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The map label for this gene is pfp [H]

Identifier: 194333393

GI number: 194333393

Start: 593009

End: 594214

Strand: Reverse

Name: pfp [H]

Synonym: Paes_0550

Alternate gene names: 194333393

Gene position: 594214-593009 (Counterclockwise)

Preceding gene: 194333395

Following gene: 194333392

Centisome position: 23.65

GC content: 51.49

Gene sequence:

>1206_bases
GTGAAAAAAATTGGCATTCTCACCAGCGGAGGAGACTGCGGCGGTCTCAACGCAGTCATCAAAGGGGCAGCATTCATGGC
CCTCTCCAAAGGGATTGAAATGTATATTATCCCCAACGGCTACGCAGGACTATACAACCTTATCGACAATGAAAAACTCG
TACTGCTCGACCAGGCACGTCTGGATCAGTTTTCCGGAAACTTCGCAGGTTCGGAAGCAGGCCATTCGAGGGTGAAAATC
AAAGCCATCAGTAATCCCGAGAAGTACAACCGCATCAAAGAGGGCATGAAAAAGTTCGAACTCGATGGCCTCGTGGTCAG
CGGAGGTGATGATTCGGGTAGTGTCATGGTGGACCTGAACAGCAACGGAATTCACTGTATCCATGCCCCAAAAACCATGG
ATCTCGACCTGCAGACCTACTCCGTCGGTGGAGACTCGACCATCAACCGTATCGCCCAGTTCGTTCAGGACCTGAAAACC
ACCGGCCGTACCCATAACAGAATTCTTGTCACCGAAGTATTCGGACGTTATGCCGGCCACACCGCCTTCCGAAGCGGTAT
CGCCGCCGAAGCCGACTGTATCCTCATCCCTGAAATCCCGGTCGACTGGGATGTCGTCTACGAACACATTGTCGGAAGAT
TCACTCGCCGCATCCGCCAGAGTGACGTCAGCGCAGGCACCTATACCATCGTCGTCGCTGAAGGCATGAAAAACGCCGAC
GGTACGGACATTATCGATGAATCTGCAGGTGTTGATGCCTTTGGCCATCAAAAACTTGCCGGCGCCGGTAAATATGTGTG
CCAGGAGATGAAAAAGAGGTTAAAAGCCGACCCGGATATGCCAATTTTCATGAAAGAAACCGGCATGTTCGTCGAAGGCA
TCTACGAAATCCCCGAAATCCGTGAAATTCATCCGGGTCATCTTGTCCGTTCAGGAAACACCTCTGCCTATGACGTCAAC
TTCGGCTATGAAGCAGGAGCAGCAGCGGTCATACTCCTTTCCGAAGGCAAATCCGGTGTAACCATTTCCAAAGTAAAAGG
ACGCAAAATCGAATACATTGAATCCACCAAGGCCATCGAACAGCGTTTTGTCGACCTTGACCAGGTAGCGCTGTATGAGT
CGATCGGGGTCTGTTTCGGGCGTCATGTCGCCGCTTATGAGCCTATCCTCAGAGAGGTTGACGGGGTCTATGACAGGATT
TACTGA

Upstream 100 bases:

>100_bases
GCTATGAATTCAGTTGAAAAATCAGACCGCATCATCTATCTTGACATACAGTAATTCAACCCTACATCACTAATCTGACT
ATCGACAGGAGATACACACC

Downstream 100 bases:

>100_bases
CAATCCATTCCCGGAGCCTCACACCAATCACATTACGAGAGGTCCCGCCCGAAAATGCGGGACCTTTCTTTATGTAACCG
GCCGCTGCTTTTTTGCCTCA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKKIGILTSGGDCGGLNAVIKGAAFMALSKGIEMYIIPNGYAGLYNLIDNEKLVLLDQARLDQFSGNFAGSEAGHSRVKI
KAISNPEKYNRIKEGMKKFELDGLVVSGGDDSGSVMVDLNSNGIHCIHAPKTMDLDLQTYSVGGDSTINRIAQFVQDLKT
TGRTHNRILVTEVFGRYAGHTAFRSGIAAEADCILIPEIPVDWDVVYEHIVGRFTRRIRQSDVSAGTYTIVVAEGMKNAD
GTDIIDESAGVDAFGHQKLAGAGKYVCQEMKKRLKADPDMPIFMKETGMFVEGIYEIPEIREIHPGHLVRSGNTSAYDVN
FGYEAGAAAVILLSEGKSGVTISKVKGRKIEYIESTKAIEQRFVDLDQVALYESIGVCFGRHVAAYEPILREVDGVYDRI
Y

Sequences:

>Translated_401_residues
MKKIGILTSGGDCGGLNAVIKGAAFMALSKGIEMYIIPNGYAGLYNLIDNEKLVLLDQARLDQFSGNFAGSEAGHSRVKI
KAISNPEKYNRIKEGMKKFELDGLVVSGGDDSGSVMVDLNSNGIHCIHAPKTMDLDLQTYSVGGDSTINRIAQFVQDLKT
TGRTHNRILVTEVFGRYAGHTAFRSGIAAEADCILIPEIPVDWDVVYEHIVGRFTRRIRQSDVSAGTYTIVVAEGMKNAD
GTDIIDESAGVDAFGHQKLAGAGKYVCQEMKKRLKADPDMPIFMKETGMFVEGIYEIPEIREIHPGHLVRSGNTSAYDVN
FGYEAGAAAVILLSEGKSGVTISKVKGRKIEYIESTKAIEQRFVDLDQVALYESIGVCFGRHVAAYEPILREVDGVYDRI
Y
>Mature_401_residues
MKKIGILTSGGDCGGLNAVIKGAAFMALSKGIEMYIIPNGYAGLYNLIDNEKLVLLDQARLDQFSGNFAGSEAGHSRVKI
KAISNPEKYNRIKEGMKKFELDGLVVSGGDDSGSVMVDLNSNGIHCIHAPKTMDLDLQTYSVGGDSTINRIAQFVQDLKT
TGRTHNRILVTEVFGRYAGHTAFRSGIAAEADCILIPEIPVDWDVVYEHIVGRFTRRIRQSDVSAGTYTIVVAEGMKNAD
GTDIIDESAGVDAFGHQKLAGAGKYVCQEMKKRLKADPDMPIFMKETGMFVEGIYEIPEIREIHPGHLVRSGNTSAYDVN
FGYEAGAAAVILLSEGKSGVTISKVKGRKIEYIESTKAIEQRFVDLDQVALYESIGVCFGRHVAAYEPILREVDGVYDRI
Y

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=362, Percent_Identity=25.9668508287293, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI266453748, Length=362, Percent_Identity=25.9668508287293, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI4505749, Length=362, Percent_Identity=25.9668508287293, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI48762920, Length=412, Percent_Identity=25.2427184466019, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI266453619, Length=362, Percent_Identity=25.9668508287293, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1790350, Length=370, Percent_Identity=28.6486486486486, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17558788, Length=399, Percent_Identity=26.5664160401003, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652337, Length=431, Percent_Identity=24.8259860788863, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17647809, Length=431, Percent_Identity=24.8259860788863, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI28573326, Length=429, Percent_Identity=24.4755244755245, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 43778; Mature: 43778

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGILTSGGDCGGLNAVIKGAAFMALSKGIEMYIIPNGYAGLYNLIDNEKLVLLDQAR
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCEEEEEEHHH
LDQFSGNFAGSEAGHSRVKIKAISNPEKYNRIKEGMKKFELDGLVVSGGDDSGSVMVDLN
HHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHEEECCEEEECCCCCCEEEEEEC
SNGIHCIHAPKTMDLDLQTYSVGGDSTINRIAQFVQDLKTTGRTHNRILVTEVFGRYAGH
CCCEEEEECCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCH
TAFRSGIAAEADCILIPEIPVDWDVVYEHIVGRFTRRIRQSDVSAGTYTIVVAEGMKNAD
HHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
GTDIIDESAGVDAFGHQKLAGAGKYVCQEMKKRLKADPDMPIFMKETGMFVEGIYEIPEI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHCCCCC
REIHPGHLVRSGNTSAYDVNFGYEAGAAAVILLSEGKSGVTISKVKGRKIEYIESTKAIE
CCCCCCCEEECCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCEEEHHHHHHHHH
QRFVDLDQVALYESIGVCFGRHVAAYEPILREVDGVYDRIY
HHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIGILTSGGDCGGLNAVIKGAAFMALSKGIEMYIIPNGYAGLYNLIDNEKLVLLDQAR
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCEEEEEEHHH
LDQFSGNFAGSEAGHSRVKIKAISNPEKYNRIKEGMKKFELDGLVVSGGDDSGSVMVDLN
HHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHEEECCEEEECCCCCCEEEEEEC
SNGIHCIHAPKTMDLDLQTYSVGGDSTINRIAQFVQDLKTTGRTHNRILVTEVFGRYAGH
CCCEEEEECCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCH
TAFRSGIAAEADCILIPEIPVDWDVVYEHIVGRFTRRIRQSDVSAGTYTIVVAEGMKNAD
HHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
GTDIIDESAGVDAFGHQKLAGAGKYVCQEMKKRLKADPDMPIFMKETGMFVEGIYEIPEI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHCCCCC
REIHPGHLVRSGNTSAYDVNFGYEAGAAAVILLSEGKSGVTISKVKGRKIEYIESTKAIE
CCCCCCCEEECCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCEEEHHHHHHHHH
QRFVDLDQVALYESIGVCFGRHVAAYEPILREVDGVYDRIY
HHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]