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The map label for this gene is carB [H]

Identifier: 194333425

GI number: 194333425

Start: 621428

End: 623284

Strand: Reverse

Name: carB [H]

Synonym: Paes_0582

Alternate gene names: 194333425

Gene position: 623284-621428 (Counterclockwise)

Preceding gene: 194333429

Following gene: 194333422

Centisome position: 24.8

GC content: 51.43

Gene sequence:

>1857_bases
ATGATCACACCAGCTCATGACCTGCCGAGCGACGTCCTCAGCCTGGCCCGCAAAATATCCAGAGAACAACTCCTGAAAGC
CAAGGAAAATGGTTTTTCTGATTTCCAGCTCGCCAACATTTTCAATGCGACCGAGCCGGATATCCGTATGCTCAGAAAGC
ATTACGGTGTGGAATCGGTTTTCAAAACCGTCGATACCTGTGCAGCAGAGTTCGACGCATCAACACCCTATCACTACTCG
AGCTACGACGACGAAAACGAGTCTGTCCGTTCGGACAACAAGAAAGTCATCATTCTCGGCGGCGGACCGAACCGGATCGG
TCAGGGCATCGAGTTCGACTACTGCTGTGTTCAGGCGGTCTTCGCCCTCAGAGAAGCCGGGTATGAAACCATCATGGTCA
ACTGCAATCCTGAAACCGTTTCGACCGACTACGATATTGCCGACAAGCTGTATTTCGAGCCTCTCACCTTTGAAGATACT
ATCCGCATCATCGAACACGAGGACCCCCTCGGCGTCATCGTGAGCTTTGGAGGCCAGACCCCGCTGAAACTCTCATCAAA
GCTTGAAGAAGCCGGCGTTACCATTCTGGGAACATCATCGAAAGGCATTGACCTTGCCGAAGACAGAAAAAAGTTCGGGG
CCGTGCTGCGAGAACTCAACATTCCGCATCCCGATTACGATACCGCGATCAGCTACGAGGAAGCCCAGGAAATAACCCGC
AGGATCGGCTACCCAGTACTTGTCCGCCCAAGTTATGTTCTTGGCGGGCGGGCCATGAAAATCATCTACAGCGATGACTC
ACTCAAAGAGTATGTCGACCAGGCGCTGTTTATCACTGAAAAGTATCCGTTGCTCATCGACAGGTTCCTCGAAACTGCGG
TAGAGTTTGACATCGATGCCATTGCCGACACGACGGACTGCGTTGTCAGCGGCATCATGCAGCATGTCGAAGCGGCAGGT
ATCCACAGCGGAGACTCGACATCCATTCTCCCCTATCATAATATCAATCCCGAGGTCATCAAGACCATGAAGGAGTACAC
CCGAAGACTGGCCGAAAGAATAGGCGTCGTCGGGCTCATGAATGTCCAGTATGCCGTCCAGAACGACAGCGTCTACGTCA
TCGAAGTCAACCCGCGCGCCAGCCGTACGGTTCCGTTTGTCGGTAAAGCAACCGCCATTCCTGTCGTCAAAATAGCAACA
CGGGTCATGCTGGGAGAGAAACTTTCAGATCTGCGCAAAGAGTATCGTCTGGCCGACTGTGACGAGCTTGGCATGAACCA
CCTTGCCATCAAGGAACCGGTATTTCCTTTCTCGAAGTTCGTCAAATCAGGCGTCTATCTTGGTCCGGAAATGCGCTCGA
CAGGTGAAGCAATGAGCCTTGCCTATAATTTCCCTGAAGCCTTTGCAAAGGCTTACCAGGCAGCCAACATGGAACTGCCG
ACATCAGGAACAGTTTTCATCAGCGTCAATGACCACGATAAAGATGAGAGAATTATACGCATCGCAAAAGATCTCTACCG
TATAGGATTTGATCTGGTTGCCACCTCAGGCACGCAGCAATATCTGATAGAGCACGGTATCGAATGCAAAAAAGTCTATA
AAGTCGGAGAAGAAGGTCGGCCCAACATCTTTGATACAATACGTCTGGGCAAAATCGACTTTGTCATCAACACGCCTCTG
GGTGAGCGGGCACTGAACGACGAAGAAGCAATAGGTGCCGCCTCGGTCACAAGCGGAGTCCCATTTGTCACGACCATAGA
AGCCGCCGAAGCATCAATTCAGGCGATCGACTGCCTCAGACAACAAGAGTTTGGCGTCAAGAGTCTTCAGGAATACGCCA
GTTACCGCAATCAGTAA

Upstream 100 bases:

>100_bases
CTTTCACACATTATTGTCCTGCTTGATAGTATATTATAGTAATGTCCAGAACCGTTTCAACTGCTTTTTTACAAGCGACT
ATAAACCTTGACGACCCTAC

Downstream 100 bases:

>100_bases
GCGCTATCAAGCAGCTCAGTGAGAAACGCCACAACCTGAGTCTCAATCGCCGGTGCCGAACTCCTTCTCGGACCGGCGAT
ATTGAGTATTCTGATCCCCT

Product: carbamoyl-phosphate synthetase large chain domain-containing protein

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MITPAHDLPSDVLSLARKISREQLLKAKENGFSDFQLANIFNATEPDIRMLRKHYGVESVFKTVDTCAAEFDASTPYHYS
SYDDENESVRSDNKKVIILGGGPNRIGQGIEFDYCCVQAVFALREAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDT
IRIIEHEDPLGVIVSFGGQTPLKLSSKLEEAGVTILGTSSKGIDLAEDRKKFGAVLRELNIPHPDYDTAISYEEAQEITR
RIGYPVLVRPSYVLGGRAMKIIYSDDSLKEYVDQALFITEKYPLLIDRFLETAVEFDIDAIADTTDCVVSGIMQHVEAAG
IHSGDSTSILPYHNINPEVIKTMKEYTRRLAERIGVVGLMNVQYAVQNDSVYVIEVNPRASRTVPFVGKATAIPVVKIAT
RVMLGEKLSDLRKEYRLADCDELGMNHLAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLAYNFPEAFAKAYQAANMELP
TSGTVFISVNDHDKDERIIRIAKDLYRIGFDLVATSGTQQYLIEHGIECKKVYKVGEEGRPNIFDTIRLGKIDFVINTPL
GERALNDEEAIGAASVTSGVPFVTTIEAAEASIQAIDCLRQQEFGVKSLQEYASYRNQ

Sequences:

>Translated_618_residues
MITPAHDLPSDVLSLARKISREQLLKAKENGFSDFQLANIFNATEPDIRMLRKHYGVESVFKTVDTCAAEFDASTPYHYS
SYDDENESVRSDNKKVIILGGGPNRIGQGIEFDYCCVQAVFALREAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDT
IRIIEHEDPLGVIVSFGGQTPLKLSSKLEEAGVTILGTSSKGIDLAEDRKKFGAVLRELNIPHPDYDTAISYEEAQEITR
RIGYPVLVRPSYVLGGRAMKIIYSDDSLKEYVDQALFITEKYPLLIDRFLETAVEFDIDAIADTTDCVVSGIMQHVEAAG
IHSGDSTSILPYHNINPEVIKTMKEYTRRLAERIGVVGLMNVQYAVQNDSVYVIEVNPRASRTVPFVGKATAIPVVKIAT
RVMLGEKLSDLRKEYRLADCDELGMNHLAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLAYNFPEAFAKAYQAANMELP
TSGTVFISVNDHDKDERIIRIAKDLYRIGFDLVATSGTQQYLIEHGIECKKVYKVGEEGRPNIFDTIRLGKIDFVINTPL
GERALNDEEAIGAASVTSGVPFVTTIEAAEASIQAIDCLRQQEFGVKSLQEYASYRNQ
>Mature_618_residues
MITPAHDLPSDVLSLARKISREQLLKAKENGFSDFQLANIFNATEPDIRMLRKHYGVESVFKTVDTCAAEFDASTPYHYS
SYDDENESVRSDNKKVIILGGGPNRIGQGIEFDYCCVQAVFALREAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDT
IRIIEHEDPLGVIVSFGGQTPLKLSSKLEEAGVTILGTSSKGIDLAEDRKKFGAVLRELNIPHPDYDTAISYEEAQEITR
RIGYPVLVRPSYVLGGRAMKIIYSDDSLKEYVDQALFITEKYPLLIDRFLETAVEFDIDAIADTTDCVVSGIMQHVEAAG
IHSGDSTSILPYHNINPEVIKTMKEYTRRLAERIGVVGLMNVQYAVQNDSVYVIEVNPRASRTVPFVGKATAIPVVKIAT
RVMLGEKLSDLRKEYRLADCDELGMNHLAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLAYNFPEAFAKAYQAANMELP
TSGTVFISVNDHDKDERIIRIAKDLYRIGFDLVATSGTQQYLIEHGIECKKVYKVGEEGRPNIFDTIRLGKIDFVINTPL
GERALNDEEAIGAASVTSGVPFVTTIEAAEASIQAIDCLRQQEFGVKSLQEYASYRNQ

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ATP-grasp domains [H]

Homologues:

Organism=Homo sapiens, GI170295797, Length=605, Percent_Identity=35.8677685950413, Blast_Score=391, Evalue=1e-109,
Organism=Homo sapiens, GI169790915, Length=605, Percent_Identity=35.8677685950413, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI21361331, Length=605, Percent_Identity=35.8677685950413, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI18105007, Length=509, Percent_Identity=36.3457760314342, Blast_Score=350, Evalue=2e-96,
Organism=Escherichia coli, GI1786216, Length=576, Percent_Identity=48.6111111111111, Blast_Score=545, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI193204318, Length=596, Percent_Identity=34.8993288590604, Blast_Score=368, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6322331, Length=606, Percent_Identity=36.1386138613861, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6322569, Length=524, Percent_Identity=36.0687022900763, Blast_Score=331, Evalue=3e-91,
Organism=Drosophila melanogaster, GI24642586, Length=600, Percent_Identity=34.6666666666667, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI45555749, Length=516, Percent_Identity=37.5968992248062, Blast_Score=351, Evalue=7e-97,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006275
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR005480
- InterPro:   IPR011607
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02787 CPSase_L_D3; PF02142 MGS [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 68798; Mature: 68798

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50975 ATP_GRASP ; PS00962 RIBOSOMAL_S2_1 ; PS00866 CPSASE_1 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITPAHDLPSDVLSLARKISREQLLKAKENGFSDFQLANIFNATEPDIRMLRKHYGVESV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCHHHHHHHHHCCHHHH
FKTVDTCAAEFDASTPYHYSSYDDENESVRSDNKKVIILGGGPNRIGQGIEFDYCCVQAV
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCHHHHCCCCCHHHHHHHHH
FALREAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDTIRIIEHEDPLGVIVSFGGQT
HHHHHCCCEEEEEECCCCCCCCCCCHHHHHEECCCCHHHHEEEEECCCCCEEEEECCCCC
PLKLSSKLEEAGVTILGTSSKGIDLAEDRKKFGAVLRELNIPHPDYDTAISYEEAQEITR
CHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
RIGYPVLVRPSYVLGGRAMKIIYSDDSLKEYVDQALFITEKYPLLIDRFLETAVEFDIDA
HCCCCEEECCCEEECCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHH
IADTTDCVVSGIMQHVEAAGIHSGDSTSILPYHNINPEVIKTMKEYTRRLAERIGVVGLM
HCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEE
NVQYAVQNDSVYVIEVNPRASRTVPFVGKATAIPVVKIATRVMLGEKLSDLRKEYRLADC
EEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
DELGMNHLAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLAYNFPEAFAKAYQAANMELP
HHHCCCCEEECCCCCHHHHHHHCCCEECCCHHCCCCHHHEEECCHHHHHHHHHHCCCCCC
TSGTVFISVNDHDKDERIIRIAKDLYRIGFDLVATSGTQQYLIEHGIECKKVYKVGEEGR
CCCEEEEEECCCCCHHHHHHHHHHHHHHCHHHEECCCHHHHHHHCCCCHHHHHHCCCCCC
PNIFDTIRLGKIDFVINTPLGERALNDEEAIGAASVTSGVPFVTTIEAAEASIQAIDCLR
CCHHHEEEECEEEEEEECCCCCCCCCCHHHCCCHHHCCCCCEEEEEHHHHHHHHHHHHHH
QQEFGVKSLQEYASYRNQ
HHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MITPAHDLPSDVLSLARKISREQLLKAKENGFSDFQLANIFNATEPDIRMLRKHYGVESV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCHHHHHHHHHCCHHHH
FKTVDTCAAEFDASTPYHYSSYDDENESVRSDNKKVIILGGGPNRIGQGIEFDYCCVQAV
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCHHHHCCCCCHHHHHHHHH
FALREAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDTIRIIEHEDPLGVIVSFGGQT
HHHHHCCCEEEEEECCCCCCCCCCCHHHHHEECCCCHHHHEEEEECCCCCEEEEECCCCC
PLKLSSKLEEAGVTILGTSSKGIDLAEDRKKFGAVLRELNIPHPDYDTAISYEEAQEITR
CHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
RIGYPVLVRPSYVLGGRAMKIIYSDDSLKEYVDQALFITEKYPLLIDRFLETAVEFDIDA
HCCCCEEECCCEEECCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHH
IADTTDCVVSGIMQHVEAAGIHSGDSTSILPYHNINPEVIKTMKEYTRRLAERIGVVGLM
HCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEE
NVQYAVQNDSVYVIEVNPRASRTVPFVGKATAIPVVKIATRVMLGEKLSDLRKEYRLADC
EEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
DELGMNHLAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLAYNFPEAFAKAYQAANMELP
HHHCCCCEEECCCCCHHHHHHHCCCEECCCHHCCCCHHHEEECCHHHHHHHHHHCCCCCC
TSGTVFISVNDHDKDERIIRIAKDLYRIGFDLVATSGTQQYLIEHGIECKKVYKVGEEGR
CCCEEEEEECCCCCHHHHHHHHHHHHHHCHHHEECCCHHHHHHHCCCCHHHHHHCCCCCC
PNIFDTIRLGKIDFVINTPLGERALNDEEAIGAASVTSGVPFVTTIEAAEASIQAIDCLR
CCHHHEEEECEEEEEEECCCCCCCCCCHHHCCCHHHCCCCCEEEEEHHHHHHHHHHHHHH
QQEFGVKSLQEYASYRNQ
HHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]