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The map label for this gene is prfA

Identifier: 194332966

GI number: 194332966

Start: 123084

End: 124157

Strand: Reverse

Name: prfA

Synonym: Paes_0119

Alternate gene names: 194332966

Gene position: 124157-123084 (Counterclockwise)

Preceding gene: 194332967

Following gene: 194332965

Centisome position: 4.94

GC content: 55.03

Gene sequence:

>1074_bases
ATGCTGAATAAACTCGAATCAGCAAAAGACCACTATATCCGTCTTGAAGAACAGCTCTCCGATCCGGACATCATCGGAGA
CCAGCAACGGTTCAGAAAACTCAACAAGGAGTACAGCGACCTCAAGGAGATCGTCCAGGCTTACGACCGCTATATATCGA
ACCGCCGCCAGATCGAAGAGGTCCTCGGCATGCTCGCCAAAGAATCGGACCCGGAGATGAAAGCCCTTGCCGAGGAGGAA
TACAACGAACTGCTGGCCGACCAGCCAGAGCTTGAACAGCAGCTTAAACTCCTTCTGCTTCCCAAAGATGAAGCGGACTC
GCGAAACGTCATCATGGAAATTCGGGCCGGTACCGGCGGCGACGAAGCGGCCCTGTTTGCAACAGACCTCCTGAGAATGT
ACCAGCGTTTTGCGGAGCGACGAGGCTGGAAGTGTGAACTGCTTGAATTCAACGAAGCCAACACGCCCGGCGCCTGCAAA
GAAGCCTCTATGGCAATAAGCGGTCACGACGTGTATGGCATCATGAAATTTGAAAGCGGCGTACACCGGGTCCAGCGCGT
GCCGGAAACAGAAACCCAGGGAAGAATCCACACCTCGGCAGCAAGCGTAGCCGTTCTACCTGAAGCCGAAGAGGTCGACA
TCGAAATCCGCAAGGACGACCTGCGTGTGGACACCTTCCGAAGTGGCGGGAAAGGCGGTCAGAACGTCAACAAGGTGGAA
ACAGCGGTCCGTATCACCCATGCGCCCAGCGGCATCGTCGTTGCCTGCCAGGAGGAACGCTCACAGCTCCAGAACCGCGA
GCGCGCCATGAAAATGCTCAGAGCAAAGCTCTATGACAGAGAGCTGGCGGACAAGAACAAAGAACGGGCAGATCTGAGGA
AATCGATGGTCTCCTCCGGCGACAGAAGCGCAAAAATCCGGACGTACAACTTCCCTCAATCCCGCGTCACCGATCACCGC
ATCGGATATACGTCGCATGCACTTCCGCAAATCCTTGACGGAAATCTCGATGATCTTATCGAGGCACTCAAGCTCTACGA
ACAGACAGCACGGCTTCAGGAACAACAGCAGTAG

Upstream 100 bases:

>100_bases
ACAGCAAGTCGGCATGGCACTCTTACTGACGCTCTTCATGTTCTTCATCATTAATGATATCATTAACCCTTAACCGGACC
GGAAGACACCGCCATTATTC

Downstream 100 bases:

>100_bases
CTGCTTGATTGCTACCGGTTACAGAAGGCTACGATGCAATTATTGCAGATTCTCCGTATATTAAAAGTCTGACTCTTACA
CAAGAACCAATGATATCCTG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MLNKLESAKDHYIRLEEQLSDPDIIGDQQRFRKLNKEYSDLKEIVQAYDRYISNRRQIEEVLGMLAKESDPEMKALAEEE
YNELLADQPELEQQLKLLLLPKDEADSRNVIMEIRAGTGGDEAALFATDLLRMYQRFAERRGWKCELLEFNEANTPGACK
EASMAISGHDVYGIMKFESGVHRVQRVPETETQGRIHTSAASVAVLPEAEEVDIEIRKDDLRVDTFRSGGKGGQNVNKVE
TAVRITHAPSGIVVACQEERSQLQNRERAMKMLRAKLYDRELADKNKERADLRKSMVSSGDRSAKIRTYNFPQSRVTDHR
IGYTSHALPQILDGNLDDLIEALKLYEQTARLQEQQQ

Sequences:

>Translated_357_residues
MLNKLESAKDHYIRLEEQLSDPDIIGDQQRFRKLNKEYSDLKEIVQAYDRYISNRRQIEEVLGMLAKESDPEMKALAEEE
YNELLADQPELEQQLKLLLLPKDEADSRNVIMEIRAGTGGDEAALFATDLLRMYQRFAERRGWKCELLEFNEANTPGACK
EASMAISGHDVYGIMKFESGVHRVQRVPETETQGRIHTSAASVAVLPEAEEVDIEIRKDDLRVDTFRSGGKGGQNVNKVE
TAVRITHAPSGIVVACQEERSQLQNRERAMKMLRAKLYDRELADKNKERADLRKSMVSSGDRSAKIRTYNFPQSRVTDHR
IGYTSHALPQILDGNLDDLIEALKLYEQTARLQEQQQ
>Mature_357_residues
MLNKLESAKDHYIRLEEQLSDPDIIGDQQRFRKLNKEYSDLKEIVQAYDRYISNRRQIEEVLGMLAKESDPEMKALAEEE
YNELLADQPELEQQLKLLLLPKDEADSRNVIMEIRAGTGGDEAALFATDLLRMYQRFAERRGWKCELLEFNEANTPGACK
EASMAISGHDVYGIMKFESGVHRVQRVPETETQGRIHTSAASVAVLPEAEEVDIEIRKDDLRVDTFRSGGKGGQNVNKVE
TAVRITHAPSGIVVACQEERSQLQNRERAMKMLRAKLYDRELADKNKERADLRKSMVSSGDRSAKIRTYNFPQSRVTDHR
IGYTSHALPQILDGNLDDLIEALKLYEQTARLQEQQQ

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=293, Percent_Identity=46.7576791808874, Blast_Score=271, Evalue=5e-73,
Organism=Homo sapiens, GI34577120, Length=349, Percent_Identity=38.3954154727794, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI166795305, Length=195, Percent_Identity=43.5897435897436, Blast_Score=172, Evalue=3e-43,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=48.0555555555556, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI2367172, Length=340, Percent_Identity=40.8823529411765, Blast_Score=229, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17542784, Length=296, Percent_Identity=40.2027027027027, Blast_Score=185, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=328, Percent_Identity=41.7682926829268, Blast_Score=244, Evalue=1e-65,
Organism=Drosophila melanogaster, GI19921226, Length=313, Percent_Identity=41.85303514377, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_PROA2 (B4S385)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002014826.1
- ProteinModelPortal:   B4S385
- SMR:   B4S385
- GeneID:   6458478
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_0119
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40811; Mature: 40811

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKLESAKDHYIRLEEQLSDPDIIGDQQRFRKLNKEYSDLKEIVQAYDRYISNRRQIEE
CCCCHHHHHHHHEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLGMLAKESDPEMKALAEEEYNELLADQPELEQQLKLLLLPKDEADSRNVIMEIRAGTGG
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHEEECCCCCCCCCCEEEEEECCCCC
DEAALFATDLLRMYQRFAERRGWKCELLEFNEANTPGACKEASMAISGHDVYGIMKFESG
CHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHCCEEECCCCEEEEEHHHHH
VHRVQRVPETETQGRIHTSAASVAVLPEAEEVDIEIRKDDLRVDTFRSGGKGGQNVNKVE
HHHHHHCCCCCCCCEEEECCCCEEECCCCCCCEEEEECCCCEEECCCCCCCCCCCHHHHH
TAVRITHAPSGIVVACQEERSQLQNRERAMKMLRAKLYDRELADKNKERADLRKSMVSSG
HHHEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
DRSAKIRTYNFPQSRVTDHRIGYTSHALPQILDGNLDDLIEALKLYEQTARLQEQQQ
CCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLNKLESAKDHYIRLEEQLSDPDIIGDQQRFRKLNKEYSDLKEIVQAYDRYISNRRQIEE
CCCCHHHHHHHHEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLGMLAKESDPEMKALAEEEYNELLADQPELEQQLKLLLLPKDEADSRNVIMEIRAGTGG
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHEEECCCCCCCCCCEEEEEECCCCC
DEAALFATDLLRMYQRFAERRGWKCELLEFNEANTPGACKEASMAISGHDVYGIMKFESG
CHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHCCEEECCCCEEEEEHHHHH
VHRVQRVPETETQGRIHTSAASVAVLPEAEEVDIEIRKDDLRVDTFRSGGKGGQNVNKVE
HHHHHHCCCCCCCCEEEECCCCEEECCCCCCCEEEEECCCCEEECCCCCCCCCCCHHHHH
TAVRITHAPSGIVVACQEERSQLQNRERAMKMLRAKLYDRELADKNKERADLRKSMVSSG
HHHEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
DRSAKIRTYNFPQSRVTDHRIGYTSHALPQILDGNLDDLIEALKLYEQTARLQEQQQ
CCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA