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The map label for this gene is dnaG [H]

Identifier: 194333429

GI number: 194333429

Start: 626687

End: 628585

Strand: Reverse

Name: dnaG [H]

Synonym: Paes_0586

Alternate gene names: 194333429

Gene position: 628585-626687 (Counterclockwise)

Preceding gene: 194333435

Following gene: 194333425

Centisome position: 25.01

GC content: 51.13

Gene sequence:

>1899_bases
ATGATACCACAACAGAAAATCGATGAAATCCGTCAGAGTATCGACATTGTCGATATTATCGCAGATTATGTCAAACTCCA
GCCATCAGGAAGAAACTATAAAGCGCTCTCTCCGTTTACCGCTGAAAAAACCCCATCATTTGTCGTTTCCCCCGACAAGC
AGATTTACAAATGCTTCTCATCCGGCAAAGGAGGCAACGTTTTCACCTTCCTGATGGAAGTCGAAAAAATATCATTTCCC
GAAGCCATAGAAGTGGTCGCTAAACGTGCAGGCATCGATATCAGCCAGTACCGTCACACTTCTCAGGATAGAGACCAGAA
ACATAACAACGAATACGACACCCTGAAATGGGCAGGCAGGCTCTACAACCGACAGCTTCACTCCCCTTCTGGAAAAGAAG
GATTACACTATCTGGTCAATGAGCGAAAGTTATCTCAGAAAACCATCATGACCTTCGGAGTCGGCTATGCTCCGGACAGC
TGGGATTTTCTGACAGGAGAAGCAGGGCGTTCAGCAATACCCGTCGAGTCACTCTCCCTGACAGGCCTGCTCTATCACAA
CGCACGGAAAAACTCCTCCTACGACTACTTCCGTAACCGGATCATGTTCCCTATTTTCTCTGTCGCAGGACAGGTGATCG
GGTTCGGTGGACGAACGCTCAGCAGTGACCCCAACTCCCCGAAGTACCTCAATTCACCCGAAACACCTTTTTTCGACAAG
TCGAAAGTCCTTTACGGACTTCATGCCGCAAGGAACGAGATCCGCAGAAACGGACAGGCAATACTTGTCGAAGGCTATAT
GGATGTTCTGGCACTCCACCAGGCCGGCATCACGACAGCAGTCGCATCGTGCGGGACAGCTCTGACAAAATACCAGGCCG
CAATACTGCAGCGATATTGCAGTGAAGTACTCTTTCTCTATGACGCTGACGATGCAGGGCAGAAATCGATGCTTTCAGGC
ATAGACATCCTCGTCAGCTCCGGACTGACACCCTGGACCGCCACTCTGCCCGAAGGGGATGATCCTGACAGTTTCATACG
AAAACAGGGCAAAGAGGCGTTCCTCGACTACATCAAGGAAAACAGACTCTCGTTTATCGATTTTCAGCTTGCCTTTCACC
TGAAAAAAGGAGCGATGAACGAACCCCGCAACAAGGCGATCGCAGTTCGTTCGATGCTGAAAACAATCCATCTCGTTCAG
GACAGTATGCAGAAAGAACTCTACCTTCAGGAGCTGGAACAGAAGCTGGGTATCAGCATGGCTGTGCTCCGCGAAATCAT
GCTTCAGATGCGGGCCGGTCAGAAACAGGCAACCCAAAAGCCAGCTCCTCAGCACTCCGGAACCGACTCAGAGGAGCCCG
GCCTTCGGATTTCCGTGCAGGAAAAAACGTTTCTGAAAGCGTTGCTTGAGAGCGTCGAATACGGCAACGAAGTGCTTGAA
TTCGCATCTTCCCATCAGGAGCTGCTGCAGCTATCGAATCCTGCAGCGACCACCGTGCTCACCCATCTGCTCAACAGCTA
CCGCAATTCGATCGCGCATTCAGGAGCACATCTCGACGTGGCAGCCGAAATAGGCTCGATAGAGGACGCCGACGCAAGAG
GCCTCGCCTCCGGACTGCTCATCGACCCCCCTGTCAGCGCCCACTGGCAGGCATCATTTGATGAGGAACAGCAAAAAGCA
AAGCGCTGCCTGACAGCGTTTCTGGACTCCTTCAAGCACCTGATTCTCAATCATTTCCGCCAGCAGAAGACAAATCTGAT
AAGCCGGTTACGCTCTGCAGCCAATCTTGAAGAAGAAAAAAGCCTGAGCTCGGATCTCGCCCTCCTGGTCAGGGAAGAAC
AGAAAACGGAAAACGAAGTAAACGCGATGATCCGCTCCATTTTAAACCCAAACGGCTGA

Upstream 100 bases:

>100_bases
ACAACGCTGATCTGCGTTGCGAAATGCATTTTTTCAAGCTATCATACCTTCAAACTGAACACTCTGCCTTTTGCAGACCT
CAGCCTCGATGCCGAAACCA

Downstream 100 bases:

>100_bases
AAGGGTCAGTCCAGTTTGCGCAAACAGGGCTCAATGCCACAAACCACCCCCGCATCGTCTCCCGCGAAACGTCGCCTGAT
CCTCTTTCTCAACATCACAG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MIPQQKIDEIRQSIDIVDIIADYVKLQPSGRNYKALSPFTAEKTPSFVVSPDKQIYKCFSSGKGGNVFTFLMEVEKISFP
EAIEVVAKRAGIDISQYRHTSQDRDQKHNNEYDTLKWAGRLYNRQLHSPSGKEGLHYLVNERKLSQKTIMTFGVGYAPDS
WDFLTGEAGRSAIPVESLSLTGLLYHNARKNSSYDYFRNRIMFPIFSVAGQVIGFGGRTLSSDPNSPKYLNSPETPFFDK
SKVLYGLHAARNEIRRNGQAILVEGYMDVLALHQAGITTAVASCGTALTKYQAAILQRYCSEVLFLYDADDAGQKSMLSG
IDILVSSGLTPWTATLPEGDDPDSFIRKQGKEAFLDYIKENRLSFIDFQLAFHLKKGAMNEPRNKAIAVRSMLKTIHLVQ
DSMQKELYLQELEQKLGISMAVLREIMLQMRAGQKQATQKPAPQHSGTDSEEPGLRISVQEKTFLKALLESVEYGNEVLE
FASSHQELLQLSNPAATTVLTHLLNSYRNSIAHSGAHLDVAAEIGSIEDADARGLASGLLIDPPVSAHWQASFDEEQQKA
KRCLTAFLDSFKHLILNHFRQQKTNLISRLRSAANLEEEKSLSSDLALLVREEQKTENEVNAMIRSILNPNG

Sequences:

>Translated_632_residues
MIPQQKIDEIRQSIDIVDIIADYVKLQPSGRNYKALSPFTAEKTPSFVVSPDKQIYKCFSSGKGGNVFTFLMEVEKISFP
EAIEVVAKRAGIDISQYRHTSQDRDQKHNNEYDTLKWAGRLYNRQLHSPSGKEGLHYLVNERKLSQKTIMTFGVGYAPDS
WDFLTGEAGRSAIPVESLSLTGLLYHNARKNSSYDYFRNRIMFPIFSVAGQVIGFGGRTLSSDPNSPKYLNSPETPFFDK
SKVLYGLHAARNEIRRNGQAILVEGYMDVLALHQAGITTAVASCGTALTKYQAAILQRYCSEVLFLYDADDAGQKSMLSG
IDILVSSGLTPWTATLPEGDDPDSFIRKQGKEAFLDYIKENRLSFIDFQLAFHLKKGAMNEPRNKAIAVRSMLKTIHLVQ
DSMQKELYLQELEQKLGISMAVLREIMLQMRAGQKQATQKPAPQHSGTDSEEPGLRISVQEKTFLKALLESVEYGNEVLE
FASSHQELLQLSNPAATTVLTHLLNSYRNSIAHSGAHLDVAAEIGSIEDADARGLASGLLIDPPVSAHWQASFDEEQQKA
KRCLTAFLDSFKHLILNHFRQQKTNLISRLRSAANLEEEKSLSSDLALLVREEQKTENEVNAMIRSILNPNG
>Mature_632_residues
MIPQQKIDEIRQSIDIVDIIADYVKLQPSGRNYKALSPFTAEKTPSFVVSPDKQIYKCFSSGKGGNVFTFLMEVEKISFP
EAIEVVAKRAGIDISQYRHTSQDRDQKHNNEYDTLKWAGRLYNRQLHSPSGKEGLHYLVNERKLSQKTIMTFGVGYAPDS
WDFLTGEAGRSAIPVESLSLTGLLYHNARKNSSYDYFRNRIMFPIFSVAGQVIGFGGRTLSSDPNSPKYLNSPETPFFDK
SKVLYGLHAARNEIRRNGQAILVEGYMDVLALHQAGITTAVASCGTALTKYQAAILQRYCSEVLFLYDADDAGQKSMLSG
IDILVSSGLTPWTATLPEGDDPDSFIRKQGKEAFLDYIKENRLSFIDFQLAFHLKKGAMNEPRNKAIAVRSMLKTIHLVQ
DSMQKELYLQELEQKLGISMAVLREIMLQMRAGQKQATQKPAPQHSGTDSEEPGLRISVQEKTFLKALLESVEYGNEVLE
FASSHQELLQLSNPAATTVLTHLLNSYRNSIAHSGAHLDVAAEIGSIEDADARGLASGLLIDPPVSAHWQASFDEEQQKA
KRCLTAFLDSFKHLILNHFRQQKTNLISRLRSAANLEEEKSLSSDLALLVREEQKTENEVNAMIRSILNPNG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=519, Percent_Identity=34.2967244701349, Blast_Score=253, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 70617; Mature: 70617

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPQQKIDEIRQSIDIVDIIADYVKLQPSGRNYKALSPFTAEKTPSFVVSPDKQIYKCFS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHH
SGKGGNVFTFLMEVEKISFPEAIEVVAKRAGIDISQYRHTSQDRDQKHNNEYDTLKWAGR
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHHCCCCHHHHHHHHH
LYNRQLHSPSGKEGLHYLVNERKLSQKTIMTFGVGYAPDSWDFLTGEAGRSAIPVESLSL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCHHHHHH
TGLLYHNARKNSSYDYFRNRIMFPIFSVAGQVIGFGGRTLSSDPNSPKYLNSPETPFFDK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
SKVLYGLHAARNEIRRNGQAILVEGYMDVLALHQAGITTAVASCGTALTKYQAAILQRYC
CHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEVLFLYDADDAGQKSMLSGIDILVSSGLTPWTATLPEGDDPDSFIRKQGKEAFLDYIKE
HHHEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHCCHHHHHHHHHH
NRLSFIDFQLAFHLKKGAMNEPRNKAIAVRSMLKTIHLVQDSMQKELYLQELEQKLGISM
CCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AVLREIMLQMRAGQKQATQKPAPQHSGTDSEEPGLRISVQEKTFLKALLESVEYGNEVLE
HHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
FASSHQELLQLSNPAATTVLTHLLNSYRNSIAHSGAHLDVAAEIGSIEDADARGLASGLL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCHHHHHCCCE
IDPPVSAHWQASFDEEQQKAKRCLTAFLDSFKHLILNHFRQQKTNLISRLRSAANLEEEK
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SLSSDLALLVREEQKTENEVNAMIRSILNPNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIPQQKIDEIRQSIDIVDIIADYVKLQPSGRNYKALSPFTAEKTPSFVVSPDKQIYKCFS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHH
SGKGGNVFTFLMEVEKISFPEAIEVVAKRAGIDISQYRHTSQDRDQKHNNEYDTLKWAGR
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHHCCCCHHHHHHHHH
LYNRQLHSPSGKEGLHYLVNERKLSQKTIMTFGVGYAPDSWDFLTGEAGRSAIPVESLSL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCHHHHHH
TGLLYHNARKNSSYDYFRNRIMFPIFSVAGQVIGFGGRTLSSDPNSPKYLNSPETPFFDK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
SKVLYGLHAARNEIRRNGQAILVEGYMDVLALHQAGITTAVASCGTALTKYQAAILQRYC
CHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEVLFLYDADDAGQKSMLSGIDILVSSGLTPWTATLPEGDDPDSFIRKQGKEAFLDYIKE
HHHEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHCCHHHHHHHHHH
NRLSFIDFQLAFHLKKGAMNEPRNKAIAVRSMLKTIHLVQDSMQKELYLQELEQKLGISM
CCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AVLREIMLQMRAGQKQATQKPAPQHSGTDSEEPGLRISVQEKTFLKALLESVEYGNEVLE
HHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
FASSHQELLQLSNPAATTVLTHLLNSYRNSIAHSGAHLDVAAEIGSIEDADARGLASGLL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCHHHHHCCCE
IDPPVSAHWQASFDEEQQKAKRCLTAFLDSFKHLILNHFRQQKTNLISRLRSAANLEEEK
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SLSSDLALLVREEQKTENEVNAMIRSILNPNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]