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The map label for this gene is dapF [H]

Identifier: 194333117

GI number: 194333117

Start: 285752

End: 286546

Strand: Reverse

Name: dapF [H]

Synonym: Paes_0273

Alternate gene names: 194333117

Gene position: 286546-285752 (Counterclockwise)

Preceding gene: 194333121

Following gene: 194333116

Centisome position: 11.4

GC content: 51.57

Gene sequence:

>795_bases
ATGAACACATCGATCAAGTTTTCTAAAATGTCGGGTGCCGGGAACGATTTCATCGTTTTCGATAACATGCACAAGGACAT
TCATCTGTCTGCCGACAACATCCGCTCTCTCTGCACGAGGCGCACAGGTATCGGCGCTGACGGTCTTATTCTCATCGAAC
CGTCAGCTCGGTTCGACTTTGCCATGAAGTACTATAATGCCGACGGGCTGCCAGGATCGATGTGCGGAAACGGCGGTCGG
TGTGCTGTGTATTTCGCATGGACCATCGGCGCCTGCGGGACAACAACGGCATTTGAAGCTAACGGAAACCGTTATGATGC
GTGGGTTTGTGACAATGAAGCGATCAGGCTCAAAATGATTCTCCCCCACGACTTCCGCGACGAGTTTGGCTCGAACGGCT
TGAGCTGCAACTTTGTCAATACGGGATCACCGCATACCATTATCTTCACCGATGAGCTCGACACCTTTGACGTTGAATCA
ACAGGGCGTCTGATACGTAACGACAGCAAGCTCTTTCCTGAAGGAACAAACGTCAATTTTCTGCAGGTCACCTCTCCTGA
AAGCCTCAAGGTAAGAACGTATGAGCGGGGGGTCGAAGCAGAAACGCTTGCATGCGGCACCGGGGCCGTAGCCGCTGCGT
TAATGAGTTACAAGCTCGGAAAGATTGCCTCGAGAAGCGTTTCGGTCAGCGTCAGAAGCGGAGATATTCTGACCATCGAT
TTTTCAGAAGACATGCAGGATGTCTATCTCTCCGGACCGGCGACCATTGTGTATACCGGGTCAATACAGATGTGA

Upstream 100 bases:

>100_bases
TTGGGGCTTCCCGGCCTTGCGCCGTCGAACGGCATCTGTTACTATAACTGAAGATACAGGCCCGCAGAATCCCCAGCTCA
ACGACACAATATATTGATTC

Downstream 100 bases:

>100_bases
TATCGAGTGGCTCCAAAACGGCAGGGGGACACATCACTGAAGCCAAAGCAGTATTCCGGCATTGCCCGCTGTACTAACCG
GCACCGTGTGATCGTTGGTC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNTSIKFSKMSGAGNDFIVFDNMHKDIHLSADNIRSLCTRRTGIGADGLILIEPSARFDFAMKYYNADGLPGSMCGNGGR
CAVYFAWTIGACGTTTAFEANGNRYDAWVCDNEAIRLKMILPHDFRDEFGSNGLSCNFVNTGSPHTIIFTDELDTFDVES
TGRLIRNDSKLFPEGTNVNFLQVTSPESLKVRTYERGVEAETLACGTGAVAAALMSYKLGKIASRSVSVSVRSGDILTID
FSEDMQDVYLSGPATIVYTGSIQM

Sequences:

>Translated_264_residues
MNTSIKFSKMSGAGNDFIVFDNMHKDIHLSADNIRSLCTRRTGIGADGLILIEPSARFDFAMKYYNADGLPGSMCGNGGR
CAVYFAWTIGACGTTTAFEANGNRYDAWVCDNEAIRLKMILPHDFRDEFGSNGLSCNFVNTGSPHTIIFTDELDTFDVES
TGRLIRNDSKLFPEGTNVNFLQVTSPESLKVRTYERGVEAETLACGTGAVAAALMSYKLGKIASRSVSVSVRSGDILTID
FSEDMQDVYLSGPATIVYTGSIQM
>Mature_264_residues
MNTSIKFSKMSGAGNDFIVFDNMHKDIHLSADNIRSLCTRRTGIGADGLILIEPSARFDFAMKYYNADGLPGSMCGNGGR
CAVYFAWTIGACGTTTAFEANGNRYDAWVCDNEAIRLKMILPHDFRDEFGSNGLSCNFVNTGSPHTIIFTDELDTFDVES
TGRLIRNDSKLFPEGTNVNFLQVTSPESLKVRTYERGVEAETLACGTGAVAAALMSYKLGKIASRSVSVSVRSGDILTID
FSEDMQDVYLSGPATIVYTGSIQM

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=278, Percent_Identity=33.8129496402878, Blast_Score=148, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 28712; Mature: 28712

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSIKFSKMSGAGNDFIVFDNMHKDIHLSADNIRSLCTRRTGIGADGLILIEPSARFDF
CCCCEEEEECCCCCCEEEEEECCCCEEEECHHHHHHHHHHCCCCCCCCEEEECCCCCCEE
AMKYYNADGLPGSMCGNGGRCAVYFAWTIGACGTTTAFEANGNRYDAWVCDNEAIRLKMI
EEEEECCCCCCHHHCCCCCEEEEEEEEEECCCCCCEEEECCCCEEEEEEECCCCEEEEEE
LPHDFRDEFGSNGLSCNFVNTGSPHTIIFTDELDTFDVESTGRLIRNDSKLFPEGTNVNF
CCCCHHHHHCCCCCEEEEEECCCCEEEEEECCCCCEECCCCCCEEECCCCCCCCCCCEEE
LQVTSPESLKVRTYERGVEAETLACGTGAVAAALMSYKLGKIASRSVSVSVRSGDILTID
EEECCCCCEEEEEECCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEE
FSEDMQDVYLSGPATIVYTGSIQM
CCCCCCEEEECCCEEEEEEEEECC
>Mature Secondary Structure
MNTSIKFSKMSGAGNDFIVFDNMHKDIHLSADNIRSLCTRRTGIGADGLILIEPSARFDF
CCCCEEEEECCCCCCEEEEEECCCCEEEECHHHHHHHHHHCCCCCCCCEEEECCCCCCEE
AMKYYNADGLPGSMCGNGGRCAVYFAWTIGACGTTTAFEANGNRYDAWVCDNEAIRLKMI
EEEEECCCCCCHHHCCCCCEEEEEEEEEECCCCCCEEEECCCCEEEEEEECCCCEEEEEE
LPHDFRDEFGSNGLSCNFVNTGSPHTIIFTDELDTFDVESTGRLIRNDSKLFPEGTNVNF
CCCCHHHHHCCCCCEEEEEECCCCEEEEEECCCCCEECCCCCCEEECCCCCCCCCCCEEE
LQVTSPESLKVRTYERGVEAETLACGTGAVAAALMSYKLGKIASRSVSVSVRSGDILTID
EEECCCCCEEEEEECCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEE
FSEDMQDVYLSGPATIVYTGSIQM
CCCCCCEEEECCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]