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The map label for this gene is rnhA

Identifier: 194333386

GI number: 194333386

Start: 586194

End: 586634

Strand: Reverse

Name: rnhA

Synonym: Paes_0543

Alternate gene names: 194333386

Gene position: 586634-586194 (Counterclockwise)

Preceding gene: 194333387

Following gene: 194333385

Centisome position: 23.34

GC content: 52.83

Gene sequence:

>441_bases
ATGAGAAAAAAAATCATCATCTATACCGACGGTGCGTGCAGCGGCAATCCGGGCAAAGGCGGATGGGGCGCACTGTTGAT
GTTCGGAGAGCTCAATCGTGAAATCTCAGGCTACTCGCCTGCCACGACAAACAACAGGATGGAGCTCATGGCCGCAATCC
AGGCTCTTGAGGCTCTCAAGGAACCCTGCGACGTGGACCTGTACAGCGATTCGAGCTATCTGGTCAACGCCATCAAGCTC
GGCTGGCTCAAGAAATGGTCCTCCGGCGGCTGGACAACCGCGTCCAGAAAACCAGTGGAAAATCAGGACCTCTGGAAAAA
GATTCTCCAGTTGATTAAATTGCACAACGTAACGTTCCACAAGGTCAAGGGCCATAGCGACAACGAATACAACAACCGCT
GCGACTATCTCGCCCGACAGGCCATCAAAAACAACCGGTAA

Upstream 100 bases:

>100_bases
ACTTGCAAAACTTTTCAAGTAACCACGACGTTTGCTGTCTGCTCACAGAAAGTGTTTTTTACAGGGCTCAATTTTTCGGG
ATTGAGCCTTTTTTTTGTCT

Downstream 100 bases:

>100_bases
TGGCAGATCATATGCAGAGCCCACCCACAAAAGCTCCTGATAGCAGCACAACCAGACCGCCCAGAAAAAAACCCAGCCTG
CTGGTTATCACCGTCATTCT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MRKKIIIYTDGACSGNPGKGGWGALLMFGELNREISGYSPATTNNRMELMAAIQALEALKEPCDVDLYSDSSYLVNAIKL
GWLKKWSSGGWTTASRKPVENQDLWKKILQLIKLHNVTFHKVKGHSDNEYNNRCDYLARQAIKNNR

Sequences:

>Translated_146_residues
MRKKIIIYTDGACSGNPGKGGWGALLMFGELNREISGYSPATTNNRMELMAAIQALEALKEPCDVDLYSDSSYLVNAIKL
GWLKKWSSGGWTTASRKPVENQDLWKKILQLIKLHNVTFHKVKGHSDNEYNNRCDYLARQAIKNNR
>Mature_146_residues
MRKKIIIYTDGACSGNPGKGGWGALLMFGELNREISGYSPATTNNRMELMAAIQALEALKEPCDVDLYSDSSYLVNAIKL
GWLKKWSSGGWTTASRKPVENQDLWKKILQLIKLHNVTFHKVKGHSDNEYNNRCDYLARQAIKNNR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Escherichia coli, GI1786408, Length=144, Percent_Identity=48.6111111111111, Blast_Score=138, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI115532542, Length=140, Percent_Identity=36.4285714285714, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45552509, Length=146, Percent_Identity=30.8219178082192, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137476, Length=146, Percent_Identity=30.8219178082192, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_PROA2 (B4S5K2)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002015246.1
- ProteinModelPortal:   B4S5K2
- GeneID:   6460261
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_0543
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   PRK00203
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 16491; Mature: 16491

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKIIIYTDGACSGNPGKGGWGALLMFGELNREISGYSPATTNNRMELMAAIQALEALK
CCCEEEEEECCCCCCCCCCCCHHHEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EPCDVDLYSDSSYLVNAIKLGWLKKWSSGGWTTASRKPVENQDLWKKILQLIKLHNVTFH
CCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
KVKGHSDNEYNNRCDYLARQAIKNNR
EECCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKKIIIYTDGACSGNPGKGGWGALLMFGELNREISGYSPATTNNRMELMAAIQALEALK
CCCEEEEEECCCCCCCCCCCCHHHEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EPCDVDLYSDSSYLVNAIKLGWLKKWSSGGWTTASRKPVENQDLWKKILQLIKLHNVTFH
CCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
KVKGHSDNEYNNRCDYLARQAIKNNR
EECCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA