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The map label for this gene is hemL [H]

Identifier: 194333039

GI number: 194333039

Start: 198758

End: 200053

Strand: Reverse

Name: hemL [H]

Synonym: Paes_0193

Alternate gene names: 194333039

Gene position: 200053-198758 (Counterclockwise)

Preceding gene: 194333040

Following gene: 194333035

Centisome position: 7.96

GC content: 54.86

Gene sequence:

>1296_bases
ATGCCTCAATTAACCAGATCAGCAGAACTCTTTGAAAAAGCAAAGCAGTTTATTCCAGGCGGCGTAAACTCCCCGGTACG
TGCCTTCAAATCGGTTGGCGGCACCCCTATCTACATGGCAAAGGGCCAGGGCGCCTACATGACCGACGTTGACGGCAATA
CCTATCTGGATTACGTTGGCTCATGGGGACCTTTCATTCTCGGCAGCATGCACCCAAGAATCACTGCCGCACTCGAGCAC
ACCCTGACCAAAATCGGCACCAGTTTCGGCACCCCGATTGAAATGGAAATCGAGATTGCAGAACTGCTCTGCCAGATCGT
TCCGTCGATCGAAATGGTCCGCATGGTCAACAGCGGAACCGAAGCGACCATGTCTGCTGTGCGCCTTGCGCGAGGCTATA
CCAGCCGTGACAAAATCATCAAATTTGAAGGCTGCTACCATGGTCATGGAGACAGCTTCCTGATCAAAGCCGGCTCAGGC
GCTCTGACGCTTGGCGCTCCCGACAGCCCCGGCGTCACCAAAGGCACCGCAAACGATACGCTGAACGCGAAATACAACGA
TATCGAATCTGTCAGACTGCTCGTCAATGAGAACAAAGGCAATATTGCTGCCATTATTATTGAACCGGTTGCAGGCAACA
CCGGCGTGATTCCTGCCAAGCCAGGCTTCCTGCAGGCGCTGCGCGATCTGTGTACCGAAGAGGGTATCGTGCTTATTTTC
GACGAGGTCATGTGCGGCTTCCGCGTAGCGCTCGGCGGCGCTCAGGAACGTTACGGCGTCACCCCGGATCTTACCACCAT
GGGTAAAATCATCGGCGGCGGTCTTCCTGTCGGCGCTTTCGGCGGAAAACGTGAAATCATGGAACGCATTGCTCCGCTCG
GCGATGTCTATCAGGCAGGAACACTTTCCGGCAACCCGCTCGCACTGACAGCTGGTCTGGAAACGCTCAAGATTCTGCGC
GACGAAAACCCTTACCCGGAACTCGAAAGAAAAGCAGCGTTCCTCGAAGAGGGCTTCCGCAACAACATGAACAAGCTCGG
CCTGAACTACGTACAGAACCGCGTCGGATCCATGGCATGTCTCTTCTTCACCGAAACTCCGGTTACCGACTACGATACCG
CGGTCACTGCGGACCTGAAAAAGTACGGCACCTACTACCATGCCATGCTCGATCAGGGCATCTACCTCGCACCATCGCAG
TTTGAAGCGATGTTCACCAGCGCAGTCATGACCGACGAGGATCTTGAGAAAACCGTCAAGGCCAACTACGTCGCACTCCA
GGCTACCCAGAAGTAA

Upstream 100 bases:

>100_bases
AGTGAGTTTCCGGGTTTTATATCTTCGTGAGTATTTCTATCTTGAAGGTTTAATACTCATGCATCATGTCTTCATTTAAT
CTTTAATCGAATTGGTGAAT

Downstream 100 bases:

>100_bases
AACACCGGATATTCCTCAGGGAAACAGAAACATCCGGAGCGGGAAACGTCTTATGGCGTTTCCCGCTTTTTTTATGCCGT
TGGTCGCCCGACGACTGCGG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPQLTRSAELFEKAKQFIPGGVNSPVRAFKSVGGTPIYMAKGQGAYMTDVDGNTYLDYVGSWGPFILGSMHPRITAALEH
TLTKIGTSFGTPIEMEIEIAELLCQIVPSIEMVRMVNSGTEATMSAVRLARGYTSRDKIIKFEGCYHGHGDSFLIKAGSG
ALTLGAPDSPGVTKGTANDTLNAKYNDIESVRLLVNENKGNIAAIIIEPVAGNTGVIPAKPGFLQALRDLCTEEGIVLIF
DEVMCGFRVALGGAQERYGVTPDLTTMGKIIGGGLPVGAFGGKREIMERIAPLGDVYQAGTLSGNPLALTAGLETLKILR
DENPYPELERKAAFLEEGFRNNMNKLGLNYVQNRVGSMACLFFTETPVTDYDTAVTADLKKYGTYYHAMLDQGIYLAPSQ
FEAMFTSAVMTDEDLEKTVKANYVALQATQK

Sequences:

>Translated_431_residues
MPQLTRSAELFEKAKQFIPGGVNSPVRAFKSVGGTPIYMAKGQGAYMTDVDGNTYLDYVGSWGPFILGSMHPRITAALEH
TLTKIGTSFGTPIEMEIEIAELLCQIVPSIEMVRMVNSGTEATMSAVRLARGYTSRDKIIKFEGCYHGHGDSFLIKAGSG
ALTLGAPDSPGVTKGTANDTLNAKYNDIESVRLLVNENKGNIAAIIIEPVAGNTGVIPAKPGFLQALRDLCTEEGIVLIF
DEVMCGFRVALGGAQERYGVTPDLTTMGKIIGGGLPVGAFGGKREIMERIAPLGDVYQAGTLSGNPLALTAGLETLKILR
DENPYPELERKAAFLEEGFRNNMNKLGLNYVQNRVGSMACLFFTETPVTDYDTAVTADLKKYGTYYHAMLDQGIYLAPSQ
FEAMFTSAVMTDEDLEKTVKANYVALQATQK
>Mature_430_residues
PQLTRSAELFEKAKQFIPGGVNSPVRAFKSVGGTPIYMAKGQGAYMTDVDGNTYLDYVGSWGPFILGSMHPRITAALEHT
LTKIGTSFGTPIEMEIEIAELLCQIVPSIEMVRMVNSGTEATMSAVRLARGYTSRDKIIKFEGCYHGHGDSFLIKAGSGA
LTLGAPDSPGVTKGTANDTLNAKYNDIESVRLLVNENKGNIAAIIIEPVAGNTGVIPAKPGFLQALRDLCTEEGIVLIFD
EVMCGFRVALGGAQERYGVTPDLTTMGKIIGGGLPVGAFGGKREIMERIAPLGDVYQAGTLSGNPLALTAGLETLKILRD
ENPYPELERKAAFLEEGFRNNMNKLGLNYVQNRVGSMACLFFTETPVTDYDTAVTADLKKYGTYYHAMLDQGIYLAPSQF
EAMFTSAVMTDEDLEKTVKANYVALQATQK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=325, Percent_Identity=30.4615384615385, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI13994255, Length=324, Percent_Identity=29.320987654321, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI37574042, Length=331, Percent_Identity=28.0966767371601, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI226442705, Length=331, Percent_Identity=28.0966767371601, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI24119277, Length=305, Percent_Identity=26.5573770491803, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI226442709, Length=300, Percent_Identity=27.6666666666667, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI284507298, Length=242, Percent_Identity=31.8181818181818, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1786349, Length=419, Percent_Identity=54.4152744630072, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1789016, Length=331, Percent_Identity=35.0453172205438, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1788044, Length=323, Percent_Identity=31.8885448916409, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789759, Length=331, Percent_Identity=31.1178247734139, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI145693181, Length=305, Percent_Identity=30.8196721311475, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787560, Length=306, Percent_Identity=32.3529411764706, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786991, Length=335, Percent_Identity=28.3582089552239, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144271, Length=334, Percent_Identity=28.1437125748503, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71992977, Length=341, Percent_Identity=29.3255131964809, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25144274, Length=140, Percent_Identity=34.2857142857143, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323470, Length=410, Percent_Identity=27.8048780487805, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324432, Length=323, Percent_Identity=25.3869969040248, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356575, Length=330, Percent_Identity=28.4848484848485, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21357415, Length=323, Percent_Identity=27.2445820433437, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46493; Mature: 46362

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQLTRSAELFEKAKQFIPGGVNSPVRAFKSVGGTPIYMAKGQGAYMTDVDGNTYLDYVG
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCEEEHCCC
SWGPFILGSMHPRITAALEHTLTKIGTSFGTPIEMEIEIAELLCQIVPSIEMVRMVNSGT
CCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCHHHHHHHCCCH
EATMSAVRLARGYTSRDKIIKFEGCYHGHGDSFLIKAGSGALTLGAPDSPGVTKGTANDT
HHHHHHHHHHHCCCCCCCEEEEECEECCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCC
LNAKYNDIESVRLLVNENKGNIAAIIIEPVAGNTGVIPAKPGFLQALRDLCTEEGIVLIF
CCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEE
DEVMCGFRVALGGAQERYGVTPDLTTMGKIIGGGLPVGAFGGKREIMERIAPLGDVYQAG
HHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHCC
TLSGNPLALTAGLETLKILRDENPYPELERKAAFLEEGFRNNMNKLGLNYVQNRVGSMAC
CCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEE
LFFTETPVTDYDTAVTADLKKYGTYYHAMLDQGIYLAPSQFEAMFTSAVMTDEDLEKTVK
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHCCHHHHHHHHH
ANYVALQATQK
CCEEEEEECCC
>Mature Secondary Structure 
PQLTRSAELFEKAKQFIPGGVNSPVRAFKSVGGTPIYMAKGQGAYMTDVDGNTYLDYVG
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCEEEHCCC
SWGPFILGSMHPRITAALEHTLTKIGTSFGTPIEMEIEIAELLCQIVPSIEMVRMVNSGT
CCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCHHHHHHHCCCH
EATMSAVRLARGYTSRDKIIKFEGCYHGHGDSFLIKAGSGALTLGAPDSPGVTKGTANDT
HHHHHHHHHHHCCCCCCCEEEEECEECCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCC
LNAKYNDIESVRLLVNENKGNIAAIIIEPVAGNTGVIPAKPGFLQALRDLCTEEGIVLIF
CCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEE
DEVMCGFRVALGGAQERYGVTPDLTTMGKIIGGGLPVGAFGGKREIMERIAPLGDVYQAG
HHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHCC
TLSGNPLALTAGLETLKILRDENPYPELERKAAFLEEGFRNNMNKLGLNYVQNRVGSMAC
CCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEE
LFFTETPVTDYDTAVTADLKKYGTYYHAMLDQGIYLAPSQFEAMFTSAVMTDEDLEKTVK
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHCCHHHHHHHHH
ANYVALQATQK
CCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA