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The map label for this gene is fusA [H]

Identifier: 194333121

GI number: 194333121

Start: 290808

End: 292880

Strand: Reverse

Name: fusA [H]

Synonym: Paes_0277

Alternate gene names: 194333121

Gene position: 292880-290808 (Counterclockwise)

Preceding gene: 194333134

Following gene: 194333117

Centisome position: 11.65

GC content: 49.16

Gene sequence:

>2073_bases
ATGCAAGCCGTTCAACCTGATCAACTTCGCAACATCGTCATTACCGGCCATTCAGGTTCAGGTAAAACAATGCTCGCCGA
ATCATTTGCGTTGTCAATGAAGGTCATCAACCGTCTGGGCAGTATTGAGGATGGTTCGACATTATCAGATTACGCGGAAG
ATGAAATTGCGCGACAACACAGTCTGAACACAAGCTTGATCAACGGCGAGTGGAACGGCAACAAAATCAACATCATCGAC
GTACCCGGCCTGCTTGACTTTCACGGTGATGTCAAGTCTGCAATGCGCGTGGCAGATACCATCCTCATTACGGTCAACTC
GGCAACAGGACTTGAAGTCGGAACAGACATGGTATGGGAGTATACACGCGAATACTATAAGCCGACAATATTTGTGCTCA
CCAAACTCGATGCAGACAGAACAGACTTCGAGCAGACTATAGAGGATCTGAAAAAACAGTACGGTCATCTTGTTACCCCT
ATACAATTTCCGGCTGAAGAAGGTCTCGGTCATCATATCATCATCGACGTCCTCCTGATGAAACAGCTTGAATTCAGTCC
TGACAAACCCGGGACCATGACGATCAGTGAGATTCCTGACCTTTACCGCAAAAAAGCTGAAGAGCTTCATCACAACCTTG
TCGAAGCGGTCGCAGAAACCGATGAGGAACTGATGAACAAGTATTTTGAAATTGGAACCCTCAGCGAAGATGAATTGCGT
GCCGGCATAAAGTCGTCCCTCCTGACAAGAACCTTTTTCCCTGTTTTCTGTACCTCCCCGCTCCACCTTATAGGATCCGA
ACGTCTGCTCAACGCCATTGTCAACATTTGCCCGTCCCCTATCGAACGCGGCCCTGAACATGCATACTGCAGCACTGAAA
ACCATGATACGCTGATCGAACCCGATCCGGAAGGCAGAACGATAGCATTTATATTCAAAACGCTTTCAGAACCGCGCCTT
GGCGAAATCTCCTATCTCAGAGTCTACTCGGGCCATATTGAAACAGGACATGAGCTTATTGACGCACAGACAGGACAGCT
TGAGAAAATCGGCCAGGTCTATACCATGGCCGGTGAGAAAAAAATTCCTGTAGACAAGCTGGTTGCCGGAGACATCGGCA
TGGTTGTCAAGCTGAAGGATGCCCACACGAATGACACCCTGGCAGACAAAGGCACGGACTGCCGGATCAGTCCGATCATC
TTTCCTGAACCGGTCTATGCAACCGCAATCGCACCAGTAGCTCAGGGGGATGAGGAAAAAATTTCCAGCGGTCTGCATCA
TCTTCATGAGGAAGACCCGAGCTTCAGTATCGAGCATGAACCGGAGTTCAATCAGACGATCCTGAAAACTCTCGGCGAAA
CTCATCTGGACATTATCATCAACAGGCTCGAAAAAAAGTTTAACGTATCCGTGCAACGGCTTCCGGTAAAAATATCCTAC
AGGGAAACCATAAGGGTCAGCGCAAATGCACAGGGAAAATACAAAAAACAATCAGGGGGCCGTGGACAGTATGGCGACGT
ATGGATCCGAATTGATCCGGCAGAGCGTGAGACAGGATTTGCGTTTGCAAGTGAAGTTGTCGGCGGAGTCGTCCCGACCC
GATACCTCCCTGCTGTAGAAAAGGGCCTTCGCGAAACAGCGTCTGAAGGAATACTCTGCGGATTCCCGATTGTCGATATC
AAAGCAGTCGTCTACGATGGCTCCTATCACCCGGTTGACAGCTCTGAATTCGCCTTCAAGATCGCATCAAGCATGGCATT
TAAATCAGCGTTCGACAAAGCCAAGCCGATGCTGCTCGAGCCCTACTGCACGCTTCGCGTTCTTGCTCCTGAACAGTATA
CCGGTGAAATCGTCGGAGAAATATCGGTGAAGCGCGGTAAAATTGTCGGGATGGACGCCGATGGGAGATTTCAGACAATC
AATGCGCTGATTCCCCAGGCATCACTGCACGATTTCCATACCCGTCTGGTAAGACTGACACAAAGCAGAGCTCGCTACAG
TTATGCATTCAGCCACTACGAGGAAGTGCCGCAGGAAATAATGCAGCAGCTCATGAAGGAATACGAAAAATAA

Upstream 100 bases:

>100_bases
TACTATTCTGCTGTTTCACACAGACACGTCAAACAGCTCTAAGCCATTGTCTGGAAAGGCCCTCCGGGCCATCAGCAATC
AAAATAAAGGAGTACAAACC

Downstream 100 bases:

>100_bases
TCAGCGTGAATCTGAGTGTGCAACTCCCTGCCTGTAGCCTGCAGAGAATGTTCTGAAAACATTCCTGCAGGCTACAGGAG
TCGCAGGATGGATTTCGCCT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 690; Mature: 690

Protein sequence:

>690_residues
MQAVQPDQLRNIVITGHSGSGKTMLAESFALSMKVINRLGSIEDGSTLSDYAEDEIARQHSLNTSLINGEWNGNKINIID
VPGLLDFHGDVKSAMRVADTILITVNSATGLEVGTDMVWEYTREYYKPTIFVLTKLDADRTDFEQTIEDLKKQYGHLVTP
IQFPAEEGLGHHIIIDVLLMKQLEFSPDKPGTMTISEIPDLYRKKAEELHHNLVEAVAETDEELMNKYFEIGTLSEDELR
AGIKSSLLTRTFFPVFCTSPLHLIGSERLLNAIVNICPSPIERGPEHAYCSTENHDTLIEPDPEGRTIAFIFKTLSEPRL
GEISYLRVYSGHIETGHELIDAQTGQLEKIGQVYTMAGEKKIPVDKLVAGDIGMVVKLKDAHTNDTLADKGTDCRISPII
FPEPVYATAIAPVAQGDEEKISSGLHHLHEEDPSFSIEHEPEFNQTILKTLGETHLDIIINRLEKKFNVSVQRLPVKISY
RETIRVSANAQGKYKKQSGGRGQYGDVWIRIDPAERETGFAFASEVVGGVVPTRYLPAVEKGLRETASEGILCGFPIVDI
KAVVYDGSYHPVDSSEFAFKIASSMAFKSAFDKAKPMLLEPYCTLRVLAPEQYTGEIVGEISVKRGKIVGMDADGRFQTI
NALIPQASLHDFHTRLVRLTQSRARYSYAFSHYEEVPQEIMQQLMKEYEK

Sequences:

>Translated_690_residues
MQAVQPDQLRNIVITGHSGSGKTMLAESFALSMKVINRLGSIEDGSTLSDYAEDEIARQHSLNTSLINGEWNGNKINIID
VPGLLDFHGDVKSAMRVADTILITVNSATGLEVGTDMVWEYTREYYKPTIFVLTKLDADRTDFEQTIEDLKKQYGHLVTP
IQFPAEEGLGHHIIIDVLLMKQLEFSPDKPGTMTISEIPDLYRKKAEELHHNLVEAVAETDEELMNKYFEIGTLSEDELR
AGIKSSLLTRTFFPVFCTSPLHLIGSERLLNAIVNICPSPIERGPEHAYCSTENHDTLIEPDPEGRTIAFIFKTLSEPRL
GEISYLRVYSGHIETGHELIDAQTGQLEKIGQVYTMAGEKKIPVDKLVAGDIGMVVKLKDAHTNDTLADKGTDCRISPII
FPEPVYATAIAPVAQGDEEKISSGLHHLHEEDPSFSIEHEPEFNQTILKTLGETHLDIIINRLEKKFNVSVQRLPVKISY
RETIRVSANAQGKYKKQSGGRGQYGDVWIRIDPAERETGFAFASEVVGGVVPTRYLPAVEKGLRETASEGILCGFPIVDI
KAVVYDGSYHPVDSSEFAFKIASSMAFKSAFDKAKPMLLEPYCTLRVLAPEQYTGEIVGEISVKRGKIVGMDADGRFQTI
NALIPQASLHDFHTRLVRLTQSRARYSYAFSHYEEVPQEIMQQLMKEYEK
>Mature_690_residues
MQAVQPDQLRNIVITGHSGSGKTMLAESFALSMKVINRLGSIEDGSTLSDYAEDEIARQHSLNTSLINGEWNGNKINIID
VPGLLDFHGDVKSAMRVADTILITVNSATGLEVGTDMVWEYTREYYKPTIFVLTKLDADRTDFEQTIEDLKKQYGHLVTP
IQFPAEEGLGHHIIIDVLLMKQLEFSPDKPGTMTISEIPDLYRKKAEELHHNLVEAVAETDEELMNKYFEIGTLSEDELR
AGIKSSLLTRTFFPVFCTSPLHLIGSERLLNAIVNICPSPIERGPEHAYCSTENHDTLIEPDPEGRTIAFIFKTLSEPRL
GEISYLRVYSGHIETGHELIDAQTGQLEKIGQVYTMAGEKKIPVDKLVAGDIGMVVKLKDAHTNDTLADKGTDCRISPII
FPEPVYATAIAPVAQGDEEKISSGLHHLHEEDPSFSIEHEPEFNQTILKTLGETHLDIIINRLEKKFNVSVQRLPVKISY
RETIRVSANAQGKYKKQSGGRGQYGDVWIRIDPAERETGFAFASEVVGGVVPTRYLPAVEKGLRETASEGILCGFPIVDI
KAVVYDGSYHPVDSSEFAFKIASSMAFKSAFDKAKPMLLEPYCTLRVLAPEQYTGEIVGEISVKRGKIVGMDADGRFQTI
NALIPQASLHDFHTRLVRLTQSRARYSYAFSHYEEVPQEIMQQLMKEYEK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=703, Percent_Identity=28.3072546230441, Blast_Score=299, Evalue=5e-81,
Organism=Homo sapiens, GI19923640, Length=713, Percent_Identity=24.4039270687237, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI25306287, Length=691, Percent_Identity=24.4573082489146, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI25306283, Length=391, Percent_Identity=25.8312020460358, Blast_Score=133, Evalue=5e-31,
Organism=Escherichia coli, GI1789738, Length=696, Percent_Identity=34.3390804597701, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1790835, Length=481, Percent_Identity=23.2848232848233, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=703, Percent_Identity=26.3157894736842, Blast_Score=280, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17556745, Length=721, Percent_Identity=23.0235783633842, Blast_Score=166, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6323098, Length=692, Percent_Identity=27.8901734104046, Blast_Score=312, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6322359, Length=797, Percent_Identity=23.7139272271016, Blast_Score=219, Evalue=8e-58,
Organism=Drosophila melanogaster, GI24582462, Length=695, Percent_Identity=27.9136690647482, Blast_Score=307, Evalue=2e-83,
Organism=Drosophila melanogaster, GI221458488, Length=724, Percent_Identity=23.2044198895028, Blast_Score=185, Evalue=7e-47,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77032; Mature: 77032

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAVQPDQLRNIVITGHSGSGKTMLAESFALSMKVINRLGSIEDGSTLSDYAEDEIARQH
CCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
SLNTSLINGEWNGNKINIIDVPGLLDFHGDVKSAMRVADTILITVNSATGLEVGTDMVWE
CCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHEEEEEEEECCCCCCCCHHHHHH
YTREYYKPTIFVLTKLDADRTDFEQTIEDLKKQYGHLVTPIQFPAEEGLGHHIIIDVLLM
HHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCEECCEECCHHHCCCHHHHHHHHHH
KQLEFSPDKPGTMTISEIPDLYRKKAEELHHNLVEAVAETDEELMNKYFEIGTLSEDELR
HHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AGIKSSLLTRTFFPVFCTSPLHLIGSERLLNAIVNICPSPIERGPEHAYCSTENHDTLIE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCEECCCCCCCEEC
PDPEGRTIAFIFKTLSEPRLGEISYLRVYSGHIETGHELIDAQTGQLEKIGQVYTMAGEK
CCCCCCEEEEEEHHCCCCCCCCEEEEEEEECCCCCCHHHHCCCCCCHHHHHHHHEECCCC
KIPVDKLVAGDIGMVVKLKDAHTNDTLADKGTDCRISPIIFPEPVYATAIAPVAQGDEEK
CCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEECCEECCCCHHHHHHHHHCCCCHHH
ISSGLHHLHEEDPSFSIEHEPEFNQTILKTLGETHLDIIINRLEKKFNVSVQRLPVKISY
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCEEEEEEEEEEE
RETIRVSANAQGKYKKQSGGRGQYGDVWIRIDPAERETGFAFASEVVGGVVPTRYLPAVE
EEEEEEECCCCCCEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHH
KGLRETASEGILCGFPIVDIKAVVYDGSYHPVDSSEFAFKIASSMAFKSAFDKAKPMLLE
HHHHHHHHCCCEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEC
PYCTLRVLAPEQYTGEIVGEISVKRGKIVGMDADGRFQTINALIPQASLHDFHTRLVRLT
CCEEEEEECCCCCCCCEEEEEEEECCEEEEECCCCCEEEHHHHCCCCHHHHHHHHHHHHH
QSRARYSYAFSHYEEVPQEIMQQLMKEYEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQAVQPDQLRNIVITGHSGSGKTMLAESFALSMKVINRLGSIEDGSTLSDYAEDEIARQH
CCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
SLNTSLINGEWNGNKINIIDVPGLLDFHGDVKSAMRVADTILITVNSATGLEVGTDMVWE
CCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHEEEEEEEECCCCCCCCHHHHHH
YTREYYKPTIFVLTKLDADRTDFEQTIEDLKKQYGHLVTPIQFPAEEGLGHHIIIDVLLM
HHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCEECCEECCHHHCCCHHHHHHHHHH
KQLEFSPDKPGTMTISEIPDLYRKKAEELHHNLVEAVAETDEELMNKYFEIGTLSEDELR
HHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AGIKSSLLTRTFFPVFCTSPLHLIGSERLLNAIVNICPSPIERGPEHAYCSTENHDTLIE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCEECCCCCCCEEC
PDPEGRTIAFIFKTLSEPRLGEISYLRVYSGHIETGHELIDAQTGQLEKIGQVYTMAGEK
CCCCCCEEEEEEHHCCCCCCCCEEEEEEEECCCCCCHHHHCCCCCCHHHHHHHHEECCCC
KIPVDKLVAGDIGMVVKLKDAHTNDTLADKGTDCRISPIIFPEPVYATAIAPVAQGDEEK
CCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEECCEECCCCHHHHHHHHHCCCCHHH
ISSGLHHLHEEDPSFSIEHEPEFNQTILKTLGETHLDIIINRLEKKFNVSVQRLPVKISY
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCEEEEEEEEEEE
RETIRVSANAQGKYKKQSGGRGQYGDVWIRIDPAERETGFAFASEVVGGVVPTRYLPAVE
EEEEEEECCCCCCEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHH
KGLRETASEGILCGFPIVDIKAVVYDGSYHPVDSSEFAFKIASSMAFKSAFDKAKPMLLE
HHHHHHHHCCCEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEC
PYCTLRVLAPEQYTGEIVGEISVKRGKIVGMDADGRFQTINALIPQASLHDFHTRLVRLT
CCEEEEEECCCCCCCCEEEEEEEECCEEEEECCCCCEEEHHHHCCCCHHHHHHHHHHHHH
QSRARYSYAFSHYEEVPQEIMQQLMKEYEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]