCG
ACGGTATGTTTATCGGTACCGTTATGGTCTACGTGCGCAACATATCCCGCCTGACAGCCCTTATGGATAAAATCCGCAAG
ATCACCGGCGTTTTTTCCGTAGAACGACTCAGTAATTGA

Upstream 100 bases:

>100_bases
GTTGCGTGACGAGATTTCCAAAATCGAGCATGCTGCCGGCGGTAGCGCAAAACAACCCTGATAACCATTACTGAAAACGT
TTCCTGAGGTAGTAGCCGAT

Downstream 100 bases:

>100_bases
AGAGCGTTTGAAAGGGGGGACGTTCTTTCTTTGCGATACTTGGGCGTGCTTTGCGTGGGTGAACGGAGGTGGGAGCGCCT
GCGGCGCGGGGTGCCCTTCG

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 732; Mature: 732

Protein sequence:

>732_residues
MLAQIEKDQYEKLHEVLRLARANLKNFDESLVQRAFFMCYRAHEGEKRASGEPFFYHPVEVAKIMLTELPLDGVSVAAAL
LHDVIEDSEYTYEDLVAELGSEVADIVEGLTKISGIMINREITQAEGFRKMLLSMVKDIRVILLKFCDRLHNMRTLGALP
EHRRLKIALETRDIYAPLAHRFGLGKMKVELENLAFKYIDPEMFEMIQQKVRLSRGERIAYLDKMIVPIKDDLQKQGFNL
EVQGRAKHLFSIYNKMRLKNKRFEDIHDLYGIRIVIDTERISDCFAAYGFITQKYPPLPQHFKDYISIPKHNGYQSLHSA
ILGPRGHVVEVQIRTRRMHEFAEFGVAAHWRYKEKVSRDDASVNTFLRWARELINDADSAASFMEGFKLNLYHDEIYLFT
PKGDMKTLPAGATPIDFAYSIHTEIGNGCIGAKVNGKIVRLNSELRSGDRVEVITSRNQKPKADWLKFVVTHRAKLKIRS
AINEERRLQIEKGRGMWEKMLSGTRKLLNDNDIIRQVRKYGIKTPADFFSALANHQIDPEKVIKSFAEEHRRAALDEGAD
AGSQFDTFAEQARKVPEDEPAGKGRDEVTIEGLPNIAYSYAKCCKPVPGDDIIGFVTKEGVVKIHRKNCVNVSNESLLKS
ERVVSVSWNRKVETEFLAGIKVIGEDRIGITNQITAVISKSDTNIRSISLHARDGMFIGTVMVYVRNISRLTALMDKIRK
ITGVFSVERLSN

Sequences:

>Translated_732_residues
MLAQIEKDQYEKLHEVLRLARANLKNFDESLVQRAFFMCYRAHEGEKRASGEPFFYHPVEVAKIMLTELPLDGVSVAAAL
LHDVIEDSEYTYEDLVAELGSEVADIVEGLTKISGIMINREITQAEGFRKMLLSMVKDIRVILLKFCDRLHNMRTLGALP
EHRRLKIALETRDIYAPLAHRFGLGKMKVELENLAFKYIDPEMFEMIQQKVRLSRGERIAYLDKMIVPIKDDLQKQGFNL
EVQGRAKHLFSIYNKMRLKNKRFEDIHDLYGIRIVIDTERISDCFAAYGFITQKYPPLPQHFKDYISIPKHNGYQSLHSA
ILGPRGHVVEVQIRTRRMHEFAEFGVAAHWRYKEKVSRDDASVNTFLRWARELINDADSAASFMEGFKLNLYHDEIYLFT
PKGDMKTLPAGATPIDFAYSIHTEIGNGCIGAKVNGKIVRLNSELRSGDRVEVITSRNQKPKADWLKFVVTHRAKLKIRS
AINEERRLQIEKGRGMWEKMLSGTRKLLNDNDIIRQVRKYGIKTPADFFSALANHQIDPEKVIKSFAEEHRRAALDEGAD
AGSQFDTFAEQARKVPEDEPAGKGRDEVTIEGLPNIAYSYAKCCKPVPGDDIIGFVTKEGVVKIHRKNCVNVSNESLLKS
ERVVSVSWNRKVETEFLAGIKVIGEDRIGITNQITAVISKSDTNIRSISLHARDGMFIGTVMVYVRNISRLTALMDKIRK
ITGVFSVERLSN
>Mature_732_residues
MLAQIEKDQYEKLHEVLRLARANLKNFDESLVQRAFFMCYRAHEGEKRASGEPFFYHPVEVAKIMLTELPLDGVSVAAAL
LHDVIEDSEYTYEDLVAELGSEVADIVEGLTKISGIMINREITQAEGFRKMLLSMVKDIRVILLKFCDRLHNMRTLGALP
EHRRLKIALETRDIYAPLAHRFGLGKMKVELENLAFKYIDPEMFEMIQQKVRLSRGERIAYLDKMIVPIKDDLQKQGFNL
EVQGRAKHLFSIYNKMRLKNKRFEDIHDLYGIRIVIDTERISDCFAAYGFITQKYPPLPQHFKDYISIPKHNGYQSLHSA
ILGPRGHVVEVQIRTRRMHEFAEFGVAAHWRYKEKVSRDDASVNTFLRWARELINDADSAASFMEGFKLNLYHDEIYLFT
PKGDMKTLPAGATPIDFAYSIHTEIGNGCIGAKVNGKIVRLNSELRSGDRVEVITSRNQKPKADWLKFVVTHRAKLKIRS
AINEERRLQIEKGRGMWEKMLSGTRKLLNDNDIIRQVRKYGIKTPADFFSALANHQIDPEKVIKSFAEEHRRAALDEGAD
AGSQFDTFAEQARKVPEDEPAGKGRDEVTIEGLPNIAYSYAKCCKPVPGDDIIGFVTKEGVVKIHRKNCVNVSNESLLKS
ERVVSVSWNRKVETEFLAGIKVIGEDRIGITNQITAVISKSDTNIRSISLHARDGMFIGTVMVYVRNISRLTALMDKIRK
ITGVFSVERLSN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=709, Percent_Identity=38.787023977433, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1789147, Length=721, Percent_Identity=31.3453536754508, Blast_Score=398, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 83564; Mature: 83564

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAQIEKDQYEKLHEVLRLARANLKNFDESLVQRAFFMCYRAHEGEKRASGEPFFYHPVE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHH
VAKIMLTELPLDGVSVAAALLHDVIEDSEYTYEDLVAELGSEVADIVEGLTKISGIMINR
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
EITQAEGFRKMLLSMVKDIRVILLKFCDRLHNMRTLGALPEHRRLKIALETRDIYAPLAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHH
RFGLGKMKVELENLAFKYIDPEMFEMIQQKVRLSRGERIAYLDKMIVPIKDDLQKQGFNL
HCCCCCEEEEHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEHHHHHHCCCHHHHHHCCCCE
EVQGRAKHLFSIYNKMRLKNKRFEDIHDLYGIRIVIDTERISDCFAAYGFITQKYPPLPQ
EECHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHCCCCCCHH
HFKDYISIPKHNGYQSLHSAILGPRGHVVEVQIRTRRMHEFAEFGVAAHWRYKEKVSRDD
HHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCHHEEHHHHHHCCCC
ASVNTFLRWARELINDADSAASFMEGFKLNLYHDEIYLFTPKGDMKTLPAGATPIDFAYS
HHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECEEEEECCCCCCCCCCCCCCCCHHHEE
IHTEIGNGCIGAKVNGKIVRLNSELRSGDRVEVITSRNQKPKADWLKFVVTHRAKLKIRS
EEHHHCCCEECCEECCEEEEEEHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
AINEERRLQIEKGRGMWEKMLSGTRKLLNDNDIIRQVRKYGIKTPADFFSALANHQIDPE
HCCHHHCEEEHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHH
KVIKSFAEEHRRAALDEGADAGSQFDTFAEQARKVPEDEPAGKGRDEVTIEGLPNIAYSY
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHH
AKCCKPVPGDDIIGFVTKEGVVKIHRKNCVNVSNESLLKSERVVSVSWNRKVETEFLAGI
HHHCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHCCEEEEECCCCHHHHHHHHH
KVIGEDRIGITNQITAVISKSDTNIRSISLHARDGMFIGTVMVYVRNISRLTALMDKIRK
HEECCCCCCCHHHHHHEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ITGVFSVERLSN
HHHHHHHHHHCC
>Mature Secondary Structure
MLAQIEKDQYEKLHEVLRLARANLKNFDESLVQRAFFMCYRAHEGEKRASGEPFFYHPVE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHH
VAKIMLTELPLDGVSVAAALLHDVIEDSEYTYEDLVAELGSEVADIVEGLTKISGIMINR
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
EITQAEGFRKMLLSMVKDIRVILLKFCDRLHNMRTLGALPEHRRLKIALETRDIYAPLAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHH
RFGLGKMKVELENLAFKYIDPEMFEMIQQKVRLSRGERIAYLDKMIVPIKDDLQKQGFNL
HCCCCCEEEEHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEHHHHHHCCCHHHHHHCCCCE
EVQGRAKHLFSIYNKMRLKNKRFEDIHDLYGIRIVIDTERISDCFAAYGFITQKYPPLPQ
EECHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHCCCCCCHH
HFKDYISIPKHNGYQSLHSAILGPRGHVVEVQIRTRRMHEFAEFGVAAHWRYKEKVSRDD
HHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCHHEEHHHHHHCCCC
ASVNTFLRWARELINDADSAASFMEGFKLNLYHDEIYLFTPKGDMKTLPAGATPIDFAYS
HHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECEEEEECCCCCCCCCCCCCCCCHHHEE
IHTEIGNGCIGAKVNGKIVRLNSELRSGDRVEVITSRNQKPKADWLKFVVTHRAKLKIRS
EEHHHCCCEECCEECCEEEEEEHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
AINEERRLQIEKGRGMWEKMLSGTRKLLNDNDIIRQVRKYGIKTPADFFSALANHQIDPE
HCCHHHCEEEHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHH
KVIKSFAEEHRRAALDEGADAGSQFDTFAEQARKVPEDEPAGKGRDEVTIEGLPNIAYSY
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHH
AKCCKPVPGDDIIGFVTKEGVVKIHRKNCVNVSNESLLKSERVVSVSWNRKVETEFLAGI
HHHCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHCCEEEEECCCCHHHHHHHHH
KVIGEDRIGITNQITAVISKSDTNIRSISLHARDGMFIGTVMVYVRNISRLTALMDKIRK
HEECCCCCCCHHHHHHEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ITGVFSVERLSN
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9383190; 9384377 [H]