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The map label for this gene is queF

Identifier: 194333357

GI number: 194333357

Start: 558596

End: 558952

Strand: Reverse

Name: queF

Synonym: Paes_0514

Alternate gene names: 194333357

Gene position: 558952-558596 (Counterclockwise)

Preceding gene: 194333367

Following gene: 194333355

Centisome position: 22.24

GC content: 47.06

Gene sequence:

>357_bases
ATGAAAAAAGAACTGCTCGAAGTATTTGACAACCAGTTCCCTGACAGGGACTACACTATAGAAATTGTCAATCCCGAATT
CACCTCCGTCTGCCCTAAAACCGGGTTACCTGATTTCGGCACCATTACGCTGCGCTACGTTCCCGACAAGGTGTGTATCG
AACTCAAGTCACTCAAATACTATTACCTGGAATTCCGGAATGCCGGCATCTTTTATGAAAATATCACCAATACCATTCTT
GACCATATGATCAGCGCCCTTCATCCCCGCACGCTTACAGTCACAACAGAATGGAAAGCCCGTGGAGGAATCACCGAAAC
GGTAACGGCATCATACTCCTCTGCCGACAAAGCCTGA

Upstream 100 bases:

>100_bases
AATTCCTGTGAATTCGGATCAGAATTTAAAGAAAATCGCTCAAGATTTCTATTTTTGCTCTGAAAAGGATCAATCTTATG
GCCACTACAACATCATACCC

Downstream 100 bases:

>100_bases
AAAAAAGCCGGATCCTCAACGATAGAAAGCGGCTGATGAGCTCAATGCATCAGCCGCTTTCTATCGTCCTTATCGCACTA
TTCGTCAAAACAAAAAGCCC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 118; Mature: 118

Protein sequence:

>118_residues
MKKELLEVFDNQFPDRDYTIEIVNPEFTSVCPKTGLPDFGTITLRYVPDKVCIELKSLKYYYLEFRNAGIFYENITNTIL
DHMISALHPRTLTVTTEWKARGGITETVTASYSSADKA

Sequences:

>Translated_118_residues
MKKELLEVFDNQFPDRDYTIEIVNPEFTSVCPKTGLPDFGTITLRYVPDKVCIELKSLKYYYLEFRNAGIFYENITNTIL
DHMISALHPRTLTVTTEWKARGGITETVTASYSSADKA
>Mature_118_residues
MKKELLEVFDNQFPDRDYTIEIVNPEFTSVCPKTGLPDFGTITLRYVPDKVCIELKSLKYYYLEFRNAGIFYENITNTIL
DHMISALHPRTLTVTTEWKARGGITETVTASYSSADKA

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_PROA2 (B4S5H3)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002015217.1
- ProteinModelPortal:   B4S5H3
- SMR:   B4S5H3
- GeneID:   6459535
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_0514
- HOGENOM:   HBG294103
- OMA:   FRNAGIF
- ProtClustDB:   PRK13258
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 13522; Mature: 13522

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKELLEVFDNQFPDRDYTIEIVNPEFTSVCPKTGLPDFGTITLRYVPDKVCIELKSLKY
CCHHHHHHHHCCCCCCCEEEEEECCCHHHCCCCCCCCCCCEEEEEECCHHHEEEHHCCEE
YYLEFRNAGIFYENITNTILDHMISALHPRTLTVTTEWKARGGITETVTASYSSADKA
EEEEECCCCEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MKKELLEVFDNQFPDRDYTIEIVNPEFTSVCPKTGLPDFGTITLRYVPDKVCIELKSLKY
CCHHHHHHHHCCCCCCCEEEEEECCCHHHCCCCCCCCCCCEEEEEECCHHHEEEHHCCEE
YYLEFRNAGIFYENITNTILDHMISALHPRTLTVTTEWKARGGITETVTASYSSADKA
EEEEECCCCEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA