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The map label for this gene is hisI

Identifier: 194333274

GI number: 194333274

Start: 463924

End: 464337

Strand: Reverse

Name: hisI

Synonym: Paes_0430

Alternate gene names: 194333274

Gene position: 464337-463924 (Counterclockwise)

Preceding gene: 194333280

Following gene: 194333273

Centisome position: 18.48

GC content: 47.1

Gene sequence:

>414_bases
ATGAGCGAGAATCAAGATCTTCAGAAAAGCTTTCTTGAAACCGTCAAATTCGATGACAAAGGACTTGTTCCCGCCATCGT
TCAGGATTTTGAAACCGGTAAGGTACTCATGATGGCCTGGATGAACCGTCAGAGCCTTGAGATGACCCTGGAAAAAAAGA
AAGCCTGCTACTGGAGCAGAAGCCGTCAGAAACTCTGGCTGAAAGGCGAATCTTCCGGCAATATGCAGAATGTTCACGAT
ATCCTGATCGACTGCGACGGCGATACGCTGCTTCTGAAAGTTTCCCAGACAGGCGGAGCATGCCATATGGGCTATCAATC
CTGTTTTTACCGCAAGACCAAAGATGATCTCTCCATGGAGATCTGTGATACCCTGATGTTTAACCCTGAAGACGTGTATG
GCAAACAAAGCTGA

Upstream 100 bases:

>100_bases
GAAGTTAACAGTTTCAAACTTTTTCTCTTCGCTGACCTTGGTTTTTCCAGAATTTTATCAATCTTTAAGATCTCTTGTAA
CACATTAATCTACCCTATCA

Downstream 100 bases:

>100_bases
AACATCGCCAAACCGGCCCCAGGAAACGGCAAAATCGCTTTTCCGGACGAAGTCACACGCATCGATCCAGCATATGTTCG
ATGAGGTTGCTCCTACCTAC

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: PRA-CH

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MSENQDLQKSFLETVKFDDKGLVPAIVQDFETGKVLMMAWMNRQSLEMTLEKKKACYWSRSRQKLWLKGESSGNMQNVHD
ILIDCDGDTLLLKVSQTGGACHMGYQSCFYRKTKDDLSMEICDTLMFNPEDVYGKQS

Sequences:

>Translated_137_residues
MSENQDLQKSFLETVKFDDKGLVPAIVQDFETGKVLMMAWMNRQSLEMTLEKKKACYWSRSRQKLWLKGESSGNMQNVHD
ILIDCDGDTLLLKVSQTGGACHMGYQSCFYRKTKDDLSMEICDTLMFNPEDVYGKQS
>Mature_136_residues
SENQDLQKSFLETVKFDDKGLVPAIVQDFETGKVLMMAWMNRQSLEMTLEKKKACYWSRSRQKLWLKGESSGNMQNVHDI
LIDCDGDTLLLKVSQTGGACHMGYQSCFYRKTKDDLSMEICDTLMFNPEDVYGKQS

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=89, Percent_Identity=50.561797752809, Blast_Score=100, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319819, Length=105, Percent_Identity=36.1904761904762, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS3_PROA2 (B4S4Y9)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002015134.1
- ProteinModelPortal:   B4S4Y9
- SMR:   B4S4Y9
- GeneID:   6459827
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_0430
- HOGENOM:   HBG294308
- OMA:   LWLKGES
- ProtClustDB:   PRK00051
- GO:   GO:0005737
- HAMAP:   MF_01021
- InterPro:   IPR002496
- ProDom:   PD002610

Pfam domain/function: PF01502 PRA-CH

EC number: =3.5.4.19

Molecular weight: Translated: 15753; Mature: 15622

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
10.2 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
9.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENQDLQKSFLETVKFDDKGLVPAIVQDFETGKVLMMAWMNRQSLEMTLEKKKACYWSR
CCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
SRQKLWLKGESSGNMQNVHDILIDCDGDTLLLKVSQTGGACHMGYQSCFYRKTKDDLSME
CCCEEEEECCCCCCCCCEEEEEEECCCCEEEEEEECCCCEEECCHHHHHHCCCCCHHHHH
ICDTLMFNPEDVYGKQS
HHHHHHCCCHHCCCCCC
>Mature Secondary Structure 
SENQDLQKSFLETVKFDDKGLVPAIVQDFETGKVLMMAWMNRQSLEMTLEKKKACYWSR
CCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
SRQKLWLKGESSGNMQNVHDILIDCDGDTLLLKVSQTGGACHMGYQSCFYRKTKDDLSME
CCCEEEEECCCCCCCCCEEEEEEECCCCEEEEEEECCCCEEECCHHHHHHCCCCCHHHHH
ICDTLMFNPEDVYGKQS
HHHHHHCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA