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The map label for this gene is smpB [H]

Identifier: 194333541

GI number: 194333541

Start: 753253

End: 753726

Strand: Reverse

Name: smpB [H]

Synonym: Paes_0707

Alternate gene names: 194333541

Gene position: 753726-753253 (Counterclockwise)

Preceding gene: 194333542

Following gene: 194333540

Centisome position: 29.99

GC content: 46.41

Gene sequence:

>474_bases
GTGGCTAAACAGCAACAGAAACCACAATATGTAAGCACCATCCTGAACCGCAAGGCAAGGCATGAGTTCACCATTCTCGA
AACAGTTGTGGCAGGCATTGAACTCCAGGGAAGCGAGGTTAAATCAATTCGCCTGGGACGAGCCAGTCTCAATGAAAGCT
ATGCCATCATTCACCGGGGTGAGGTATGGCTTGAAAACATGCAGATAACACCCTACGAGCACAATACCCTTGAACTGCCT
GATCCCAAACGCAGCCGGAAACTGCTTCTCCAGAAAAGCGAGATCATCAAAATCCAATCGAAACTGCATGAAAAAGGGCT
GACCCTGATCCCCCTGAAAGCGTTTTTTAACAGCCGCGGAATGCTGAAAATCGAACTGGGTATCGCAAAAGGAAAAAAAC
TTTTCGATAAACGAGAGTCTATCAAAAACAGGGATGCGGAGCGTCAGCTGCAACGACTTAAACATCAATATTAA

Upstream 100 bases:

>100_bases
GGTACGGATAAAAAAATACCTTGATCCGTTCCTTATGCTCAATATGGGGAACCTGATACCATTAGAATATTACGAAACCT
GTTGAAAACGGAAACAGAAC

Downstream 100 bases:

>100_bases
AAAAGCCTGACCTATTTATGACTTTTCCACCGATCAAAGAACAACTCGATCTCATTATCAATAACACCGTCGAGGTCATC
AGTGAAGAAGAGCTCGAAAA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MAKQQQKPQYVSTILNRKARHEFTILETVVAGIELQGSEVKSIRLGRASLNESYAIIHRGEVWLENMQITPYEHNTLELP
DPKRSRKLLLQKSEIIKIQSKLHEKGLTLIPLKAFFNSRGMLKIELGIAKGKKLFDKRESIKNRDAERQLQRLKHQY

Sequences:

>Translated_157_residues
MAKQQQKPQYVSTILNRKARHEFTILETVVAGIELQGSEVKSIRLGRASLNESYAIIHRGEVWLENMQITPYEHNTLELP
DPKRSRKLLLQKSEIIKIQSKLHEKGLTLIPLKAFFNSRGMLKIELGIAKGKKLFDKRESIKNRDAERQLQRLKHQY
>Mature_156_residues
AKQQQKPQYVSTILNRKARHEFTILETVVAGIELQGSEVKSIRLGRASLNESYAIIHRGEVWLENMQITPYEHNTLELPD
PKRSRKLLLQKSEIIKIQSKLHEKGLTLIPLKAFFNSRGMLKIELGIAKGKKLFDKRESIKNRDAERQLQRLKHQY

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=144, Percent_Identity=39.5833333333333, Blast_Score=110, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18285; Mature: 18154

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQQQKPQYVSTILNRKARHEFTILETVVAGIELQGSEVKSIRLGRASLNESYAIIHRG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECC
EVWLENMQITPYEHNTLELPDPKRSRKLLLQKSEIIKIQSKLHEKGLTLIPLKAFFNSRG
CEEEECCEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCC
MLKIELGIAKGKKLFDKRESIKNRDAERQLQRLKHQY
EEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
AKQQQKPQYVSTILNRKARHEFTILETVVAGIELQGSEVKSIRLGRASLNESYAIIHRG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECC
EVWLENMQITPYEHNTLELPDPKRSRKLLLQKSEIIKIQSKLHEKGLTLIPLKAFFNSRG
CEEEECCEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCC
MLKIELGIAKGKKLFDKRESIKNRDAERQLQRLKHQY
EEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]