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The map label for this gene is ilvI [H]

Identifier: 194333577

GI number: 194333577

Start: 793172

End: 794893

Strand: Reverse

Name: ilvI [H]

Synonym: Paes_0743

Alternate gene names: 194333577

Gene position: 794893-793172 (Counterclockwise)

Preceding gene: 194333578

Following gene: 194333576

Centisome position: 31.63

GC content: 50.0

Gene sequence:

>1722_bases
ATGGTTACATCAGGCCAAACAATGAACGGCTCTGAAATATTGTTCGAATGTCTCCGACGCGAACATGTTGAGTATATTTT
CGGCTACCCCGGCGGAGCCCTGCTGCAGGTTTATGAAACCCTCTATGATGTCAAGGACATCAAGCACATTCTTGTTCGTC
ACGAACAGGGCGCTGCCCATATGGCTGAAGGCTATGCGCGTGCAACCGGAAAACCCGGCATCGTTCTGGTTACCTCAGGT
CCCGGAGCCACCAATACGGTTACCGGCATTACCAATGCTTACATGGATTCATCGCCGATGGTTGTTTTCACCGGCCAGGT
ACCGAGCAATCTCATCGGCAACGACGCTTTTCAGGAAGCCGATATTGTCGGCATTACGCGGCCTATAACAAAACATAACT
TCCTGGTCAAGGATGTCAAAGATCTGGCTCTGACCATCAGAAAAGCATTTTATCTGGCAACACATGGCAGACCAGGCCCG
GTTCTCGTTGACCTGCCTAAGGATGTCATGAATTCCGAATGCATCTTCGACTGGCCAAAAACAATTGACATAAGAGGGTT
CAAGCCGACCATAAAGACCCATGAAAATCAGGTCAAAAAAGCTGCAAAAATGATTGCAAAAGCAAAAAAACCGTTGCTCT
ATGTCGGTGGGGGCGTCATTGCTGCCGAGGCATCGGAGGAACTGAGAAAGCTTGCAATCGCACAGAACATTCCGGTCACC
ATGACGCTGCAGGGTCTGGGCGCGTTTCCCGGCAACGACCCGCTCAGCATGGGTATGCTCGGTATGCATGGCACCTACTG
GGCAAACCAGGCCGTCAACAACTGCGATCTCCTGATCTCCGTCGGAGCTCGTTTTGACGACAGGGTAACGGGCAAAACCG
AAACCTTTGCAACGCACGCATATAAAATTCATAACGACATCGATCCGACAAATATCGATAAGAACATAAAAGTCGATCTT
CCTGTTGTCGGAGACGCAAAACAGTTCCTTCAGGGAATACTGGATGCACTTCCCAAAACCAAAGAAAACCGAAAGCCCTG
GCTGGCAGAGATCGAAAAGTGGCGCTCGCAATGTCCGCTCACTTACACGATCGACGATGACTCGCTGAAAACCGAATTTG
TCATAGAGGAGGTTTCACGACAGACACACGGTGATGCGGTTATCACCACCGATGTCGGCCAGCACCAGATGTGGACGAGC
CAGTACTATTCATTCAGAAACCCGAGATCTATACTGACCAGCGGCGGCCTGGGAACAATGGGCTACGGGCTTCCGGCAGC
AATAGGAGCTGCTTTCGGCGTTCACGACAGGCCTGTAGTGCTCTTTTGCGGTGATGGAGGGTTCATGATGAACATTCAGG
AACTTGTCACCGCGGTATACTACAAGCTTCCGATAAAAATATTCCTGATCAACAACAATTTTCTCGGCATGGTCCGTCAG
TGGCAGGAGCTGTTTCATCAGGAAAAATATTCATTTACCGACCTTGAGGACAGCAATCCCGATTTCATCCGGGTTGTCGA
AGCATTTGGATGCAAGGCGCGCAGAGCGGAAAATCCTGAGGAGGCCCGCCAGGCTATCAGCGAGGCACTTGCCTACAATG
ACGGTCCGTGCTTTGTTGAATTCCGGGTCGTCAAAAAAGATATGGTTTTTCCGATGGTTCCTGCAGGCGCATCAATTTCA
GATATGCTTCTTGACAGGCTGAATCCTAAAACAATGGTTTAA

Upstream 100 bases:

>100_bases
TTAAAAAAGGATATCTGGCCCGCTACGCGCACATGGTGACGTCAGCAAATACAGGGGCGATACTCAAAAATCCAGCTATT
AATCTTTAAATATCCGATCC

Downstream 100 bases:

>100_bases
GAGAGCTGCTATGAAACATATTATCTCCGTACTGGTTGAAAACAAGTTCGGCACACTCAACAGGGTCGCCGCCATGTTCA
GCGCACGCGGCTTCAATCTC

Product: acetolactate synthase large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MVTSGQTMNGSEILFECLRREHVEYIFGYPGGALLQVYETLYDVKDIKHILVRHEQGAAHMAEGYARATGKPGIVLVTSG
PGATNTVTGITNAYMDSSPMVVFTGQVPSNLIGNDAFQEADIVGITRPITKHNFLVKDVKDLALTIRKAFYLATHGRPGP
VLVDLPKDVMNSECIFDWPKTIDIRGFKPTIKTHENQVKKAAKMIAKAKKPLLYVGGGVIAAEASEELRKLAIAQNIPVT
MTLQGLGAFPGNDPLSMGMLGMHGTYWANQAVNNCDLLISVGARFDDRVTGKTETFATHAYKIHNDIDPTNIDKNIKVDL
PVVGDAKQFLQGILDALPKTKENRKPWLAEIEKWRSQCPLTYTIDDDSLKTEFVIEEVSRQTHGDAVITTDVGQHQMWTS
QYYSFRNPRSILTSGGLGTMGYGLPAAIGAAFGVHDRPVVLFCGDGGFMMNIQELVTAVYYKLPIKIFLINNNFLGMVRQ
WQELFHQEKYSFTDLEDSNPDFIRVVEAFGCKARRAENPEEARQAISEALAYNDGPCFVEFRVVKKDMVFPMVPAGASIS
DMLLDRLNPKTMV

Sequences:

>Translated_573_residues
MVTSGQTMNGSEILFECLRREHVEYIFGYPGGALLQVYETLYDVKDIKHILVRHEQGAAHMAEGYARATGKPGIVLVTSG
PGATNTVTGITNAYMDSSPMVVFTGQVPSNLIGNDAFQEADIVGITRPITKHNFLVKDVKDLALTIRKAFYLATHGRPGP
VLVDLPKDVMNSECIFDWPKTIDIRGFKPTIKTHENQVKKAAKMIAKAKKPLLYVGGGVIAAEASEELRKLAIAQNIPVT
MTLQGLGAFPGNDPLSMGMLGMHGTYWANQAVNNCDLLISVGARFDDRVTGKTETFATHAYKIHNDIDPTNIDKNIKVDL
PVVGDAKQFLQGILDALPKTKENRKPWLAEIEKWRSQCPLTYTIDDDSLKTEFVIEEVSRQTHGDAVITTDVGQHQMWTS
QYYSFRNPRSILTSGGLGTMGYGLPAAIGAAFGVHDRPVVLFCGDGGFMMNIQELVTAVYYKLPIKIFLINNNFLGMVRQ
WQELFHQEKYSFTDLEDSNPDFIRVVEAFGCKARRAENPEEARQAISEALAYNDGPCFVEFRVVKKDMVFPMVPAGASIS
DMLLDRLNPKTMV
>Mature_573_residues
MVTSGQTMNGSEILFECLRREHVEYIFGYPGGALLQVYETLYDVKDIKHILVRHEQGAAHMAEGYARATGKPGIVLVTSG
PGATNTVTGITNAYMDSSPMVVFTGQVPSNLIGNDAFQEADIVGITRPITKHNFLVKDVKDLALTIRKAFYLATHGRPGP
VLVDLPKDVMNSECIFDWPKTIDIRGFKPTIKTHENQVKKAAKMIAKAKKPLLYVGGGVIAAEASEELRKLAIAQNIPVT
MTLQGLGAFPGNDPLSMGMLGMHGTYWANQAVNNCDLLISVGARFDDRVTGKTETFATHAYKIHNDIDPTNIDKNIKVDL
PVVGDAKQFLQGILDALPKTKENRKPWLAEIEKWRSQCPLTYTIDDDSLKTEFVIEEVSRQTHGDAVITTDVGQHQMWTS
QYYSFRNPRSILTSGGLGTMGYGLPAAIGAAFGVHDRPVVLFCGDGGFMMNIQELVTAVYYKLPIKIFLINNNFLGMVRQ
WQELFHQEKYSFTDLEDSNPDFIRVVEAFGCKARRAENPEEARQAISEALAYNDGPCFVEFRVVKKDMVFPMVPAGASIS
DMLLDRLNPKTMV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=567, Percent_Identity=28.2186948853616, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI21361361, Length=521, Percent_Identity=24.9520153550864, Blast_Score=139, Evalue=7e-33,
Organism=Escherichia coli, GI87081685, Length=570, Percent_Identity=46.140350877193, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1790104, Length=568, Percent_Identity=41.9014084507042, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1786717, Length=539, Percent_Identity=32.6530612244898, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1787096, Length=547, Percent_Identity=27.9707495429616, Blast_Score=188, Evalue=1e-48,
Organism=Escherichia coli, GI1788716, Length=485, Percent_Identity=26.8041237113402, Blast_Score=158, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17531299, Length=561, Percent_Identity=26.0249554367201, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=561, Percent_Identity=26.0249554367201, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=578, Percent_Identity=24.7404844290657, Blast_Score=132, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=585, Percent_Identity=45.982905982906, Blast_Score=490, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6320123, Length=582, Percent_Identity=23.5395189003436, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=516, Percent_Identity=23.6434108527132, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320816, Length=500, Percent_Identity=24.4, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323073, Length=516, Percent_Identity=23.2558139534884, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=543, Percent_Identity=24.1252302025783, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922626, Length=563, Percent_Identity=25.3996447602131, Blast_Score=166, Evalue=5e-41,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63435; Mature: 63435

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTSGQTMNGSEILFECLRREHVEYIFGYPGGALLQVYETLYDVKDIKHILVRHEQGAAH
CCCCCCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MAEGYARATGKPGIVLVTSGPGATNTVTGITNAYMDSSPMVVFTGQVPSNLIGNDAFQEA
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCEEEEECCCCCCHHCCCCCCCC
DIVGITRPITKHNFLVKDVKDLALTIRKAFYLATHGRPGPVLVDLPKDVMNSECIFDWPK
CEEEEECCCHHCCHHHHHHHHHHHHHHHHHHEEECCCCCCEEEECCHHHCCCCEEEECCC
TIDIRGFKPTIKTHENQVKKAAKMIAKAKKPLLYVGGGVIAAEASEELRKLAIAQNIPVT
EEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECHHHHHHHHHHHCCCCEE
MTLQGLGAFPGNDPLSMGMLGMHGTYWANQAVNNCDLLISVGARFDDRVTGKTETFATHA
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHEEEE
YKIHNDIDPTNIDKNIKVDLPVVGDAKQFLQGILDALPKTKENRKPWLAEIEKWRSQCPL
EEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCE
TYTIDDDSLKTEFVIEEVSRQTHGDAVITTDVGQHQMWTSQYYSFRNPRSILTSGGLGTM
EEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHCCCCCCC
GYGLPAAIGAAFGVHDRPVVLFCGDGGFMMNIQELVTAVYYKLPIKIFLINNNFLGMVRQ
CCCHHHHHHHHCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHCCEEEEEECCCHHHHHHH
WQELFHQEKYSFTDLEDSNPDFIRVVEAFGCKARRAENPEEARQAISEALAYNDGPCFVE
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCCCCEEEE
FRVVKKDMVFPMVPAGASISDMLLDRLNPKTMV
EEEECCCCCCEECCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVTSGQTMNGSEILFECLRREHVEYIFGYPGGALLQVYETLYDVKDIKHILVRHEQGAAH
CCCCCCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MAEGYARATGKPGIVLVTSGPGATNTVTGITNAYMDSSPMVVFTGQVPSNLIGNDAFQEA
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCEEEEECCCCCCHHCCCCCCCC
DIVGITRPITKHNFLVKDVKDLALTIRKAFYLATHGRPGPVLVDLPKDVMNSECIFDWPK
CEEEEECCCHHCCHHHHHHHHHHHHHHHHHHEEECCCCCCEEEECCHHHCCCCEEEECCC
TIDIRGFKPTIKTHENQVKKAAKMIAKAKKPLLYVGGGVIAAEASEELRKLAIAQNIPVT
EEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECHHHHHHHHHHHCCCCEE
MTLQGLGAFPGNDPLSMGMLGMHGTYWANQAVNNCDLLISVGARFDDRVTGKTETFATHA
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHEEEE
YKIHNDIDPTNIDKNIKVDLPVVGDAKQFLQGILDALPKTKENRKPWLAEIEKWRSQCPL
EEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCE
TYTIDDDSLKTEFVIEEVSRQTHGDAVITTDVGQHQMWTSQYYSFRNPRSILTSGGLGTM
EEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHCCCCCCC
GYGLPAAIGAAFGVHDRPVVLFCGDGGFMMNIQELVTAVYYKLPIKIFLINNNFLGMVRQ
CCCHHHHHHHHCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHCCEEEEEECCCHHHHHHH
WQELFHQEKYSFTDLEDSNPDFIRVVEAFGCKARRAENPEEARQAISEALAYNDGPCFVE
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCCCCEEEE
FRVVKKDMVFPMVPAGASISDMLLDRLNPKTMV
EEEECCCCCCEECCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10555290 [H]