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The map label for this gene is tyrS [H]

Identifier: 194333540

GI number: 194333540

Start: 752015

End: 753235

Strand: Reverse

Name: tyrS [H]

Synonym: Paes_0706

Alternate gene names: 194333540

Gene position: 753235-752015 (Counterclockwise)

Preceding gene: 194333541

Following gene: 194333539

Centisome position: 29.97

GC content: 47.5

Gene sequence:

>1221_bases
ATGACTTTTCCACCGATCAAAGAACAACTCGATCTCATTATCAATAACACCGTCGAGGTCATCAGTGAAGAAGAGCTCGA
AAAAAAACTCGACAAGAGCATAAAAACCGGAAAACCACTGAAGGTTAAACTCGGGGCAGATCCCTCGCGTCCCGATCTCC
ATCTCGGCCACTCGGTCGTACTGCGCAAGCTCCGCGATTTTCAGGACCTTGGCCATGAAGCAATCCTCATCATCGGAGAC
TTTACCGCCATGATCGGCGATCCGTCGGGAAAAAGCAAAACCCGTCCACAGTTATCTGCTCAAGAAGCCAGAGAGAACGG
AGAAACCTATTTTGAGCAGGCATCGAAAATCCTTGACCCGGAAAAAACAACCATCTGTTACAATTCGGAGTGGCTCGGAA
AAATGACCTTTTCCGACGTCATAAGGCTCTCAAGCCACTATACGGTGGCAAGAATGCTTGAAAGAGATGACTTTGAGCGC
CGCTACCAGTCCTGTGAGCCCATTTCAATACATGAATTTCTCTACCCGCTGGCTCAGGGAATGGACTCCGTCCACCTGAA
AAACGACATCGAACTTGGAGGGACAGACCAGAAATTCAACCTTCTGGTCGGAAGAGATCTCCAGAGAGAGTACCATATCG
ATCCGCAGGTCTGCATCACCATGCCTCTTCTCGTCGGGACCTTCGGTGACGAAAAAATGTCAAAATCCCTTGGCAACGCA
ATCTCCTTTACCGACAGCGCCCAGGACGTCTACGGCAAAACCCTCTCCATCCCGGACAGCCTCATTGAAACATGGTTCAA
GCTGCTTGTCCCTCAACCGAGACAGTCAATAAAACTGTTTATGGAAATCATCCGTCAGAATCCGAGAGAAGCCAAAAGAA
CATTGGCAAGAGAACTGGTCGCCCTCTACCACTGCGATACTGATGCCGTCAAAGCAGAAGAACATTTCGACAGGGTGTTC
GTTCAGAAAAAGGCGCCTGAAAACATAGAGGTATTTACCTTCAGGTCCTCAACCATGCAGCTCGTCGAACTTCTTGGAGA
GCTTCAAGCCGTTGGATCAAAAAGTGAAGCCAGACGCCTGATTCAGCAGAATGCAATCCAGATTGATGACGAAAAAATCA
GCTCAATCGATTATGAAGTCGAACTGAACGACACGCCGAAAATCATCAAAGCAGGCAAAAGAAAATTTTTCAAAGTCATG
CAAAAAAAAGATTTTGAATGA

Upstream 100 bases:

>100_bases
GAAAAAAACTTTTCGATAAACGAGAGTCTATCAAAAACAGGGATGCGGAGCGTCAGCTGCAACGACTTAAACATCAATAT
TAAAAAAGCCTGACCTATTT

Downstream 100 bases:

>100_bases
GGGTGCTTTTTCCTATTATTGCTGAGTTTCAGCCTATCATTTATTAACCTTATCCGGAGGAGCAAGGCGTTGTCATTTGT
CCCTACTAAAGTGTTTTTCA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTFPPIKEQLDLIINNTVEVISEEELEKKLDKSIKTGKPLKVKLGADPSRPDLHLGHSVVLRKLRDFQDLGHEAILIIGD
FTAMIGDPSGKSKTRPQLSAQEARENGETYFEQASKILDPEKTTICYNSEWLGKMTFSDVIRLSSHYTVARMLERDDFER
RYQSCEPISIHEFLYPLAQGMDSVHLKNDIELGGTDQKFNLLVGRDLQREYHIDPQVCITMPLLVGTFGDEKMSKSLGNA
ISFTDSAQDVYGKTLSIPDSLIETWFKLLVPQPRQSIKLFMEIIRQNPREAKRTLARELVALYHCDTDAVKAEEHFDRVF
VQKKAPENIEVFTFRSSTMQLVELLGELQAVGSKSEARRLIQQNAIQIDDEKISSIDYEVELNDTPKIIKAGKRKFFKVM
QKKDFE

Sequences:

>Translated_406_residues
MTFPPIKEQLDLIINNTVEVISEEELEKKLDKSIKTGKPLKVKLGADPSRPDLHLGHSVVLRKLRDFQDLGHEAILIIGD
FTAMIGDPSGKSKTRPQLSAQEARENGETYFEQASKILDPEKTTICYNSEWLGKMTFSDVIRLSSHYTVARMLERDDFER
RYQSCEPISIHEFLYPLAQGMDSVHLKNDIELGGTDQKFNLLVGRDLQREYHIDPQVCITMPLLVGTFGDEKMSKSLGNA
ISFTDSAQDVYGKTLSIPDSLIETWFKLLVPQPRQSIKLFMEIIRQNPREAKRTLARELVALYHCDTDAVKAEEHFDRVF
VQKKAPENIEVFTFRSSTMQLVELLGELQAVGSKSEARRLIQQNAIQIDDEKISSIDYEVELNDTPKIIKAGKRKFFKVM
QKKDFE
>Mature_405_residues
TFPPIKEQLDLIINNTVEVISEEELEKKLDKSIKTGKPLKVKLGADPSRPDLHLGHSVVLRKLRDFQDLGHEAILIIGDF
TAMIGDPSGKSKTRPQLSAQEARENGETYFEQASKILDPEKTTICYNSEWLGKMTFSDVIRLSSHYTVARMLERDDFERR
YQSCEPISIHEFLYPLAQGMDSVHLKNDIELGGTDQKFNLLVGRDLQREYHIDPQVCITMPLLVGTFGDEKMSKSLGNAI
SFTDSAQDVYGKTLSIPDSLIETWFKLLVPQPRQSIKLFMEIIRQNPREAKRTLARELVALYHCDTDAVKAEEHFDRVFV
QKKAPENIEVFTFRSSTMQLVELLGELQAVGSKSEARRLIQQNAIQIDDEKISSIDYEVELNDTPKIIKAGKRKFFKVMQ
KKDFE

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=428, Percent_Identity=25.4672897196262, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1787925, Length=417, Percent_Identity=25.8992805755396, Blast_Score=115, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI25145802, Length=390, Percent_Identity=27.4358974358974, Blast_Score=98, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6325160, Length=425, Percent_Identity=26.8235294117647, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20130383, Length=400, Percent_Identity=25.5, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46412; Mature: 46281

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFPPIKEQLDLIINNTVEVISEEELEKKLDKSIKTGKPLKVKLGADPSRPDLHLGHSVV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHH
LRKLRDFQDLGHEAILIIGDFTAMIGDPSGKSKTRPQLSAQEARENGETYFEQASKILDP
HHHHHHHHHCCCCEEEEEECHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCC
EKTTICYNSEWLGKMTFSDVIRLSSHYTVARMLERDDFERRYQSCEPISIHEFLYPLAQG
CCCEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
MDSVHLKNDIELGGTDQKFNLLVGRDLQREYHIDPQVCITMPLLVGTFGDEKMSKSLGNA
CCCEECCCCCCCCCCCCEEEEEECCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCC
ISFTDSAQDVYGKTLSIPDSLIETWFKLLVPQPRQSIKLFMEIIRQNPREAKRTLARELV
EECCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
ALYHCDTDAVKAEEHFDRVFVQKKAPENIEVFTFRSSTMQLVELLGELQAVGSKSEARRL
HHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHH
IQQNAIQIDDEKISSIDYEVELNDTPKIIKAGKRKFFKVMQKKDFE
HHHCCCCCCCHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TFPPIKEQLDLIINNTVEVISEEELEKKLDKSIKTGKPLKVKLGADPSRPDLHLGHSVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHH
LRKLRDFQDLGHEAILIIGDFTAMIGDPSGKSKTRPQLSAQEARENGETYFEQASKILDP
HHHHHHHHHCCCCEEEEEECHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCC
EKTTICYNSEWLGKMTFSDVIRLSSHYTVARMLERDDFERRYQSCEPISIHEFLYPLAQG
CCCEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
MDSVHLKNDIELGGTDQKFNLLVGRDLQREYHIDPQVCITMPLLVGTFGDEKMSKSLGNA
CCCEECCCCCCCCCCCCEEEEEECCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCC
ISFTDSAQDVYGKTLSIPDSLIETWFKLLVPQPRQSIKLFMEIIRQNPREAKRTLARELV
EECCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
ALYHCDTDAVKAEEHFDRVFVQKKAPENIEVFTFRSSTMQLVELLGELQAVGSKSEARRL
HHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHH
IQQNAIQIDDEKISSIDYEVELNDTPKIIKAGKRKFFKVMQKKDFE
HHHCCCCCCCHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]