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The map label for this gene is ispF

Identifier: 194333395

GI number: 194333395

Start: 595866

End: 596339

Strand: Reverse

Name: ispF

Synonym: Paes_0552

Alternate gene names: 194333395

Gene position: 596339-595866 (Counterclockwise)

Preceding gene: 194333396

Following gene: 194333393

Centisome position: 23.73

GC content: 53.59

Gene sequence:

>474_bases
ATGCGCGTAGGTATAGGCATTGACGTCCATCAGTTTGTCGAAAACAGAAAACTTATCATCGGCGGAATCGAAATCCCGCA
CACGATGGGGCTGAAAGGCCACAGCGATGCAGACGTGCTGCTGCACGCCATCAGCGATGCCCTTCTGGGCGCAGCCGCGC
TGGGCGACATCGGCAAGCATTTCCCCGATACCAGCCCTGATTTCAAAGATATCGACAGCAGAATACTCCTGCGTCATGTC
GGCAAACTCATCGCTGACGAAGGCTACACCATCGTCAATATCGACTCGATGCTGCTCATGGAACGACCAAAAGTCGCGCC
CTACATTGTGGCTATGAGAGAAAGTATCGCCGAATGCCTGGATATCGAGACGAGCCGGGTATCAGTCAAGGCAACCACAA
ACGAAAAACTCGGCTACATCGGTCGCCAGGAAGGCGCCGCAGCTCACGCAGTCTGCCTGATTGAAGAAAAATAA

Upstream 100 bases:

>100_bases
AAGCAAAAGAGCAGACGGTTGCGTGTTCTCTTTTTCTGGAAAATAGTGCTATCTTTTGCCCACATGAATCACTCACCATA
ACCCATGCAATTCTACTCTC

Downstream 100 bases:

>100_bases
TCAGTGACCAGTGATCGGTGATCGGTGACCAGTGACCAGTGGTCGGTGACCAGTGATCGGTGATCGGTGACTGCGAACTG
GAAAAAAAAGCTGATTGTCT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRVGIGIDVHQFVENRKLIIGGIEIPHTMGLKGHSDADVLLHAISDALLGAAALGDIGKHFPDTSPDFKDIDSRILLRHV
GKLIADEGYTIVNIDSMLLMERPKVAPYIVAMRESIAECLDIETSRVSVKATTNEKLGYIGRQEGAAAHAVCLIEEK

Sequences:

>Translated_157_residues
MRVGIGIDVHQFVENRKLIIGGIEIPHTMGLKGHSDADVLLHAISDALLGAAALGDIGKHFPDTSPDFKDIDSRILLRHV
GKLIADEGYTIVNIDSMLLMERPKVAPYIVAMRESIAECLDIETSRVSVKATTNEKLGYIGRQEGAAAHAVCLIEEK
>Mature_157_residues
MRVGIGIDVHQFVENRKLIIGGIEIPHTMGLKGHSDADVLLHAISDALLGAAALGDIGKHFPDTSPDFKDIDSRILLRHV
GKLIADEGYTIVNIDSMLLMERPKVAPYIVAMRESIAECLDIETSRVSVKATTNEKLGYIGRQEGAAAHAVCLIEEK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=55.1948051948052, Blast_Score=153, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_PROA2 (B4S5L1)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002015255.1
- ProteinModelPortal:   B4S5L1
- SMR:   B4S5L1
- GeneID:   6458656
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_0552
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17053; Mature: 17053

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGIGIDVHQFVENRKLIIGGIEIPHTMGLKGHSDADVLLHAISDALLGAAALGDIGKH
CEECCCCCHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FPDTSPDFKDIDSRILLRHVGKLIADEGYTIVNIDSMLLMERPKVAPYIVAMRESIAECL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHEECCCCCCCHHHHHHHHHHHHHH
DIETSRVSVKATTNEKLGYIGRQEGAAAHAVCLIEEK
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MRVGIGIDVHQFVENRKLIIGGIEIPHTMGLKGHSDADVLLHAISDALLGAAALGDIGKH
CEECCCCCHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FPDTSPDFKDIDSRILLRHVGKLIADEGYTIVNIDSMLLMERPKVAPYIVAMRESIAECL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHEECCCCCCCHHHHHHHHHHHHHH
DIETSRVSVKATTNEKLGYIGRQEGAAAHAVCLIEEK
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA