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The map label for this gene is cysD [H]

Identifier: 194334559

GI number: 194334559

Start: 1918388

End: 1919296

Strand: Reverse

Name: cysD [H]

Synonym: Paes_1757

Alternate gene names: 194334559

Gene position: 1919296-1918388 (Counterclockwise)

Preceding gene: 194334560

Following gene: 194334558

Centisome position: 76.38

GC content: 47.63

Gene sequence:

>909_bases
ATGATTTCATATAAAATATCGCATCTCAAACAACTTGAGGCTGAGTCCATCCATATCATCCGAGAAGTGGCTGCAGAGTT
CGAGAACCCTGTGATGCTTTATTCTATAGGTAAAGACTCTTCAGTCATGGTACGCCTTGCAGAAAAGGCTTTTTATCCGG
GGAAGGTTCCATTCCCCCTTTTGCATATTGATTCCAAGTGGAAGTTTCGCGAGATGATCAATTTTCGTGATGCCTACGCT
AAAGAGAATAATTGGGACCTGATTGTGCACTCCAACATGGAGGCATTTGAACAGGGAGTCGGACCATTTTCCCATGGCTC
CCAGGTGCATACTGACCTGATGAAAACCCAGGCATTGCTGCAGGCGTTGAACAAGTACAAGTTCGATGCTGCGTTTGGTG
GCGCACGGCGCGATGAGGAGAAGTCGCGAGCTAAGGAGAGGATATTCTCGTTCCGTGACCGTTTTAACCAGTGGGATCCG
AAAAACCAGCGGCCTGAGCTGTGGGATATCTACAATACCAAGGTTCATAAAGGGGAGTCTATTCGGGTCTTTCCGATATC
CAACTGGACTGAGCTGGATATCTGGGAGTATATCAGGTTGGAAGAGATTCCCATTGTTCCGTTGTACTATGCTAAGCAGC
GCCCTATTGTCAACCTTGATGGCAGCCTCATCATGGTTGATGATGAGCGTATGCCTGAAGAGATGAGAGAAAAGGCTGAG
ATGAAGATGGTCCGGTTCAGGACGCTTGGGTGTTACCCGCTGACCGGTGCAGTGGAGTCCTCTGCTTCTACCATTGAAGA
GATCGTGCAGGAGATGATGATCACAACAAAGTCTGAACGTACAACACGCGTGATTGACTTTGACCAGGAAGCTTCAATGG
AACAGAAAAAGAGGGAGGGCTACTTTTAA

Upstream 100 bases:

>100_bases
AGTGGCTTGAACGTGTCACTTCGAAAGCTTATCGGAGGTATTACAAGGAAACGTATGGTATTCATTAAACTGGGTATTTT
TAATAAATCAAATAAGTTCT

Downstream 100 bases:

>100_bases
TGCAGGATGTGATGAGTTCTACAGATATTGCACAGTTTCTTGAACAGGACCAGAACAAGGATCTGCTTCGTTTTCTGACT
GCCGGATCAGTTGATGACGG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MISYKISHLKQLEAESIHIIREVAAEFENPVMLYSIGKDSSVMVRLAEKAFYPGKVPFPLLHIDSKWKFREMINFRDAYA
KENNWDLIVHSNMEAFEQGVGPFSHGSQVHTDLMKTQALLQALNKYKFDAAFGGARRDEEKSRAKERIFSFRDRFNQWDP
KNQRPELWDIYNTKVHKGESIRVFPISNWTELDIWEYIRLEEIPIVPLYYAKQRPIVNLDGSLIMVDDERMPEEMREKAE
MKMVRFRTLGCYPLTGAVESSASTIEEIVQEMMITTKSERTTRVIDFDQEASMEQKKREGYF

Sequences:

>Translated_302_residues
MISYKISHLKQLEAESIHIIREVAAEFENPVMLYSIGKDSSVMVRLAEKAFYPGKVPFPLLHIDSKWKFREMINFRDAYA
KENNWDLIVHSNMEAFEQGVGPFSHGSQVHTDLMKTQALLQALNKYKFDAAFGGARRDEEKSRAKERIFSFRDRFNQWDP
KNQRPELWDIYNTKVHKGESIRVFPISNWTELDIWEYIRLEEIPIVPLYYAKQRPIVNLDGSLIMVDDERMPEEMREKAE
MKMVRFRTLGCYPLTGAVESSASTIEEIVQEMMITTKSERTTRVIDFDQEASMEQKKREGYF
>Mature_302_residues
MISYKISHLKQLEAESIHIIREVAAEFENPVMLYSIGKDSSVMVRLAEKAFYPGKVPFPLLHIDSKWKFREMINFRDAYA
KENNWDLIVHSNMEAFEQGVGPFSHGSQVHTDLMKTQALLQALNKYKFDAAFGGARRDEEKSRAKERIFSFRDRFNQWDP
KNQRPELWDIYNTKVHKGESIRVFPISNWTELDIWEYIRLEEIPIVPLYYAKQRPIVNLDGSLIMVDDERMPEEMREKAE
MKMVRFRTLGCYPLTGAVESSASTIEEIVQEMMITTKSERTTRVIDFDQEASMEQKKREGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=302, Percent_Identity=67.8807947019868, Blast_Score=436, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 35512; Mature: 35512

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISYKISHLKQLEAESIHIIREVAAEFENPVMLYSIGKDSSVMVRLAEKAFYPGKVPFPL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEE
LHIDSKWKFREMINFRDAYAKENNWDLIVHSNMEAFEQGVGPFSHGSQVHTDLMKTQALL
EEECCCHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCCCCCCHHHHHHHHHHHHH
QALNKYKFDAAFGGARRDEEKSRAKERIFSFRDRFNQWDPKNQRPELWDIYNTKVHKGES
HHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCEECCCCE
IRVFPISNWTELDIWEYIRLEEIPIVPLYYAKQRPIVNLDGSLIMVDDERMPEEMREKAE
EEEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCCEEECCCCEEEEECCCCCHHHHHHHH
MKMVRFRTLGCYPLTGAVESSASTIEEIVQEMMITTKSERTTRVIDFDQEASMEQKKREG
HHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCC
YF
CC
>Mature Secondary Structure
MISYKISHLKQLEAESIHIIREVAAEFENPVMLYSIGKDSSVMVRLAEKAFYPGKVPFPL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEE
LHIDSKWKFREMINFRDAYAKENNWDLIVHSNMEAFEQGVGPFSHGSQVHTDLMKTQALL
EEECCCHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCCCCCCHHHHHHHHHHHHH
QALNKYKFDAAFGGARRDEEKSRAKERIFSFRDRFNQWDPKNQRPELWDIYNTKVHKGES
HHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCEECCCCE
IRVFPISNWTELDIWEYIRLEEIPIVPLYYAKQRPIVNLDGSLIMVDDERMPEEMREKAE
EEEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCCEEECCCCEEEEECCCCCHHHHHHHH
MKMVRFRTLGCYPLTGAVESSASTIEEIVQEMMITTKSERTTRVIDFDQEASMEQKKREG
HHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCC
YF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA