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The map label for this gene is gyrB [H]

Identifier: 194332868

GI number: 194332868

Start: 17535

End: 19469

Strand: Reverse

Name: gyrB [H]

Synonym: Paes_0015

Alternate gene names: 194332868

Gene position: 19469-17535 (Counterclockwise)

Preceding gene: 194332869

Following gene: 194332860

Centisome position: 0.77

GC content: 50.28

Gene sequence:

>1935_bases
ATGTCAGAAACCCAGACTCCGTCCGCTTCGTCATCATATCTGGCGACCAATATTCAGATTCTCGACGGTATAGAGCATGT
CCGTAAACGCCCGGCGATGTACATCGGCGACATCAACGTCCGTGGATTGCATCACCTCATCTATGAGATCGTTGACAACT
CGATCGATGAAACGCTTGCAGGATATAATGATACGATTGAAATGTCACTCAATGACGACGGCTCTGTCACGGTCTCGGAC
AACGGCCGCGGCATTCCGGTAGACATGCATCCCCAGAAAAAGAAGTCTGCGCTTGAACTGGTCATGACAGTTATCGGTGC
GGGCGGAAAGTTCGACAAGGGCGCTTATAAAGTGTCCGGCGGTCTGCACGGCGTCGGCGCCTCGGTGGTCAACGCGCTGT
CGGAGTGGTGCGAGGTCGAGGTGAGCAGGGACGGCAAAGTCTGGTATCAGCGATACCACAGAGGTGTCCCCCAGTGCGAA
GTCAAAATTATCGGTGAAACCGATAAAACCGGCACAAAAACCACATTCATGCCTGATGCTTCGATTTTCAAGACCACTGA
ATTCCGAAAAGACGTCATTATCGACCGCATGAGAGAACTGGCGTTTCTCAACAGTGTACTCAAGATTGTCGTCAAAGACA
CCGACGATTCGGAAGAAACCTTTCATTATGAAGGCGGCATCAAAGAGTTTGTCACCTATACCGATCATAACAGGATTGCG
CTTGTCAAGGAACCAGTTTACCTCTACGGAGAGCGTGACACGACAGTGGTCGAAATAGCACTGCAGTACAACGACTCCTA
TCAGGAAAACGTCTTCAGCTACGTCAATAACATCAATACCCACGAAGGGGGTACGCATATGACTGGTTTCCGCAAGGCGC
TGACCAGAACACTGAATGCATACGCCCAGAAAAACGACCTGCTTAAAAATCTCAAGCTCTCATTGACAGGTGATGATTTC
AAGGAAGGACTGACCGCTATCATCTCCGTGAAAGTTGCCGAACCTCAATTCGAAGGCCAGACAAAAACAAAACTCGGCAA
CTCTGAAACACAGAGTATTGTCGAAAGCATCGTCAATGAACAGCTTGCCGAGTTTCTCGAAAGCAATCCCAATGTGCTCA
AGACCATCATCGAGAAAGTCAAAAGCGCAGCGCTTTCAAGAGATGCTGCAAGAAAAGCAAAGGAGCTGACCCGACGCAAG
TCCGTGCTGGAAAGCACCGGCCTGCCGGGCAAACTTGCTGACTGCTCAATCAACGATCCGGAACACTGCGAACTTTACAT
CGTCGAGGGTGACTCGGCAGGCGGCAGTGCCAAGCAGGGACGGGACCGGAGTTTTCAGGCGATCCTTCCGCTGAAGGGCA
AAATTCTCAATGTGGAAAAGGCCCGGCTGCACAAGATGCTTGAAAACGAAGAAATCAAGACCATTATCCTGGCACTCGGC
ACAAACTTCGGAGAAGATGAGTTTATGGCCGACAAACTTCGCTACGGCAAGATCATCATTATGACTGATGCCGATGTGGA
TGGCGCCCATATCAGAACGCTCCTGCTGACCTTTTTCTTCCGCCATATGCGCCCCCTCATTGAAGCAGGCAAAGTCTTTA
TCGCACAGCCGCCTCTCTACCTTATCAAAGCAGGCAAGGAGCAGAAATACGCCTGGGACGAAGATGAGCGAACAACCATC
ATGGAGATGATGAAAAAATCACAGAAAGGCAAAACCAATATCAATATTCAGCGATATAAAGGTCTTGGTGAAATGAACCC
TGAACAGCTCTGGAGCACGACGATGGATCCTGAGCACCGCTCGCTGCTTCAGGTAACGGTGGAAAACGCAATGGAAGCGG
ACCAGGTCTTCTCGACCCTGATGGGCGACAAAGTTGATCCGCGCCGTGAATTCATTGAAAAGAACGCCCGGTATGTCCGG
CGATTAGATGTCTGA

Upstream 100 bases:

>100_bases
GCGGAATGACGCCGCCAACATAGCATATAACGGTAATTTTCGCTATCTTTGAAATCAGATTTTTTAACGTATACAACGTT
TTTTTCCAGGAAAATTCACT

Downstream 100 bases:

>100_bases
ATCAAGAATGTTCACATACACCTCTTTTTTCAATCCCTCTATCCATGTTGTAAACATCACCTTCTGCTTTTCATCGATCG
CCAGCTTTTCAAGTCTGGCG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 644; Mature: 643

Protein sequence:

>644_residues
MSETQTPSASSSYLATNIQILDGIEHVRKRPAMYIGDINVRGLHHLIYEIVDNSIDETLAGYNDTIEMSLNDDGSVTVSD
NGRGIPVDMHPQKKKSALELVMTVIGAGGKFDKGAYKVSGGLHGVGASVVNALSEWCEVEVSRDGKVWYQRYHRGVPQCE
VKIIGETDKTGTKTTFMPDASIFKTTEFRKDVIIDRMRELAFLNSVLKIVVKDTDDSEETFHYEGGIKEFVTYTDHNRIA
LVKEPVYLYGERDTTVVEIALQYNDSYQENVFSYVNNINTHEGGTHMTGFRKALTRTLNAYAQKNDLLKNLKLSLTGDDF
KEGLTAIISVKVAEPQFEGQTKTKLGNSETQSIVESIVNEQLAEFLESNPNVLKTIIEKVKSAALSRDAARKAKELTRRK
SVLESTGLPGKLADCSINDPEHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALG
TNFGEDEFMADKLRYGKIIIMTDADVDGAHIRTLLLTFFFRHMRPLIEAGKVFIAQPPLYLIKAGKEQKYAWDEDERTTI
MEMMKKSQKGKTNINIQRYKGLGEMNPEQLWSTTMDPEHRSLLQVTVENAMEADQVFSTLMGDKVDPRREFIEKNARYVR
RLDV

Sequences:

>Translated_644_residues
MSETQTPSASSSYLATNIQILDGIEHVRKRPAMYIGDINVRGLHHLIYEIVDNSIDETLAGYNDTIEMSLNDDGSVTVSD
NGRGIPVDMHPQKKKSALELVMTVIGAGGKFDKGAYKVSGGLHGVGASVVNALSEWCEVEVSRDGKVWYQRYHRGVPQCE
VKIIGETDKTGTKTTFMPDASIFKTTEFRKDVIIDRMRELAFLNSVLKIVVKDTDDSEETFHYEGGIKEFVTYTDHNRIA
LVKEPVYLYGERDTTVVEIALQYNDSYQENVFSYVNNINTHEGGTHMTGFRKALTRTLNAYAQKNDLLKNLKLSLTGDDF
KEGLTAIISVKVAEPQFEGQTKTKLGNSETQSIVESIVNEQLAEFLESNPNVLKTIIEKVKSAALSRDAARKAKELTRRK
SVLESTGLPGKLADCSINDPEHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALG
TNFGEDEFMADKLRYGKIIIMTDADVDGAHIRTLLLTFFFRHMRPLIEAGKVFIAQPPLYLIKAGKEQKYAWDEDERTTI
MEMMKKSQKGKTNINIQRYKGLGEMNPEQLWSTTMDPEHRSLLQVTVENAMEADQVFSTLMGDKVDPRREFIEKNARYVR
RLDV
>Mature_643_residues
SETQTPSASSSYLATNIQILDGIEHVRKRPAMYIGDINVRGLHHLIYEIVDNSIDETLAGYNDTIEMSLNDDGSVTVSDN
GRGIPVDMHPQKKKSALELVMTVIGAGGKFDKGAYKVSGGLHGVGASVVNALSEWCEVEVSRDGKVWYQRYHRGVPQCEV
KIIGETDKTGTKTTFMPDASIFKTTEFRKDVIIDRMRELAFLNSVLKIVVKDTDDSEETFHYEGGIKEFVTYTDHNRIAL
VKEPVYLYGERDTTVVEIALQYNDSYQENVFSYVNNINTHEGGTHMTGFRKALTRTLNAYAQKNDLLKNLKLSLTGDDFK
EGLTAIISVKVAEPQFEGQTKTKLGNSETQSIVESIVNEQLAEFLESNPNVLKTIIEKVKSAALSRDAARKAKELTRRKS
VLESTGLPGKLADCSINDPEHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALGT
NFGEDEFMADKLRYGKIIIMTDADVDGAHIRTLLLTFFFRHMRPLIEAGKVFIAQPPLYLIKAGKEQKYAWDEDERTTIM
EMMKKSQKGKTNINIQRYKGLGEMNPEQLWSTTMDPEHRSLLQVTVENAMEADQVFSTLMGDKVDPRREFIEKNARYVRR
LDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=701, Percent_Identity=26.5335235378031, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI19913406, Length=622, Percent_Identity=26.0450160771704, Blast_Score=135, Evalue=9e-32,
Organism=Escherichia coli, GI48994957, Length=549, Percent_Identity=55.1912568306011, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1789408, Length=633, Percent_Identity=39.1785150078989, Blast_Score=431, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI212645845, Length=597, Percent_Identity=26.1306532663317, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17535065, Length=678, Percent_Identity=24.9262536873156, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI212645657, Length=236, Percent_Identity=29.6610169491525, Blast_Score=73, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=565, Percent_Identity=26.1946902654867, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI17136538, Length=616, Percent_Identity=25.8116883116883, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72390; Mature: 72259

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETQTPSASSSYLATNIQILDGIEHVRKRPAMYIGDINVRGLHHLIYEIVDNSIDETLA
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCHHHHHHC
GYNDTIEMSLNDDGSVTVSDNGRGIPVDMHPQKKKSALELVMTVIGAGGKFDKGAYKVSG
CCCCEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECC
GLHGVGASVVNALSEWCEVEVSRDGKVWYQRYHRGVPQCEVKIIGETDKTGTKTTFMPDA
CCCCHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCCC
SIFKTTEFRKDVIIDRMRELAFLNSVLKIVVKDTDDSEETFHYEGGIKEFVTYTDHNRIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEECCCHHHHHEECCCCEEE
LVKEPVYLYGERDTTVVEIALQYNDSYQENVFSYVNNINTHEGGTHMTGFRKALTRTLNA
EEECCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
YAQKNDLLKNLKLSLTGDDFKEGLTAIISVKVAEPQFEGQTKTKLGNSETQSIVESIVNE
HHHHHHHHHHCEEEECCCHHHHHHEEEEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHH
QLAEFLESNPNVLKTIIEKVKSAALSRDAARKAKELTRRKSVLESTGLPGKLADCSINDP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALG
CCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEECHHHHHHHHHHCCCCHHEEEEEEC
TNFGEDEFMADKLRYGKIIIMTDADVDGAHIRTLLLTFFFRHMRPLIEAGKVFIAQPPLY
CCCCCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEE
LIKAGKEQKYAWDEDERTTIMEMMKKSQKGKTNINIQRYKGLGEMNPEQLWSTTMDPEHR
EEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEHEECCCCCCCHHHHHCCCCCHHHH
SLLQVTVENAMEADQVFSTLMGDKVDPRREFIEKNARYVRRLDV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SETQTPSASSSYLATNIQILDGIEHVRKRPAMYIGDINVRGLHHLIYEIVDNSIDETLA
CCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCHHHHHHC
GYNDTIEMSLNDDGSVTVSDNGRGIPVDMHPQKKKSALELVMTVIGAGGKFDKGAYKVSG
CCCCEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECC
GLHGVGASVVNALSEWCEVEVSRDGKVWYQRYHRGVPQCEVKIIGETDKTGTKTTFMPDA
CCCCHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCCC
SIFKTTEFRKDVIIDRMRELAFLNSVLKIVVKDTDDSEETFHYEGGIKEFVTYTDHNRIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEECCCHHHHHEECCCCEEE
LVKEPVYLYGERDTTVVEIALQYNDSYQENVFSYVNNINTHEGGTHMTGFRKALTRTLNA
EEECCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
YAQKNDLLKNLKLSLTGDDFKEGLTAIISVKVAEPQFEGQTKTKLGNSETQSIVESIVNE
HHHHHHHHHHCEEEECCCHHHHHHEEEEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHH
QLAEFLESNPNVLKTIIEKVKSAALSRDAARKAKELTRRKSVLESTGLPGKLADCSINDP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALG
CCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEECHHHHHHHHHHCCCCHHEEEEEEC
TNFGEDEFMADKLRYGKIIIMTDADVDGAHIRTLLLTFFFRHMRPLIEAGKVFIAQPPLY
CCCCCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEE
LIKAGKEQKYAWDEDERTTIMEMMKKSQKGKTNINIQRYKGLGEMNPEQLWSTTMDPEHR
EEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEHEECCCCCCCHHHHHCCCCCHHHH
SLLQVTVENAMEADQVFSTLMGDKVDPRREFIEKNARYVRRLDV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]