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The map label for this gene is ilvE [H]

Identifier: 194333392

GI number: 194333392

Start: 591901

End: 592812

Strand: Reverse

Name: ilvE [H]

Synonym: Paes_0549

Alternate gene names: 194333392

Gene position: 592812-591901 (Counterclockwise)

Preceding gene: 194333393

Following gene: 194333391

Centisome position: 23.59

GC content: 50.33

Gene sequence:

>912_bases
ATGAACAAGTCCGAAAAAATCTGGATGAACGGGGAACTGGTTAACTGGCACGACGCCAAAATTCATATTCTTTCCCACGT
CATTCATTATGGTTCTTCCACGTTTGAAGGGATCCGATGCTATGACACCGACAAAGGCTCTGCAATACTGTTTTTTGACG
AACATATCAAGCGCCTCTATCAGTCATCCAAGATATACCGTATGGAAATTCCATACTCGGAACAGGAGCTGAAAGACGCC
ATTATCGCCACCATCAAAGCAAACGGTCATAAAGCATGCTATATCAGACCGCTGGTCAAACGCGGTCAGGGCGCGCTCGG
CGTCAATCCGGCCCTTGCATCGATTGAAGTTGCCATTGCAACCTGGGAATGGGGCTCCTACCTCGGCGAAGAGGTCCTCG
AGAACGGTGTTGATGTCTGCGTGTCATCCTGGGCAAGATTAGCGCCGAATACGCTTCCCACCTGGGCTAAAGCGGGGGGC
AACTACCTCAACTCCCAGCTTATCAAGATGGAAGCCCTGATGGATAATTATGCCGAGGGAATCGGTCTCGACGTCCACGG
CTACGTCGCCGAGGGTAGCGGTGAAAACATTTTTGTCATTCGTGACGGCATTATCTACACGCCGATGTCGGGTCAGTCTA
TCCTGCCGGGCATCACTCGCAATGCCGTTATTCATATCGCCAGAGAGCTTGGCTACGAGGTTCAGGAAACCCTGATCCCA
CGAGAAGCGCTCTATATTGCCGATGAGGTTTTTCTGACTGGAACGGCTGCTGAAATCACACCGGTCAGAAGTATCGACAA
ATACGCTGTAGGAGATGAAAAACGCGGCCCAATTACCGAGCTGCTGCAATCAAAATATCTCGACATCGTCAAAAAAGGGA
CCGATCCCTACAACTGGCTCACCTTCATATAA

Upstream 100 bases:

>100_bases
CTCACTGCCGGGAAAATCCCCTGCAGCGGCTTTTAAAAAACGTAAAACAATCTATCTTTGAAGATTGTATTCAGGTATTT
TTCACATCAATGAGCGAGCA

Downstream 100 bases:

>100_bases
GAAGTGATGCACTGCTGATGCCAGGGGGCGCACCGTTTTCTGTGCGCCCTGCTGTTGTATATCGACAAATCACCATCTCA
AAGAAACGGTGCTGAAGAGG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNKSEKIWMNGELVNWHDAKIHILSHVIHYGSSTFEGIRCYDTDKGSAILFFDEHIKRLYQSSKIYRMEIPYSEQELKDA
IIATIKANGHKACYIRPLVKRGQGALGVNPALASIEVAIATWEWGSYLGEEVLENGVDVCVSSWARLAPNTLPTWAKAGG
NYLNSQLIKMEALMDNYAEGIGLDVHGYVAEGSGENIFVIRDGIIYTPMSGQSILPGITRNAVIHIARELGYEVQETLIP
REALYIADEVFLTGTAAEITPVRSIDKYAVGDEKRGPITELLQSKYLDIVKKGTDPYNWLTFI

Sequences:

>Translated_303_residues
MNKSEKIWMNGELVNWHDAKIHILSHVIHYGSSTFEGIRCYDTDKGSAILFFDEHIKRLYQSSKIYRMEIPYSEQELKDA
IIATIKANGHKACYIRPLVKRGQGALGVNPALASIEVAIATWEWGSYLGEEVLENGVDVCVSSWARLAPNTLPTWAKAGG
NYLNSQLIKMEALMDNYAEGIGLDVHGYVAEGSGENIFVIRDGIIYTPMSGQSILPGITRNAVIHIARELGYEVQETLIP
REALYIADEVFLTGTAAEITPVRSIDKYAVGDEKRGPITELLQSKYLDIVKKGTDPYNWLTFI
>Mature_303_residues
MNKSEKIWMNGELVNWHDAKIHILSHVIHYGSSTFEGIRCYDTDKGSAILFFDEHIKRLYQSSKIYRMEIPYSEQELKDA
IIATIKANGHKACYIRPLVKRGQGALGVNPALASIEVAIATWEWGSYLGEEVLENGVDVCVSSWARLAPNTLPTWAKAGG
NYLNSQLIKMEALMDNYAEGIGLDVHGYVAEGSGENIFVIRDGIIYTPMSGQSILPGITRNAVIHIARELGYEVQETLIP
REALYIADEVFLTGTAAEITPVRSIDKYAVGDEKRGPITELLQSKYLDIVKKGTDPYNWLTFI

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=301, Percent_Identity=52.1594684385382, Blast_Score=330, Evalue=5e-92,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33800; Mature: 33800

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSEKIWMNGELVNWHDAKIHILSHVIHYGSSTFEGIRCYDTDKGSAILFFDEHIKRLY
CCCCCEEEECCCEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEHHHHHHHH
QSSKIYRMEIPYSEQELKDAIIATIKANGHKACYIRPLVKRGQGALGVNPALASIEVAIA
HCCCEEEEECCCCHHHHHHHEEEEEECCCCCEEEEEHHHHCCCCCCCCCCCCEEEEEEEE
TWEWGSYLGEEVLENGVDVCVSSWARLAPNTLPTWAKAGGNYLNSQLIKMEALMDNYAEG
EECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHC
IGLDVHGYVAEGSGENIFVIRDGIIYTPMSGQSILPGITRNAVIHIARELGYEVQETLIP
CCCEEEEEEEECCCCEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHCC
REALYIADEVFLTGTAAEITPVRSIDKYAVGDEKRGPITELLQSKYLDIVKKGTDPYNWL
HHHHEEEHHEEEECCCHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEE
TFI
EEC
>Mature Secondary Structure
MNKSEKIWMNGELVNWHDAKIHILSHVIHYGSSTFEGIRCYDTDKGSAILFFDEHIKRLY
CCCCCEEEECCCEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEHHHHHHHH
QSSKIYRMEIPYSEQELKDAIIATIKANGHKACYIRPLVKRGQGALGVNPALASIEVAIA
HCCCEEEEECCCCHHHHHHHEEEEEECCCCCEEEEEHHHHCCCCCCCCCCCCEEEEEEEE
TWEWGSYLGEEVLENGVDVCVSSWARLAPNTLPTWAKAGGNYLNSQLIKMEALMDNYAEG
EECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHC
IGLDVHGYVAEGSGENIFVIRDGIIYTPMSGQSILPGITRNAVIHIARELGYEVQETLIP
CCCEEEEEEEECCCCEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHCC
REALYIADEVFLTGTAAEITPVRSIDKYAVGDEKRGPITELLQSKYLDIVKKGTDPYNWL
HHHHEEEHHEEEECCCHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEE
TFI
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]