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The map label for this gene is gltD [H]

Identifier: 194333280

GI number: 194333280

Start: 469092

End: 470540

Strand: Reverse

Name: gltD [H]

Synonym: Paes_0436

Alternate gene names: 194333280

Gene position: 470540-469092 (Counterclockwise)

Preceding gene: 194333281

Following gene: 194333274

Centisome position: 18.72

GC content: 52.66

Gene sequence:

>1449_bases
ATGGACAATAAAACCATTACAACCAAGCAGCGACTGGCCATCCCCCGTCAGGAAATGCCTGCTCAGGATCCATTGAAGCG
CACGCAGAACTTTAAGGAAGTCAACCTCGGCTATAGTCCGGAGGCTGCGATCAAAGAAGCAATGCGCTGCATTCAATGCA
AGGATCCTGTGTGCATTAAAGGCTGTCCGGTCAATATCAAAATCGATGAATTTATTCACCTGGTCGCCGAAGGCGACTTC
CTCGGCGCCGCGAGAAAAATCAAGGAGGATAATGTCCTGCCTGCGATTTGCGGAAGGGTCTGCCCCCAGGAAGACCAGTG
TGAAAAAGAGTGCGTTCTGGGCAATAAGCACAAACCTGTCGCCATCGGTCACCTCGAACGATTTGTCGCCGATTACGAGC
GGGAAAGCGGCAACATCAAAACCCCGGAAGTCCGCCCCGCGACAGGCAAAAAGATAGCCGTCATCGGCAGTGGTCCTGCA
GGACTGAGCTGCGCTAACGACCTTGCTCAGCTCGGCCACAGCGTTGTCGTCTTTGAAGCTCTGCATGAACTCGGAGGAGT
CCTTATGTACGGGGTTCCTGAATTCAGGCTGCCTAAAGAGATTGTAAGAACCGAGATCGACGTTCTGAAAAAAATGGGTG
TAGAATTCCAGTCAGACGTTGTCGTGGGACGCTCGGTCACCATCGATGAACTGATCGAGGAAGAGGGATTCGATGCCGCA
TTCATAGGCGTTGGAGCTGGTCTTCCGTGGTTCATGGGAATACCCGGGGAAAACCTGGTCGGCGTGCTGTCGGCAAACGA
GTTCCTGACAAGGGTCAACCTCATGAAAGCTTATGACTTCCCTGAAAGCGACACTCCGGTCTTTGACTGCAAGGGCAAAA
GCATTGCCGTGTTCGGCGGAGGCAATACCGCAATGGATGCCGTAAGAACGGCAAAACGCCTGGGCGCTGAACACGCCTAC
ATTGTTTACCGGCGTTCGGAAGCAGAAATGCCTGCCAGGGTCGAAGAGATTCACCATGCAAAGGAAGAGGGTATCGAGTT
CCTTCTGCTCCAGAGCCCTGTAGAGTTTATCGGGAATGACGAACACTGGCTGACAGGCGTCAAATGCCTCAAAATGGAAC
TTGGAGAACCCGATGATTCAGGAAGAAGACGGCCTGTGGCTATTGAAGGTTCAGAGTATGTCCTGCCTATAGATATGGCT
GTCATTTCGATCGGCAACGGCTCAAACCCGCTGATCGGGCAGACGACGCCTGATATTGAGTTCAGCAAACGCGACACCAT
TGTTGTCGACATCAACACCATGGGAACATCGAAAGAGAACGTCTACGCAGGCGGAGATATCGTTACCGGCGGCGCAACGG
TTATTCTTGCGATGGGCGCAGGAAGAACAGCTGCCAAAGCCATTGATGAAAAACTCATGGGCAAATCCCGCGGGTTCGAC
GAATGGTAA

Upstream 100 bases:

>100_bases
CAAGCAGATATAATTATACTCTTCAATACATTCAATCCCGACCTTCAAGGTTCTGAAAGGGCCCTTGGGCGGGGATTGAC
AAAAATAACAAGGGTTAACT

Downstream 100 bases:

>100_bases
AACAGGCTGACGCTCCTACTCTAACGGCTGTCCAGAGGAGATCATAACCTCTGGACATTCGTTCCTCCAATCAATCCTCT
TAAGGGGCAACCTTGCCGCT

Product: putative oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MDNKTITTKQRLAIPRQEMPAQDPLKRTQNFKEVNLGYSPEAAIKEAMRCIQCKDPVCIKGCPVNIKIDEFIHLVAEGDF
LGAARKIKEDNVLPAICGRVCPQEDQCEKECVLGNKHKPVAIGHLERFVADYERESGNIKTPEVRPATGKKIAVIGSGPA
GLSCANDLAQLGHSVVVFEALHELGGVLMYGVPEFRLPKEIVRTEIDVLKKMGVEFQSDVVVGRSVTIDELIEEEGFDAA
FIGVGAGLPWFMGIPGENLVGVLSANEFLTRVNLMKAYDFPESDTPVFDCKGKSIAVFGGGNTAMDAVRTAKRLGAEHAY
IVYRRSEAEMPARVEEIHHAKEEGIEFLLLQSPVEFIGNDEHWLTGVKCLKMELGEPDDSGRRRPVAIEGSEYVLPIDMA
VISIGNGSNPLIGQTTPDIEFSKRDTIVVDINTMGTSKENVYAGGDIVTGGATVILAMGAGRTAAKAIDEKLMGKSRGFD
EW

Sequences:

>Translated_482_residues
MDNKTITTKQRLAIPRQEMPAQDPLKRTQNFKEVNLGYSPEAAIKEAMRCIQCKDPVCIKGCPVNIKIDEFIHLVAEGDF
LGAARKIKEDNVLPAICGRVCPQEDQCEKECVLGNKHKPVAIGHLERFVADYERESGNIKTPEVRPATGKKIAVIGSGPA
GLSCANDLAQLGHSVVVFEALHELGGVLMYGVPEFRLPKEIVRTEIDVLKKMGVEFQSDVVVGRSVTIDELIEEEGFDAA
FIGVGAGLPWFMGIPGENLVGVLSANEFLTRVNLMKAYDFPESDTPVFDCKGKSIAVFGGGNTAMDAVRTAKRLGAEHAY
IVYRRSEAEMPARVEEIHHAKEEGIEFLLLQSPVEFIGNDEHWLTGVKCLKMELGEPDDSGRRRPVAIEGSEYVLPIDMA
VISIGNGSNPLIGQTTPDIEFSKRDTIVVDINTMGTSKENVYAGGDIVTGGATVILAMGAGRTAAKAIDEKLMGKSRGFD
EW
>Mature_482_residues
MDNKTITTKQRLAIPRQEMPAQDPLKRTQNFKEVNLGYSPEAAIKEAMRCIQCKDPVCIKGCPVNIKIDEFIHLVAEGDF
LGAARKIKEDNVLPAICGRVCPQEDQCEKECVLGNKHKPVAIGHLERFVADYERESGNIKTPEVRPATGKKIAVIGSGPA
GLSCANDLAQLGHSVVVFEALHELGGVLMYGVPEFRLPKEIVRTEIDVLKKMGVEFQSDVVVGRSVTIDELIEEEGFDAA
FIGVGAGLPWFMGIPGENLVGVLSANEFLTRVNLMKAYDFPESDTPVFDCKGKSIAVFGGGNTAMDAVRTAKRLGAEHAY
IVYRRSEAEMPARVEEIHHAKEEGIEFLLLQSPVEFIGNDEHWLTGVKCLKMELGEPDDSGRRRPVAIEGSEYVLPIDMA
VISIGNGSNPLIGQTTPDIEFSKRDTIVVDINTMGTSKENVYAGGDIVTGGATVILAMGAGRTAAKAIDEKLMGKSRGFD
EW

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=467, Percent_Identity=32.3340471092077, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI237757300, Length=103, Percent_Identity=39.8058252427184, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1789606, Length=454, Percent_Identity=37.8854625550661, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1788811, Length=458, Percent_Identity=36.6812227074236, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI87082180, Length=448, Percent_Identity=37.0535714285714, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1788468, Length=460, Percent_Identity=31.5217391304348, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1789243, Length=462, Percent_Identity=31.8181818181818, Blast_Score=168, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17570289, Length=487, Percent_Identity=28.7474332648871, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI71984108, Length=456, Percent_Identity=30.0438596491228, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17543860, Length=170, Percent_Identity=31.7647058823529, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=482, Percent_Identity=30.7053941908714, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI28574881, Length=500, Percent_Identity=29.8, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24665539, Length=500, Percent_Identity=29.8, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24665547, Length=500, Percent_Identity=29.8, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24665543, Length=500, Percent_Identity=29.8, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24640763, Length=452, Percent_Identity=31.6371681415929, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI18858217, Length=452, Percent_Identity=31.6371681415929, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52485; Mature: 52485

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNKTITTKQRLAIPRQEMPAQDPLKRTQNFKEVNLGYSPEAAIKEAMRCIQCKDPVCIK
CCCCEECCHHHHCCCHHHCCCCCHHHHHCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEE
GCPVNIKIDEFIHLVAEGDFLGAARKIKEDNVLPAICGRVCPQEDQCEKECVLGNKHKPV
CCCEEEEHHHHEEEHCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHCCCCCCCE
AIGHLERFVADYERESGNIKTPEVRPATGKKIAVIGSGPAGLSCANDLAQLGHSVVVFEA
EHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHH
LHELGGVLMYGVPEFRLPKEIVRTEIDVLKKMGVEFQSDVVVGRSVTIDELIEEEGFDAA
HHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHCCCCEE
FIGVGAGLPWFMGIPGENLVGVLSANEFLTRVNLMKAYDFPESDTPVFDCKGKSIAVFGG
EEECCCCCCEEECCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEC
GNTAMDAVRTAKRLGAEHAYIVYRRSEAEMPARVEEIHHAKEEGIEFLLLQSPVEFIGND
CCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHCCC
EHWLTGVKCLKMELGEPDDSGRRRPVAIEGSEYVLPIDMAVISIGNGSNPLIGQTTPDIE
CHHHHHHHEEEEECCCCCCCCCCCCEEECCCCEEEEEEEEEEEECCCCCCCCCCCCCCCE
FSKRDTIVVDINTMGTSKENVYAGGDIVTGGATVILAMGAGRTAAKAIDEKLMGKSRGFD
ECCCCEEEEEEECCCCCCCCEEECCCEEECCEEEEEEECCCCHHHHHHHHHHHHCCCCCC
EW
CC
>Mature Secondary Structure
MDNKTITTKQRLAIPRQEMPAQDPLKRTQNFKEVNLGYSPEAAIKEAMRCIQCKDPVCIK
CCCCEECCHHHHCCCHHHCCCCCHHHHHCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEE
GCPVNIKIDEFIHLVAEGDFLGAARKIKEDNVLPAICGRVCPQEDQCEKECVLGNKHKPV
CCCEEEEHHHHEEEHCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHCCCCCCCE
AIGHLERFVADYERESGNIKTPEVRPATGKKIAVIGSGPAGLSCANDLAQLGHSVVVFEA
EHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHH
LHELGGVLMYGVPEFRLPKEIVRTEIDVLKKMGVEFQSDVVVGRSVTIDELIEEEGFDAA
HHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHCCCCEE
FIGVGAGLPWFMGIPGENLVGVLSANEFLTRVNLMKAYDFPESDTPVFDCKGKSIAVFGG
EEECCCCCCEEECCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEC
GNTAMDAVRTAKRLGAEHAYIVYRRSEAEMPARVEEIHHAKEEGIEFLLLQSPVEFIGND
CCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHCCC
EHWLTGVKCLKMELGEPDDSGRRRPVAIEGSEYVLPIDMAVISIGNGSNPLIGQTTPDIE
CHHHHHHHEEEEECCCCCCCCCCCCEEECCCCEEEEEEEEEEEECCCCCCCCCCCCCCCE
FSKRDTIVVDINTMGTSKENVYAGGDIVTGGATVILAMGAGRTAAKAIDEKLMGKSRGFD
ECCCCEEEEEEECCCCCCCCEEECCCEEECCEEEEEEECCCCHHHHHHHHHHHHCCCCCC
EW
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]